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The map label for this gene is ask [H]

Identifier: 119714587

GI number: 119714587

Start: 343857

End: 345131

Strand: Direct

Name: ask [H]

Synonym: Noca_0321

Alternate gene names: 119714587

Gene position: 343857-345131 (Clockwise)

Preceding gene: 119714586

Following gene: 119714588

Centisome position: 6.9

GC content: 69.96

Gene sequence:

>1275_bases
GTGGGCATTGTCGTCCAGAAGTACGGCGGATCGTCGGTCGCGGACGCCGCCGGCATCAAGCGGGTGGCCCAGCGGATCGT
CAACACCCGCAAGGCCGGCCACGACGTCGTCGTGGTCGTCTCGGCCATGGGAGACGAGACCGACAACCTGCGCGATCTCG
CCGAGCAGGTGACGCCGCTGCCGCCGCCGCGGGAGCTGGACATGCTGCTGACCGCCGGCGAGCGGATCTCGATGGCGCTG
GTCGCGATGGCCATCGCCGCGCTCGGGCACGAGGCGAAGTCGTTCACCGGCTCGCAGGCGGGCGTGATCACCGACTCCGC
CCACGGCAAGGCGAAGATCATCGACATCACGCCGGGCCGGATCGAGAAGGCCCTCAAGGACGGCGCCATCGCGATCGTCG
CCGGCTTCCAGGGCGTCTCCCAGGACACCAAGGACGTCACCACGCTCGGCCGCGGCGCCTCGGACACGACCGCGGTCGCG
CTCGCCGCCGCGCTCGGTGCCGAGGTCTGCGAGATCTACAGCGACGTCGACGGCGTCTTCACCGCGGACCCCCGGATCGT
GCCCGCGGCCCGCAAGCTCGCCCGGGTCTCGACCGAGGAGATGCTGGAGATGGCGGCCTCGGGCGCCAAGATCCTGCACC
TGCGGTGCGTCGAGTACGCCCGCCGTTACGACATGCCGATCCATGTGCGCTCCTCCTTCAGCCAGAAGGAAGGCACCTGG
GTCATCCCGGACAGCAAGGCAGGAGAGGACGAGATGGAGCAGGCGATCATCGCCGGCGTCGCCCACGACCGGAGCGAGGC
CAAGATCACCGTGGTCGGCGTGCCGGACAAGGTCGGCGAGGCGGCCCGGATCTTCGAGGCGCTGGCCGCGACCGAGGTCA
ACCTCGACATGGTCGTCCAGAACGTCTCCGCGGCGGCCACCGGCCTGACCGACATCTCCTTCACGCTGCCGCGCGCGGAC
GGGCAGAGCGCGATGGGCGCCCTGGCGCGGATCCAGGACGAGGTCGGCTACGACAAGCTGCTCTACGACGACCAGATCGG
CAAGGTCTCGCTGATCGGGGCGGGCATGCGCTCCCACCCCGGCATCACCGCGCGCTTCTTCGCCTCACTCGCCGCGGCGG
GCGTCAACATCGAGATGATCTCCACCTCCGAGATCCGGATCTCGGTGATCGTCGACGAGGCCCAGGTCGACGAGGCGGTG
CGCGCCACGCACACCGCGTTCGACCTCGACGCCGAAGAGGTCGAGGCGGTCGTGTACGGCGGGACCGGTCGATGA

Upstream 100 bases:

>100_bases
GCGCTCGCCGATGAGATGTTGGAGGGTGGCGAGCCCACCGCTCGGTAGACTCGACGGCTGGCAGCACACCGAGCCAGCAG
TCGAGCAGTAGGGACCAACC

Downstream 100 bases:

>100_bases
GCGCCCGCCGCATCGACGTCGGCGTCGTTGGTGCTACCGGACAGCACCCCCCGCGGAGTTCCTCGCTCCTCCGCTGCGCT
CCCCCGCGAACAACTCCGCG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGIVVQKYGGSSVADAAGIKRVAQRIVNTRKAGHDVVVVVSAMGDETDNLRDLAEQVTPLPPPRELDMLLTAGERISMAL
VAMAIAALGHEAKSFTGSQAGVITDSAHGKAKIIDITPGRIEKALKDGAIAIVAGFQGVSQDTKDVTTLGRGASDTTAVA
LAAALGAEVCEIYSDVDGVFTADPRIVPAARKLARVSTEEMLEMAASGAKILHLRCVEYARRYDMPIHVRSSFSQKEGTW
VIPDSKAGEDEMEQAIIAGVAHDRSEAKITVVGVPDKVGEAARIFEALAATEVNLDMVVQNVSAAATGLTDISFTLPRAD
GQSAMGALARIQDEVGYDKLLYDDQIGKVSLIGAGMRSHPGITARFFASLAAAGVNIEMISTSEIRISVIVDEAQVDEAV
RATHTAFDLDAEEVEAVVYGGTGR

Sequences:

>Translated_424_residues
MGIVVQKYGGSSVADAAGIKRVAQRIVNTRKAGHDVVVVVSAMGDETDNLRDLAEQVTPLPPPRELDMLLTAGERISMAL
VAMAIAALGHEAKSFTGSQAGVITDSAHGKAKIIDITPGRIEKALKDGAIAIVAGFQGVSQDTKDVTTLGRGASDTTAVA
LAAALGAEVCEIYSDVDGVFTADPRIVPAARKLARVSTEEMLEMAASGAKILHLRCVEYARRYDMPIHVRSSFSQKEGTW
VIPDSKAGEDEMEQAIIAGVAHDRSEAKITVVGVPDKVGEAARIFEALAATEVNLDMVVQNVSAAATGLTDISFTLPRAD
GQSAMGALARIQDEVGYDKLLYDDQIGKVSLIGAGMRSHPGITARFFASLAAAGVNIEMISTSEIRISVIVDEAQVDEAV
RATHTAFDLDAEEVEAVVYGGTGR
>Mature_423_residues
GIVVQKYGGSSVADAAGIKRVAQRIVNTRKAGHDVVVVVSAMGDETDNLRDLAEQVTPLPPPRELDMLLTAGERISMALV
AMAIAALGHEAKSFTGSQAGVITDSAHGKAKIIDITPGRIEKALKDGAIAIVAGFQGVSQDTKDVTTLGRGASDTTAVAL
AAALGAEVCEIYSDVDGVFTADPRIVPAARKLARVSTEEMLEMAASGAKILHLRCVEYARRYDMPIHVRSSFSQKEGTWV
IPDSKAGEDEMEQAIIAGVAHDRSEAKITVVGVPDKVGEAARIFEALAATEVNLDMVVQNVSAAATGLTDISFTLPRADG
QSAMGALARIQDEVGYDKLLYDDQIGKVSLIGAGMRSHPGITARFFASLAAAGVNIEMISTSEIRISVIVDEAQVDEAVR
ATHTAFDLDAEEVEAVVYGGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=355, Percent_Identity=31.830985915493, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=26.7391304347826, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790376, Length=482, Percent_Identity=23.4439834024896, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=373, Percent_Identity=26.2734584450402, Blast_Score=109, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44527; Mature: 44396

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIVVQKYGGSSVADAAGIKRVAQRIVNTRKAGHDVVVVVSAMGDETDNLRDLAEQVTPL
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
PPPRELDMLLTAGERISMALVAMAIAALGHEAKSFTGSQAGVITDSAHGKAKIIDITPGR
CCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCCCCEEEEECCCHH
IEKALKDGAIAIVAGFQGVSQDTKDVTTLGRGASDTTAVALAAALGAEVCEIYSDVDGVF
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
TADPRIVPAARKLARVSTEEMLEMAASGAKILHLRCVEYARRYDMPIHVRSSFSQKEGTW
ECCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCE
VIPDSKAGEDEMEQAIIAGVAHDRSEAKITVVGVPDKVGEAARIFEALAATEVNLDMVVQ
EECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHH
NVSAAATGLTDISFTLPRADGQSAMGALARIQDEVGYDKLLYDDQIGKVSLIGAGMRSHP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCC
GITARFFASLAAAGVNIEMISTSEIRISVIVDEAQVDEAVRATHTAFDLDAEEVEAVVYG
CHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEEEC
GTGR
CCCC
>Mature Secondary Structure 
GIVVQKYGGSSVADAAGIKRVAQRIVNTRKAGHDVVVVVSAMGDETDNLRDLAEQVTPL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
PPPRELDMLLTAGERISMALVAMAIAALGHEAKSFTGSQAGVITDSAHGKAKIIDITPGR
CCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCCCCEEEEECCCHH
IEKALKDGAIAIVAGFQGVSQDTKDVTTLGRGASDTTAVALAAALGAEVCEIYSDVDGVF
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
TADPRIVPAARKLARVSTEEMLEMAASGAKILHLRCVEYARRYDMPIHVRSSFSQKEGTW
ECCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCE
VIPDSKAGEDEMEQAIIAGVAHDRSEAKITVVGVPDKVGEAARIFEALAATEVNLDMVVQ
EECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHH
NVSAAATGLTDISFTLPRADGQSAMGALARIQDEVGYDKLLYDDQIGKVSLIGAGMRSHP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCC
GITARFFASLAAAGVNIEMISTSEIRISVIVDEAQVDEAVRATHTAFDLDAEEVEAVVYG
CHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEEEC
GTGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972; 7910936 [H]