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The map label for this gene is guaD [H]

Identifier: 119386965

GI number: 119386965

Start: 1427539

End: 1428816

Strand: Direct

Name: guaD [H]

Synonym: Pden_4260

Alternate gene names: 119386965

Gene position: 1427539-1428816 (Clockwise)

Preceding gene: 119386964

Following gene: 119386978

Centisome position: 82.51

GC content: 66.9

Gene sequence:

>1278_bases
ATGCGACAGCTGATCCGGGGGCGCACGCTCTCCTTCCACGCCGACCCGGTCGAGGTCGAGAACGCCTTCACCTATCACGA
GGACGGCGCCATCATCACCCAAGACGGCAAGATCGTCGCCATCGGCGACTATGCCGCGCTGCACGAGCCGGGCCTGCCGG
AAATCGACCACCGGCCGCACCTGATCCTGCCGGGCTTCATCGACACCCATATCCATTTCCCGCAGGTGCAGGTGATCGCC
AGCTGGGGGGCGCAGCTTCTCGACTGGCTCAACACCTATACCTTCCCCGAGGAATCGCGCTTTGCCCAACAGGGCCATGC
GCCGCTGATGGCCGAAAAGTTCCTCGACCTGCTCCTCCAGCACGGCACGACCACCGCCGTCGCCTTCTGCTCGGTGCATC
CGCAATCGGCCGAGGCGCTGTTCTCGGCCGCCGAGGCGCGGGGCATGGCGATGATCGCCGGCAAGGTGATGATGGACCGC
AACGCCCCCGAGGCGGTGCTGGACACGCCGCAGCAGGGCTATGACGACAGCAAGCGGCTCATCGAGAAATGGCACGGCCG
GGGCCGCCAGCGCTATGCCATCACCCCGCGCTTCGCCATCACCTCGACGCCCGAACAGATGCGGATGACCGGGCAACTGG
TGCGCGAACATCCCGACTGCCACATCCAGACGCATCTGTCCGAGAACCGCGACGAGATCGCCTTCACCCTCAGCCTCTAT
CCGCAGGCGCGCGACTATCTCGACATCTACGAGACCTACGGGCTCCTGTCGGACAAGCTGCTGCTCGGCCATTCCATCCA
CCTGGAGCCGCGCGAGATCGCCCGCATGGCCGAGACCGGCAGCCGGGCGGTGTTCTGCCCGACCTCGAACCTGTTCCTCG
GCTCGGGGCTTTTCGACGAGGCGGGCTTGCGCGCGGCGGGCGTGGTCAGCGGCATCGCCACCGATGTCGGCGGCGGCACC
AGCTATTCCATGCTGCAAACCCTGAACGAGGGCTACAAGATCCTGCAACTGCGCGGCCAGAAGCTGCATCCATTCGCCGC
CTTCCATTGGGTCACGCGCGGCAATGCGCTGGCGCTCGGCATGGCCGAGCGGATCGGCACGCTGGACCCCGGCAGCGATG
CCGACCTGGTGGTGCTGGACAGCCGCGCCACGCCGGCGATGGCGCTGCGCATGGACCGCGCCGAAACCCTGGCCGAAGAG
CTGTTCATCCTGCAGATCCTCGGCGACGACCGCGCCATCGCGCAGACCTATGTCGCGGGGAAACCCATGCTCGGCTGA

Upstream 100 bases:

>100_bases
GCTGGTCGCGCGACAAGCGCCCCGAGGTCATCGCCGCCTTCACCGCCGCCGAGATCATCGGCCGGCTGACCGAAACCGTT
TCACCGTTCGAAAAATACTC

Downstream 100 bases:

>100_bases
ACCGGGTCCATGGGCCGGGACATCGGCCGGGTCGTCGCAGATCCCGCAATCCCAGCCGTTTACGACAGGCGCCTTCATGG
TTCCCGTGGGGGGCGGCGGG

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRQLIRGRTLSFHADPVEVENAFTYHEDGAIITQDGKIVAIGDYAALHEPGLPEIDHRPHLILPGFIDTHIHFPQVQVIA
SWGAQLLDWLNTYTFPEESRFAQQGHAPLMAEKFLDLLLQHGTTTAVAFCSVHPQSAEALFSAAEARGMAMIAGKVMMDR
NAPEAVLDTPQQGYDDSKRLIEKWHGRGRQRYAITPRFAITSTPEQMRMTGQLVREHPDCHIQTHLSENRDEIAFTLSLY
PQARDYLDIYETYGLLSDKLLLGHSIHLEPREIARMAETGSRAVFCPTSNLFLGSGLFDEAGLRAAGVVSGIATDVGGGT
SYSMLQTLNEGYKILQLRGQKLHPFAAFHWVTRGNALALGMAERIGTLDPGSDADLVVLDSRATPAMALRMDRAETLAEE
LFILQILGDDRAIAQTYVAGKPMLG

Sequences:

>Translated_425_residues
MRQLIRGRTLSFHADPVEVENAFTYHEDGAIITQDGKIVAIGDYAALHEPGLPEIDHRPHLILPGFIDTHIHFPQVQVIA
SWGAQLLDWLNTYTFPEESRFAQQGHAPLMAEKFLDLLLQHGTTTAVAFCSVHPQSAEALFSAAEARGMAMIAGKVMMDR
NAPEAVLDTPQQGYDDSKRLIEKWHGRGRQRYAITPRFAITSTPEQMRMTGQLVREHPDCHIQTHLSENRDEIAFTLSLY
PQARDYLDIYETYGLLSDKLLLGHSIHLEPREIARMAETGSRAVFCPTSNLFLGSGLFDEAGLRAAGVVSGIATDVGGGT
SYSMLQTLNEGYKILQLRGQKLHPFAAFHWVTRGNALALGMAERIGTLDPGSDADLVVLDSRATPAMALRMDRAETLAEE
LFILQILGDDRAIAQTYVAGKPMLG
>Mature_425_residues
MRQLIRGRTLSFHADPVEVENAFTYHEDGAIITQDGKIVAIGDYAALHEPGLPEIDHRPHLILPGFIDTHIHFPQVQVIA
SWGAQLLDWLNTYTFPEESRFAQQGHAPLMAEKFLDLLLQHGTTTAVAFCSVHPQSAEALFSAAEARGMAMIAGKVMMDR
NAPEAVLDTPQQGYDDSKRLIEKWHGRGRQRYAITPRFAITSTPEQMRMTGQLVREHPDCHIQTHLSENRDEIAFTLSLY
PQARDYLDIYETYGLLSDKLLLGHSIHLEPREIARMAETGSRAVFCPTSNLFLGSGLFDEAGLRAAGVVSGIATDVGGGT
SYSMLQTLNEGYKILQLRGQKLHPFAAFHWVTRGNALALGMAERIGTLDPGSDADLVVLDSRATPAMALRMDRAETLAEE
LFILQILGDDRAIAQTYVAGKPMLG

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=416, Percent_Identity=30.5288461538462, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI1789249, Length=414, Percent_Identity=48.792270531401, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319963, Length=335, Percent_Identity=32.8358208955224, Blast_Score=179, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24643849, Length=431, Percent_Identity=32.9466357308585, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 46941; Mature: 46941

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLIRGRTLSFHADPVEVENAFTYHEDGAIITQDGKIVAIGDYAALHEPGLPEIDHRPH
CCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEECCCEEEEECHHHHCCCCCCCCCCCCC
LILPGFIDTHIHFPQVQVIASWGAQLLDWLNTYTFPEESRFAQQGHAPLMAEKFLDLLLQ
EEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
HGTTTAVAFCSVHPQSAEALFSAAEARGMAMIAGKVMMDRNAPEAVLDTPQQGYDDSKRL
CCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCEECCCCCHHHHCCCCCCCCHHHHH
IEKWHGRGRQRYAITPRFAITSTPEQMRMTGQLVREHPDCHIQTHLSENRDEIAFTLSLY
HHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEC
PQARDYLDIYETYGLLSDKLLLGHSIHLEPREIARMAETGSRAVFCPTSNLFLGSGLFDE
CCCHHHHHHHHHHHHHHCCEEECCEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCC
AGLRAAGVVSGIATDVGGGTSYSMLQTLNEGYKILQLRGQKLHPFAAFHWVTRGNALALG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCEEEEE
MAERIGTLDPGSDADLVVLDSRATPAMALRMDRAETLAEELFILQILGDDRAIAQTYVAG
HHHHCCCCCCCCCCCEEEEECCCCCHHHEEHHHHHHHHHHHEEEEEECCCHHHHHHHHCC
KPMLG
CCCCC
>Mature Secondary Structure
MRQLIRGRTLSFHADPVEVENAFTYHEDGAIITQDGKIVAIGDYAALHEPGLPEIDHRPH
CCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEECCCEEEEECHHHHCCCCCCCCCCCCC
LILPGFIDTHIHFPQVQVIASWGAQLLDWLNTYTFPEESRFAQQGHAPLMAEKFLDLLLQ
EEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
HGTTTAVAFCSVHPQSAEALFSAAEARGMAMIAGKVMMDRNAPEAVLDTPQQGYDDSKRL
CCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCEECCCCCHHHHCCCCCCCCHHHHH
IEKWHGRGRQRYAITPRFAITSTPEQMRMTGQLVREHPDCHIQTHLSENRDEIAFTLSLY
HHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEC
PQARDYLDIYETYGLLSDKLLLGHSIHLEPREIARMAETGSRAVFCPTSNLFLGSGLFDE
CCCHHHHHHHHHHHHHHCCEEECCEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCC
AGLRAAGVVSGIATDVGGGTSYSMLQTLNEGYKILQLRGQKLHPFAAFHWVTRGNALALG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCEEEEE
MAERIGTLDPGSDADLVVLDSRATPAMALRMDRAETLAEELFILQILGDDRAIAQTYVAG
HHHHCCCCCCCCCCCEEEEECCCCCHHHEEHHHHHHHHHHHEEEEEECCCHHHHHHHHCC
KPMLG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]