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The map label for this gene is alkB [H]

Identifier: 119714389

GI number: 119714389

Start: 135349

End: 136794

Strand: Direct

Name: alkB [H]

Synonym: Noca_0122

Alternate gene names: 119714389

Gene position: 135349-136794 (Clockwise)

Preceding gene: 119714388

Following gene: 119714390

Centisome position: 2.71

GC content: 70.4

Gene sequence:

>1446_bases
ATGACCGCGACCCCCCCGACCGCCCCGCCGCAAGTTCCCAACAGCACCGTCCCGGCCGGGTCCACGGCGACCTGGAAGGA
CAAGAAGCGCTACCTGTGGCTGATCGGGCTGGTGGTGCCGTCGCTGGCCTTCATCGGCTACGGGCTGTGGGCCCTCACCG
GCTGGGGCGTGTGGTTCTGGATCGGGCCGTTCGTGATCCTGGTCGTGGTGCCCGCCATCGACCTGGTCGCGGGGCTGGAC
CGTTCCAACCCGCCCGACGACGTGATCGAGGCGCTCGAGCAGGACCGCTACTACCGCTGGATCACCTACCTGTTCCTGCC
GATCCAGTACGCCGGCTTCGTCGGCGCCATGTGGCTGATCGGCGGCGGGGCGGACCTGGGCACCCTCGACAAGATCGGCC
TGGCGGTCTCGATCGGCTGCATCGGCGGCATCGGCATCAACACCGCCCACGAGCTCGGCCACAAACGGGAGGCCAACGAG
CGCTGGCTGTCCAAGATCGCCCTGGCGCAGAGCTTCTACGGGCACTTCTACATCGAGCACAACCGCGGCCACCACGTGCG
GGTCGCCACGCCCGAGGACCCGGCCAGCAGCCGGGTGGGCGAGAACTTCTACCAGTTCTGGCCGCGCACGGTCCGGGGCT
CGCTGAAGTCCGCGTGGCGCCTGGAGCAGCGCCGCTACGCCCGCAAGCAGCAGCACCCGTTCCGGATCGGCAACGACGTC
CTCAACGCGTGGCTGATGTCGGCGGTGCTGTGGGGCGCGCTGGTCGCCTGGCTCGGCGCCGGCGTGCTGCCGTACCTGGT
GATCCAGGCGGTCGTCGGGTTCTCGCTGCTCGAGGTCGTGAACTACATGGAGCACTACGGGATGCTCCGGCAGAAGGTGG
GCGTCGGCGAGCGGCAGCGCTACGAGCGGGTCGACCCCACCCACTCGTGGAACTCCAACAACATCGCCACCAACGTGCTG
CTCTACCACCTGCAGCGGCACAGCGACCACCACGCGAACCCGACCCGGCGCTACCAGTCGCTGCGCGACTTCGAGGAGTC
GCCGGTGCTCCCGACCGGGTACGCCGGGATGATCGTGCTCGCCATCGTCCCCGCGATCTGGCGCCGGGTGATGGACCCTC
GGGTCCTGGCCCACTTCGACGGCGACCTGTCGCGGGCCAACCTGTCCCCGCGCAAGCGCGACCGGATCCTCGCGAAGTAC
CCGCCGCCGGCCTCGACCGTGGTCGAGGAGGTCGCCCTGCAGCCGTCCCGGAACCCGGTCGCGGACGAGGTCCTGGCCGC
CCGGTGCCCGGGCTGCGGGTACACCTACCGCGTCGAGGAGGGCAACGAGCTCGAGGGCTTGGCGGCCGGCACCGCCTGGG
CCGACGTACCCGACGACTGGTGCTGCCCGGACTGCGGCGTACGCGAGAAGGTCGACTTCGTGCCGCTCACCGAGGCGGCC
GCCTGA

Upstream 100 bases:

>100_bases
TCGGTGGACGTTTGTGCGCTGCAGGTCTAGACAGTTGACATTTCCTGTGGTTTTGTAAAGGCCTGGACGTGACCACCGTC
ATTCGCAGGAGGCCGAGCCC

Downstream 100 bases:

>100_bases
GGCACGGAGCCCGGCCGGGGCCGAGCACCACCGCCGCCGAGGTCTCCTCCTAGACTCGTGAGGTGCCTGGGACCACGCGC
TCGCGGATCGTGGACGCGGC

Product: alkane 1-monooxygenase

Products: NA

Alternate protein names: Alkane hydroxylase; AHs; Terminal alkane hydroxylase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTATPPTAPPQVPNSTVPAGSTATWKDKKRYLWLIGLVVPSLAFIGYGLWALTGWGVWFWIGPFVILVVVPAIDLVAGLD
RSNPPDDVIEALEQDRYYRWITYLFLPIQYAGFVGAMWLIGGGADLGTLDKIGLAVSIGCIGGIGINTAHELGHKREANE
RWLSKIALAQSFYGHFYIEHNRGHHVRVATPEDPASSRVGENFYQFWPRTVRGSLKSAWRLEQRRYARKQQHPFRIGNDV
LNAWLMSAVLWGALVAWLGAGVLPYLVIQAVVGFSLLEVVNYMEHYGMLRQKVGVGERQRYERVDPTHSWNSNNIATNVL
LYHLQRHSDHHANPTRRYQSLRDFEESPVLPTGYAGMIVLAIVPAIWRRVMDPRVLAHFDGDLSRANLSPRKRDRILAKY
PPPASTVVEEVALQPSRNPVADEVLAARCPGCGYTYRVEEGNELEGLAAGTAWADVPDDWCCPDCGVREKVDFVPLTEAA
A

Sequences:

>Translated_481_residues
MTATPPTAPPQVPNSTVPAGSTATWKDKKRYLWLIGLVVPSLAFIGYGLWALTGWGVWFWIGPFVILVVVPAIDLVAGLD
RSNPPDDVIEALEQDRYYRWITYLFLPIQYAGFVGAMWLIGGGADLGTLDKIGLAVSIGCIGGIGINTAHELGHKREANE
RWLSKIALAQSFYGHFYIEHNRGHHVRVATPEDPASSRVGENFYQFWPRTVRGSLKSAWRLEQRRYARKQQHPFRIGNDV
LNAWLMSAVLWGALVAWLGAGVLPYLVIQAVVGFSLLEVVNYMEHYGMLRQKVGVGERQRYERVDPTHSWNSNNIATNVL
LYHLQRHSDHHANPTRRYQSLRDFEESPVLPTGYAGMIVLAIVPAIWRRVMDPRVLAHFDGDLSRANLSPRKRDRILAKY
PPPASTVVEEVALQPSRNPVADEVLAARCPGCGYTYRVEEGNELEGLAAGTAWADVPDDWCCPDCGVREKVDFVPLTEAA
A
>Mature_480_residues
TATPPTAPPQVPNSTVPAGSTATWKDKKRYLWLIGLVVPSLAFIGYGLWALTGWGVWFWIGPFVILVVVPAIDLVAGLDR
SNPPDDVIEALEQDRYYRWITYLFLPIQYAGFVGAMWLIGGGADLGTLDKIGLAVSIGCIGGIGINTAHELGHKREANER
WLSKIALAQSFYGHFYIEHNRGHHVRVATPEDPASSRVGENFYQFWPRTVRGSLKSAWRLEQRRYARKQQHPFRIGNDVL
NAWLMSAVLWGALVAWLGAGVLPYLVIQAVVGFSLLEVVNYMEHYGMLRQKVGVGERQRYERVDPTHSWNSNNIATNVLL
YHLQRHSDHHANPTRRYQSLRDFEESPVLPTGYAGMIVLAIVPAIWRRVMDPRVLAHFDGDLSRANLSPRKRDRILAKYP
PPASTVVEEVALQPSRNPVADEVLAARCPGCGYTYRVEEGNELEGLAAGTAWADVPDDWCCPDCGVREKVDFVPLTEAAA

Specific function: Catalyzes the hydroxylation of n-alkanes in the presence of a NADH-rubredoxin reductase and rubredoxin. It preferably hydroxylases long-chain-length alkanes with at least 12 carbon atoms [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family. AlkB subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005804 [H]

Pfam domain/function: PF00487 FA_desaturase [H]

EC number: =1.14.15.3 [H]

Molecular weight: Translated: 53727; Mature: 53596

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00202 RUBREDOXIN ; PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPPTAPPQVPNSTVPAGSTATWKDKKRYLWLIGLVVPSLAFIGYGLWALTGWGVWFW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGPFVILVVVPAIDLVAGLDRSNPPDDVIEALEQDRYYRWITYLFLPIQYAGFVGAMWLI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GGGADLGTLDKIGLAVSIGCIGGIGINTAHELGHKREANERWLSKIALAQSFYGHFYIEH
CCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCEEEEEC
NRGHHVRVATPEDPASSRVGENFYQFWPRTVRGSLKSAWRLEQRRYARKQQHPFRIGNDV
CCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LNAWLMSAVLWGALVAWLGAGVLPYLVIQAVVGFSLLEVVNYMEHYGMLRQKVGVGERQR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YERVDPTHSWNSNNIATNVLLYHLQRHSDHHANPTRRYQSLRDFEESPVLPTGYAGMIVL
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AIVPAIWRRVMDPRVLAHFDGDLSRANLSPRKRDRILAKYPPPASTVVEEVALQPSRNPV
HHHHHHHHHHCCCCEEECCCCCCHHCCCCHHHHCCEEECCCCCHHHHHHHHHCCCCCCCH
ADEVLAARCPGCGYTYRVEEGNELEGLAAGTAWADVPDDWCCPDCGVREKVDFVPLTEAA
HHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
A
C
>Mature Secondary Structure 
TATPPTAPPQVPNSTVPAGSTATWKDKKRYLWLIGLVVPSLAFIGYGLWALTGWGVWFW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGPFVILVVVPAIDLVAGLDRSNPPDDVIEALEQDRYYRWITYLFLPIQYAGFVGAMWLI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GGGADLGTLDKIGLAVSIGCIGGIGINTAHELGHKREANERWLSKIALAQSFYGHFYIEH
CCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCEEEEEC
NRGHHVRVATPEDPASSRVGENFYQFWPRTVRGSLKSAWRLEQRRYARKQQHPFRIGNDV
CCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LNAWLMSAVLWGALVAWLGAGVLPYLVIQAVVGFSLLEVVNYMEHYGMLRQKVGVGERQR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YERVDPTHSWNSNNIATNVLLYHLQRHSDHHANPTRRYQSLRDFEESPVLPTGYAGMIVL
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AIVPAIWRRVMDPRVLAHFDGDLSRANLSPRKRDRILAKYPPPASTVVEEVALQPSRNPV
HHHHHHHHHHCCCCEEECCCCCCHHCCCCHHHHCCEEECCCCCHHHHHHHHHCCCCCCCH
ADEVLAARCPGCGYTYRVEEGNELEGLAAGTAWADVPDDWCCPDCGVREKVDFVPLTEAA
HHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]