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The map label for this gene is purU [H]

Identifier: 119714394

GI number: 119714394

Start: 139877

End: 140725

Strand: Reverse

Name: purU [H]

Synonym: Noca_0127

Alternate gene names: 119714394

Gene position: 140725-139877 (Counterclockwise)

Preceding gene: 119714399

Following gene: 119714385

Centisome position: 2.82

GC content: 70.32

Gene sequence:

>849_bases
GTGTCCGAGCTCGTGCTGATCCTGTCCTGTCCCGACCGTCCCGGCATCGTGCATGCCGTCACCGGGTTCCTGGTGGAGCG
CGGTGCGAACATCACCGAGAGCCAGCAGTTCGGCGACCCGCTCAGCGGCCGGTTCTTCATGCGGATCGCCGTGGACGTGC
CGGGAGCCGTCGACGCGGCGGCCCTGCGGGCGGAGTTCGCGCCGGTCGCGGAGGAGTTCGAGATGCGCTTCGACGTCCAC
GACGCGGCCGCGCCGTACCGCACGCTGATCCTGGTCTCCAAGGACCTGCACTGCCTCAACGACCTGCTGTTCCGCACCAG
CACCGGCTCGCTCGGCATCGAGGTGCCGGCGATCGTGTCGAACCACCCGGACGCCGCGGCGATGGCGGCGTCGTACGGCG
TGCCGTTCCACCACGTCCCGGTCACCCCGGACACCAAGGCGCAGGCCGAGGAGCGGCTGCTCGAGCTGGTGCGCGAGCTC
GACATCGACCTGGTGGTGCTCGCCCGCTACATGCAGATCCTCTCCGACGGGCTGTGCCGCGAGCTGTCCGGCAAGGCGAT
CAACATCCACCACTCGTTCCTGCCCAGCTTCAAGGGCGCCCGGCCCTACCACCAGGCCTTCGACCGCGGGGTCAAGCTGG
TCGGCGCGACCGCGCACTACGTGACCAGCGACCTCGACGAGGGCCCGATCATCGAGCAGGACGTGATGCGGGTCGACCAC
ACCCACCACCAGGAGCAGCTGGTCTCCGCGGGCCGCGACGTCGAGGCGCAGGTGCTGTCCCGCGCGGTGCGCTGGCACGC
GCAGTCCCGGGTGCTGCTCAACGGCCACCGCACCGTCGTGTTCCGCTGA

Upstream 100 bases:

>100_bases
CATCCCTCCCCGGGCCAGGGTACGTCGTGAGCCGGCGTCGCCGGTCCGCCCGCAGGGCCGTGCGGCCGGGCCTCCCCACC
CCGATGGAAGACTGTCCCCC

Downstream 100 bases:

>100_bases
CCCCCCGGCACATCGGTCAGGCCGTCCAGCGCACCGCCTCGGCGGCCAGGTCGGCCGCACCGTAGACGCCGGCCAGGTCG
GGACGGCGGGCCATCAGCTC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSELVLILSCPDRPGIVHAVTGFLVERGANITESQQFGDPLSGRFFMRIAVDVPGAVDAAALRAEFAPVAEEFEMRFDVH
DAAAPYRTLILVSKDLHCLNDLLFRTSTGSLGIEVPAIVSNHPDAAAMAASYGVPFHHVPVTPDTKAQAEERLLELVREL
DIDLVVLARYMQILSDGLCRELSGKAINIHHSFLPSFKGARPYHQAFDRGVKLVGATAHYVTSDLDEGPIIEQDVMRVDH
THHQEQLVSAGRDVEAQVLSRAVRWHAQSRVLLNGHRTVVFR

Sequences:

>Translated_282_residues
MSELVLILSCPDRPGIVHAVTGFLVERGANITESQQFGDPLSGRFFMRIAVDVPGAVDAAALRAEFAPVAEEFEMRFDVH
DAAAPYRTLILVSKDLHCLNDLLFRTSTGSLGIEVPAIVSNHPDAAAMAASYGVPFHHVPVTPDTKAQAEERLLELVREL
DIDLVVLARYMQILSDGLCRELSGKAINIHHSFLPSFKGARPYHQAFDRGVKLVGATAHYVTSDLDEGPIIEQDVMRVDH
THHQEQLVSAGRDVEAQVLSRAVRWHAQSRVLLNGHRTVVFR
>Mature_281_residues
SELVLILSCPDRPGIVHAVTGFLVERGANITESQQFGDPLSGRFFMRIAVDVPGAVDAAALRAEFAPVAEEFEMRFDVHD
AAAPYRTLILVSKDLHCLNDLLFRTSTGSLGIEVPAIVSNHPDAAAMAASYGVPFHHVPVTPDTKAQAEERLLELVRELD
IDLVVLARYMQILSDGLCRELSGKAINIHHSFLPSFKGARPYHQAFDRGVKLVGATAHYVTSDLDEGPIIEQDVMRVDHT
HHQEQLVSAGRDVEAQVLSRAVRWHAQSRVLLNGHRTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=40.7942238267148, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=28.2722513089005, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=157, Percent_Identity=36.9426751592357, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24582400, Length=154, Percent_Identity=32.4675324675325, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31094; Mature: 30963

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELVLILSCPDRPGIVHAVTGFLVERGANITESQQFGDPLSGRFFMRIAVDVPGAVDAA
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEEEECCCCHHHH
ALRAEFAPVAEEFEMRFDVHDAAAPYRTLILVSKDLHCLNDLLFRTSTGSLGIEVPAIVS
HHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEECHHCC
NHPDAAAMAASYGVPFHHVPVTPDTKAQAEERLLELVRELDIDLVVLARYMQILSDGLCR
CCCCHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ELSGKAINIHHSFLPSFKGARPYHQAFDRGVKLVGATAHYVTSDLDEGPIIEQDVMRVDH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHH
THHQEQLVSAGRDVEAQVLSRAVRWHAQSRVLLNGHRTVVFR
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEEC
>Mature Secondary Structure 
SELVLILSCPDRPGIVHAVTGFLVERGANITESQQFGDPLSGRFFMRIAVDVPGAVDAA
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEEEECCCCHHHH
ALRAEFAPVAEEFEMRFDVHDAAAPYRTLILVSKDLHCLNDLLFRTSTGSLGIEVPAIVS
HHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEECHHCC
NHPDAAAMAASYGVPFHHVPVTPDTKAQAEERLLELVRELDIDLVVLARYMQILSDGLCR
CCCCHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ELSGKAINIHHSFLPSFKGARPYHQAFDRGVKLVGATAHYVTSDLDEGPIIEQDVMRVDH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHH
THHQEQLVSAGRDVEAQVLSRAVRWHAQSRVLLNGHRTVVFR
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]