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The map label for this gene is murD

Identifier: 119358487

GI number: 119358487

Start: 3113310

End: 3114704

Strand: Reverse

Name: murD

Synonym: Cpha266_2723

Alternate gene names: 119358487

Gene position: 3114704-3113310 (Counterclockwise)

Preceding gene: 119358488

Following gene: 119358486

Centisome position: 99.39

GC content: 54.41

Gene sequence:

>1395_bases
ATGGACGTTGCGGGTAAAAAAGTAGCCGTGCTTGGCGCAAGACGAAGCGGTATGGCAGTTGCCGAACTGCTTTCGCTTAA
AGGAGCCTCGGTCTTTGTAAGCGAGCTCGGTACAATTGGAGCGCACGAATCAGCCCGTCTGCAGGCATTGCGGATCCCTT
TTGAAGAGGGAGGGCATTCCGACAACGTGCTTTGCACGGATTTCTGTGTGATCAGTCCGGGGATTCCCGGGCGGGCCCCG
GTTGTCAGAGCGATGCTCGAAAAAGGCATTCCCCTATTCAGCGAGATCGAGGTTGCGTACTGGTTCTGCAAGGCGAGAAT
TATAGGTATTACCGGCACGGACGGCAAAACAACGACAGCGACGCTGGTGCACCGTATTTTTGAAACGGACGGGATGACGC
ATCGATACCGGGCGTTCAGCGTAGGCAATATCGGTCAGCCATTCTCATCGAGAGTGCTCACCATGCGCCCCGAAGACGTT
GCCGTTATTGAGCTGAGCAGTTATCAGCTCGAAGGATGCTGCACCTTCAGGCCCGACGTATCGGTGATAACCAATATAAC
CCCGGATCACCTCGACCGGTATGAAGGGGAGCTGCATAACTACGCGCAAACAAAGTATCGAATTTACGCCCATCAGGGCA
AAGAGGACACGCTGGTCTATAATGATGACGACCCGCTTCTTCACGACCATTTTGCCTCAAACAGGGAGACCCTCCCGTGC
CGCATCGTTCCGTTCGGTATCGGATGCAAGCCGGAACATGCGGGTTTTGCTGCGGCGGTAAGGTTTTGCGACCACAGGAT
CGTTTTTGACGCCGGGGCAAAGAGCGAGAACATTATCGAAGCGGAAGATTTTCTGAAACGGAGTTTTCGTGGCCGTCACA
ATATTGCCAATGCCCTTGCCGCCGTCGCTGCCGCTCGTGCGCTCGGTATCGGCAACGAAGCCATCCGTTCTGCGCTTCAG
GGATTCAGCGGAGTCGAACATCGTCAGGAATTTGTGCGGACGCTTGACGGGAGCGACTGGATCAATGACTCAAAAGCAAC
CAACATCAATGCGCTTTCTCAGGCACTCGAAACCGTACCCGGACGGATGGTGCTTATTGCCGGAGGAAGAGACAAAGGCA
GCGATTACAGGGAGATCGCCGGGATTGTCAGAAAAAAAGTCGCGGCTCTTGTTGCAATAGGCGAAGCTCGCGAAAAACTC
TGCGCCGCATACGGAGGCATGGTCGATGTCAGATCTGCCAACTCCCTTGAAGAGGCGGTTTCTCTTGCCAGAGCCTTGGT
TGAACCCGGCCAGACGGTGCTGTTTTCGCCGGGGTGTTCTAGTTTCGACATGTTTGAGGATTTCGAGGATCGGGGGAGGC
AGTTTAAAAAATTAACGACGGATCTGCGCTCATGA

Upstream 100 bases:

>100_bases
TTCAGCTGAAAGGATGGGCAGAACAGAAAATCGTTATCAGGTTCTGGATTATAAGCATTCTGTTTTTTTTAACGAGTCTT
ATGACCTTAAAGCTCAGGTG

Downstream 100 bases:

>100_bases
TACATAACGCCAAAGAGATCGGTAATGCCGAACCCCCTCCGGCACTATCCGCTCAATCACAGGGGGAGGTTATCGCAGGC
AAACTGCTGTTGCTTGTCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDVAGKKVAVLGARRSGMAVAELLSLKGASVFVSELGTIGAHESARLQALRIPFEEGGHSDNVLCTDFCVISPGIPGRAP
VVRAMLEKGIPLFSEIEVAYWFCKARIIGITGTDGKTTTATLVHRIFETDGMTHRYRAFSVGNIGQPFSSRVLTMRPEDV
AVIELSSYQLEGCCTFRPDVSVITNITPDHLDRYEGELHNYAQTKYRIYAHQGKEDTLVYNDDDPLLHDHFASNRETLPC
RIVPFGIGCKPEHAGFAAAVRFCDHRIVFDAGAKSENIIEAEDFLKRSFRGRHNIANALAAVAAARALGIGNEAIRSALQ
GFSGVEHRQEFVRTLDGSDWINDSKATNINALSQALETVPGRMVLIAGGRDKGSDYREIAGIVRKKVAALVAIGEAREKL
CAAYGGMVDVRSANSLEEAVSLARALVEPGQTVLFSPGCSSFDMFEDFEDRGRQFKKLTTDLRS

Sequences:

>Translated_464_residues
MDVAGKKVAVLGARRSGMAVAELLSLKGASVFVSELGTIGAHESARLQALRIPFEEGGHSDNVLCTDFCVISPGIPGRAP
VVRAMLEKGIPLFSEIEVAYWFCKARIIGITGTDGKTTTATLVHRIFETDGMTHRYRAFSVGNIGQPFSSRVLTMRPEDV
AVIELSSYQLEGCCTFRPDVSVITNITPDHLDRYEGELHNYAQTKYRIYAHQGKEDTLVYNDDDPLLHDHFASNRETLPC
RIVPFGIGCKPEHAGFAAAVRFCDHRIVFDAGAKSENIIEAEDFLKRSFRGRHNIANALAAVAAARALGIGNEAIRSALQ
GFSGVEHRQEFVRTLDGSDWINDSKATNINALSQALETVPGRMVLIAGGRDKGSDYREIAGIVRKKVAALVAIGEAREKL
CAAYGGMVDVRSANSLEEAVSLARALVEPGQTVLFSPGCSSFDMFEDFEDRGRQFKKLTTDLRS
>Mature_464_residues
MDVAGKKVAVLGARRSGMAVAELLSLKGASVFVSELGTIGAHESARLQALRIPFEEGGHSDNVLCTDFCVISPGIPGRAP
VVRAMLEKGIPLFSEIEVAYWFCKARIIGITGTDGKTTTATLVHRIFETDGMTHRYRAFSVGNIGQPFSSRVLTMRPEDV
AVIELSSYQLEGCCTFRPDVSVITNITPDHLDRYEGELHNYAQTKYRIYAHQGKEDTLVYNDDDPLLHDHFASNRETLPC
RIVPFGIGCKPEHAGFAAAVRFCDHRIVFDAGAKSENIIEAEDFLKRSFRGRHNIANALAAVAAARALGIGNEAIRSALQ
GFSGVEHRQEFVRTLDGSDWINDSKATNINALSQALETVPGRMVLIAGGRDKGSDYREIAGIVRKKVAALVAIGEAREKL
CAAYGGMVDVRSANSLEEAVSLARALVEPGQTVLFSPGCSSFDMFEDFEDRGRQFKKLTTDLRS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=469, Percent_Identity=30.4904051172708, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CHLPD (A1BJY0)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_913131.1
- ProteinModelPortal:   A1BJY0
- SMR:   A1BJY0
- STRING:   A1BJY0
- GeneID:   4570697
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2723
- NMPDR:   fig|290317.7.peg.2844
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50600; Mature: 50600

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAGKKVAVLGARRSGMAVAELLSLKGASVFVSELGTIGAHESARLQALRIPFEEGGHS
CCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCC
DNVLCTDFCVISPGIPGRAPVVRAMLEKGIPLFSEIEVAYWFCKARIIGITGTDGKTTTA
CCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEEEEECCCCCHHHH
TLVHRIFETDGMTHRYRAFSVGNIGQPFSSRVLTMRPEDVAVIELSSYQLEGCCTFRPDV
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCEEECEEEEECCCC
SVITNITPDHLDRYEGELHNYAQTKYRIYAHQGKEDTLVYNDDDPLLHDHFASNRETLPC
CEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCE
RIVPFGIGCKPEHAGFAAAVRFCDHRIVFDAGAKSENIIEAEDFLKRSFRGRHNIANALA
EEEEECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
AVAAARALGIGNEAIRSALQGFSGVEHRQEFVRTLDGSDWINDSKATNINALSQALETVP
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
GRMVLIAGGRDKGSDYREIAGIVRKKVAALVAIGEAREKLCAAYGGMVDVRSANSLEEAV
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
SLARALVEPGQTVLFSPGCSSFDMFEDFEDRGRQFKKLTTDLRS
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVAGKKVAVLGARRSGMAVAELLSLKGASVFVSELGTIGAHESARLQALRIPFEEGGHS
CCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCC
DNVLCTDFCVISPGIPGRAPVVRAMLEKGIPLFSEIEVAYWFCKARIIGITGTDGKTTTA
CCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEEEEECCCCCHHHH
TLVHRIFETDGMTHRYRAFSVGNIGQPFSSRVLTMRPEDVAVIELSSYQLEGCCTFRPDV
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCEEECEEEEECCCC
SVITNITPDHLDRYEGELHNYAQTKYRIYAHQGKEDTLVYNDDDPLLHDHFASNRETLPC
CEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCHHHHHCCCCCCCCE
RIVPFGIGCKPEHAGFAAAVRFCDHRIVFDAGAKSENIIEAEDFLKRSFRGRHNIANALA
EEEEECCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
AVAAARALGIGNEAIRSALQGFSGVEHRQEFVRTLDGSDWINDSKATNINALSQALETVP
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
GRMVLIAGGRDKGSDYREIAGIVRKKVAALVAIGEAREKLCAAYGGMVDVRSANSLEEAV
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
SLARALVEPGQTVLFSPGCSSFDMFEDFEDRGRQFKKLTTDLRS
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA