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The map label for this gene is dadA [C]

Identifier: 119714350

GI number: 119714350

Start: 92896

End: 94077

Strand: Direct

Name: dadA [C]

Synonym: Noca_0082

Alternate gene names: 119714350

Gene position: 92896-94077 (Clockwise)

Preceding gene: 119714349

Following gene: 119714351

Centisome position: 1.86

GC content: 73.35

Gene sequence:

>1182_bases
GTGGACTGCGACGTCGTCGTGATCGGCGCGGGCATCGTCGGGGCTGCCTGTGCTCGCCGACTGGCCCGAGCGGGCTTGGC
CGTCACCGTCGTGGACCGTAGCGCGGCCGCAGGGGGCACGACAGCACACGGCGAGGGCAACCTGCTCGTCTCCGACAAGC
GGGCCGGCGCCGAGCTCGACATCGCCCAGTACTCGGCGGAGCTGTGGCGCCAGCTCAGCGTGGAGCTGGCCGACGAGCTC
GGGCCGGAGTTCCCGCCGCTGGAGTTCGAGGAGAAGGGCGGCCTGGTGGTCGCCACCGACGAGCGGGGCGCGGGCCCCCT
CGTCGAGCTGGCGGCGAGTCAGTGTCAGGCGGGGGTTCGGGCCGAGGTGCTCTCGAGCTCCGAGGCCCGACGCCTCGAAC
CCGAGCTGACCCCGTCGACCGTGGCGGCCGTGCACTATCCGGAGGACGCGCAGGTGCAGCCGGTCGTCGCGGCCGAAGCG
CTGCTGGCGTCGGCCCGCCGTGCGGGCGCCCGGATCCTCCCGCACACGGAGGTCACCGGCCCGGTGCTCTCCGAGGGCGG
CCGGTTGGGTGGTGTCACCACGACGGCGGGCCCGATCCGGGCGACGCACGTGGTCCTCGCGGCGGGGCCCTGGTCGGCCG
GAGTCGCCTCGTCCCTCGGCGCCACGATCCCGGTCGTCCCGCGGCGCGGCATGGTGCTGGTGACGACGAGGATGCGGCAC
CGGATCTTCCGGAAGGTCTACGACGCCGACTACGTCGGCGCCGTCGGCTCGAACGACTCTGACCTCCAGACGTCCAGCGT
CATCGAGAGCACGGCGTCCGGCACGGTCCTGATCGGCTCCTCACGGCAGATGATCGGGTTCGACGACTGCCTGCGCGTGC
CGGTCCTCGAGGAGCTGGCGCGCAAGGCCATCGATCTCTTCCCGTTCCTCCGCACCGCCTCGGTCATGCGGGTCTACGGG
GGGTTCCGCCCGTTCACGCCGGACCACCTGCCGCTCATCGGGCCCGACGTCACCGTCCCCGGGCTCTGGCACGCGAACGG
GCACGAAGGAGCCGGCATCGGACTCGCGCCGGCCACGGGCGAGATCCTCGGCGCCCTGGTCGCAGGTGAGCGGCCGGCGG
TCGACGCGTCACCGTATCTGCCGGACCGGGCTGCTCTCGTGCCTGCCAGGGGCGTGCGATGA

Upstream 100 bases:

>100_bases
GAGGGCCGCGCTGGAGCAGGTCGGCTTCTCCGAGGAGGAGACGGCCGGAATCCTCGGCGAGAACTTCCTGCGCGTCTTCG
CCGAGGTCTGGGGAGCCTGA

Downstream 100 bases:

>100_bases
GCGCGTTCCTCCGCCGGGCGGCCGACGACCCGATCCGCCGCCGTGACGGCAAGGCCATCTCCATCCGCGTCGACGGTGAG
CAGCTCACCGGCGTCGAGGG

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MDCDVVVIGAGIVGAACARRLARAGLAVTVVDRSAAAGGTTAHGEGNLLVSDKRAGAELDIAQYSAELWRQLSVELADEL
GPEFPPLEFEEKGGLVVATDERGAGPLVELAASQCQAGVRAEVLSSSEARRLEPELTPSTVAAVHYPEDAQVQPVVAAEA
LLASARRAGARILPHTEVTGPVLSEGGRLGGVTTTAGPIRATHVVLAAGPWSAGVASSLGATIPVVPRRGMVLVTTRMRH
RIFRKVYDADYVGAVGSNDSDLQTSSVIESTASGTVLIGSSRQMIGFDDCLRVPVLEELARKAIDLFPFLRTASVMRVYG
GFRPFTPDHLPLIGPDVTVPGLWHANGHEGAGIGLAPATGEILGALVAGERPAVDASPYLPDRAALVPARGVR

Sequences:

>Translated_393_residues
MDCDVVVIGAGIVGAACARRLARAGLAVTVVDRSAAAGGTTAHGEGNLLVSDKRAGAELDIAQYSAELWRQLSVELADEL
GPEFPPLEFEEKGGLVVATDERGAGPLVELAASQCQAGVRAEVLSSSEARRLEPELTPSTVAAVHYPEDAQVQPVVAAEA
LLASARRAGARILPHTEVTGPVLSEGGRLGGVTTTAGPIRATHVVLAAGPWSAGVASSLGATIPVVPRRGMVLVTTRMRH
RIFRKVYDADYVGAVGSNDSDLQTSSVIESTASGTVLIGSSRQMIGFDDCLRVPVLEELARKAIDLFPFLRTASVMRVYG
GFRPFTPDHLPLIGPDVTVPGLWHANGHEGAGIGLAPATGEILGALVAGERPAVDASPYLPDRAALVPARGVR
>Mature_393_residues
MDCDVVVIGAGIVGAACARRLARAGLAVTVVDRSAAAGGTTAHGEGNLLVSDKRAGAELDIAQYSAELWRQLSVELADEL
GPEFPPLEFEEKGGLVVATDERGAGPLVELAASQCQAGVRAEVLSSSEARRLEPELTPSTVAAVHYPEDAQVQPVVAAEA
LLASARRAGARILPHTEVTGPVLSEGGRLGGVTTTAGPIRATHVVLAAGPWSAGVASSLGATIPVVPRRGMVLVTTRMRH
RIFRKVYDADYVGAVGSNDSDLQTSSVIESTASGTVLIGSSRQMIGFDDCLRVPVLEELARKAIDLFPFLRTASVMRVYG
GFRPFTPDHLPLIGPDVTVPGLWHANGHEGAGIGLAPATGEILGALVAGERPAVDASPYLPDRAALVPARGVR

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=388, Percent_Identity=26.5463917525773, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21361378, Length=388, Percent_Identity=26.5463917525773, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24797151, Length=399, Percent_Identity=25.062656641604, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194306651, Length=383, Percent_Identity=23.2375979112272, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787438, Length=424, Percent_Identity=25, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788574, Length=332, Percent_Identity=29.5180722891566, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130091, Length=376, Percent_Identity=28.4574468085106, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40739; Mature: 40739

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCDVVVIGAGIVGAACARRLARAGLAVTVVDRSAAAGGTTAHGEGNLLVSDKRAGAELD
CCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEH
IAQYSAELWRQLSVELADELGPEFPPLEFEEKGGLVVATDERGAGPLVELAASQCQAGVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
AEVLSSSEARRLEPELTPSTVAAVHYPEDAQVQPVVAAEALLASARRAGARILPHTEVTG
HHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCC
PVLSEGGRLGGVTTTAGPIRATHVVLAAGPWSAGVASSLGATIPVVPRRGMVLVTTRMRH
CHHHCCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHCCCCEEECCCCCEEEEEHHHHH
RIFRKVYDADYVGAVGSNDSDLQTSSVIESTASGTVLIGSSRQMIGFDDCLRVPVLEELA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCEECHHHHHCCHHHHHHH
RKAIDLFPFLRTASVMRVYGGFRPFTPDHLPLIGPDVTVPGLWHANGHEGAGIGLAPATG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
EILGALVAGERPAVDASPYLPDRAALVPARGVR
HHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MDCDVVVIGAGIVGAACARRLARAGLAVTVVDRSAAAGGTTAHGEGNLLVSDKRAGAELD
CCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEH
IAQYSAELWRQLSVELADELGPEFPPLEFEEKGGLVVATDERGAGPLVELAASQCQAGVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
AEVLSSSEARRLEPELTPSTVAAVHYPEDAQVQPVVAAEALLASARRAGARILPHTEVTG
HHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCC
PVLSEGGRLGGVTTTAGPIRATHVVLAAGPWSAGVASSLGATIPVVPRRGMVLVTTRMRH
CHHHCCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHCCCCEEECCCCCEEEEEHHHHH
RIFRKVYDADYVGAVGSNDSDLQTSSVIESTASGTVLIGSSRQMIGFDDCLRVPVLEELA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCEECHHHHHCCHHHHHHH
RKAIDLFPFLRTASVMRVYGGFRPFTPDHLPLIGPDVTVPGLWHANGHEGAGIGLAPATG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
EILGALVAGERPAVDASPYLPDRAALVPARGVR
HHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]