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The map label for this gene is dhbE [H]

Identifier: 119385732

GI number: 119385732

Start: 180896

End: 182563

Strand: Direct

Name: dhbE [H]

Synonym: Pden_3008

Alternate gene names: 119385732

Gene position: 180896-182563 (Clockwise)

Preceding gene: 119385727

Following gene: 119385733

Centisome position: 10.46

GC content: 69.48

Gene sequence:

>1668_bases
GTGGCAGATCACGCTCTGGACTGGCAGGCCCGCTGCCCGTTCTGGCCCCGGGACGTTGCTGACCGTTACCGCGAGGCCGG
GATCTGGCGATACGAGACATTCTTCGACCTTCTGGCGCGGCAAGCCGGGCGGCTGGGTCGGCGGATCGCCCTGATCGACG
GTGCGCGGCAGATCGGTTACGACCGGTTGCACTGCGATGCGCTGGCATTGGCGGGCGGGCTTTCGGCGCTGGGGCTGGTC
CGCGGCGACCGGGTGGTGGTGCAATTCCCCAATTGCGGGGAATTCGTCACGCTGTTCTTCGCGCTTTGCCGGCTGGGCGT
CGTGCCGGTGCTGGCCTTGCCCGGGCATCGCGACCTTGAGCTTGGCCAGTTTGCCGATTTCACCGGCGCCCGCGCGATCC
TGACGGCACAGGAGCCCAAGGGGTATGACATGGTCGCGCTGGCCCGGCGGGTGCAAGAGAGGGTTCCCTCGGTTCGGCAC
GTGGTGGCGCTGGGCGACGGCCGAGACGCGACGCCCTTCGACAGCCTGTTCCGTCCCGATCTCCCGTTGCCGGCCGGTCC
CAATGCCGAGGACGTGGCCTGTTTCCAGATCTCGGGCGGGACGACCGGCGTGCCCAAGCTGATCCCGCGCCGGCACATGG
AATATCTCTACAACATCCGGATGGCGGTGGCGGCCAGCGGGCTGGACGGCGATACGCGCTATCTGTGCGTTCTGCCGATG
ATGCACAACTTCCCGATGGCCTGTCCGGGTTTCCTGGGCACGCTGTGGGCCGGGGGCACGGTGGTCGTCGCACCCGAGCC
CGAGGCCGGGATCTGCTTCGACCTGATCGCCCGCCATCGCGTCACGATGACCGCGCTGGTGCCGCCGCTGGCGCTGCTGT
GGCTGGATGCGCAGGCCGGGCTGGGGGCGGACCTGTCGTCGCTGCAACTGCTGCAGGTCGGCGGCGCCAAGTTGAACCCG
TCCAGCGCCCGGCGCATCCGGCCGGAGCTGGGTTGCCAGCTGCAGCAGGTCTTCGGCATGGCCGAGGGGCTGGTCTGCTT
CACCGCGCTGACCGACAGCGAGGAGCGTATCGTGAACACCCAGGGCCGACCCATGTCGCCCTGGGACGAGCTGCGCATCA
TCCGCCCGGATGGGCGCGAGGCCGCCATCGGCGAGCCGGGCGAGCTGCAGGTCCGCGGCCCCTACACGATCCGCGGCTAT
TACGACATGGCCGGGCACAATGCCCAGGCCTTCACCGCCGACGGCTTCTATCGCTCGGGCGACATCGCGCGGCGCGACGC
CGACGGCGCGATTATCGTCGAGGGGCGGGCCAAGGACCAGGTGAACCGGGGCGGCGAAAAGATCGGCGTGGACGAGGTCG
AGGATCTGCTGGTCGCCCATGCCTCGGTGCTGGATGCGGCGGTGGTGGGGCGGCCCGATGCGCTGATGGGCGAAAGGCTT
TGTGCCTTCGTGCTGGCCGCGCCGGGCACCACGCCCCGGCCGGGTGCGCTGCGCCGCCATCTGGCCGAGGCCGGGCTTGC
CGCCTTCAAGATCCCCGACGAGGTGATCGTGCTGGATCGCTTTCCGCAAACCGGCGTCGGCAAGGTCAACAAGCGCCAGT
TGCGCGAGCAACTGCGCGAGGCGCATTTCCCCGATCCCGTCGCTGCCCGGCAGGAGGAGAGTGCATGA

Upstream 100 bases:

>100_bases
GCCAGTCGTCACGGTCGGGATTTCGGCGGAGCGGACATGGTTCTCCCTATCAAGGAAGATTTGCCCACCCTTCGCCAGCG
GATTGCGAAAGGAGATTGCT

Downstream 100 bases:

>100_bases
GCCCGGCCGTCGTGGCGCCCGAGGATCTGGCGCCGGACCTGGTCCCGCCGGAAGCCGTGGCGGGGCTGGGCGACGTCCGG
CGCGGCATCGACATGATCGA

Product: AMP-dependent synthetase and ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MADHALDWQARCPFWPRDVADRYREAGIWRYETFFDLLARQAGRLGRRIALIDGARQIGYDRLHCDALALAGGLSALGLV
RGDRVVVQFPNCGEFVTLFFALCRLGVVPVLALPGHRDLELGQFADFTGARAILTAQEPKGYDMVALARRVQERVPSVRH
VVALGDGRDATPFDSLFRPDLPLPAGPNAEDVACFQISGGTTGVPKLIPRRHMEYLYNIRMAVAASGLDGDTRYLCVLPM
MHNFPMACPGFLGTLWAGGTVVVAPEPEAGICFDLIARHRVTMTALVPPLALLWLDAQAGLGADLSSLQLLQVGGAKLNP
SSARRIRPELGCQLQQVFGMAEGLVCFTALTDSEERIVNTQGRPMSPWDELRIIRPDGREAAIGEPGELQVRGPYTIRGY
YDMAGHNAQAFTADGFYRSGDIARRDADGAIIVEGRAKDQVNRGGEKIGVDEVEDLLVAHASVLDAAVVGRPDALMGERL
CAFVLAAPGTTPRPGALRRHLAEAGLAAFKIPDEVIVLDRFPQTGVGKVNKRQLREQLREAHFPDPVAARQEESA

Sequences:

>Translated_555_residues
MADHALDWQARCPFWPRDVADRYREAGIWRYETFFDLLARQAGRLGRRIALIDGARQIGYDRLHCDALALAGGLSALGLV
RGDRVVVQFPNCGEFVTLFFALCRLGVVPVLALPGHRDLELGQFADFTGARAILTAQEPKGYDMVALARRVQERVPSVRH
VVALGDGRDATPFDSLFRPDLPLPAGPNAEDVACFQISGGTTGVPKLIPRRHMEYLYNIRMAVAASGLDGDTRYLCVLPM
MHNFPMACPGFLGTLWAGGTVVVAPEPEAGICFDLIARHRVTMTALVPPLALLWLDAQAGLGADLSSLQLLQVGGAKLNP
SSARRIRPELGCQLQQVFGMAEGLVCFTALTDSEERIVNTQGRPMSPWDELRIIRPDGREAAIGEPGELQVRGPYTIRGY
YDMAGHNAQAFTADGFYRSGDIARRDADGAIIVEGRAKDQVNRGGEKIGVDEVEDLLVAHASVLDAAVVGRPDALMGERL
CAFVLAAPGTTPRPGALRRHLAEAGLAAFKIPDEVIVLDRFPQTGVGKVNKRQLREQLREAHFPDPVAARQEESA
>Mature_554_residues
ADHALDWQARCPFWPRDVADRYREAGIWRYETFFDLLARQAGRLGRRIALIDGARQIGYDRLHCDALALAGGLSALGLVR
GDRVVVQFPNCGEFVTLFFALCRLGVVPVLALPGHRDLELGQFADFTGARAILTAQEPKGYDMVALARRVQERVPSVRHV
VALGDGRDATPFDSLFRPDLPLPAGPNAEDVACFQISGGTTGVPKLIPRRHMEYLYNIRMAVAASGLDGDTRYLCVLPMM
HNFPMACPGFLGTLWAGGTVVVAPEPEAGICFDLIARHRVTMTALVPPLALLWLDAQAGLGADLSSLQLLQVGGAKLNPS
SARRIRPELGCQLQQVFGMAEGLVCFTALTDSEERIVNTQGRPMSPWDELRIIRPDGREAAIGEPGELQVRGPYTIRGYY
DMAGHNAQAFTADGFYRSGDIARRDADGAIIVEGRAKDQVNRGGEKIGVDEVEDLLVAHASVLDAAVVGRPDALMGERLC
AFVLAAPGTTPRPGALRRHLAEAGLAAFKIPDEVIVLDRFPQTGVGKVNKRQLREQLREAHFPDPVAARQEESA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=24.7272727272727, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI38505220, Length=488, Percent_Identity=24.7950819672131, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=25.187969924812, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=25.187969924812, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI122937307, Length=483, Percent_Identity=25.879917184265, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI28416953, Length=526, Percent_Identity=25.8555133079848, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157311624, Length=481, Percent_Identity=23.0769230769231, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157311622, Length=481, Percent_Identity=23.0769230769231, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI58082049, Length=481, Percent_Identity=23.4927234927235, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI115511026, Length=490, Percent_Identity=22.6530612244898, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI8923896, Length=580, Percent_Identity=22.5862068965517, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI42544132, Length=372, Percent_Identity=23.6559139784946, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI253970489, Length=131, Percent_Identity=35.1145038167939, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786810, Length=531, Percent_Identity=46.3276836158192, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=26.8939393939394, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788107, Length=567, Percent_Identity=25.5731922398589, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI221142682, Length=510, Percent_Identity=25.2941176470588, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790505, Length=585, Percent_Identity=25.982905982906, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=24.4565217391304, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786529, Length=525, Percent_Identity=23.047619047619, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786801, Length=497, Percent_Identity=23.3400402414487, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=29.2910447761194, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=553, Percent_Identity=28.75226039783, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=553, Percent_Identity=28.75226039783, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=553, Percent_Identity=28.75226039783, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=494, Percent_Identity=26.7206477732794, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=557, Percent_Identity=25.3141831238779, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=539, Percent_Identity=24.860853432282, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=374, Percent_Identity=25.1336898395722, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=22.8, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=497, Percent_Identity=23.1388329979879, Blast_Score=112, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=518, Percent_Identity=23.1660231660232, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=25.5747126436782, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=569, Percent_Identity=23.9015817223199, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=569, Percent_Identity=23.9015817223199, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=24.5945945945946, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=526, Percent_Identity=25.2851711026616, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=536, Percent_Identity=24.2537313432836, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=27.8388278388278, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21355181, Length=514, Percent_Identity=25.2918287937743, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI18859661, Length=502, Percent_Identity=24.5019920318725, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21355225, Length=565, Percent_Identity=23.3628318584071, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667955, Length=571, Percent_Identity=23.1173380035026, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI62472339, Length=545, Percent_Identity=22.9357798165138, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24656500, Length=402, Percent_Identity=25.8706467661692, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=488, Percent_Identity=21.7213114754098, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648260, Length=490, Percent_Identity=21.6326530612245, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=394, Percent_Identity=23.0964467005076, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=497, Percent_Identity=21.7303822937626, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24667959, Length=281, Percent_Identity=25.9786476868327, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24653035, Length=381, Percent_Identity=22.8346456692913, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648253, Length=376, Percent_Identity=22.3404255319149, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648255, Length=388, Percent_Identity=22.4226804123711, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 60363; Mature: 60232

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHALDWQARCPFWPRDVADRYREAGIWRYETFFDLLARQAGRLGRRIALIDGARQIGY
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHCC
DRLHCDALALAGGLSALGLVRGDRVVVQFPNCGEFVTLFFALCRLGVVPVLALPGHRDLE
CHHHHHHHHHHCCHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCC
LGQFADFTGARAILTAQEPKGYDMVALARRVQERVPSVRHVVALGDGRDATPFDSLFRPD
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCC
LPLPAGPNAEDVACFQISGGTTGVPKLIPRRHMEYLYNIRMAVAASGLDGDTRYLCVLPM
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEH
MHNFPMACPGFLGTLWAGGTVVVAPEPEAGICFDLIARHRVTMTALVPPLALLWLDAQAG
HHCCCCCCCHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LGADLSSLQLLQVGGAKLNPSSARRIRPELGCQLQQVFGMAEGLVCFTALTDSEERIVNT
CCCCHHHHHEEECCCCCCCCCHHHHCCHHHCHHHHHHHCHHCCEEEEEEECCCHHHHCCC
QGRPMSPWDELRIIRPDGREAAIGEPGELQVRGPYTIRGYYDMAGHNAQAFTADGFYRSG
CCCCCCCHHHEEEECCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCEEECCCCCCCC
DIARRDADGAIIVEGRAKDQVNRGGEKIGVDEVEDLLVAHASVLDAAVVGRPDALMGERL
CCCEECCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CAFVLAAPGTTPRPGALRRHLAEAGLAAFKIPDEVIVLDRFPQTGVGKVNKRQLREQLRE
HHHHEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHH
AHFPDPVAARQEESA
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADHALDWQARCPFWPRDVADRYREAGIWRYETFFDLLARQAGRLGRRIALIDGARQIGY
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHCC
DRLHCDALALAGGLSALGLVRGDRVVVQFPNCGEFVTLFFALCRLGVVPVLALPGHRDLE
CHHHHHHHHHHCCHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCC
LGQFADFTGARAILTAQEPKGYDMVALARRVQERVPSVRHVVALGDGRDATPFDSLFRPD
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHCCCC
LPLPAGPNAEDVACFQISGGTTGVPKLIPRRHMEYLYNIRMAVAASGLDGDTRYLCVLPM
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEH
MHNFPMACPGFLGTLWAGGTVVVAPEPEAGICFDLIARHRVTMTALVPPLALLWLDAQAG
HHCCCCCCCHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LGADLSSLQLLQVGGAKLNPSSARRIRPELGCQLQQVFGMAEGLVCFTALTDSEERIVNT
CCCCHHHHHEEECCCCCCCCCHHHHCCHHHCHHHHHHHCHHCCEEEEEEECCCHHHHCCC
QGRPMSPWDELRIIRPDGREAAIGEPGELQVRGPYTIRGYYDMAGHNAQAFTADGFYRSG
CCCCCCCHHHEEEECCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCEEECCCCCCCC
DIARRDADGAIIVEGRAKDQVNRGGEKIGVDEVEDLLVAHASVLDAAVVGRPDALMGERL
CCCEECCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CAFVLAAPGTTPRPGALRRHLAEAGLAAFKIPDEVIVLDRFPQTGVGKVNKRQLREQLRE
HHHHEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHH
AHFPDPVAARQEESA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]