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The map label for this gene is cspA [C]

Identifier: 119385862

GI number: 119385862

Start: 321481

End: 321687

Strand: Direct

Name: cspA [C]

Synonym: Pden_3140

Alternate gene names: 119385862

Gene position: 321481-321687 (Clockwise)

Preceding gene: 119385861

Following gene: 119385863

Centisome position: 18.58

GC content: 55.56

Gene sequence:

>207_bases
ATGGCCAATGGCACCGTGAAATGGTTCAACTCCAATAAAGGCTTCGGCTTCATTGCCCCGGAAACGGGCTCGAAGGATGT
CTTCATCCATATTTCCGCGCTCGAGCGCGCCGGCATCCATCATCTGAACGATGGCCAGGCGATCGCCTATGATATCGAGC
GTGACCGCAATGGCCGGGAATCGGCAACGAATCTCGTTCTCTCCTGA

Upstream 100 bases:

>100_bases
TCTCTTGTTTTCGGACCCTGTTCCTTGCGGCTCAGCTTCGATCTTCAGCTTTGCCGGGCCGCTGCACTGTGTGAGCGGCA
TTTTTGAAAGGAACAGCACG

Downstream 100 bases:

>100_bases
GAGCAGCGCCGAAGGGTAATACAGGCGGGGTCGGCTTCATGCCGTCTCCGCTTTTCCTTGAGGAGGGTGGGCATGCGTTG
CCCGCCATTCCGACCGTTCC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MANGTVKWFNSNKGFGFIAPETGSKDVFIHISALERAGIHHLNDGQAIAYDIERDRNGRESATNLVLS

Sequences:

>Translated_68_residues
MANGTVKWFNSNKGFGFIAPETGSKDVFIHISALERAGIHHLNDGQAIAYDIERDRNGRESATNLVLS
>Mature_67_residues
ANGTVKWFNSNKGFGFIAPETGSKDVFIHISALERAGIHHLNDGQAIAYDIERDRNGRESATNLVLS

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=56, Percent_Identity=51.7857142857143, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=48.4375, Blast_Score=69, Evalue=4e-14,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=45.3125, Blast_Score=68, Evalue=9e-14,
Organism=Escherichia coli, GI1787839, Length=65, Percent_Identity=46.1538461538462, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1788126, Length=55, Percent_Identity=50.9090909090909, Blast_Score=65, Evalue=6e-13,
Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=42.8571428571429, Blast_Score=65, Evalue=8e-13,
Organism=Escherichia coli, GI1787834, Length=55, Percent_Identity=49.0909090909091, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7459; Mature: 7328

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGTVKWFNSNKGFGFIAPETGSKDVFIHISALERAGIHHLNDGQAIAYDIERDRNGRE
CCCCEEEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCEECCCCCEEEEEECCCCCCCC
SATNLVLS
CCCEEECC
>Mature Secondary Structure 
ANGTVKWFNSNKGFGFIAPETGSKDVFIHISALERAGIHHLNDGQAIAYDIERDRNGRE
CCCEEEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCEECCCCCEEEEEECCCCCCCC
SATNLVLS
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]