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The map label for this gene is cysU [H]

Identifier: 119714803

GI number: 119714803

Start: 573958

End: 574839

Strand: Direct

Name: cysU [H]

Synonym: Noca_0539

Alternate gene names: 119714803

Gene position: 573958-574839 (Clockwise)

Preceding gene: 119714802

Following gene: 119714804

Centisome position: 11.51

GC content: 71.66

Gene sequence:

>882_bases
ATGCCCACTGCCCTCCTGGAGGGGCCGCGCGACAGCGCCGAGCCGGCGTCCCATGGGGGACGCCGGCGTCGGCGCGCCCG
TCGTACGGCTCGGCGCAACACCTTGACCCCCGGCGCCGGTCTCGGGCTCGGGATCTCCATGATCTGGTTCAGCCTGCTGG
TGCTGATCCCGCTCGCCGCCGTGGTCGCGGCGGCGTCCGCGGGTGGCTGGGACGGCTACCTCCGGGTGCTGCAGAACGAC
CAGACCTGGGCGGCGATCAAGCTGACGGTCACCCAGTCCCTGGCCGTGACCCTGGTCAACGTCGTGATGGGCACGGTCAT
CGCCTGGGTGCTGGTCCGGGACCGGTTCTGGGGCCGTTCGGTGCTGGACGTCATCATCGACGTGCCGTTCGCGATGCCGA
CAGTCGTCGCCGGCCTGCTCCTGCTCTCGTTGTACGGCCCGAGCAGCCCGATCGGGGTGCACTGGGCCAACACCGAGCGG
GCGGTGAGCCTCGCGCTCGCGTTCGTCACCCTGCCGTTCGTCGTCCGGACCGTGCAGCCGGTGCTCGAGGAGCTCGACGC
CGACGTCGAGGAGGCTGCCGCCTCCCTCGGCGCAGGTCGCGCGACGGTCGTCCGCAGGATCGTGCTGCCGAGCCTCGCCC
CCGCGATCGCGGCCGGCGCGGCCCTGTCGTTCGCGCGCGCCATCTCGGAGTACGGGTCCCTCGTGCTGCTCAGCGGCAAC
CGTCCGTTCCAGACCGAGGTCGTGTCGGTGCGGGTGCTCACGTTCATCGAGAACGGCAGCAACACCTCGGCGGCCGCGGT
CGCCTCGGTGATGCTGGCCGTCGCGTTGGTCGTGATCGTCTCCCTCGACCTCGTCCAGCGAAGGGTGGCCCGCCGTGGTT
GA

Upstream 100 bases:

>100_bases
GTGAGGCAGCCGACAAGTACTTCGGTGACGGCGAGGAGGGGCACCCCCTCGGCATCATCACCGAGCTGCAGCAGCAGACC
GGCAAGGTGGGCGAGGAGTA

Downstream 100 bases:

>100_bases
GGTCTCCACGCCCACCCGTTGGCTGCTGCGGGCGCTCGCCACCGGCTACGTGTTCTTCCTCGTGGCCTGGCCGGTCGGCC
TGCTGGGACGCCGTACGTTC

Product: sulfate ABC transporter, inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPTALLEGPRDSAEPASHGGRRRRRARRTARRNTLTPGAGLGLGISMIWFSLLVLIPLAAVVAAASAGGWDGYLRVLQND
QTWAAIKLTVTQSLAVTLVNVVMGTVIAWVLVRDRFWGRSVLDVIIDVPFAMPTVVAGLLLLSLYGPSSPIGVHWANTER
AVSLALAFVTLPFVVRTVQPVLEELDADVEEAAASLGAGRATVVRRIVLPSLAPAIAAGAALSFARAISEYGSLVLLSGN
RPFQTEVVSVRVLTFIENGSNTSAAAVASVMLAVALVVIVSLDLVQRRVARRG

Sequences:

>Translated_293_residues
MPTALLEGPRDSAEPASHGGRRRRRARRTARRNTLTPGAGLGLGISMIWFSLLVLIPLAAVVAAASAGGWDGYLRVLQND
QTWAAIKLTVTQSLAVTLVNVVMGTVIAWVLVRDRFWGRSVLDVIIDVPFAMPTVVAGLLLLSLYGPSSPIGVHWANTER
AVSLALAFVTLPFVVRTVQPVLEELDADVEEAAASLGAGRATVVRRIVLPSLAPAIAAGAALSFARAISEYGSLVLLSGN
RPFQTEVVSVRVLTFIENGSNTSAAAVASVMLAVALVVIVSLDLVQRRVARRG
>Mature_292_residues
PTALLEGPRDSAEPASHGGRRRRRARRTARRNTLTPGAGLGLGISMIWFSLLVLIPLAAVVAAASAGGWDGYLRVLQNDQ
TWAAIKLTVTQSLAVTLVNVVMGTVIAWVLVRDRFWGRSVLDVIIDVPFAMPTVVAGLLLLSLYGPSSPIGVHWANTERA
VSLALAFVTLPFVVRTVQPVLEELDADVEEAAASLGAGRATVVRRIVLPSLAPAIAAGAALSFARAISEYGSLVLLSGNR
PFQTEVVSVRVLTFIENGSNTSAAAVASVMLAVALVVIVSLDLVQRRVARRG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=41.2878787878788, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082099, Length=264, Percent_Identity=31.4393939393939, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=31.6742081447964, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787368, Length=227, Percent_Identity=26.431718061674, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787081, Length=230, Percent_Identity=30.8695652173913, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30958; Mature: 30826

Theoretical pI: Translated: 11.65; Mature: 11.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTALLEGPRDSAEPASHGGRRRRRARRTARRNTLTPGAGLGLGISMIWFSLLVLIPLAA
CCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVAAASAGGWDGYLRVLQNDQTWAAIKLTVTQSLAVTLVNVVMGTVIAWVLVRDRFWGRS
HHHHHCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLDVIIDVPFAMPTVVAGLLLLSLYGPSSPIGVHWANTERAVSLALAFVTLPFVVRTVQP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VLEELDADVEEAAASLGAGRATVVRRIVLPSLAPAIAAGAALSFARAISEYGSLVLLSGN
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
RPFQTEVVSVRVLTFIENGSNTSAAAVASVMLAVALVVIVSLDLVQRRVARRG
CCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTALLEGPRDSAEPASHGGRRRRRARRTARRNTLTPGAGLGLGISMIWFSLLVLIPLAA
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVAAASAGGWDGYLRVLQNDQTWAAIKLTVTQSLAVTLVNVVMGTVIAWVLVRDRFWGRS
HHHHHCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLDVIIDVPFAMPTVVAGLLLLSLYGPSSPIGVHWANTERAVSLALAFVTLPFVVRTVQP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VLEELDADVEEAAASLGAGRATVVRRIVLPSLAPAIAAGAALSFARAISEYGSLVLLSGN
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
RPFQTEVVSVRVLTFIENGSNTSAAAVASVMLAVALVVIVSLDLVQRRVARRG
CCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]