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The map label for this gene is yafA [C]

Identifier: 119714869

GI number: 119714869

Start: 650063

End: 651214

Strand: Direct

Name: yafA [C]

Synonym: Noca_0613

Alternate gene names: 119714869

Gene position: 650063-651214 (Clockwise)

Preceding gene: 119714860

Following gene: 119714870

Centisome position: 13.04

GC content: 64.76

Gene sequence:

>1152_bases
ATGCCTCGGATTCGCGGAACATGTAGACGGCCGACGGCTGCGCAGTCGGGAAGATGCTGCCTCCCGACCTATGGAGAAGA
AATGACGACCGAGACCCCACAAGTCCCGACGGCCGAAGAAGAGATGCTGAACTGGGGCCGCCTGTTGATGGACGGTCTGC
CGTTTGCCGACCTCCTGGCCGCACAGCGTCGGGACGCTGAGACGTCATGGTTCGACTTCTGGATGGCGAAGGCGGACGCG
TACGAGGCCATGGGTGAGACCGCCCTGAAGGACGGCAACGACCTCACGGCCGGCTACTGGTTCTGGGTCGGATCGATGGC
CGCGCAGTACGCGCAGTTCCTGTGGTTCGACGAACGCCGCCCGAAGGGCCAGCTCCGCAAGGCAGGGCTCTATCACCGCG
CCGCTCCACTGCTCGACCCCCCGGCTGAGCGTGTGGACCTCCCTATCGACGACACCGTGATCATCGGCTACCTGCGCCGG
CCCAAGGGGGCCGACGGGCCCGTCCCGTGCGCAGTGCTGCTCGGCGGCCTCGAGAGCACGAAGGAGGAGAGCTACATGTT
CGAGAACCTCCTACTCGAGCGAGGCGTCGCCACCTTCACGTTCGACGGTCCCGGCCAGGGTGAGATGTTCGAGGACGTCG
CACTCGCCGGCGACTACCACCGCTACACCTCACGGGTGGTCGACTACCTCGAGACCCTAGGGGTCACTATCGACGAGGAT
CGGATCGGAGTGCTGGGCCGCAGCCTGGGAGGACATTATGCGCTGCGTGCCGCCTCAATGGACGACCGCTTCCGTGCATG
CGTCACCTGGGGTGGTTTCGTCCAGATGGACGACTGGGATTTCGAGTACCCCATGACCAAGCTCAGCTGGCAGTACGTAA
CGAAGTCGCCCGATCTGCCCACTGCGCAGGAGCGGGTGAAGGAAGCCATCGACGTACGCCCCGTGCTGGCTGGACTGAAG
GTACCCACGTACGTGATGCACGGGGCCAAGGACGAGACTCCTCTCACCGAGCTGGATCTGTTGCAGGAGTACGCGGTCAA
CGCGGACATCACGATTGATCTCGAGCCCGAGGGCGATCACTGCTGCCACAACCTCGGTCCCGCCCCGCGCCTTCGCATGG
CGGACTGGCTGGCGAACCAGCTTCGCCCCTGA

Upstream 100 bases:

>100_bases
GGCGCCGTTGCTGCCGCTGCTCGCTGGCGGGAGAAGCGCATCCGCCCGATGGGCAGATCATGTACATATGCCTTCCCATT
GCTAGGTGGGCGCCAAAGAC

Downstream 100 bases:

>100_bases
ACCGGCTCCGCGCGGCGTTCGCGACCGCCGCGTCGCGCGGGCCACACCACACCCCTCAGAGCAGCCGAGTAGCTGCAGAA
CGGTAGGAACTTCATGGATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPRIRGTCRRPTAAQSGRCCLPTYGEEMTTETPQVPTAEEEMLNWGRLLMDGLPFADLLAAQRRDAETSWFDFWMAKADA
YEAMGETALKDGNDLTAGYWFWVGSMAAQYAQFLWFDERRPKGQLRKAGLYHRAAPLLDPPAERVDLPIDDTVIIGYLRR
PKGADGPVPCAVLLGGLESTKEESYMFENLLLERGVATFTFDGPGQGEMFEDVALAGDYHRYTSRVVDYLETLGVTIDED
RIGVLGRSLGGHYALRAASMDDRFRACVTWGGFVQMDDWDFEYPMTKLSWQYVTKSPDLPTAQERVKEAIDVRPVLAGLK
VPTYVMHGAKDETPLTELDLLQEYAVNADITIDLEPEGDHCCHNLGPAPRLRMADWLANQLRP

Sequences:

>Translated_383_residues
MPRIRGTCRRPTAAQSGRCCLPTYGEEMTTETPQVPTAEEEMLNWGRLLMDGLPFADLLAAQRRDAETSWFDFWMAKADA
YEAMGETALKDGNDLTAGYWFWVGSMAAQYAQFLWFDERRPKGQLRKAGLYHRAAPLLDPPAERVDLPIDDTVIIGYLRR
PKGADGPVPCAVLLGGLESTKEESYMFENLLLERGVATFTFDGPGQGEMFEDVALAGDYHRYTSRVVDYLETLGVTIDED
RIGVLGRSLGGHYALRAASMDDRFRACVTWGGFVQMDDWDFEYPMTKLSWQYVTKSPDLPTAQERVKEAIDVRPVLAGLK
VPTYVMHGAKDETPLTELDLLQEYAVNADITIDLEPEGDHCCHNLGPAPRLRMADWLANQLRP
>Mature_382_residues
PRIRGTCRRPTAAQSGRCCLPTYGEEMTTETPQVPTAEEEMLNWGRLLMDGLPFADLLAAQRRDAETSWFDFWMAKADAY
EAMGETALKDGNDLTAGYWFWVGSMAAQYAQFLWFDERRPKGQLRKAGLYHRAAPLLDPPAERVDLPIDDTVIIGYLRRP
KGADGPVPCAVLLGGLESTKEESYMFENLLLERGVATFTFDGPGQGEMFEDVALAGDYHRYTSRVVDYLETLGVTIDEDR
IGVLGRSLGGHYALRAASMDDRFRACVTWGGFVQMDDWDFEYPMTKLSWQYVTKSPDLPTAQERVKEAIDVRPVLAGLKV
PTYVMHGAKDETPLTELDLLQEYAVNADITIDLEPEGDHCCHNLGPAPRLRMADWLANQLRP

Specific function: L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6- dihydroxypseudooxyicotine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0255 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010520 [H]

Pfam domain/function: PF06500 DUF1100 [H]

EC number: NA

Molecular weight: Translated: 43019; Mature: 42888

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIRGTCRRPTAAQSGRCCLPTYGEEMTTETPQVPTAEEEMLNWGRLLMDGLPFADLLA
CCCCCCCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
AQRRDAETSWFDFWMAKADAYEAMGETALKDGNDLTAGYWFWVGSMAAQYAQFLWFDERR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PKGQLRKAGLYHRAAPLLDPPAERVDLPIDDTVIIGYLRRPKGADGPVPCAVLLGGLEST
CHHHHHHHCHHHHCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCHHHH
KEESYMFENLLLERGVATFTFDGPGQGEMFEDVALAGDYHRYTSRVVDYLETLGVTIDED
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCEECCC
RIGVLGRSLGGHYALRAASMDDRFRACVTWGGFVQMDDWDFEYPMTKLSWQYVTKSPDLP
CHHHHHHCCCCCEEEEECCCCHHHHHHEEECCEEEECCCCCCCCHHHHEEEEEECCCCCC
TAQERVKEAIDVRPVLAGLKVPTYVMHGAKDETPLTELDLLQEYAVNADITIDLEPEGDH
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
CCHNLGPAPRLRMADWLANQLRP
HHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PRIRGTCRRPTAAQSGRCCLPTYGEEMTTETPQVPTAEEEMLNWGRLLMDGLPFADLLA
CCCCCCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
AQRRDAETSWFDFWMAKADAYEAMGETALKDGNDLTAGYWFWVGSMAAQYAQFLWFDERR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PKGQLRKAGLYHRAAPLLDPPAERVDLPIDDTVIIGYLRRPKGADGPVPCAVLLGGLEST
CHHHHHHHCHHHHCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCHHHH
KEESYMFENLLLERGVATFTFDGPGQGEMFEDVALAGDYHRYTSRVVDYLETLGVTIDED
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCEECCC
RIGVLGRSLGGHYALRAASMDDRFRACVTWGGFVQMDDWDFEYPMTKLSWQYVTKSPDLP
CHHHHHHCCCCCEEEEECCCCHHHHHHEEECCEEEECCCCCCCCHHHHEEEEEECCCCCC
TAQERVKEAIDVRPVLAGLKVPTYVMHGAKDETPLTELDLLQEYAVNADITIDLEPEGDH
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
CCHNLGPAPRLRMADWLANQLRP
HHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11514508; 12618462 [H]