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The map label for this gene is thiC

Identifier: 119385669

GI number: 119385669

Start: 111383

End: 113182

Strand: Direct

Name: thiC

Synonym: Pden_2944

Alternate gene names: 119385669

Gene position: 111383-113182 (Clockwise)

Preceding gene: 119385668

Following gene: 119385684

Centisome position: 6.44

GC content: 68.22

Gene sequence:

>1800_bases
ATGAGCCAGACCATCCCGCAAATCACCACCGGCCCGCTGCCCGGATCGCGCAAGATCCATGTGCCGGGCAGCCTGCACGA
CATCCGCGTGCCCATGCGCGAGATCGCCGTCTCGAACGAGCCGCCGCTGGTCGTCTATGACAGCTCCGGCCCCTATACCG
ACGCGGCGGTGCAGGCCGACATCGCCCGCGGCCTGCCGGACCTGCGCGGCGACTGGCAGCTGCGGCGCGGCGACGTGGCG
CCCTATCCCGGCCGGCAGGTCACCGCCGCCGACAACGGCTTTGCCGAGGGCGCGCGGCTGACCCCCGCCTTTCCGCTGCG
CCGCGATCCCCTGCGCGCGGCGGCGGGCCGGGCGGTGACGCAGCTGGCCTATGCCCGCGCCGGCATCGTCACCCCCGAGA
TGGAGTTCGCGGCCATCCGCGAGAACGAGGGGCGGCTGGTCGCCCATGCCCGCGACGGCGCGCCGATGGGGGCCGAGCTG
CCCGATCTGGTGACGCCGGAATTCGTGCGGGCCGAGATCGCCGCCGGCCGCGCCATCATCCCGGCCAACATCAACCACCG
CGAGTTGGAGCCGATGATCATCGGCCGCAATTTCAAGGTCAAGATCAACGCCAATATCGGCAACTCCGCCGTCACCTCCA
GCATGGAGGAGGAGGTCGAGAAGATGGTCTGGGCGATCCGCTGGGGCGCGGACACGGTGATGGACCTGTCCACCGGCCGC
AACATCCACAACATCCGCGACTGGATCATCCGCAACGCGCCGGTGCCCATCGGCACCGTGCCGCTGTATCAGGCGCTGGA
GAAGGTCGGCGGCGTGGCCGAGGATCTGAGCTGGGAGGTGTTTCGGGACACGCTGGTCGAACAGGCCGAGCAGGGCGTGG
ACTATTTCACCATCCATGCCGGGGTGCGGCTGCACATGATCCCGCTGACCGCGCGGCGGGTGACGGGGATCGTCAGCCGC
GGCGGCTCGATCATGGCGAAATGGTGCCTGCACCACCACCGCGAGAGCTTCCTGTATGAGCGCTTCGACGAGATCTGCGA
GATCATGCAGGCCTATGACGTCAGCTTCAGCCTGGGCGACGGGCTGCGTCCCGGCTCGATCGCCGATGCCAATGACGAGG
CGCAATGCGCCGAACTGCGCACCCTGGGCGAGCTGACGAAGATCGCCTGGGCCCGGGATTGCCAGGTGATGATCGAGGGG
CCGGGCCATGTGCCGATGCACAAGATCAAGGCCAATATGGAGGAGCAGCTGCGGCATTGCCACGAGGCGCCGTTCTATAC
GCTTGGCCCGCTGACCACCGATATCGCACCGGGCTACGACCACATCACCTCGGCCATCGGGGCGGCGATGATCGGCTGGT
TCGGCACGGCGATGCTGTGCTATGTCACGCCCAAGGAGCATCTGGGCCTGCCCGACCGCGACGACGTCAAGACCGGCGTC
ATCACCTACAAGCTGGCCGCCCATGCCGCCGATCTGGCCAAGGGCCATCCCGGGGCGCAGCGGCGCGACGATGCGCTGTC
GCGCGCGCGGTTCGAGTTCCGCTGGCAGGACCAGTTCAACCTGGGCCTGGACCCGGACACCGCGCAGGCCATGCATGACG
AGACCCTGCCGAAAGAGGCGCACAAGCTGGCGCATTTCTGTTCGATGTGCGGGCCGAAGTTCTGCTCGATGCGGATCTCG
CACGACATCCGGGCCGAGGCTGAAAAGCAGGCCGGCATGGCGCGCATGGCCGAGAAGTTCCGCGAGGGCGGGGCGCTTTA
CCTGCCGGTTGCGGAAAGCGTGGCGGAGGCGGCCGATTGA

Upstream 100 bases:

>100_bases
GTCCTTTCGGGCCATCGGGCCCTTTGCGCGTGATCCGCGGGGCCGGCATGGTCCCTTTCTGCCCTGTTTTCCTCTGGGTC
TTTCGCCGAAGGAGGACCAG

Downstream 100 bases:

>100_bases
TCCGCGGGGGCGCGGCTTCTGGCCGCGCCCGCCCGCCTTGGCGTTTCATACGCGGTCAGGCAAGAAGCAGGGCGAGGCCG
CGGAAGAACGCCCAGATCAC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSQTIPQITTGPLPGSRKIHVPGSLHDIRVPMREIAVSNEPPLVVYDSSGPYTDAAVQADIARGLPDLRGDWQLRRGDVA
PYPGRQVTAADNGFAEGARLTPAFPLRRDPLRAAAGRAVTQLAYARAGIVTPEMEFAAIRENEGRLVAHARDGAPMGAEL
PDLVTPEFVRAEIAAGRAIIPANINHRELEPMIIGRNFKVKINANIGNSAVTSSMEEEVEKMVWAIRWGADTVMDLSTGR
NIHNIRDWIIRNAPVPIGTVPLYQALEKVGGVAEDLSWEVFRDTLVEQAEQGVDYFTIHAGVRLHMIPLTARRVTGIVSR
GGSIMAKWCLHHHRESFLYERFDEICEIMQAYDVSFSLGDGLRPGSIADANDEAQCAELRTLGELTKIAWARDCQVMIEG
PGHVPMHKIKANMEEQLRHCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGV
ITYKLAAHAADLAKGHPGAQRRDDALSRARFEFRWQDQFNLGLDPDTAQAMHDETLPKEAHKLAHFCSMCGPKFCSMRIS
HDIRAEAEKQAGMARMAEKFREGGALYLPVAESVAEAAD

Sequences:

>Translated_599_residues
MSQTIPQITTGPLPGSRKIHVPGSLHDIRVPMREIAVSNEPPLVVYDSSGPYTDAAVQADIARGLPDLRGDWQLRRGDVA
PYPGRQVTAADNGFAEGARLTPAFPLRRDPLRAAAGRAVTQLAYARAGIVTPEMEFAAIRENEGRLVAHARDGAPMGAEL
PDLVTPEFVRAEIAAGRAIIPANINHRELEPMIIGRNFKVKINANIGNSAVTSSMEEEVEKMVWAIRWGADTVMDLSTGR
NIHNIRDWIIRNAPVPIGTVPLYQALEKVGGVAEDLSWEVFRDTLVEQAEQGVDYFTIHAGVRLHMIPLTARRVTGIVSR
GGSIMAKWCLHHHRESFLYERFDEICEIMQAYDVSFSLGDGLRPGSIADANDEAQCAELRTLGELTKIAWARDCQVMIEG
PGHVPMHKIKANMEEQLRHCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGV
ITYKLAAHAADLAKGHPGAQRRDDALSRARFEFRWQDQFNLGLDPDTAQAMHDETLPKEAHKLAHFCSMCGPKFCSMRIS
HDIRAEAEKQAGMARMAEKFREGGALYLPVAESVAEAAD
>Mature_598_residues
SQTIPQITTGPLPGSRKIHVPGSLHDIRVPMREIAVSNEPPLVVYDSSGPYTDAAVQADIARGLPDLRGDWQLRRGDVAP
YPGRQVTAADNGFAEGARLTPAFPLRRDPLRAAAGRAVTQLAYARAGIVTPEMEFAAIRENEGRLVAHARDGAPMGAELP
DLVTPEFVRAEIAAGRAIIPANINHRELEPMIIGRNFKVKINANIGNSAVTSSMEEEVEKMVWAIRWGADTVMDLSTGRN
IHNIRDWIIRNAPVPIGTVPLYQALEKVGGVAEDLSWEVFRDTLVEQAEQGVDYFTIHAGVRLHMIPLTARRVTGIVSRG
GSIMAKWCLHHHRESFLYERFDEICEIMQAYDVSFSLGDGLRPGSIADANDEAQCAELRTLGELTKIAWARDCQVMIEGP
GHVPMHKIKANMEEQLRHCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGVI
TYKLAAHAADLAKGHPGAQRRDDALSRARFEFRWQDQFNLGLDPDTAQAMHDETLPKEAHKLAHFCSMCGPKFCSMRISH
DIRAEAEKQAGMARMAEKFREGGALYLPVAESVAEAAD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=605, Percent_Identity=66.6115702479339, Blast_Score=814, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_PARDP (A1B684)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_916724.1
- STRING:   A1B684
- GeneID:   4581511
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_2944
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   A1B684
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 65873; Mature: 65742

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: BINDING 205-205 BINDING 234-234 BINDING 263-263 BINDING 299-299 BINDING 399-399 BINDING 426-426

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTIPQITTGPLPGSRKIHVPGSLHDIRVPMREIAVSNEPPLVVYDSSGPYTDAAVQAD
CCCCCCCCCCCCCCCCCEEECCCCCHHHHCCHHHHHCCCCCCEEEECCCCCCCHHHHHHH
IARGLPDLRGDWQLRRGDVAPYPGRQVTAADNGFAEGARLTPAFPLRRDPLRAAAGRAVT
HHHCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QLAYARAGIVTPEMEFAAIRENEGRLVAHARDGAPMGAELPDLVTPEFVRAEIAAGRAII
HHHHHHHCCCCCCCHHEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
PANINHRELEPMIIGRNFKVKINANIGNSAVTSSMEEEVEKMVWAIRWGADTVMDLSTGR
ECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
NIHNIRDWIIRNAPVPIGTVPLYQALEKVGGVAEDLSWEVFRDTLVEQAEQGVDYFTIHA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GVRLHMIPLTARRVTGIVSRGGSIMAKWCLHHHRESFLYERFDEICEIMQAYDVSFSLGD
CCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
GLRPGSIADANDEAQCAELRTLGELTKIAWARDCQVMIEGPGHVPMHKIKANMEEQLRHC
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHH
HEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGV
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCE
ITYKLAAHAADLAKGHPGAQRRDDALSRARFEFRWQDQFNLGLDPDTAQAMHDETLPKEA
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHCCHHHH
HKLAHFCSMCGPKFCSMRISHDIRAEAEKQAGMARMAEKFREGGALYLPVAESVAEAAD
HHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCC
>Mature Secondary Structure 
SQTIPQITTGPLPGSRKIHVPGSLHDIRVPMREIAVSNEPPLVVYDSSGPYTDAAVQAD
CCCCCCCCCCCCCCCCEEECCCCCHHHHCCHHHHHCCCCCCEEEECCCCCCCHHHHHHH
IARGLPDLRGDWQLRRGDVAPYPGRQVTAADNGFAEGARLTPAFPLRRDPLRAAAGRAVT
HHHCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QLAYARAGIVTPEMEFAAIRENEGRLVAHARDGAPMGAELPDLVTPEFVRAEIAAGRAII
HHHHHHHCCCCCCCHHEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
PANINHRELEPMIIGRNFKVKINANIGNSAVTSSMEEEVEKMVWAIRWGADTVMDLSTGR
ECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
NIHNIRDWIIRNAPVPIGTVPLYQALEKVGGVAEDLSWEVFRDTLVEQAEQGVDYFTIHA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GVRLHMIPLTARRVTGIVSRGGSIMAKWCLHHHRESFLYERFDEICEIMQAYDVSFSLGD
CCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
GLRPGSIADANDEAQCAELRTLGELTKIAWARDCQVMIEGPGHVPMHKIKANMEEQLRHC
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHH
HEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGV
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCE
ITYKLAAHAADLAKGHPGAQRRDDALSRARFEFRWQDQFNLGLDPDTAQAMHDETLPKEA
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHCCHHHH
HKLAHFCSMCGPKFCSMRISHDIRAEAEKQAGMARMAEKFREGGALYLPVAESVAEAAD
HHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA