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The map label for this gene is ytcB [H]

Identifier: 119386264

GI number: 119386264

Start: 701536

End: 702444

Strand: Direct

Name: ytcB [H]

Synonym: Pden_3553

Alternate gene names: 119386264

Gene position: 701536-702444 (Clockwise)

Preceding gene: 119386263

Following gene: 119386270

Centisome position: 40.55

GC content: 69.09

Gene sequence:

>909_bases
ATGATCGCGCCCGGTTCCTTTGTCGGGGTCACTGGCGCCTCGGGATTCATCGGGCGCCATCTTTGTGCCGATTTGCGCGC
GGCCGGCTTGCGCCCCGTCGCCATCGGCCGGGGGCCGGAGGCCGAGCGGCAGACCGACTACAGTCCCGAAAGCCTGCGTG
CGGCACTGGCGGGCTGTGCCGCCGTGGTGCATCTGGCTGGCCGGCGCATGACGCGCGAGGACGCGCCGATGGAACTTGCT
CCGTTCTTGGGTCCCAATGTCGAGGCGACCGGCCATCTCGCCCGCGCGGCGCAGGCAGAGGGGGTGGAGCGGATCGTCTT
CGCCTCGACCATCGCGGTCTATTCCGCCGCCAGCCCCGCGCCCTGGCGCGAGGACGGACCCGTCCATCCGGTCAATGCCT
ATGCCCTCTCGAAGCTGATGGCGGAGCACTATCTCGAGATGCTGGCCCGCGCCCGCCAGGCGCCGCCCGCGCTCTCGTTG
CGCTTTGCCGCGGTCTATGGCCATGGCGAGAAGGGCACGCCCGCGCTGATGAAGTTCGTGAACCAGGCGGCAGCGGGGGA
AACCATCACGCTGAGCGGCAACCCGGATTACACGATCGACCAGCTTTACGTCACCGATGCGACGGCTGCGATCCTCGCCG
CGCTCAGTGTGTCCGCACCGCTCTCGGGGGCCTGCAATATCGGCGGTGGCCGCGCCTGGACCGTGGCCGAGATCGCCCGG
ACTGCGAATGCGGTCTTTGGCAATGACGGCAATCTCGACGACAGCGCCACCACGCCGGGGCAGGCGCATCAGGCGGTGAT
GGACCTTGCCCAGGCAAACCGGATTCTTGGCTGGCAGCCCGCCCACGACTTGCAGGCCGGCCTTGCCGACTTGCGGCGGC
GCAGGGAAGAAGGTGGCCGCGGGGAATGA

Upstream 100 bases:

>100_bases
CCGATGCCCTTTATCACGAATACCGCTACGCCGCCGGCGAGGATGTGCGGGCAGTCAACGGCACGCTTGAAGTGGTGGGC
TATTACGTGCGGGCAATGTC

Downstream 100 bases:

>100_bases
TCCCGCGGCCCAGCCAGCCTTTCAGACGCGTGTTCCCTCCGCGCTTTCGAGAAGCAGTTCAATATTCAGCAGATTGCGGG
CAATGGACTGATTGAACACG

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MIAPGSFVGVTGASGFIGRHLCADLRAAGLRPVAIGRGPEAERQTDYSPESLRAALAGCAAVVHLAGRRMTREDAPMELA
PFLGPNVEATGHLARAAQAEGVERIVFASTIAVYSAASPAPWREDGPVHPVNAYALSKLMAEHYLEMLARARQAPPALSL
RFAAVYGHGEKGTPALMKFVNQAAAGETITLSGNPDYTIDQLYVTDATAAILAALSVSAPLSGACNIGGGRAWTVAEIAR
TANAVFGNDGNLDDSATTPGQAHQAVMDLAQANRILGWQPAHDLQAGLADLRRRREEGGRGE

Sequences:

>Translated_302_residues
MIAPGSFVGVTGASGFIGRHLCADLRAAGLRPVAIGRGPEAERQTDYSPESLRAALAGCAAVVHLAGRRMTREDAPMELA
PFLGPNVEATGHLARAAQAEGVERIVFASTIAVYSAASPAPWREDGPVHPVNAYALSKLMAEHYLEMLARARQAPPALSL
RFAAVYGHGEKGTPALMKFVNQAAAGETITLSGNPDYTIDQLYVTDATAAILAALSVSAPLSGACNIGGGRAWTVAEIAR
TANAVFGNDGNLDDSATTPGQAHQAVMDLAQANRILGWQPAHDLQAGLADLRRRREEGGRGE
>Mature_302_residues
MIAPGSFVGVTGASGFIGRHLCADLRAAGLRPVAIGRGPEAERQTDYSPESLRAALAGCAAVVHLAGRRMTREDAPMELA
PFLGPNVEATGHLARAAQAEGVERIVFASTIAVYSAASPAPWREDGPVHPVNAYALSKLMAEHYLEMLARARQAPPALSL
RFAAVYGHGEKGTPALMKFVNQAAAGETITLSGNPDYTIDQLYVTDATAAILAALSVSAPLSGACNIGGGRAWTVAEIAR
TANAVFGNDGNLDDSATTPGQAHQAVMDLAQANRILGWQPAHDLQAGLADLRRRREEGGRGE

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=341, Percent_Identity=26.9794721407625, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=341, Percent_Identity=26.9794721407625, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=341, Percent_Identity=26.9794721407625, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786974, Length=315, Percent_Identity=25.0793650793651, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 31510; Mature: 31510

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPGSFVGVTGASGFIGRHLCADLRAAGLRPVAIGRGPEAERQTDYSPESLRAALAGCA
CCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHH
AVVHLAGRRMTREDAPMELAPFLGPNVEATGHLARAAQAEGVERIVFASTIAVYSAASPA
HHHHHHHHHCCCCCCCCEEHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
PWREDGPVHPVNAYALSKLMAEHYLEMLARARQAPPALSLRFAAVYGHGEKGTPALMKFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHH
NQAAAGETITLSGNPDYTIDQLYVTDATAAILAALSVSAPLSGACNIGGGRAWTVAEIAR
HHHHCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
TANAVFGNDGNLDDSATTPGQAHQAVMDLAQANRILGWQPAHDLQAGLADLRRRREEGGR
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GE
CC
>Mature Secondary Structure
MIAPGSFVGVTGASGFIGRHLCADLRAAGLRPVAIGRGPEAERQTDYSPESLRAALAGCA
CCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHH
AVVHLAGRRMTREDAPMELAPFLGPNVEATGHLARAAQAEGVERIVFASTIAVYSAASPA
HHHHHHHHHCCCCCCCCEEHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
PWREDGPVHPVNAYALSKLMAEHYLEMLARARQAPPALSLRFAAVYGHGEKGTPALMKFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHH
NQAAAGETITLSGNPDYTIDQLYVTDATAAILAALSVSAPLSGACNIGGGRAWTVAEIAR
HHHHCCCEEEECCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
TANAVFGNDGNLDDSATTPGQAHQAVMDLAQANRILGWQPAHDLQAGLADLRRRREEGGR
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]