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The map label for this gene is puuB [H]

Identifier: 119714876

GI number: 119714876

Start: 660057

End: 661448

Strand: Direct

Name: puuB [H]

Synonym: Noca_0622

Alternate gene names: 119714876

Gene position: 660057-661448 (Clockwise)

Preceding gene: 119714875

Following gene: 119714877

Centisome position: 13.24

GC content: 65.66

Gene sequence:

>1392_bases
ATGCGCGATCAGCAGGCCCACGCGGCGGCCACCCCCAGCTTCTGGCACGAGGATGCAGACCTCGACTGGGCCCCGAAGGG
ACCCCTCGACGGTGACCTCGATGTCGACGTGGCAATCGTGGGCGGCGGGTACACCGGCCTGTGGACCGCCTACTACCTGG
CGAAGGCGGATCCGAGTCTGCGCATCGCCATCCTCGAGAAGTACTTCGTGGGCTTTGGCGCCTCCGGGCGCAACGGCGGT
TGGTGCTCGGCAATCTTTCCGACGACCCTTCGGAAGGTGGCGGCCGACAGCTCGCGTTCGGCGGCCGTACGGATGCAGGA
GGCGATGTTCGAGACCGTCAAGGAGGTCGGGGAGGTCGTTGTAGCCGAGGGGATCGAATGTGACTTCCAGCCCGGCGGGT
ACGTCTCGGTCGCCCGCAACGAGGCACAATGGGTCAGAAGCCAACGAGAGGTCGATGGCTGGCGTGCCTGGGGATTCGGT
GAGGAACACTTCAGGATGCTCACCCCGGATGAGGCCGCCGAGAGGGTCGCAACCTCACACACTCTCGGCGGGACCTTCAC
TCCCCACTGTGCTGTGGTTCAGCCGGCGAAGCTAGTGCGCGGGCTCGGCGACGTGGTTCGAGCCCTCGGAGTGAGGATCT
TCGAGGCGACGGAGGTTCGGGAGATCCACAGTGGCGGCCTATCGACCGCTCGCGGCACCGTGCGAGCCGATGTCGTCGTC
CGCGCGACCGAGGGCTACACGGCCCAGCTCAAGGGTGCACGCCGCGAGGTCGTCCCGATGTACTCCCTGATGGTCGCGAC
TGCCCCGTTGCCGGAGGAGATCTGGCAGGAGATCGGGCTGCGGGAGCGTGAGACATTCAGCGACAAACGTCACCTGCGGA
TCTACGGTCAGCGCACTCGCGATGGGCGAATAGCTTTCGGCGGGCGCGGTGCGCCGTATCACTTCGGCTCGCGGGTGAAG
CCCGAGTTCGACGACGACAGGCGAGTTCACGAAATGCTCCGCAACATCCTGGTTGACCTGCTCCCGACCATCAGGGGTGT
GGAATTCACTCACGCTTGGGGTGGGAATCTAGGCATCCCGCGAGACTGGTACCCGTCGGTGGGTTACGACCGTAGTTCCG
GAATCGGGCATGCGGGTGGCTACGTCGGCGACGGGGTCGCGACAGCGAACCTTGCGGGCCGGACTCTCGCCGCCGAAATC
CTCGATCTGGAGAACGACGTGCGCTCGCTACCTTGGGTCGGTCGAACCACCCCCCGCTGGGAACCCGAGCCACTACGTTG
GATAGGCGTGAACGCCGGAACCGCCGCCTTCAACATCGCCGACCGCACGGAGGCGCGGTTCGGGAAGCCGTCACGATTGG
CTTCCGGTCTCTGGAAAGTGCTGGGTCACTGA

Upstream 100 bases:

>100_bases
GGCAACCAGCGGTGAGATCTTCGGACGACCGAGGTCGGTTCGCGGGACGAACATCCCTCCCAACTCCATCGAATCCAGAG
ATTCCCGAGAGAGATCACCA

Downstream 100 bases:

>100_bases
TTGTAAGTGCTAGCCACGTCCGTGGCTGGCACGGCAGTTCCGACCGGGCACGGTTCTCGTCGGCTCGACTAGGCATGTCG
CACTCGTCAACTGGAAGTTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRDQQAHAAATPSFWHEDADLDWAPKGPLDGDLDVDVAIVGGGYTGLWTAYYLAKADPSLRIAILEKYFVGFGASGRNGG
WCSAIFPTTLRKVAADSSRSAAVRMQEAMFETVKEVGEVVVAEGIECDFQPGGYVSVARNEAQWVRSQREVDGWRAWGFG
EEHFRMLTPDEAAERVATSHTLGGTFTPHCAVVQPAKLVRGLGDVVRALGVRIFEATEVREIHSGGLSTARGTVRADVVV
RATEGYTAQLKGARREVVPMYSLMVATAPLPEEIWQEIGLRERETFSDKRHLRIYGQRTRDGRIAFGGRGAPYHFGSRVK
PEFDDDRRVHEMLRNILVDLLPTIRGVEFTHAWGGNLGIPRDWYPSVGYDRSSGIGHAGGYVGDGVATANLAGRTLAAEI
LDLENDVRSLPWVGRTTPRWEPEPLRWIGVNAGTAAFNIADRTEARFGKPSRLASGLWKVLGH

Sequences:

>Translated_463_residues
MRDQQAHAAATPSFWHEDADLDWAPKGPLDGDLDVDVAIVGGGYTGLWTAYYLAKADPSLRIAILEKYFVGFGASGRNGG
WCSAIFPTTLRKVAADSSRSAAVRMQEAMFETVKEVGEVVVAEGIECDFQPGGYVSVARNEAQWVRSQREVDGWRAWGFG
EEHFRMLTPDEAAERVATSHTLGGTFTPHCAVVQPAKLVRGLGDVVRALGVRIFEATEVREIHSGGLSTARGTVRADVVV
RATEGYTAQLKGARREVVPMYSLMVATAPLPEEIWQEIGLRERETFSDKRHLRIYGQRTRDGRIAFGGRGAPYHFGSRVK
PEFDDDRRVHEMLRNILVDLLPTIRGVEFTHAWGGNLGIPRDWYPSVGYDRSSGIGHAGGYVGDGVATANLAGRTLAAEI
LDLENDVRSLPWVGRTTPRWEPEPLRWIGVNAGTAAFNIADRTEARFGKPSRLASGLWKVLGH
>Mature_463_residues
MRDQQAHAAATPSFWHEDADLDWAPKGPLDGDLDVDVAIVGGGYTGLWTAYYLAKADPSLRIAILEKYFVGFGASGRNGG
WCSAIFPTTLRKVAADSSRSAAVRMQEAMFETVKEVGEVVVAEGIECDFQPGGYVSVARNEAQWVRSQREVDGWRAWGFG
EEHFRMLTPDEAAERVATSHTLGGTFTPHCAVVQPAKLVRGLGDVVRALGVRIFEATEVREIHSGGLSTARGTVRADVVV
RATEGYTAQLKGARREVVPMYSLMVATAPLPEEIWQEIGLRERETFSDKRHLRIYGQRTRDGRIAFGGRGAPYHFGSRVK
PEFDDDRRVHEMLRNILVDLLPTIRGVEFTHAWGGNLGIPRDWYPSVGYDRSSGIGHAGGYVGDGVATANLAGRTLAAEI
LDLENDVRSLPWVGRTTPRWEPEPLRWIGVNAGTAAFNIADRTEARFGKPSRLASGLWKVLGH

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=444, Percent_Identity=27.7027027027027, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50854; Mature: 50854

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDQQAHAAATPSFWHEDADLDWAPKGPLDGDLDVDVAIVGGGYTGLWTAYYLAKADPSL
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCE
RIAILEKYFVGFGASGRNGGWCSAIFPTTLRKVAADSSRSAAVRMQEAMFETVKEVGEVV
EEEEEHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VAEGIECDFQPGGYVSVARNEAQWVRSQREVDGWRAWGFGEEHFRMLTPDEAAERVATSH
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCCCHHHCEECCCHHHHHHHHHHC
TLGGTFTPHCAVVQPAKLVRGLGDVVRALGVRIFEATEVREIHSGGLSTARGTVRADVVV
CCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEE
RATEGYTAQLKGARREVVPMYSLMVATAPLPEEIWQEIGLRERETFSDKRHLRIYGQRTR
EECCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCEEEEEECCCC
DGRIAFGGRGAPYHFGSRVKPEFDDDRRVHEMLRNILVDLLPTIRGVEFTHAWGGNLGIP
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
RDWYPSVGYDRSSGIGHAGGYVGDGVATANLAGRTLAAEILDLENDVRSLPWVGRTTPRW
CCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPEPLRWIGVNAGTAAFNIADRTEARFGKPSRLASGLWKVLGH
CCCCCEEEECCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDQQAHAAATPSFWHEDADLDWAPKGPLDGDLDVDVAIVGGGYTGLWTAYYLAKADPSL
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCE
RIAILEKYFVGFGASGRNGGWCSAIFPTTLRKVAADSSRSAAVRMQEAMFETVKEVGEVV
EEEEEHHHHHHCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VAEGIECDFQPGGYVSVARNEAQWVRSQREVDGWRAWGFGEEHFRMLTPDEAAERVATSH
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCCCHHHCEECCCHHHHHHHHHHC
TLGGTFTPHCAVVQPAKLVRGLGDVVRALGVRIFEATEVREIHSGGLSTARGTVRADVVV
CCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEE
RATEGYTAQLKGARREVVPMYSLMVATAPLPEEIWQEIGLRERETFSDKRHLRIYGQRTR
EECCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCEEEEEECCCC
DGRIAFGGRGAPYHFGSRVKPEFDDDRRVHEMLRNILVDLLPTIRGVEFTHAWGGNLGIP
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
RDWYPSVGYDRSSGIGHAGGYVGDGVATANLAGRTLAAEILDLENDVRSLPWVGRTTPRW
CCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPEPLRWIGVNAGTAAFNIADRTEARFGKPSRLASGLWKVLGH
CCCCCEEEECCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]