BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is hdhA [H]

Identifier: 119714691

GI number: 119714691

Start: 457322

End: 458134

Strand: Direct

Name: hdhA [H]

Synonym: Noca_0427

Alternate gene names: 119714691

Gene position: 457322-458134 (Clockwise)

Preceding gene: 119714690

Following gene: 119714692

Centisome position: 9.17

GC content: 72.32

Gene sequence:

>813_bases
ATGATCCTCGACCGCTTCTCGACCGAGGGCCAGGTCGCGATCGTCACCGGCGCCGGCCGCGGCCTCGGCGCCGCCACCGC
CGTCGCGCTCGCCGAGGCCGGCTCGGACGTGCTGATCTCCGCCCGCACCGAGCGCCAGCTCGACCAGGTCGCCGAGCGGA
TCCGGGCCACCGGCCGGCGCGCGCTCGTGGTCGCCGCGGACCTCAGCGACCTCGACGCGGTCGCCGGCCTCGCGCAGACG
GCGTACGACGAGCTCGGCCGGCTCGACACGGTCGTGAACAACGTCGGCGGCACGTTCCCGAACGCGTTCCTCGACACCAG
CAACGAGTTCCTCAACGAGGCGTTCGCCTTCAACGTCACCACCGCGCATGCGCTCACCCGCGCGGCGGTGCCGCTGATGC
TGAGGGACGCCGGATCGGTGGCGGACGCGCAGAAGAGCGTCGTCACGATCAGCTCGATGATGGGCCGCACCGCCGATCGC
GGCTTCATCGCCTACGGCACCGCCAAGGCCGCGCTCGCGCACTGGACGAGGATGGCGGCCCAGGACCTCGCGCCGCGGAT
CCGCGTCAACGGCATCTACGTCGGGTCGATCATGACCAGCGCGCTGGAGTACGTCGCCGGCCGGCCGGAGCTGATGAGCC
AGCTCGAGACGAAGACCCCGCTCGGGCGCGTCGGCGAGCCCGAGGACATCGCGGCCGGCGTCGTCTACCTCGCCTCGCGG
GCCGGGCAGTATGTGACCGGCAAGCTGCTCGAGATCGACGGCGGCATCCAGCAGCCGACCCTCGACCTCGGGTTCCCCGA
CGTCGGGTCGTAG

Upstream 100 bases:

>100_bases
GACCTCCCGCTCGTGACCGGTACGGGCCTGGTGAGCCGCGCATGAGCCACTTCGCCCCCCGAGATCGCTCGCTGCGCTCC
CGATTCCGCGGAGGCCCCGA

Downstream 100 bases:

>100_bases
GGTCCGTGCGTGACGCCATCCACCTCCCCAGCCTCCACGCCGACGGCGTTCGCCGTCTCGCCCGACTCCGGCGAGCACTG
GAGCAAGGCGGGAGCCTGGC

Product: short chain dehydrogenase

Products: NA

Alternate protein names: 7-alpha-HSDH [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MILDRFSTEGQVAIVTGAGRGLGAATAVALAEAGSDVLISARTERQLDQVAERIRATGRRALVVAADLSDLDAVAGLAQT
AYDELGRLDTVVNNVGGTFPNAFLDTSNEFLNEAFAFNVTTAHALTRAAVPLMLRDAGSVADAQKSVVTISSMMGRTADR
GFIAYGTAKAALAHWTRMAAQDLAPRIRVNGIYVGSIMTSALEYVAGRPELMSQLETKTPLGRVGEPEDIAAGVVYLASR
AGQYVTGKLLEIDGGIQQPTLDLGFPDVGS

Sequences:

>Translated_270_residues
MILDRFSTEGQVAIVTGAGRGLGAATAVALAEAGSDVLISARTERQLDQVAERIRATGRRALVVAADLSDLDAVAGLAQT
AYDELGRLDTVVNNVGGTFPNAFLDTSNEFLNEAFAFNVTTAHALTRAAVPLMLRDAGSVADAQKSVVTISSMMGRTADR
GFIAYGTAKAALAHWTRMAAQDLAPRIRVNGIYVGSIMTSALEYVAGRPELMSQLETKTPLGRVGEPEDIAAGVVYLASR
AGQYVTGKLLEIDGGIQQPTLDLGFPDVGS
>Mature_270_residues
MILDRFSTEGQVAIVTGAGRGLGAATAVALAEAGSDVLISARTERQLDQVAERIRATGRRALVVAADLSDLDAVAGLAQT
AYDELGRLDTVVNNVGGTFPNAFLDTSNEFLNEAFAFNVTTAHALTRAAVPLMLRDAGSVADAQKSVVTISSMMGRTADR
GFIAYGTAKAALAHWTRMAAQDLAPRIRVNGIYVGSIMTSALEYVAGRPELMSQLETKTPLGRVGEPEDIAAGVVYLASR
AGQYVTGKLLEIDGGIQQPTLDLGFPDVGS

Specific function: 7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI10190704, Length=278, Percent_Identity=32.7338129496403, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI19923817, Length=270, Percent_Identity=27.037037037037, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI59889578, Length=243, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI126723750, Length=250, Percent_Identity=28, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=27.0916334661355, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI210032110, Length=168, Percent_Identity=27.3809523809524, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI7706318, Length=198, Percent_Identity=28.2828282828283, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI66933014, Length=253, Percent_Identity=28.4584980237154, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI142976729, Length=229, Percent_Identity=23.1441048034935, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38348362, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=28.3422459893048, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40254986, Length=216, Percent_Identity=25.462962962963, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI38424085, Length=172, Percent_Identity=31.3953488372093, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=33.9920948616601, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=30.46875, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1789378, Length=249, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=30.46875, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=30.5555555555556, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=30.5660377358491, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=26.2745098039216, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=31.6, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=30, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=30.3501945525292, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=29.718875502008, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=32.5925925925926, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994600, Length=260, Percent_Identity=29.6153846153846, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=30.8880308880309, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI72000259, Length=271, Percent_Identity=30.6273062730627, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=31.7343173431734, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=31.4176245210728, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560332, Length=272, Percent_Identity=32.3529411764706, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17562904, Length=271, Percent_Identity=28.7822878228782, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531453, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560150, Length=267, Percent_Identity=29.2134831460674, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17562910, Length=281, Percent_Identity=28.8256227758007, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=30.241935483871, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562908, Length=282, Percent_Identity=31.5602836879433, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17551412, Length=268, Percent_Identity=29.4776119402985, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=267, Percent_Identity=29.5880149812734, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538486, Length=264, Percent_Identity=30.3030303030303, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=29.1044776119403, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538480, Length=278, Percent_Identity=29.8561151079137, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=256, Percent_Identity=30.46875, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=29.368029739777, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17563726, Length=269, Percent_Identity=28.996282527881, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=268, Percent_Identity=27.6119402985075, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=25.1851851851852, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17508651, Length=258, Percent_Identity=28.6821705426357, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=275, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=189, Percent_Identity=32.8042328042328, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=187, Percent_Identity=26.2032085561497, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=158, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=26.2096774193548, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997099, Length=195, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=167, Percent_Identity=25.748502994012, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=33.984375, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI23397609, Length=261, Percent_Identity=31.4176245210728, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=32.421875, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=30.3149606299213, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=28.4584980237154, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=29.3650793650794, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644337, Length=257, Percent_Identity=30.7392996108949, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=30.241935483871, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580925, Length=138, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.159 [H]

Molecular weight: Translated: 28257; Mature: 28257

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDRFSTEGQVAIVTGAGRGLGAATAVALAEAGSDVLISARTERQLDQVAERIRATGRR
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCE
ALVVAADLSDLDAVAGLAQTAYDELGRLDTVVNNVGGTFPNAFLDTSNEFLNEAFAFNVT
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCH
TAHALTRAAVPLMLRDAGSVADAQKSVVTISSMMGRTADRGFIAYGTAKAALAHWTRMAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
QDLAPRIRVNGIYVGSIMTSALEYVAGRPELMSQLETKTPLGRVGEPEDIAAGVVYLASR
HHCCCEEEECCEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AGQYVTGKLLEIDGGIQQPTLDLGFPDVGS
CCCEEEEEEEEECCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MILDRFSTEGQVAIVTGAGRGLGAATAVALAEAGSDVLISARTERQLDQVAERIRATGRR
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCE
ALVVAADLSDLDAVAGLAQTAYDELGRLDTVVNNVGGTFPNAFLDTSNEFLNEAFAFNVT
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCH
TAHALTRAAVPLMLRDAGSVADAQKSVVTISSMMGRTADRGFIAYGTAKAALAHWTRMAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
QDLAPRIRVNGIYVGSIMTSALEYVAGRPELMSQLETKTPLGRVGEPEDIAAGVVYLASR
HHCCCEEEECCEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AGQYVTGKLLEIDGGIQQPTLDLGFPDVGS
CCCEEEEEEEEECCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]