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The map label for this gene is queE [H]

Identifier: 119715336

GI number: 119715336

Start: 1157792

End: 1158532

Strand: Reverse

Name: queE [H]

Synonym: Noca_1098

Alternate gene names: 119715336

Gene position: 1158532-1157792 (Counterclockwise)

Preceding gene: 119715337

Following gene: 119715335

Centisome position: 23.24

GC content: 63.29

Gene sequence:

>741_bases
TTGTCATCGAAAGCTGACTCTACGTGGCGCGGCGTCGGACGCCCCGGGCGAGGAGAACTGCTGATCAGTGAGATCTTCGG
CCCAACGCTGCAGGGGGAGGGTCCAAGCGCGGGCAAATCGGCCGCATTCGTGCGACTCGGCGCGTGCAATCTTGCGTGCG
TCTGGTGCGATACCTCCTACACATGGGACAGCAGTCGTTACGATCTCGCGTCGGAGCTCGTTGCCAAACCGACGGCCGAA
GTAGCCGACAAAGCGCTCTCATTCGGGGTCCCGCTTGTCGTCATCACCGGTGGCGAGCCAGCACTCCAAGCCGTCGAAGC
TGCCCGTCTCGCGGAGGCCGTCACTCGGAGCGGCAGCGCCGTTGAGCTCGAGACTAGCGGCTCGCTTCCATTGGGCCCGC
TCGCGGATGCGGTGCGACTGATCGTCGTTAGCCCGAAGCTGGCGAACGCCGGTGGCCGACCTCAGGCACGTCTTCGTTGG
CCGGTCTTAGAGGCGATCTCCGTCCTCCCTCATTCCGTTCTCAAATTCGTCGTCGCGTCGCCAGAGGAATTGGCCGAGGT
TGACGAGATCACATCCCGGCTACAAACGAAGCCTGAAAGGGTCTGGATCATGCCAGAAGGGACGGAGCGGGAAACCGTGC
TGGCAAGGATGGCTGAGCTCACGGGTCCGGTCGCATCCAGGGGTTGGTCGCTGAGCAGTCGGCTCCAGGTCATCATGTGG
GGGAATGACCGGGGTCGATGA

Upstream 100 bases:

>100_bases
CCAACTGCCGAGTTGGTCGCTGTCCATATCGGGGAGCGGCTCGACGCCGCAGGGTTGTCGTGGACCATGCTGCGGTTGTG
GGAGACGGAGGATGGGTCCG

Downstream 100 bases:

>100_bases
GGGGATCCAACATCTGCTTGCGAGTTGGTCCATCGTGGGCCCTCGGTTCATGACATTGTGCTGACGTGAAGCGCCCAGCG
ACTGACATTTGCGTCTTGCT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSSKADSTWRGVGRPGRGELLISEIFGPTLQGEGPSAGKSAAFVRLGACNLACVWCDTSYTWDSSRYDLASELVAKPTAE
VADKALSFGVPLVVITGGEPALQAVEAARLAEAVTRSGSAVELETSGSLPLGPLADAVRLIVVSPKLANAGGRPQARLRW
PVLEAISVLPHSVLKFVVASPEELAEVDEITSRLQTKPERVWIMPEGTERETVLARMAELTGPVASRGWSLSSRLQVIMW
GNDRGR

Sequences:

>Translated_246_residues
MSSKADSTWRGVGRPGRGELLISEIFGPTLQGEGPSAGKSAAFVRLGACNLACVWCDTSYTWDSSRYDLASELVAKPTAE
VADKALSFGVPLVVITGGEPALQAVEAARLAEAVTRSGSAVELETSGSLPLGPLADAVRLIVVSPKLANAGGRPQARLRW
PVLEAISVLPHSVLKFVVASPEELAEVDEITSRLQTKPERVWIMPEGTERETVLARMAELTGPVASRGWSLSSRLQVIMW
GNDRGR
>Mature_245_residues
SSKADSTWRGVGRPGRGELLISEIFGPTLQGEGPSAGKSAAFVRLGACNLACVWCDTSYTWDSSRYDLASELVAKPTAEV
ADKALSFGVPLVVITGGEPALQAVEAARLAEAVTRSGSAVELETSGSLPLGPLADAVRLIVVSPKLANAGGRPQARLRWP
VLEAISVLPHSVLKFVVASPEELAEVDEITSRLQTKPERVWIMPEGTERETVLARMAELTGPVASRGWSLSSRLQVIMWG
NDRGR

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=145, Percent_Identity=31.7241379310345, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 26173; Mature: 26042

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKADSTWRGVGRPGRGELLISEIFGPTLQGEGPSAGKSAAFVRLGACNLACVWCDTSY
CCCCCCCCHHCCCCCCCCCEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCC
TWDSSRYDLASELVAKPTAEVADKALSFGVPLVVITGGEPALQAVEAARLAEAVTRSGSA
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCE
VELETSGSLPLGPLADAVRLIVVSPKLANAGGRPQARLRWPVLEAISVLPHSVLKFVVAS
EEEECCCCCCCCHHHHHEEEEEECCHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCC
PEELAEVDEITSRLQTKPERVWIMPEGTERETVLARMAELTGPVASRGWSLSSRLQVIMW
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEE
GNDRGR
CCCCCC
>Mature Secondary Structure 
SSKADSTWRGVGRPGRGELLISEIFGPTLQGEGPSAGKSAAFVRLGACNLACVWCDTSY
CCCCCCCHHCCCCCCCCCEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCC
TWDSSRYDLASELVAKPTAEVADKALSFGVPLVVITGGEPALQAVEAARLAEAVTRSGSA
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCE
VELETSGSLPLGPLADAVRLIVVSPKLANAGGRPQARLRWPVLEAISVLPHSVLKFVVAS
EEEECCCCCCCCHHHHHEEEEEECCHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCC
PEELAEVDEITSRLQTKPERVWIMPEGTERETVLARMAELTGPVASRGWSLSSRLQVIMW
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEE
GNDRGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]