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The map label for this gene is garR [H]

Identifier: 119385976

GI number: 119385976

Start: 419378

End: 420307

Strand: Direct

Name: garR [H]

Synonym: Pden_3256

Alternate gene names: 119385976

Gene position: 419378-420307 (Clockwise)

Preceding gene: 119385975

Following gene: 119385977

Centisome position: 24.24

GC content: 67.85

Gene sequence:

>930_bases
ATGAAAATTGGCTTTATCGGCCTGGGCAAGATGGGGCGCGGCATGGCCGCCAACCTGCAAACGGGCGGCGCCGCTTTGGT
AGTGGCCGACCTGTCCCGCACCGCCGCCGAGGAGTTCACCGTGCGGGGCGCCACCTGGGCCGAGACCCCGGCGGACCTTG
CGCGGGATTGCGGGATCGTCTTCACCTCGTTGCCGACCCCGGCGGATGTGCGCAACGCGGGCTTCGGCCCGCGCGGGCTG
GTCGAAGGCATGGCACCGGGTACGGTCTGGCTCGACCTGTCGACCAACAGTGTCGACGTGGTGCGCCAGATCCATGCCGA
ACTTGCGCCGAAAGGCATCCATTTCATCGATGCCCCGGTCAGCGGCGGGCCCGCCGGTGCCGCCAGCGGCCAGTTGGCGA
TCTGGTGCGGCGGCGAACGCGAGGTTTACGACCGCTGCTTGCCCTGGCTGGAGAAGATGGCCGACCAGCCGCGCTATATC
GGCGACATCGGCGCGGGCACCATCGCCAAGCTGGTCAACAACATGGCCAGCACCGCGATCATCTCGGTGCTGGCCGAGGC
GCTGAGCATGGGCGTCAAGGCGGGGTTGGAACCCGGCCCACTGTGGGAGGCGATCCGCACCGGCGCCGCCGGGCGGATGC
GGATGTACGACAACATCGGTCGCCGCTTCATGCAGGCGAAATACGATCCTCCCAGCTTCGCGCTGCGGCTGATCCACAAG
GACGCCTCGCTGGCCTTGCAGGTCGGGCGCGATTTCGGCGTGCCGATGCGACTCTGCACGCTGGTAGAGAGCGACATCCG
CGAGGCGCTGAACCGGGGCTGGGGCGAGCGCGACAGCCAGTCCTATCTGGCGCTGCAACAGGAACGCGCCGGGATCGAAC
CCTTCCGACTGAGTGCCGAGGCAGTCGCCCGCATTTACGCGGAAAGCTGA

Upstream 100 bases:

>100_bases
TCGGACATCGCTTCCATGCCCGTCCGGACGGCCTTGATTTCGGAACATGACACCCCCGCCGGCAAGCCAGCGCCGGGCGG
CATCGGGAAAGGACTGTCTG

Downstream 100 bases:

>100_bases
CCGGCTTTCTGGTAATCCTACAGGAGAACACCCTATGGACAGAATCGAAACAGGCCGGCAGGTCCTCCGGGAGTTGATGG
GCGAGGATTTCCTCGTCCAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKIGFIGLGKMGRGMAANLQTGGAALVVADLSRTAAEEFTVRGATWAETPADLARDCGIVFTSLPTPADVRNAGFGPRGL
VEGMAPGTVWLDLSTNSVDVVRQIHAELAPKGIHFIDAPVSGGPAGAASGQLAIWCGGEREVYDRCLPWLEKMADQPRYI
GDIGAGTIAKLVNNMASTAIISVLAEALSMGVKAGLEPGPLWEAIRTGAAGRMRMYDNIGRRFMQAKYDPPSFALRLIHK
DASLALQVGRDFGVPMRLCTLVESDIREALNRGWGERDSQSYLALQQERAGIEPFRLSAEAVARIYAES

Sequences:

>Translated_309_residues
MKIGFIGLGKMGRGMAANLQTGGAALVVADLSRTAAEEFTVRGATWAETPADLARDCGIVFTSLPTPADVRNAGFGPRGL
VEGMAPGTVWLDLSTNSVDVVRQIHAELAPKGIHFIDAPVSGGPAGAASGQLAIWCGGEREVYDRCLPWLEKMADQPRYI
GDIGAGTIAKLVNNMASTAIISVLAEALSMGVKAGLEPGPLWEAIRTGAAGRMRMYDNIGRRFMQAKYDPPSFALRLIHK
DASLALQVGRDFGVPMRLCTLVESDIREALNRGWGERDSQSYLALQQERAGIEPFRLSAEAVARIYAES
>Mature_309_residues
MKIGFIGLGKMGRGMAANLQTGGAALVVADLSRTAAEEFTVRGATWAETPADLARDCGIVFTSLPTPADVRNAGFGPRGL
VEGMAPGTVWLDLSTNSVDVVRQIHAELAPKGIHFIDAPVSGGPAGAASGQLAIWCGGEREVYDRCLPWLEKMADQPRYI
GDIGAGTIAKLVNNMASTAIISVLAEALSMGVKAGLEPGPLWEAIRTGAAGRMRMYDNIGRRFMQAKYDPPSFALRLIHK
DASLALQVGRDFGVPMRLCTLVESDIREALNRGWGERDSQSYLALQQERAGIEPFRLSAEAVARIYAES

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=254, Percent_Identity=28.740157480315, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI23308751, Length=302, Percent_Identity=29.8013245033113, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI145693186, Length=296, Percent_Identity=34.1216216216216, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790315, Length=303, Percent_Identity=31.3531353135314, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786719, Length=250, Percent_Identity=32.4, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=27.1777003484321, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788341, Length=323, Percent_Identity=27.8637770897833, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557316, Length=280, Percent_Identity=30.3571428571429, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24655230, Length=304, Percent_Identity=31.9078947368421, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922568, Length=304, Percent_Identity=31.9078947368421, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=21.6312056737589, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 33001; Mature: 33001

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFIGLGKMGRGMAANLQTGGAALVVADLSRTAAEEFTVRGATWAETPADLARDCGIV
CEEEEEECCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHHHHHHHCCEE
FTSLPTPADVRNAGFGPRGLVEGMAPGTVWLDLSTNSVDVVRQIHAELAPKGIHFIDAPV
EECCCCCCHHHCCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCC
SGGPAGAASGQLAIWCGGEREVYDRCLPWLEKMADQPRYIGDIGAGTIAKLVNNMASTAI
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHH
ISVLAEALSMGVKAGLEPGPLWEAIRTGAAGRMRMYDNIGRRFMQAKYDPPSFALRLIHK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
DASLALQVGRDFGVPMRLCTLVESDIREALNRGWGERDSQSYLALQQERAGIEPFRLSAE
CHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHEECHH
AVARIYAES
HHHHHHCCC
>Mature Secondary Structure
MKIGFIGLGKMGRGMAANLQTGGAALVVADLSRTAAEEFTVRGATWAETPADLARDCGIV
CEEEEEECCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHHHHHHHCCEE
FTSLPTPADVRNAGFGPRGLVEGMAPGTVWLDLSTNSVDVVRQIHAELAPKGIHFIDAPV
EECCCCCCHHHCCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCC
SGGPAGAASGQLAIWCGGEREVYDRCLPWLEKMADQPRYIGDIGAGTIAKLVNNMASTAI
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHH
ISVLAEALSMGVKAGLEPGPLWEAIRTGAAGRMRMYDNIGRRFMQAKYDPPSFALRLIHK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
DASLALQVGRDFGVPMRLCTLVESDIREALNRGWGERDSQSYLALQQERAGIEPFRLSAE
CHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHEECHH
AVARIYAES
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]