Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 119386944

GI number: 119386944

Start: 1404840

End: 1405901

Strand: Direct

Name: ada [H]

Synonym: Pden_4239

Alternate gene names: 119386944

Gene position: 1404840-1405901 (Clockwise)

Preceding gene: 119386943

Following gene: 119386945

Centisome position: 81.2

GC content: 73.45

Gene sequence:

>1062_bases
ATGGACCACACCCCGATCCCGACCGAAAGCGACCCGCGCTGGCAGCGGGTGCTGGCGCGCGACGCGGCGGCCGACGGCAG
CTTCGTCTATGCCGTGCGCACCACCGGGGTCTATTGCCGCCCCTCCTGCCCGTCGCGGCGGGCAAAGCCGCAGAACGTGA
CCCTCTACGCCTCGCCCGATCAGGCCGAGGCGGCGGGCTTTCGCCCCTGCCGGCGCTGCAACCCCAGGGGTGCATCGGCC
GCCGAGGCCAATGCCGCGCTGGTCGCCGCCGCCTGTCGCCTGATCGAGCAGGCCGAGACGCCGCCCACCCTTCAGGCGCT
GGCCGAGGCAGCGGGGCTGAGCCCGTTCCACTTTCACCGCCAGTTCAAGGCGATTATCGGGCTGACGCCCCGCGCCTATG
CCGCCGCACATCGCGCCGGCCGTCTGCGCGCCCGCCTGGCCCAAGGCGCCAGCGTCACCGGCGCGATCTACGAGGCGGGC
TTCAACTCGAGCAGCCGCTTCTATGAAGGCGCCGACGGGATGCTGGGCATGACGCCCTCGGCCTGGCGCAAGGGCGGCGC
GGGGGCCGAGATCCGCTTTGCGCTGGGACAATGCGCGCTTGGCGCCATCCTGGTCGCGCAAAGCCGCCGCGGCATCTGCG
CCATCGCGCTTGGCGACGACCCCGAGGCGCTGTTGCGCGACTTCCAGGACCGCTTTCCGGCAGCCACCCTGATCGGCGGC
GACGCGGAATTCGAGGCGCTGGTCGCCCGCATCGTCGGCTTCATCGAGGCGCCGGGCATCGGGCTGGACCTGCCGCTGGA
CATCCGCGGCACCGCCTTCCAGCAGCGCGTCTGGCAGGCCCTGCGCCAGATTCCGCCCGGCACCACCGCCAGCTATGCCG
AGATCGCCCGCCGCATCGGCGAGCCGCAGGCCAGCCGCGCCGTGGCGCAAGCCTGCGCCGCGAACCCGATCGCCGTCGCC
ATCCCCTGCCACCGCGTCGTGCGCAGCGACGGCGCGCTGTCGGGCTATCGCTGGGGCGTGGCGCGCAAGCGCGCCTTGCT
CGACCGCGAGGGGGCGGCATAG

Upstream 100 bases:

>100_bases
CCCCTGAAAGCGCGGATTTGACCGCAAGCGGCGGAGTGCGGCGGTCGTCGGCCGGCCCTAGATATGATAGCAGGAATGAC
GCAGCAGAAAGGTTCCTGCC

Downstream 100 bases:

>100_bases
GCACCGTATTGCCGAGAGTTTCGCTTGGGTATATATCCGGCCGCGATGCTGGCTGCCGGAAAGACCTGCCATGTTCCCGA
TCAGACCCTATCGCAGCACC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MDHTPIPTESDPRWQRVLARDAAADGSFVYAVRTTGVYCRPSCPSRRAKPQNVTLYASPDQAEAAGFRPCRRCNPRGASA
AEANAALVAAACRLIEQAETPPTLQALAEAAGLSPFHFHRQFKAIIGLTPRAYAAAHRAGRLRARLAQGASVTGAIYEAG
FNSSSRFYEGADGMLGMTPSAWRKGGAGAEIRFALGQCALGAILVAQSRRGICAIALGDDPEALLRDFQDRFPAATLIGG
DAEFEALVARIVGFIEAPGIGLDLPLDIRGTAFQQRVWQALRQIPPGTTASYAEIARRIGEPQASRAVAQACAANPIAVA
IPCHRVVRSDGALSGYRWGVARKRALLDREGAA

Sequences:

>Translated_353_residues
MDHTPIPTESDPRWQRVLARDAAADGSFVYAVRTTGVYCRPSCPSRRAKPQNVTLYASPDQAEAAGFRPCRRCNPRGASA
AEANAALVAAACRLIEQAETPPTLQALAEAAGLSPFHFHRQFKAIIGLTPRAYAAAHRAGRLRARLAQGASVTGAIYEAG
FNSSSRFYEGADGMLGMTPSAWRKGGAGAEIRFALGQCALGAILVAQSRRGICAIALGDDPEALLRDFQDRFPAATLIGG
DAEFEALVARIVGFIEAPGIGLDLPLDIRGTAFQQRVWQALRQIPPGTTASYAEIARRIGEPQASRAVAQACAANPIAVA
IPCHRVVRSDGALSGYRWGVARKRALLDREGAA
>Mature_353_residues
MDHTPIPTESDPRWQRVLARDAAADGSFVYAVRTTGVYCRPSCPSRRAKPQNVTLYASPDQAEAAGFRPCRRCNPRGASA
AEANAALVAAACRLIEQAETPPTLQALAEAAGLSPFHFHRQFKAIIGLTPRAYAAAHRAGRLRARLAQGASVTGAIYEAG
FNSSSRFYEGADGMLGMTPSAWRKGGAGAEIRFALGQCALGAILVAQSRRGICAIALGDDPEALLRDFQDRFPAATLIGG
DAEFEALVARIVGFIEAPGIGLDLPLDIRGTAFQQRVWQALRQIPPGTTASYAEIARRIGEPQASRAVAQACAANPIAVA
IPCHRVVRSDGALSGYRWGVARKRALLDREGAA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=124, Percent_Identity=33.8709677419355, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788542, Length=341, Percent_Identity=56.3049853372434, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=46.5909090909091, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=133, Percent_Identity=42.8571428571429, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=133, Percent_Identity=42.8571428571429, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=44.4444444444444, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137554, Length=164, Percent_Identity=34.7560975609756, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 37459; Mature: 37459

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTPIPTESDPRWQRVLARDAAADGSFVYAVRTTGVYCRPSCPSRRAKPQNVTLYASPD
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEECCC
QAEAAGFRPCRRCNPRGASAAEANAALVAAACRLIEQAETPPTLQALAEAAGLSPFHFHR
CHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHH
QFKAIIGLTPRAYAAAHRAGRLRARLAQGASVTGAIYEAGFNSSSRFYEGADGMLGMTPS
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHH
AWRKGGAGAEIRFALGQCALGAILVAQSRRGICAIALGDDPEALLRDFQDRFPAATLIGG
HHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCEEEEECC
DAEFEALVARIVGFIEAPGIGLDLPLDIRGTAFQQRVWQALRQIPPGTTASYAEIARRIG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
EPQASRAVAQACAANPIAVAIPCHRVVRSDGALSGYRWGVARKRALLDREGAA
CCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDHTPIPTESDPRWQRVLARDAAADGSFVYAVRTTGVYCRPSCPSRRAKPQNVTLYASPD
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEECCC
QAEAAGFRPCRRCNPRGASAAEANAALVAAACRLIEQAETPPTLQALAEAAGLSPFHFHR
CHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHH
QFKAIIGLTPRAYAAAHRAGRLRARLAQGASVTGAIYEAGFNSSSRFYEGADGMLGMTPS
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHH
AWRKGGAGAEIRFALGQCALGAILVAQSRRGICAIALGDDPEALLRDFQDRFPAATLIGG
HHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCEEEEECC
DAEFEALVARIVGFIEAPGIGLDLPLDIRGTAFQQRVWQALRQIPPGTTASYAEIARRIG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
EPQASRAVAQACAANPIAVAIPCHRVVRSDGALSGYRWGVARKRALLDREGAA
CCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]