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The map label for this gene is purR [H]

Identifier: 119714329

GI number: 119714329

Start: 65532

End: 66548

Strand: Direct

Name: purR [H]

Synonym: Noca_0058

Alternate gene names: 119714329

Gene position: 65532-66548 (Clockwise)

Preceding gene: 119714326

Following gene: 119714330

Centisome position: 1.31

GC content: 68.73

Gene sequence:

>1017_bases
ATGGCCACGATCGTGGAGGTTGCGCGGCTGGCGGGTGTGTCGACGTCGACCGTCTCTCACGTCCTGAACGGCACGCGGAA
TGTCGAGCCGAAGACGCGCAAGAAGGTCGTCGAGGCGATCGAGAAGACCGGCTACCGACAGGACGCTGTCGCGCGCGCCA
TGCGCCGTTCGCGGACCGACAGCATCGGCCTGGTCGTCTCCGATGCCGGGGAGCCGGCCTTCGCGGACATGGTCCACGGG
GTGGAGCACGCGGCCGCCGCGCGCGGGCTCACGCTCCTCCTTGCGAACTCTGCTGAAGATCCGGAGCGCGAGACTCGCGC
GGTCCGAACGCTCCTCCAGCGGCGGGTCGACGGGTTGATCGTGGCGCGTTGCCCGCGCTCGTCGAGTGAGGTGGAGGGCC
AGATGTCGCACGCGACGCCGCCTGTCGTGCTGCTCGACCGCGTCTTCGATAGAGCCCCCTGCGATCAGGTCGGTGCCGAC
AACCGCGAGAGCATGCGACGCCTGGCTGAGCACCTGCTCGGCCGCGGCCACCGCCGGTTGGTGGTCGTGGCTGGTGACAC
GCGTGTCCCGACCCTCACCGAGCGCCTCGCTGGCTTCAGGGACGCGATTGCGGCGAGGTCTGATGTCGACGCAGCCATCT
TGGAGGGGGCGGAGGCGGAGCCGCTTAAGGCAGGCCTCGTTGAGTCCATCGCTGCCGGTGGCCGAACGTGCGTCATCACG
GCCAGCAGTCCCCTGGCCGTGATGGCCCTGCAGTCGCTGCGCGACCTGCACCTGGCCGTCCCAGAAGACATCGCATTCGC
GACGTTCGACGGTTTCCATCACTCGGATCTGTTCCGGCCCTCGATCACGACCGTGCGCCAGCCTGCTTTCGACATGGGGG
TTGCCGCCGTCGGGCTCCTGCTGGAGCGGCTCGAGTCGCCCGACGCCAGCCCGCGGATTACCCGACTGCAGCAACGTCTC
GAGCTGCGGTCGTCGAGCGAGGACTACGTCTTCGCTGCTCTCGGCGCAGGGAACTAG

Upstream 100 bases:

>100_bases
TGCCGTGCGCAACGGCGGGCCAACCCGCTATGTTGAACAGCAGGAAGACGACGCAAGGGCCAACTGGCGGGCGACGTACA
AACGTTTGAATGGAGCCTTT

Downstream 100 bases:

>100_bases
CTGGTCCGGTGGAACCGCTTGACACATGTGACGGCCGACATTACGTTGGGCGAAACGATTCGCGCAAACGTTTCGATATC
TCGGTGATCTAGTCCCACTT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MATIVEVARLAGVSTSTVSHVLNGTRNVEPKTRKKVVEAIEKTGYRQDAVARAMRRSRTDSIGLVVSDAGEPAFADMVHG
VEHAAAARGLTLLLANSAEDPERETRAVRTLLQRRVDGLIVARCPRSSSEVEGQMSHATPPVVLLDRVFDRAPCDQVGAD
NRESMRRLAEHLLGRGHRRLVVVAGDTRVPTLTERLAGFRDAIAARSDVDAAILEGAEAEPLKAGLVESIAAGGRTCVIT
ASSPLAVMALQSLRDLHLAVPEDIAFATFDGFHHSDLFRPSITTVRQPAFDMGVAAVGLLLERLESPDASPRITRLQQRL
ELRSSSEDYVFAALGAGN

Sequences:

>Translated_338_residues
MATIVEVARLAGVSTSTVSHVLNGTRNVEPKTRKKVVEAIEKTGYRQDAVARAMRRSRTDSIGLVVSDAGEPAFADMVHG
VEHAAAARGLTLLLANSAEDPERETRAVRTLLQRRVDGLIVARCPRSSSEVEGQMSHATPPVVLLDRVFDRAPCDQVGAD
NRESMRRLAEHLLGRGHRRLVVVAGDTRVPTLTERLAGFRDAIAARSDVDAAILEGAEAEPLKAGLVESIAAGGRTCVIT
ASSPLAVMALQSLRDLHLAVPEDIAFATFDGFHHSDLFRPSITTVRQPAFDMGVAAVGLLLERLESPDASPRITRLQQRL
ELRSSSEDYVFAALGAGN
>Mature_337_residues
ATIVEVARLAGVSTSTVSHVLNGTRNVEPKTRKKVVEAIEKTGYRQDAVARAMRRSRTDSIGLVVSDAGEPAFADMVHGV
EHAAAARGLTLLLANSAEDPERETRAVRTLLQRRVDGLIVARCPRSSSEVEGQMSHATPPVVLLDRVFDRAPCDQVGADN
RESMRRLAEHLLGRGHRRLVVVAGDTRVPTLTERLAGFRDAIAARSDVDAAILEGAEAEPLKAGLVESIAAGGRTCVITA
SSPLAVMALQSLRDLHLAVPEDIAFATFDGFHHSDLFRPSITTVRQPAFDMGVAAVGLLLERLESPDASPRITRLQQRLE
LRSSSEDYVFAALGAGN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=33.033033033033, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=31.9526627218935, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=28.0757097791798, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=31.6498316498317, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=30.718954248366, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790369, Length=317, Percent_Identity=27.4447949526814, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1786268, Length=333, Percent_Identity=29.4294294294294, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787580, Length=338, Percent_Identity=26.3313609467456, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787906, Length=197, Percent_Identity=32.994923857868, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=24.2138364779874, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=25.8566978193146, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36331; Mature: 36200

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIVEVARLAGVSTSTVSHVLNGTRNVEPKTRKKVVEAIEKTGYRQDAVARAMRRSRTD
CCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
SIGLVVSDAGEPAFADMVHGVEHAAAARGLTLLLANSAEDPERETRAVRTLLQRRVDGLI
CCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VARCPRSSSEVEGQMSHATPPVVLLDRVFDRAPCDQVGADNRESMRRLAEHLLGRGHRRL
EEECCCCCHHHCCHHHCCCCHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEE
VVVAGDTRVPTLTERLAGFRDAIAARSDVDAAILEGAEAEPLKAGLVESIAAGGRTCVIT
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
ASSPLAVMALQSLRDLHLAVPEDIAFATFDGFHHSDLFRPSITTVRQPAFDMGVAAVGLL
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHH
LERLESPDASPRITRLQQRLELRSSSEDYVFAALGAGN
HHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure 
ATIVEVARLAGVSTSTVSHVLNGTRNVEPKTRKKVVEAIEKTGYRQDAVARAMRRSRTD
CHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
SIGLVVSDAGEPAFADMVHGVEHAAAARGLTLLLANSAEDPERETRAVRTLLQRRVDGLI
CCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VARCPRSSSEVEGQMSHATPPVVLLDRVFDRAPCDQVGADNRESMRRLAEHLLGRGHRRL
EEECCCCCHHHCCHHHCCCCHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEE
VVVAGDTRVPTLTERLAGFRDAIAARSDVDAAILEGAEAEPLKAGLVESIAAGGRTCVIT
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
ASSPLAVMALQSLRDLHLAVPEDIAFATFDGFHHSDLFRPSITTVRQPAFDMGVAAVGLL
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHH
LERLESPDASPRITRLQQRLELRSSSEDYVFAALGAGN
HHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA