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The map label for this gene is cysD [H]

Identifier: 119387100

GI number: 119387100

Start: 1589413

End: 1590348

Strand: Direct

Name: cysD [H]

Synonym: Pden_4395

Alternate gene names: 119387100

Gene position: 1589413-1590348 (Clockwise)

Preceding gene: 119387099

Following gene: 119387101

Centisome position: 91.87

GC content: 63.35

Gene sequence:

>936_bases
ATGAACAGCCAATCCGTCATTCATACCGGGGGTCAGGCGGCCACGCTGACCCATCTGCAGCGGCTGGAGGCCGAAAGCAT
CCAGATCATGCGCGAGGTCGTGGCTACGGCCGACAACCCGGTGATGCTGTATTCGGTGGGCAAGGATTCGGCCTGCATGC
TGCATCTGGCGAAGAAGGCGTTCTATCCGGCCCCGCCGCCCTTTCCGCTGCTGCATGTGGACACCACCTGGAAATTCCGC
GCCATGTACGAGCTGCGCGACCGCGCCGCGCGCGACGCCGGCATGGAGCTGATCATCCATCACAACCCCGAGGCGATGGA
AAAGGGCATCAACCCCTTCGACCATGGCAGCCTGCATACCGACATGTGGAAGACGGACGGGCTGAAGCAGGCGCTGAGCA
AGTTCAATTTCGACGTGGCCTTCGGCGGCGCGCGGCGCGACGAAGAGAAATCGCGCGCCAAGGAGCGGGTGTTCTCGTTC
CGCTCGGCCAATCACCGCTGGGACCCCAAGAACCAGCGCCCCGAGCTGTGGAAGCTTTACAACACCCGCAAGGGCAAGGG
CGAATCGATCCGGGTCTTCCCGCTGTCGAACTGGACCGAGCTGGACATCTGGCAATACATCCACCTGGAAAACATCGACA
TCGTGCCCCTGTATTTCAGCGAGCCGCGGCCGGTCGTGGAACGCGACGGGCTGCTGATCATGGTCGATGACGAACGCTTT
CCGCTGCGCGAGGGCGAGGTGCCGACGATGAGATCGGTGCGCTTCCGCACGCTGGGCTGCTATCCGCTGACCGGCGCGGT
GGAATCCAGCGCCCGCACCCTGCCCGAGGTCATCCAGGAAATGCTGCTGACCACCACGTCCGAGCGGCAGGGCCGCGCCA
TCGACCACGACCAGTCGGCCTCGATGGAGAAGAAGAAGCAAGAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
GTCCTTTCCGTCGTCGCTCCGCCGTTTCAGGCCCCGTTGGTCTTGGTCTTGCTGCAAAAATCTGGCAAAGGACGCCCATT
GTAGAATAAGGGCCACCCAG

Downstream 100 bases:

>100_bases
GATGAACCACGAGATGAAAGACCCCGTCTACGTCACGGATTCGCTGATCGCCGAGGACATCGACGCCTATCTGCTGAAGC
ACCAGCACAAGACCATGCTG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNSQSVIHTGGQAATLTHLQRLEAESIQIMREVVATADNPVMLYSVGKDSACMLHLAKKAFYPAPPPFPLLHVDTTWKFR
AMYELRDRAARDAGMELIIHHNPEAMEKGINPFDHGSLHTDMWKTDGLKQALSKFNFDVAFGGARRDEEKSRAKERVFSF
RSANHRWDPKNQRPELWKLYNTRKGKGESIRVFPLSNWTELDIWQYIHLENIDIVPLYFSEPRPVVERDGLLIMVDDERF
PLREGEVPTMRSVRFRTLGCYPLTGAVESSARTLPEVIQEMLLTTTSERQGRAIDHDQSASMEKKKQEGYF

Sequences:

>Translated_311_residues
MNSQSVIHTGGQAATLTHLQRLEAESIQIMREVVATADNPVMLYSVGKDSACMLHLAKKAFYPAPPPFPLLHVDTTWKFR
AMYELRDRAARDAGMELIIHHNPEAMEKGINPFDHGSLHTDMWKTDGLKQALSKFNFDVAFGGARRDEEKSRAKERVFSF
RSANHRWDPKNQRPELWKLYNTRKGKGESIRVFPLSNWTELDIWQYIHLENIDIVPLYFSEPRPVVERDGLLIMVDDERF
PLREGEVPTMRSVRFRTLGCYPLTGAVESSARTLPEVIQEMLLTTTSERQGRAIDHDQSASMEKKKQEGYF
>Mature_311_residues
MNSQSVIHTGGQAATLTHLQRLEAESIQIMREVVATADNPVMLYSVGKDSACMLHLAKKAFYPAPPPFPLLHVDTTWKFR
AMYELRDRAARDAGMELIIHHNPEAMEKGINPFDHGSLHTDMWKTDGLKQALSKFNFDVAFGGARRDEEKSRAKERVFSF
RSANHRWDPKNQRPELWKLYNTRKGKGESIRVFPLSNWTELDIWQYIHLENIDIVPLYFSEPRPVVERDGLLIMVDDERF
PLREGEVPTMRSVRFRTLGCYPLTGAVESSARTLPEVIQEMLLTTTSERQGRAIDHDQSASMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=71.7171717171717, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35775; Mature: 35775

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQSVIHTGGQAATLTHLQRLEAESIQIMREVVATADNPVMLYSVGKDSACMLHLAKKA
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
FYPAPPPFPLLHVDTTWKFRAMYELRDRAARDAGMELIIHHNPEAMEKGINPFDHGSLHT
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCC
DMWKTDGLKQALSKFNFDVAFGGARRDEEKSRAKERVFSFRSANHRWDPKNQRPELWKLY
HHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
NTRKGKGESIRVFPLSNWTELDIWQYIHLENIDIVPLYFSEPRPVVERDGLLIMVDDERF
CCCCCCCCEEEEEECCCCCEECCEEEEEECCCEEEEEEECCCCCCHHCCCEEEEECCCCC
PLREGEVPTMRSVRFRTLGCYPLTGAVESSARTLPEVIQEMLLTTTSERQGRAIDHDQSA
CCCCCCCCCHHHEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
SMEKKKQEGYF
HHHHHHHCCCC
>Mature Secondary Structure
MNSQSVIHTGGQAATLTHLQRLEAESIQIMREVVATADNPVMLYSVGKDSACMLHLAKKA
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
FYPAPPPFPLLHVDTTWKFRAMYELRDRAARDAGMELIIHHNPEAMEKGINPFDHGSLHT
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCC
DMWKTDGLKQALSKFNFDVAFGGARRDEEKSRAKERVFSFRSANHRWDPKNQRPELWKLY
HHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
NTRKGKGESIRVFPLSNWTELDIWQYIHLENIDIVPLYFSEPRPVVERDGLLIMVDDERF
CCCCCCCCEEEEEECCCCCEECCEEEEEECCCEEEEEEECCCCCCHHCCCEEEEECCCCC
PLREGEVPTMRSVRFRTLGCYPLTGAVESSARTLPEVIQEMLLTTTSERQGRAIDHDQSA
CCCCCCCCCHHHEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
SMEKKKQEGYF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]