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The map label for this gene is hphIAM [H]

Identifier: 119385904

GI number: 119385904

Start: 348795

End: 350282

Strand: Direct

Name: hphIAM [H]

Synonym: Pden_3182

Alternate gene names: 119385904

Gene position: 348795-350282 (Clockwise)

Preceding gene: 119385903

Following gene: 119385905

Centisome position: 20.16

GC content: 67.74

Gene sequence:

>1488_bases
ATGAGCGCCCGTCGCACCCACCTTTCCCTGCCGTTGCGCGTACTCGACCTCTTCAGCGGCGCCGCGGGCGGCTGGTCGCT
CGGCCTGCATCGGGCGGGCTTTGTCACCGTCGCCGCCTGCGAGATCGTCGCATGGCGGCGCATCCTCTATTCGGAGAATT
TTCCGCATGTCAGACTCCACGAGGACGTCCGAACCCTCACGGCAGCTCGACTTGTTTCCGACCTCGGTGGCCTGCCGGAG
ATCATCGTCGGCTCGCCGCCATGCCAGGACATCTCGAGCGCCAACACCAAGGGCAAGGGGATCGAGGGCGAACGCTCGGG
CCTCTACCTCGAGGCCGTGCGGCTGGTTGGAGAGTGCCGCCCTCGCTGGTTCGCTTTTGAGAACAGCTCTGTGCTCAGAA
CTCGCGGCGCCGACCGGCTGCTCGATGAGCTGGAAGCGCTCGGCTACGCCTGCTGGCCATGCGTGGTGGGTGCTGCGGAT
ATCGGCGCCAACCATGTCCGCAAGCGGTCCTGGCTCATCGGCTGCGATCCCGAACAGCTTGCCGACACCCGTGTCGCAAT
CCCAGCAGGGTGGATTGCGGCTGGAGGGCGGATCGGGGGCGCGGAAGGCGATGAAGGACTCGGGTCTTTCCGATCTGTTC
CGCAACCGACCCTTGCCGACGCCGATGGCCTCGGACGGGATGAAGGACGGCGCCGGCGGCGGCGCGGGATCGACATATCC
GCTGCGGATGATCCTGGCGACGCCGCGGAAGTCCGACGCGCATCGCGGTGGCCGGGGCGATCTGCTGAGCCAGTTGCAGG
GCCACAACAGCCACCATGCGGGCATGATGGGCACGCCGACGGCGAATGCGGCACCGCGGGGCAGCGTGTTGCGCAAGCAC
AAGGGCCGGATGACCTCGGATCAGGACCGTTTCGACCGCAGCCCGACGATCGGCGAAGCGCTGCATTTCGACAGGCGGGT
GCCGGAAATCCTGCCGACGCCGACGAAGCGGGACAGCCGGATGGACGGCTGGAGCCCGGCCTACGACCGTCGGAAGTCGC
CGACCATGGATGCGGTGATGGACGGGGCGATGACGGATCGGGCGCCGGACAAATGGGCCGGGGCGCGGGCGCTGGCGGCG
ATCTTGCGGAGCCATGGCCTGACTGGAACGGCGGCATTGCCCATCACATACGGGTGGATGATGGGATTTCCGCCTGGCTG
GCTCGCACGCACCTTGCGCTCGGCAGCCGCAAAGGGACGTCTGCGGCGAGCCTGATTGTCGAAGCCTTCGGCGATGCGGT
GGTGCCGCAGATCCCCGAGGCCATCGGCCGCGCCATTCTGCGCAGCGAGGCCGCGCTGAACGCGGTGCTCGGTGGTGCTC
CGGCGAACGATGCCGCTAACGACGACGGTCAATCCATCGACGCTGGCCGAGGGAATGCCGCGCCCAACGACAATGCTGTC
GATGAAGCTGCCAGTGGCAGCCCGACGGACCACGGGGGTGCGCCATGA

Upstream 100 bases:

>100_bases
ACTGGCGGCATGTCCACAACCGTATGGTCGCGGGCGAGCCGTTCCGGACCTACACCAGGGCCCAGCATGCCGCATGGCTG
AAGCGCCGAAGGGTCGGCGT

Downstream 100 bases:

>100_bases
TCCGCAGAACGCGCACCACCTATCTCGCCGCAACTGCTGCCGCCGTGATGGTGATCGGTGCTGTTTCCGTCACCGACATC
ACGCCGCGGCTGATCTGGAA

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSARRTHLSLPLRVLDLFSGAAGGWSLGLHRAGFVTVAACEIVAWRRILYSENFPHVRLHEDVRTLTAARLVSDLGGLPE
IIVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSVLRTRGADRLLDELEALGYACWPCVVGAAD
IGANHVRKRSWLIGCDPEQLADTRVAIPAGWIAAGGRIGGAEGDEGLGSFRSVPQPTLADADGLGRDEGRRRRRRGIDIS
AADDPGDAAEVRRASRWPGRSAEPVAGPQQPPCGHDGHADGECGTAGQRVAQAQGPDDLGSGPFRPQPDDRRSAAFRQAG
AGNPADADEAGQPDGRLEPGLRPSEVADHGCGDGRGDDGSGAGQMGRGAGAGGDLAEPWPDWNGGIAHHIRVDDGISAWL
ARTHLALGSRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPANDAANDDGQSIDAGRGNAAPNDNAV
DEAASGSPTDHGGAP

Sequences:

>Translated_495_residues
MSARRTHLSLPLRVLDLFSGAAGGWSLGLHRAGFVTVAACEIVAWRRILYSENFPHVRLHEDVRTLTAARLVSDLGGLPE
IIVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSVLRTRGADRLLDELEALGYACWPCVVGAAD
IGANHVRKRSWLIGCDPEQLADTRVAIPAGWIAAGGRIGGAEGDEGLGSFRSVPQPTLADADGLGRDEGRRRRRRGIDIS
AADDPGDAAEVRRASRWPGRSAEPVAGPQQPPCGHDGHADGECGTAGQRVAQAQGPDDLGSGPFRPQPDDRRSAAFRQAG
AGNPADADEAGQPDGRLEPGLRPSEVADHGCGDGRGDDGSGAGQMGRGAGAGGDLAEPWPDWNGGIAHHIRVDDGISAWL
ARTHLALGSRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPANDAANDDGQSIDAGRGNAAPNDNAV
DEAASGSPTDHGGAP
>Mature_494_residues
SARRTHLSLPLRVLDLFSGAAGGWSLGLHRAGFVTVAACEIVAWRRILYSENFPHVRLHEDVRTLTAARLVSDLGGLPEI
IVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSVLRTRGADRLLDELEALGYACWPCVVGAADI
GANHVRKRSWLIGCDPEQLADTRVAIPAGWIAAGGRIGGAEGDEGLGSFRSVPQPTLADADGLGRDEGRRRRRRGIDISA
ADDPGDAAEVRRASRWPGRSAEPVAGPQQPPCGHDGHADGECGTAGQRVAQAQGPDDLGSGPFRPQPDDRRSAAFRQAGA
GNPADADEAGQPDGRLEPGLRPSEVADHGCGDGRGDDGSGAGQMGRGAGAGGDLAEPWPDWNGGIAHHIRVDDGISAWLA
RTHLALGSRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPANDAANDDGQSIDAGRGNAAPNDNAVD
EAASGSPTDHGGAP

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 51263; Mature: 51132

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARRTHLSLPLRVLDLFSGAAGGWSLGLHRAGFVTVAACEIVAWRRILYSENFPHVRLH
CCCCCCHHCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEH
EDVRTLTAARLVSDLGGLPEIIVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECR
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PRWFAFENSSVLRTRGADRLLDELEALGYACWPCVVGAADIGANHVRKRSWLIGCDPEQL
CCEEEECCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHCCEEEECCHHHH
ADTRVAIPAGWIAAGGRIGGAEGDEGLGSFRSVPQPTLADADGLGRDEGRRRRRRGIDIS
CCCEEECCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
AADDPGDAAEVRRASRWPGRSAEPVAGPQQPPCGHDGHADGECGTAGQRVAQAQGPDDLG
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
SGPFRPQPDDRRSAAFRQAGAGNPADADEAGQPDGRLEPGLRPSEVADHGCGDGRGDDGS
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
GAGQMGRGAGAGGDLAEPWPDWNGGIAHHIRVDDGISAWLARTHLALGSRKGTSAASLIV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
EAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPANDAANDDGQSIDAGRGNAAPNDNAV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DEAASGSPTDHGGAP
HHHCCCCCCCCCCCC
>Mature Secondary Structure 
SARRTHLSLPLRVLDLFSGAAGGWSLGLHRAGFVTVAACEIVAWRRILYSENFPHVRLH
CCCCCHHCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEH
EDVRTLTAARLVSDLGGLPEIIVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECR
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PRWFAFENSSVLRTRGADRLLDELEALGYACWPCVVGAADIGANHVRKRSWLIGCDPEQL
CCEEEECCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHCCEEEECCHHHH
ADTRVAIPAGWIAAGGRIGGAEGDEGLGSFRSVPQPTLADADGLGRDEGRRRRRRGIDIS
CCCEEECCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
AADDPGDAAEVRRASRWPGRSAEPVAGPQQPPCGHDGHADGECGTAGQRVAQAQGPDDLG
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
SGPFRPQPDDRRSAAFRQAGAGNPADADEAGQPDGRLEPGLRPSEVADHGCGDGRGDDGS
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
GAGQMGRGAGAGGDLAEPWPDWNGGIAHHIRVDDGISAWLARTHLALGSRKGTSAASLIV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
EAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPANDAANDDGQSIDAGRGNAAPNDNAV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DEAASGSPTDHGGAP
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]