BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is braF [H]

Identifier: 119715099

GI number: 119715099

Start: 892224

End: 892976

Strand: Direct

Name: braF [H]

Synonym: Noca_0854

Alternate gene names: 119715099

Gene position: 892224-892976 (Clockwise)

Preceding gene: 119715098

Following gene: 119715100

Centisome position: 17.9

GC content: 71.05

Gene sequence:

>753_bases
ATGAGCACCGTCCTCGAGCTCGACGCGATCTGCAAGCAGATCGGGGGCGTCCACATCAACCGGGACGTGTCCGTCCGGCT
CGCTCCGGGTGAGCGGCGGGCCCTGGTGGGACCCAACGGCGCGGGCAAGACGACCCTGATGAACATGGCCGCCGGAGTCC
TGCGGCCGACGTCCGGGACCGTCCGCCTGCGCGGCCGGGACGTGACCTCGATGCCGGTGCACCGTCGTGCCCGGTGCGGC
CTCGCGCGGACCTTCCAGATCACCAACCTCTTCCCGGCCATGACGGTGGCCCAGAACCTCGCGATCGCCGCTCAGGTCGG
GAGCCCCCGCCGGTTCGACCCGGTGCGGCCGTGGTCGACGGCCCGCGCCGTCTGGTCCCACGTCGACGGCCTGCTCGAGC
GGGGCGGGCTGACCGCGATCCGGGACATCCAGGTCGGGCAGCTGGCGTACGGCCAGCAGCGGCGTCTGGAGATCGTGGCC
GCCGTCGCCCAGCCGTGCTCGGTGATCCTGCTCGACGAGCCGGGGGCAGGACTGAGCAGCGAGGAGACCGAGGAGCTGCT
CGCCCTGGTCTTCGGCCTCGATCCCGAGCTCGCGGTCATGTTCATCGACCACGACGTGGAGCTGGCGTTCCGGATGGCCA
CCCACGTGACCCTGCTCAACCTCGGCGAGGTCGTCGCCGAGGGCAGCCCCGAGGACGTACGCGCGATGCCGATCATGTCC
GAGATCTACCTGGGGGTGGACGACCATGCTTGA

Upstream 100 bases:

>100_bases
CCACCGACGAGGGTGCGACGCCGGCCCCCGACGCCGGCACCGGCGCGAGTCCCAGCTGCGGCGCGGTTGCGCTCGCCGCC
GTACCGGTCCAGGAGGGCCC

Downstream 100 bases:

>100_bases
GGTCGAGAACCTGAGGGCGACATACGGCGCGGTCCAGGCCGTCCGCGGCGCCAGCTTCCGCGTGGCCGCCGGGGAGGTGC
TGGCCGTCGTCGGCCCCAAC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSTVLELDAICKQIGGVHINRDVSVRLAPGERRALVGPNGAGKTTLMNMAAGVLRPTSGTVRLRGRDVTSMPVHRRARCG
LARTFQITNLFPAMTVAQNLAIAAQVGSPRRFDPVRPWSTARAVWSHVDGLLERGGLTAIRDIQVGQLAYGQQRRLEIVA
AVAQPCSVILLDEPGAGLSSEETEELLALVFGLDPELAVMFIDHDVELAFRMATHVTLLNLGEVVAEGSPEDVRAMPIMS
EIYLGVDDHA

Sequences:

>Translated_250_residues
MSTVLELDAICKQIGGVHINRDVSVRLAPGERRALVGPNGAGKTTLMNMAAGVLRPTSGTVRLRGRDVTSMPVHRRARCG
LARTFQITNLFPAMTVAQNLAIAAQVGSPRRFDPVRPWSTARAVWSHVDGLLERGGLTAIRDIQVGQLAYGQQRRLEIVA
AVAQPCSVILLDEPGAGLSSEETEELLALVFGLDPELAVMFIDHDVELAFRMATHVTLLNLGEVVAEGSPEDVRAMPIMS
EIYLGVDDHA
>Mature_249_residues
STVLELDAICKQIGGVHINRDVSVRLAPGERRALVGPNGAGKTTLMNMAAGVLRPTSGTVRLRGRDVTSMPVHRRARCGL
ARTFQITNLFPAMTVAQNLAIAAQVGSPRRFDPVRPWSTARAVWSHVDGLLERGGLTAIRDIQVGQLAYGQQRRLEIVAA
VAQPCSVILLDEPGAGLSSEETEELLALVFGLDPELAVMFIDHDVELAFRMATHVTLLNLGEVVAEGSPEDVRAMPIMSE
IYLGVDDHA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=197, Percent_Identity=26.9035532994924, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=209, Percent_Identity=24.8803827751196, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI30795238, Length=209, Percent_Identity=24.8803827751196, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31657092, Length=210, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27262624, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27262626, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45446740, Length=225, Percent_Identity=24.4444444444444, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI47078218, Length=225, Percent_Identity=24.4444444444444, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116734710, Length=210, Percent_Identity=28.0952380952381, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI150417984, Length=214, Percent_Identity=25.7009345794392, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI27477115, Length=217, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI27436953, Length=216, Percent_Identity=26.3888888888889, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI153792144, Length=166, Percent_Identity=30.7228915662651, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI11967969, Length=235, Percent_Identity=26.3829787234043, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI21536376, Length=205, Percent_Identity=24.390243902439, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=249, Percent_Identity=36.144578313253, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788761, Length=235, Percent_Identity=34.0425531914894, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790190, Length=228, Percent_Identity=29.8245614035088, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788450, Length=231, Percent_Identity=30.7359307359307, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081791, Length=239, Percent_Identity=28.8702928870293, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789593, Length=239, Percent_Identity=29.2887029288703, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI87081709, Length=239, Percent_Identity=26.7782426778243, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790525, Length=230, Percent_Identity=28.2608695652174, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786253, Length=232, Percent_Identity=30.1724137931034, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788210, Length=223, Percent_Identity=28.6995515695067, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789672, Length=245, Percent_Identity=26.530612244898, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788472, Length=224, Percent_Identity=24.5535714285714, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48995001, Length=223, Percent_Identity=28.2511210762332, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787089, Length=230, Percent_Identity=30.8695652173913, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787712, Length=239, Percent_Identity=26.3598326359833, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787105, Length=236, Percent_Identity=31.7796610169492, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789991, Length=239, Percent_Identity=28.4518828451883, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787370, Length=239, Percent_Identity=26.7782426778243, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786563, Length=221, Percent_Identity=28.9592760180996, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081782, Length=240, Percent_Identity=27.9166666666667, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786803, Length=236, Percent_Identity=27.5423728813559, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082267, Length=237, Percent_Identity=29.1139240506329, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788897, Length=242, Percent_Identity=30.5785123966942, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786872, Length=233, Percent_Identity=29.1845493562232, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788225, Length=247, Percent_Identity=28.7449392712551, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790467, Length=235, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787029, Length=231, Percent_Identity=25.5411255411255, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082268, Length=245, Percent_Identity=26.1224489795918, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081834, Length=233, Percent_Identity=27.0386266094421, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=26.9709543568465, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790162, Length=235, Percent_Identity=25.531914893617, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787792, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786398, Length=221, Percent_Identity=27.1493212669683, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786319, Length=247, Percent_Identity=27.5303643724696, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789873, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790739, Length=251, Percent_Identity=25.8964143426295, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786345, Length=250, Percent_Identity=27.6, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI48994943, Length=213, Percent_Identity=28.169014084507, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789032, Length=244, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786698, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786703, Length=227, Percent_Identity=28.6343612334802, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994997, Length=210, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787182, Length=187, Percent_Identity=28.3422459893048, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789586, Length=225, Percent_Identity=28, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787500, Length=226, Percent_Identity=26.9911504424779, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788506, Length=225, Percent_Identity=26.6666666666667, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17565586, Length=210, Percent_Identity=29.5238095238095, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=27.0531400966184, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=27.0531400966184, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=27.0531400966184, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=236, Percent_Identity=24.1525423728814, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193209708, Length=217, Percent_Identity=27.1889400921659, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=222, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=219, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24666092, Length=225, Percent_Identity=24.8888888888889, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574150, Length=217, Percent_Identity=30.4147465437788, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007328, Length=217, Percent_Identity=30.4147465437788, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648314, Length=236, Percent_Identity=24.5762711864407, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581191, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581189, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581193, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581195, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221512771, Length=225, Percent_Identity=24, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643674, Length=225, Percent_Identity=23.5555555555556, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28574744, Length=222, Percent_Identity=26.5765765765766, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221500365, Length=218, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116007184, Length=218, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28574533, Length=233, Percent_Identity=26.1802575107296, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI85724864, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580555, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85816269, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580553, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076682, Length=213, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28571209, Length=212, Percent_Identity=22.1698113207547, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161076684, Length=213, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581617, Length=213, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581615, Length=213, Percent_Identity=29.5774647887324, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26949; Mature: 26817

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVLELDAICKQIGGVHINRDVSVRLAPGERRALVGPNGAGKTTLMNMAAGVLRPTSGT
CCCHHHHHHHHHHHCCEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCE
VRLRGRDVTSMPVHRRARCGLARTFQITNLFPAMTVAQNLAIAAQVGSPRRFDPVRPWST
EEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHH
ARAVWSHVDGLLERGGLTAIRDIQVGQLAYGQQRRLEIVAAVAQPCSVILLDEPGAGLSS
HHHHHHHHHHHHHCCCCEEEEEEEECHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
EETEELLALVFGLDPELAVMFIDHDVELAFRMATHVTLLNLGEVVAEGSPEDVRAMPIMS
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
EIYLGVDDHA
HHEECCCCCC
>Mature Secondary Structure 
STVLELDAICKQIGGVHINRDVSVRLAPGERRALVGPNGAGKTTLMNMAAGVLRPTSGT
CCHHHHHHHHHHHCCEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCE
VRLRGRDVTSMPVHRRARCGLARTFQITNLFPAMTVAQNLAIAAQVGSPRRFDPVRPWST
EEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHH
ARAVWSHVDGLLERGGLTAIRDIQVGQLAYGQQRRLEIVAAVAQPCSVILLDEPGAGLSS
HHHHHHHHHHHHHCCCCEEEEEEEECHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
EETEELLALVFGLDPELAVMFIDHDVELAFRMATHVTLLNLGEVVAEGSPEDVRAMPIMS
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
EIYLGVDDHA
HHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]