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The map label for this gene is dnaN [H]

Identifier: 119714274

GI number: 119714274

Start: 2029

End: 3255

Strand: Direct

Name: dnaN [H]

Synonym: Noca_0002

Alternate gene names: 119714274

Gene position: 2029-3255 (Clockwise)

Preceding gene: 119714273

Following gene: 119714275

Centisome position: 0.04

GC content: 71.39

Gene sequence:

>1227_bases
ATGCAGAGCCCAGAACGTGCACTTCTCCCACCCCCGTTCAACCCCCGCTCAACCCTCGCTCCACCGAGCCGACCCGAAGG
AACCATCGTGAAGTTCCGCGTCGAGCGCGACGTGCTCGCCGATGCCGTCGCCTGGGCTGCCCGCAGCCTCCCGGTCCGTC
CCAGCGTCCCCGTCCTCGCCGGCCTGCTCGTCGAGGCGAGCGACGACGGCCTGGTGCTCTCCACCTTCGACTACGAGACC
TCCGCCCGGGCCACCCTGACGGCCGAGGTGAGCGACGAGGGGCAGGCCCTGGTCAGTGGCCGACTGCTCGCCGACATCTG
CCGGAGCCTGCCCGCGAAGCCCGTCGAGATGGTCCTCGACGGGGCCCGGGTGTCGCTGACCTGTGGCTCGGCGCGGTTCA
GCCTGCAGACGATGCCGGTTGACGACTACCCGTCCCTGCCGAGCATGCCCGCGGCGACCGGCACCGTGCAGAGCGAGGCC
TTTGCACACGCCGTCGCCCAGGCCGTGACGGCGGCGGGTCGCGACGACATGCTGCCCGTGCTCACCGGCGTGCGGATCGA
GATCGACGGCTCGACCATCTCGCTGCTCGCCACCGACCGGTTCCGCCTCTCCCACCGGGAGCTGGAGTGGAGTCCGCGGA
CCCCGGACGAGTCGGCGGCCGCCCTGGTCCCGGCACGGGTCCTCGGCGACACCGCCAAGTCCCTGACCGGGGGCGGCGAG
GTCACGATCGCGCTGGCGACCACCGGCTCCGGCGAGGGCATCATCGGCTTCGAGGGCCATGGTCCCGGCGGACTGCGTCG
GACCACGACCCGGCTGCTCGACGGCGAGTTCCCGAAGGTCCGCAGCCTGTTCCCGAACGAGCACCAGACCACGGCGCTGG
TCGACAAGGCCGCGCTCGTCGAGTCGGTGAAGCGGGTGGCGCTGGTCGCCGAGCGCAACACCGCGGTCCAGCTCGCCTTC
AGCGACGGCGTACTGACCCTCGACGCCGGGTCGGGCGACGAGGCCCAGGCCTCGGAGTCGATCGAGGCGACCATCGACGG
TGAGGACATCACCACGGGCTTCAACCCGCAGTTCCTGCTCGACGGCCTGACCGCCATCGACCAGCCGGTGGTCGAGCTCG
CCTTCACCCAGGCCTCCAAGCCGGTGGTGATCAGCGGCGCGGTCGACGGCGACGACACCGACTCGTCATTCCGCTACCTG
CTGATGCCGCGCCGCCTGCTCTCCTAG

Upstream 100 bases:

>100_bases
CTACGACTCCCACCTACCTCCAGACAGATCGATCCTCGGTGAACATCCATCCGGGCCCCATCCTGGGGACAACTCCCGCG
CGCCGGCGCCGCATGGCAGG

Downstream 100 bases:

>100_bases
GGCCACGCGCTCGCTGGGCCGTTCGGGTACGGGCCCTAGGCTGGGGCCGACCGCGCCACGGCAGGGAGGAACTGCGATGC
ACATCGGACTCGTCGGCCTC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQSPERALLPPPFNPRSTLAPPSRPEGTIVKFRVERDVLADAVAWAARSLPVRPSVPVLAGLLVEASDDGLVLSTFDYET
SARATLTAEVSDEGQALVSGRLLADICRSLPAKPVEMVLDGARVSLTCGSARFSLQTMPVDDYPSLPSMPAATGTVQSEA
FAHAVAQAVTAAGRDDMLPVLTGVRIEIDGSTISLLATDRFRLSHRELEWSPRTPDESAAALVPARVLGDTAKSLTGGGE
VTIALATTGSGEGIIGFEGHGPGGLRRTTTRLLDGEFPKVRSLFPNEHQTTALVDKAALVESVKRVALVAERNTAVQLAF
SDGVLTLDAGSGDEAQASESIEATIDGEDITTGFNPQFLLDGLTAIDQPVVELAFTQASKPVVISGAVDGDDTDSSFRYL
LMPRRLLS

Sequences:

>Translated_408_residues
MQSPERALLPPPFNPRSTLAPPSRPEGTIVKFRVERDVLADAVAWAARSLPVRPSVPVLAGLLVEASDDGLVLSTFDYET
SARATLTAEVSDEGQALVSGRLLADICRSLPAKPVEMVLDGARVSLTCGSARFSLQTMPVDDYPSLPSMPAATGTVQSEA
FAHAVAQAVTAAGRDDMLPVLTGVRIEIDGSTISLLATDRFRLSHRELEWSPRTPDESAAALVPARVLGDTAKSLTGGGE
VTIALATTGSGEGIIGFEGHGPGGLRRTTTRLLDGEFPKVRSLFPNEHQTTALVDKAALVESVKRVALVAERNTAVQLAF
SDGVLTLDAGSGDEAQASESIEATIDGEDITTGFNPQFLLDGLTAIDQPVVELAFTQASKPVVISGAVDGDDTDSSFRYL
LMPRRLLS
>Mature_408_residues
MQSPERALLPPPFNPRSTLAPPSRPEGTIVKFRVERDVLADAVAWAARSLPVRPSVPVLAGLLVEASDDGLVLSTFDYET
SARATLTAEVSDEGQALVSGRLLADICRSLPAKPVEMVLDGARVSLTCGSARFSLQTMPVDDYPSLPSMPAATGTVQSEA
FAHAVAQAVTAAGRDDMLPVLTGVRIEIDGSTISLLATDRFRLSHRELEWSPRTPDESAAALVPARVLGDTAKSLTGGGE
VTIALATTGSGEGIIGFEGHGPGGLRRTTTRLLDGEFPKVRSLFPNEHQTTALVDKAALVESVKRVALVAERNTAVQLAF
SDGVLTLDAGSGDEAQASESIEATIDGEDITTGFNPQFLLDGLTAIDQPVVELAFTQASKPVVISGAVDGDDTDSSFRYL
LMPRRLLS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=392, Percent_Identity=27.8061224489796, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42955; Mature: 42955

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPERALLPPPFNPRSTLAPPSRPEGTIVKFRVERDVLADAVAWAARSLPVRPSVPVLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHE
GLLVEASDDGLVLSTFDYETSARATLTAEVSDEGQALVSGRLLADICRSLPAKPVEMVLD
EEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCHHHHCC
GARVSLTCGSARFSLQTMPVDDYPSLPSMPAATGTVQSEAFAHAVAQAVTAAGRDDMLPV
CCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
LTGVRIEIDGSTISLLATDRFRLSHRELEWSPRTPDESAAALVPARVLGDTAKSLTGGGE
HCCEEEEECCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
VTIALATTGSGEGIIGFEGHGPGGLRRTTTRLLDGEFPKVRSLFPNEHQTTALVDKAALV
EEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHH
ESVKRVALVAERNTAVQLAFSDGVLTLDAGSGDEAQASESIEATIDGEDITTGFNPQFLL
HHHHHHEEEEECCCEEEEEEECCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
DGLTAIDQPVVELAFTQASKPVVISGAVDGDDTDSSFRYLLMPRRLLS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCHHHCC
>Mature Secondary Structure
MQSPERALLPPPFNPRSTLAPPSRPEGTIVKFRVERDVLADAVAWAARSLPVRPSVPVLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHE
GLLVEASDDGLVLSTFDYETSARATLTAEVSDEGQALVSGRLLADICRSLPAKPVEMVLD
EEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCHHHHCC
GARVSLTCGSARFSLQTMPVDDYPSLPSMPAATGTVQSEAFAHAVAQAVTAAGRDDMLPV
CCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHH
LTGVRIEIDGSTISLLATDRFRLSHRELEWSPRTPDESAAALVPARVLGDTAKSLTGGGE
HCCEEEEECCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
VTIALATTGSGEGIIGFEGHGPGGLRRTTTRLLDGEFPKVRSLFPNEHQTTALVDKAALV
EEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHH
ESVKRVALVAERNTAVQLAFSDGVLTLDAGSGDEAQASESIEATIDGEDITTGFNPQFLL
HHHHHHEEEEECCCEEEEEEECCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
DGLTAIDQPVVELAFTQASKPVVISGAVDGDDTDSSFRYLLMPRRLLS
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1577691; 12000953 [H]