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The map label for this gene is paaF [C]

Identifier: 119715102

GI number: 119715102

Start: 894722

End: 895423

Strand: Direct

Name: paaF [C]

Synonym: Noca_0857

Alternate gene names: 119715102

Gene position: 894722-895423 (Clockwise)

Preceding gene: 119715101

Following gene: 119715103

Centisome position: 17.95

GC content: 74.22

Gene sequence:

>702_bases
ATGACCCAGCTGTTGCGCACCGTGGACGAGGGAGTGGTGACCCTCACCCTCCACCGGCCCGAGAGCAGGAACGCCCTCAC
GACCGAGCTGCTGCGCAGCCTCGTCGCGGAGCTGGCCGTCGTGCAGGACGCCCCCGACCTCCGGGTCGTCGTGCTGGCCG
GGGCGGGTGGGGCCTTCTGCGCGGGCGCGGACCTCAAGGAGCTCGGCGCCACGGCATCAGCCGAGGACCGCCAACGGAGG
ATCAGGCTGGTCACCGAGGCGATCGCCCGGCTGCGCAACCTCGAGCAGCCGACGATCGCGGTGGTGACCGGTGCCGCGTA
CGGCGCCGGCTGGGGCCTGGCGCTGGCCTGCGACCTCACGATCGGGTCCGCGTCGGCGCGGTTCAGCCTTCCTGAGGTGC
CCAAGGGGCTGCGCCTGCCCGCCGCGATCACTGCGCGGCTCGTCGAGGTGGTCGGCCCGGTCCGGGCTGCCGACATCGCC
CTCGGTGGCGGCACCTACGGCCCCGACGAGGGGGTCGCCCTCGGGTGGCTCGCGCACGCCCTGCCCGACGACGAGTCCGC
ACACGCGCACGCCCGCGGCTTCGCTCGCCGCCTCGCGACGAGCCCGCGCTCGTCGGTCGCCCACGTCAAGCAGGTGCTGA
GGCGGGGTTCGCCCCACGACCTCACGCCGCCCCCCGAGTACGCCTGGATTGAGGAGAAGTGA

Upstream 100 bases:

>100_bases
AGTTCTACGTCGGGGCCCCGCTCGGGCCCGACCCGCACGAGGCCGCCGGGCTCCTGATCGATCAGGTCATCCCGCAGGTC
TGGCCCGGACGAGCCTCGCG

Downstream 100 bases:

>100_bases
CCGAGGACATGACCGATTCCGACCAGCTGCGGGAGTTCGTGCGCCAGAGCGTGCGCGCTTTCGTGCACAAGGAGTGCGAC
GAGTGGCGTTGCCTCGAGTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTQLLRTVDEGVVTLTLHRPESRNALTTELLRSLVAELAVVQDAPDLRVVVLAGAGGAFCAGADLKELGATASAEDRQRR
IRLVTEAIARLRNLEQPTIAVVTGAAYGAGWGLALACDLTIGSASARFSLPEVPKGLRLPAAITARLVEVVGPVRAADIA
LGGGTYGPDEGVALGWLAHALPDDESAHAHARGFARRLATSPRSSVAHVKQVLRRGSPHDLTPPPEYAWIEEK

Sequences:

>Translated_233_residues
MTQLLRTVDEGVVTLTLHRPESRNALTTELLRSLVAELAVVQDAPDLRVVVLAGAGGAFCAGADLKELGATASAEDRQRR
IRLVTEAIARLRNLEQPTIAVVTGAAYGAGWGLALACDLTIGSASARFSLPEVPKGLRLPAAITARLVEVVGPVRAADIA
LGGGTYGPDEGVALGWLAHALPDDESAHAHARGFARRLATSPRSSVAHVKQVLRRGSPHDLTPPPEYAWIEEK
>Mature_232_residues
TQLLRTVDEGVVTLTLHRPESRNALTTELLRSLVAELAVVQDAPDLRVVVLAGAGGAFCAGADLKELGATASAEDRQRRI
RLVTEAIARLRNLEQPTIAVVTGAAYGAGWGLALACDLTIGSASARFSLPEVPKGLRLPAAITARLVEVVGPVRAADIAL
GGGTYGPDEGVALGWLAHALPDDESAHAHARGFARRLATSPRSSVAHVKQVLRRGSPHDLTPPPEYAWIEEK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=215, Percent_Identity=28.8372093023256, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI194097323, Length=217, Percent_Identity=28.110599078341, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI70995211, Length=185, Percent_Identity=28.6486486486486, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=31.6939890710383, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=191, Percent_Identity=30.8900523560209, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI37594471, Length=129, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=129, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI157694516, Length=214, Percent_Identity=21.0280373831776, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI213417737, Length=214, Percent_Identity=21.0280373831776, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=218, Percent_Identity=33.4862385321101, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787660, Length=224, Percent_Identity=33.9285714285714, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI221142681, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1788597, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=218, Percent_Identity=29.3577981651376, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=28.3783783783784, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=233, Percent_Identity=24.8927038626609, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=183, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=176, Percent_Identity=29.5454545454545, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=206, Percent_Identity=28.6407766990291, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=163, Percent_Identity=30.0613496932515, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=177, Percent_Identity=35.0282485875706, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=177, Percent_Identity=35.0282485875706, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653139, Length=223, Percent_Identity=28.6995515695067, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=26.3636363636364, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922422, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 24534; Mature: 24403

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLLRTVDEGVVTLTLHRPESRNALTTELLRSLVAELAVVQDAPDLRVVVLAGAGGAFC
CCHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
AGADLKELGATASAEDRQRRIRLVTEAIARLRNLEQPTIAVVTGAAYGAGWGLALACDLT
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEE
IGSASARFSLPEVPKGLRLPAAITARLVEVVGPVRAADIALGGGTYGPDEGVALGWLAHA
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCHHHHHHHHH
LPDDESAHAHARGFARRLATSPRSSVAHVKQVLRRGSPHDLTPPPEYAWIEEK
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQLLRTVDEGVVTLTLHRPESRNALTTELLRSLVAELAVVQDAPDLRVVVLAGAGGAFC
CHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
AGADLKELGATASAEDRQRRIRLVTEAIARLRNLEQPTIAVVTGAAYGAGWGLALACDLT
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEE
IGSASARFSLPEVPKGLRLPAAITARLVEVVGPVRAADIALGGGTYGPDEGVALGWLAHA
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCHHHHHHHHH
LPDDESAHAHARGFARRLATSPRSSVAHVKQVLRRGSPHDLTPPPEYAWIEEK
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA