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The map label for this gene is braD [H]

Identifier: 119386725

GI number: 119386725

Start: 1165346

End: 1167265

Strand: Direct

Name: braD [H]

Synonym: Pden_4018

Alternate gene names: 119386725

Gene position: 1165346-1167265 (Clockwise)

Preceding gene: 119386724

Following gene: 119386726

Centisome position: 67.36

GC content: 70.0

Gene sequence:

>1920_bases
ATGCCCATGACCTGCCGCCTGATCGCCTTGCTGCTGAACCTGTTCCTGGCCTTGCCCGCCGCCGCCAATCCGGCGCTGGA
AGCGGTCATCGCCGACAACCTGGACCAGATCGAGCGCCCCTCGCGCCGCACGGTCGAGCCGGTGGTGGCCCGGATCGCCG
CCACCGGACCCGAGGGGATGGCGCTGCTGTCGGCATGGGCGGGCCGCTCGCTTGGCCTGGCCGACGGGCGGCTGGTGATC
GTCACGGGCGACGGCGCCACGGACGCGGTGACCGGGCAGGCCGTCACGGGCGCGGACCCGAAGATGCTGCGCCCCAATGC
CGGGGTGCGCGGCGTGATCGAATCGGCGCTGGTCGCGGGCGAGATTGCCAGCCCCGACCGGGCGCGCCGCGCCGCCGCGC
TGGCCAGCATCGCCCGCGACGGCACCGCCGAGCATCTGGCCGCCCTGCGCGCCGCCGCGCCCGAGCCGGACCCCGCTCTG
GCCGCCCGCCGCGCCCGGATCGAAAAGCTGCTGACCATCCGCTTCGACGCCGACCCCGCCGCGCGGGTGACCGCCATTCA
GGACATGGCGGGCGATGTCGGCCTTGATTTCCGCGCCGCGCTGAACCCTTTGCTCGCGACCCGGCGCGTGGCCTCGGCCG
ATGAGCCGCAGGGCGCCAACATCGCCCGTGAGTTGCGCGTGGGGTCGGACGATTTCCCGCGCGAGGCCGCCATCGCCCTG
CTGACGGCGCAGGGGCTGGTCCAGCCGCGCCTGACTGCTGCCGACCAGCGCAATGCACTGGCCGCGCATATCGTCGATGG
CAGGGTAGGCGGCGTTTCCGTCGCCGAACTGTCCGACCCGCAAGCCCGCGACCGCGCCTATGAGGCGCTGGAGGGGGCGG
GAACCGTCCCGCCCGCCGCCACCGAGGCCGAGACCGAGGCGGCGCTGGCAGGCATCCGCTTCTTCGATGTCTATGCAGAG
CCTGAGGCGGCCGTGACCGACGCCGCCCGCGCCGCGCAAAGGGGTGCGGAATGGCGGCTGGGGGCGATGAAGGGGATCGA
CCTTGGGCTGGACGCGCTGTCTCTTGCCTCGATCTACTTCCTGGCCGCAATCGGGCTGGCGATCACCTTCGGGGTGATGC
GCGTCATCAACATGGCGCATGGCGAATTCATCATGATGGGCGCCTATACCGGCTATGTCGCGCAGACCTTGATCGCGGAT
CGCACCCTGTCGCTGGTGGCGGCGCTGCCGCTGGCCTTCGCGGTGACCTTTGCCGCAGGCGTGGTGCTTTACCGGCTGGT
GATCCGGCACCTGGCGCATCGGCCGCTGGAGACGCTGCTTGCGACCTTCGGCGTCTCGATCGCGCTGCAGCAGCTTGCCA
AGAACATCTTCGGCACGCAGGCCCGGCCGCTGACCGCACCCGCATGGCTGGAAGGCGCGATCACGGTGAACGACGTGGTC
TCGATCTCGACCATCCGGGTGGCGATCTTCGTGCTGGCGCTGCTGTTCCTGGGCCTGTTCCTGTTCATCATGAAGCGCAC
GAGGCTGGGACTGGAGGTCCGCGCGGTGACGCAGAACCCCGGCATGGCCGCCAGCATGGGGATCAACCCGGACCGCATCG
CCATGATGACCTTTGGCCTCGGCTCGGGCATCGCGGGGATCGCGGGCGTCGCCATCGGCCTTTTCGCCCAGGTCACCTCG
GAACTGGGCCAGCAATACATCGTGCAAAGCTTCATGACGGTTGTGGTCGGCGGCGTCGGCAACATCTGGGGCACGCTGGC
GGGCGCCGGGCTGATCGGGGTGCTGTCCAAGGTGATCGAATCCTTCAATCCCTCGAACACGCTGGCGGCGCAGACCTTCA
TGATCCTGTTCATCGTGATCTTCATCCAATTCCGGCCGCGCGGCATCATCCCGCAGCGCGGCCGTGCGGCGGAGGCGTGA

Upstream 100 bases:

>100_bases
CATGTTCAACACGCAGACCAACAGCTGCGTGCAGGTCCAGTCGAACTACTGACCGGCTTTCGGGGGCGGTGCAGCCGCCC
TCTTTCGCCTCAAGACGGAA

Downstream 100 bases:

>100_bases
CATGACCCGGCAACCCTTCATCCTGCGCAATCCCTCGGTCCTGGTCGTCCTGGCATTGCTGGCCGCCTTTACCCTTGCCG
TCACGCTGATGAGCACGGCC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MPMTCRLIALLLNLFLALPAAANPALEAVIADNLDQIERPSRRTVEPVVARIAATGPEGMALLSAWAGRSLGLADGRLVI
VTGDGATDAVTGQAVTGADPKMLRPNAGVRGVIESALVAGEIASPDRARRAAALASIARDGTAEHLAALRAAAPEPDPAL
AARRARIEKLLTIRFDADPAARVTAIQDMAGDVGLDFRAALNPLLATRRVASADEPQGANIARELRVGSDDFPREAAIAL
LTAQGLVQPRLTAADQRNALAAHIVDGRVGGVSVAELSDPQARDRAYEALEGAGTVPPAATEAETEAALAGIRFFDVYAE
PEAAVTDAARAAQRGAEWRLGAMKGIDLGLDALSLASIYFLAAIGLAITFGVMRVINMAHGEFIMMGAYTGYVAQTLIAD
RTLSLVAALPLAFAVTFAAGVVLYRLVIRHLAHRPLETLLATFGVSIALQQLAKNIFGTQARPLTAPAWLEGAITVNDVV
SISTIRVAIFVLALLFLGLFLFIMKRTRLGLEVRAVTQNPGMAASMGINPDRIAMMTFGLGSGIAGIAGVAIGLFAQVTS
ELGQQYIVQSFMTVVVGGVGNIWGTLAGAGLIGVLSKVIESFNPSNTLAAQTFMILFIVIFIQFRPRGIIPQRGRAAEA

Sequences:

>Translated_639_residues
MPMTCRLIALLLNLFLALPAAANPALEAVIADNLDQIERPSRRTVEPVVARIAATGPEGMALLSAWAGRSLGLADGRLVI
VTGDGATDAVTGQAVTGADPKMLRPNAGVRGVIESALVAGEIASPDRARRAAALASIARDGTAEHLAALRAAAPEPDPAL
AARRARIEKLLTIRFDADPAARVTAIQDMAGDVGLDFRAALNPLLATRRVASADEPQGANIARELRVGSDDFPREAAIAL
LTAQGLVQPRLTAADQRNALAAHIVDGRVGGVSVAELSDPQARDRAYEALEGAGTVPPAATEAETEAALAGIRFFDVYAE
PEAAVTDAARAAQRGAEWRLGAMKGIDLGLDALSLASIYFLAAIGLAITFGVMRVINMAHGEFIMMGAYTGYVAQTLIAD
RTLSLVAALPLAFAVTFAAGVVLYRLVIRHLAHRPLETLLATFGVSIALQQLAKNIFGTQARPLTAPAWLEGAITVNDVV
SISTIRVAIFVLALLFLGLFLFIMKRTRLGLEVRAVTQNPGMAASMGINPDRIAMMTFGLGSGIAGIAGVAIGLFAQVTS
ELGQQYIVQSFMTVVVGGVGNIWGTLAGAGLIGVLSKVIESFNPSNTLAAQTFMILFIVIFIQFRPRGIIPQRGRAAEA
>Mature_638_residues
PMTCRLIALLLNLFLALPAAANPALEAVIADNLDQIERPSRRTVEPVVARIAATGPEGMALLSAWAGRSLGLADGRLVIV
TGDGATDAVTGQAVTGADPKMLRPNAGVRGVIESALVAGEIASPDRARRAAALASIARDGTAEHLAALRAAAPEPDPALA
ARRARIEKLLTIRFDADPAARVTAIQDMAGDVGLDFRAALNPLLATRRVASADEPQGANIARELRVGSDDFPREAAIALL
TAQGLVQPRLTAADQRNALAAHIVDGRVGGVSVAELSDPQARDRAYEALEGAGTVPPAATEAETEAALAGIRFFDVYAEP
EAAVTDAARAAQRGAEWRLGAMKGIDLGLDALSLASIYFLAAIGLAITFGVMRVINMAHGEFIMMGAYTGYVAQTLIADR
TLSLVAALPLAFAVTFAAGVVLYRLVIRHLAHRPLETLLATFGVSIALQQLAKNIFGTQARPLTAPAWLEGAITVNDVVS
ISTIRVAIFVLALLFLGLFLFIMKRTRLGLEVRAVTQNPGMAASMGINPDRIAMMTFGLGSGIAGIAGVAIGLFAQVTSE
LGQQYIVQSFMTVVVGGVGNIWGTLAGAGLIGVLSKVIESFNPSNTLAAQTFMILFIVIFIQFRPRGIIPQRGRAAEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=296, Percent_Identity=29.0540540540541, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 66815; Mature: 66683

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTCRLIALLLNLFLALPAAANPALEAVIADNLDQIERPSRRTVEPVVARIAATGPEGM
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCHH
ALLSAWAGRSLGLADGRLVIVTGDGATDAVTGQAVTGADPKMLRPNAGVRGVIESALVAG
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHC
EIASPDRARRAAALASIARDGTAEHLAALRAAAPEPDPALAARRARIEKLLTIRFDADPA
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCH
ARVTAIQDMAGDVGLDFRAALNPLLATRRVASADEPQGANIARELRVGSDDFPREAAIAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
LTAQGLVQPRLTAADQRNALAAHIVDGRVGGVSVAELSDPQARDRAYEALEGAGTVPPAA
HHHHHCCCCCHHHHHCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC
TEAETEAALAGIRFFDVYAEPEAAVTDAARAAQRGAEWRLGAMKGIDLGLDALSLASIYF
CCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHH
LAAIGLAITFGVMRVINMAHGEFIMMGAYTGYVAQTLIADRTLSLVAALPLAFAVTFAAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVLYRLVIRHLAHRPLETLLATFGVSIALQQLAKNIFGTQARPLTAPAWLEGAITVNDVV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEHHHHH
SISTIRVAIFVLALLFLGLFLFIMKRTRLGLEVRAVTQNPGMAASMGINPDRIAMMTFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEEECC
GSGIAGIAGVAIGLFAQVTSELGQQYIVQSFMTVVVGGVGNIWGTLAGAGLIGVLSKVIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SFNPSNTLAAQTFMILFIVIFIQFRPRGIIPQRGRAAEA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMTCRLIALLLNLFLALPAAANPALEAVIADNLDQIERPSRRTVEPVVARIAATGPEGM
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCHH
ALLSAWAGRSLGLADGRLVIVTGDGATDAVTGQAVTGADPKMLRPNAGVRGVIESALVAG
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHC
EIASPDRARRAAALASIARDGTAEHLAALRAAAPEPDPALAARRARIEKLLTIRFDADPA
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCH
ARVTAIQDMAGDVGLDFRAALNPLLATRRVASADEPQGANIARELRVGSDDFPREAAIAL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
LTAQGLVQPRLTAADQRNALAAHIVDGRVGGVSVAELSDPQARDRAYEALEGAGTVPPAA
HHHHHCCCCCHHHHHCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCC
TEAETEAALAGIRFFDVYAEPEAAVTDAARAAQRGAEWRLGAMKGIDLGLDALSLASIYF
CCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHH
LAAIGLAITFGVMRVINMAHGEFIMMGAYTGYVAQTLIADRTLSLVAALPLAFAVTFAAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVLYRLVIRHLAHRPLETLLATFGVSIALQQLAKNIFGTQARPLTAPAWLEGAITVNDVV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEHHHHH
SISTIRVAIFVLALLFLGLFLFIMKRTRLGLEVRAVTQNPGMAASMGINPDRIAMMTFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEEECC
GSGIAGIAGVAIGLFAQVTSELGQQYIVQSFMTVVVGGVGNIWGTLAGAGLIGVLSKVIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SFNPSNTLAAQTFMILFIVIFIQFRPRGIIPQRGRAAEA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]