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The map label for this gene is dapE

Identifier: 119385567

GI number: 119385567

Start: 10132

End: 11280

Strand: Direct

Name: dapE

Synonym: Pden_2842

Alternate gene names: 119385567

Gene position: 10132-11280 (Clockwise)

Preceding gene: 119385566

Following gene: 119385568

Centisome position: 0.59

GC content: 69.36

Gene sequence:

>1149_bases
ATGACCCCGATCCCCGATCCCGCCCAACTGACCGCCAGGCTGATCCGCTGCGCCTCGGTCACGCCCGACGAGGGCGGCGC
GCTGGTCTTGCTGGCCGATGTGCTGGGCGCGGCGGGGTTCGAATGCCACCGCGTCGACCGCGAGGGCGTGCCGAACCTGT
TCGCGCGCTGGGGGGCACAGGGGGCCAGGACCTTCGGCTTCAACGGCCATACCGACGTGGTGCCGCCGGGAGATCCGGCC
AGCTGGACGCATCCGCCCTTTTCCGGGCACGAGGCCGAGGGCTGGATCTGGGGCCGCGGCGCCACCGACATGAAATCCGG
CGTCGCCGCCTTTGCCGCCGCCGCCATCGGCTTCGTGACCCAGACCCCGCCGCCGGACGGCGCGGTGATCCTGGCCATCA
CCGGCGACGAGGAGGGGCCCGGCAAGCACGGCACCCGCGCGCTGCTCGACTGGATGGCGGCGCGGGGCGAGCGGATGGAT
GTCTGCATCGTTGGCGAGCCTTCCAACCCCGACCGCATGGGCGAGATGATCAAGATCGGCCGCCGCGGATCGATGACCTT
GCAGATCGAGGCGCACGGCATCCAGGGCCACGCCGCCTATCCGCATCGGGCCCGCAACCCGATCCACGCGCTGCTGCGGC
TGCTGCACGAGCTGACCGACGCGCCGCTGGACGAGGGGACCGAGCATTTCGATCCCTCGGGATTGCAGGTGACGACGGTC
GATTGCGGCAACCCGGCCTCGAACGTGATCCCGGAACGGGCGCGTGCGGTGATCAACATCCGTTTCAACGACGCGCATAC
GGCCGAAAGCCTCGACCGCATGATCCGTGCCCGCGCGGCGGCGATCTCGGCCGAGACCAAGGTGGACTTCGCCATCTCGA
CCGATGTGTCGGGCGAAAGCTTCCTGACCGCGCCCGGCCCCTTCGTCGATCTGGTCGCCGGCGTGGTGCGCGAGGAGACG
GGGCTGGACCCTGTGCTCTCGACCTCGGGCGGCACCTCGGATGCGCGTTTCGTCAAGGATCATTGCCCGGTGCTTGAATT
CGGGCTGGTCGGGCATTTCATGCATCAGGTCGACGAACGCGTGCCGGCCGACCAGGTCCGCCAGCTTGCCCGCATCTATC
GCCGCATCCTGGAGCGTTACTTCGCATGA

Upstream 100 bases:

>100_bases
CTGGTCCTGCTGGTGCTGACCGGCGCCAACCTGCTGCGCCGCGCGCTGGAGCTTGCGGGTCCGGCGGTGTAACCAGGGGA
CCAAAAGCGAAAGGCCCGCC

Downstream 100 bases:

>100_bases
TCGAGAAAACCACCGACCTCGCGACCTGCCGCGCCATCCGGCACGAGGTCTTCGTCATCGAGCAAGCCGTCCCCGAGGCC
GAGGAATGGGACGGCCGCGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTPIPDPAQLTARLIRCASVTPDEGGALVLLADVLGAAGFECHRVDREGVPNLFARWGAQGARTFGFNGHTDVVPPGDPA
SWTHPPFSGHEAEGWIWGRGATDMKSGVAAFAAAAIGFVTQTPPPDGAVILAITGDEEGPGKHGTRALLDWMAARGERMD
VCIVGEPSNPDRMGEMIKIGRRGSMTLQIEAHGIQGHAAYPHRARNPIHALLRLLHELTDAPLDEGTEHFDPSGLQVTTV
DCGNPASNVIPERARAVINIRFNDAHTAESLDRMIRARAAAISAETKVDFAISTDVSGESFLTAPGPFVDLVAGVVREET
GLDPVLSTSGGTSDARFVKDHCPVLEFGLVGHFMHQVDERVPADQVRQLARIYRRILERYFA

Sequences:

>Translated_382_residues
MTPIPDPAQLTARLIRCASVTPDEGGALVLLADVLGAAGFECHRVDREGVPNLFARWGAQGARTFGFNGHTDVVPPGDPA
SWTHPPFSGHEAEGWIWGRGATDMKSGVAAFAAAAIGFVTQTPPPDGAVILAITGDEEGPGKHGTRALLDWMAARGERMD
VCIVGEPSNPDRMGEMIKIGRRGSMTLQIEAHGIQGHAAYPHRARNPIHALLRLLHELTDAPLDEGTEHFDPSGLQVTTV
DCGNPASNVIPERARAVINIRFNDAHTAESLDRMIRARAAAISAETKVDFAISTDVSGESFLTAPGPFVDLVAGVVREET
GLDPVLSTSGGTSDARFVKDHCPVLEFGLVGHFMHQVDERVPADQVRQLARIYRRILERYFA
>Mature_381_residues
TPIPDPAQLTARLIRCASVTPDEGGALVLLADVLGAAGFECHRVDREGVPNLFARWGAQGARTFGFNGHTDVVPPGDPAS
WTHPPFSGHEAEGWIWGRGATDMKSGVAAFAAAAIGFVTQTPPPDGAVILAITGDEEGPGKHGTRALLDWMAARGERMDV
CIVGEPSNPDRMGEMIKIGRRGSMTLQIEAHGIQGHAAYPHRARNPIHALLRLLHELTDAPLDEGTEHFDPSGLQVTTVD
CGNPASNVIPERARAVINIRFNDAHTAESLDRMIRARAAAISAETKVDFAISTDVSGESFLTAPGPFVDLVAGVVREETG
LDPVLSTSGGTSDARFVKDHCPVLEFGLVGHFMHQVDERVPADQVRQLARIYRRILERYFA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=40.8, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1789236, Length=287, Percent_Identity=24.0418118466899, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790395, Length=188, Percent_Identity=33.5106382978723, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24649206, Length=345, Percent_Identity=24.3478260869565, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24649212, Length=341, Percent_Identity=24.0469208211144, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24649210, Length=341, Percent_Identity=24.633431085044, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_PARDP (A1B5Y2)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_916622.1
- ProteinModelPortal:   A1B5Y2
- SMR:   A1B5Y2
- STRING:   A1B5Y2
- MEROPS:   M20.010
- GeneID:   4581429
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_2842
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   RIHAINE
- PhylomeDB:   A1B5Y2
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41002; Mature: 40871

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 72-72 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIPDPAQLTARLIRCASVTPDEGGALVLLADVLGAAGFECHRVDREGVPNLFARWGAQ
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCHHHHHHHCCC
GARTFGFNGHTDVVPPGDPASWTHPPFSGHEAEGWIWGRGATDMKSGVAAFAAAAIGFVT
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHC
QTPPPDGAVILAITGDEEGPGKHGTRALLDWMAARGERMDVCIVGEPSNPDRMGEMIKIG
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHC
RRGSMTLQIEAHGIQGHAAYPHRARNPIHALLRLLHELTDAPLDEGTEHFDPSGLQVTTV
CCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DCGNPASNVIPERARAVINIRFNDAHTAESLDRMIRARAAAISAETKVDFAISTDVSGES
ECCCCHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FLTAPGPFVDLVAGVVREETGLDPVLSTSGGTSDARFVKDHCPVLEFGLVGHFMHQVDER
EEECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHC
VPADQVRQLARIYRRILERYFA
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPIPDPAQLTARLIRCASVTPDEGGALVLLADVLGAAGFECHRVDREGVPNLFARWGAQ
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCHHHHHHHCCC
GARTFGFNGHTDVVPPGDPASWTHPPFSGHEAEGWIWGRGATDMKSGVAAFAAAAIGFVT
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHC
QTPPPDGAVILAITGDEEGPGKHGTRALLDWMAARGERMDVCIVGEPSNPDRMGEMIKIG
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHC
RRGSMTLQIEAHGIQGHAAYPHRARNPIHALLRLLHELTDAPLDEGTEHFDPSGLQVTTV
CCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DCGNPASNVIPERARAVINIRFNDAHTAESLDRMIRARAAAISAETKVDFAISTDVSGES
ECCCCHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FLTAPGPFVDLVAGVVREETGLDPVLSTSGGTSDARFVKDHCPVLEFGLVGHFMHQVDER
EEECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHC
VPADQVRQLARIYRRILERYFA
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA