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The map label for this gene is capD [H]

Identifier: 119358399

GI number: 119358399

Start: 3023817

End: 3025865

Strand: Reverse

Name: capD [H]

Synonym: Cpha266_2635

Alternate gene names: 119358399

Gene position: 3025865-3023817 (Counterclockwise)

Preceding gene: 119358400

Following gene: 119358398

Centisome position: 96.55

GC content: 54.22

Gene sequence:

>2049_bases
GTGAAACGATATATCTTTTTATTCGGGAAGTACATTCTTGATTCGCCTCGCTATGTCAAGTGGCTGATAGCGGTTGGGTT
CGATCTGTTTTCGGCGCTCATAACGGTCTGGCTTGCCTGTTCGTTACGACTCGAATCGATCAGCCTGCCCGATCTCACGC
ACCTCAGGCTCTACCTGCTGGCGCCGGCACTTATGCTCCCGATATTTACCCTGTTTGGCCTCTATCATGCCATTCACCGG
TACAGTGGTTTTTCTGTTTTCATTACGGTCGGAAAGGCCATGCTGGTCTACGGATTTGCCTTTTTTTCCATCATGCTGTT
GATGCAGATCGACGGCATTCCCCGTTCGATCGGGATCCTGCAGCCGATTCTCCTCTTTCTGATGACGGCGGGCAGCCGGG
CCATGGTCAGGTTTATGTACACGGCGGCAACCTCGCACAGCATGAGAGGGCGTTCGCTCGACAGATTGTTGATCTACGGA
GCGGGCTCATCAGGGGCCGAGATAGGCAAGGCCTTGCAGGAACGGGCGAACTATGAACTGCTCGGCTATCTCGACGACAG
CAAGGAGCTGCACGGTCGCAAGCTGAACGGCATTCCGGTCTACTACCCCGGGGAGGCGGAGCGGCTCATTCAGAAGGAGG
GAATCAACAATGTGCTCATTGCCATGCCGTCGGCAAGCCGAACCCGCAGGAATGAGATTATCGAAAGTTTCCGCCGCTAT
CCGGTTCGCATTCAGACGCTTCCAGGCGTTGACGAGCTTGCGGATGGACGGGTAACCATATCGGATATCAAGGAGGTTGA
GATCGAGGATCTGCTCGGTCGCGACCCTGTTCCGATCGACCATGAACTGGTTCATCGGGCAATTACGAAAAAGGTGGTTC
TGGTAACGGGAGCGGGCGGAAGCATCGGCAGCGAACTCTGCCGTCAGCTTCTTCAGGCCCATCCGGCAACCCTTCTGCTG
CTTGACAATACCGAGCTCAATCTCTACACCATTCACTGCGATGTTGAGGCCCGGGCACAGAAAATCCAGGCGGCAACCCG
GTTTGTTCCACTGCTCTGCGATGTAACGGACGAGGAACGGATTACCGAAATATGCCGGGTTTTCAAGCCGGAAGTCATCT
ACCACGCCGCCGCCTACAAGCATGTGCCGATGGTTGAGTACAATCCTGCCGAGGGGGTTCGCAATAACGTTTTTGGTACG
CTGAGCGTTGTTCGGGCGGCAATCCGTCAGGGGGTTTCGAATTTTGTGCTGGTCAGTACCGACAAGGCCGTACGGCCAAC
CAATGTGATGGGTGCAAGCAAACGGCTCTGTGAAATGGCGCTGCAGGCGCATGCTGCAGAACAGGGGCACAAAACCTGTT
TTTCTATGGTTCGTTTCGGCAACGTTCTTGGTTCGAGCGGCTCGGTCGTACCGCTCTTCCGCAGGCAGATCAAGGAAGGA
AACGCGATAACCATCACGCATGAGGAGATCACCCGGTACTTCATGACCATACCCGAAGCCGCGCAGCTTATCATACAGGC
CGGAGCCATGGCAACGGGGGGCGACCTCTATCTGCTCGAAATGGGCAAACCCGTTAAAATCATTGACCTTGCCCGCAAGA
TGGTGGAGCTCTCCGGTCTCTCCGTGCGCGACCATGAAAACCCGAACGGCGATATCGAAATAAAGGTAACCGGCCTTCGT
CCCGGCGAAAAGCTCTACGAAGAGCTTCTCATCGGCAACAACTCCGGCCCGACGGCCAATCCCCGTATTTTCAAGGCGTA
TGAAGAGTTCATGCCCATTGGCGAACTCAATACCGAACTCTCGCAACTCATCGTAGCCATCAGGGTTAACGACGTAAAAA
ACATCAAGCGCATTCTCAATAAAATAATACCCGAATACAACCCGGCAGCCGAAACAACCGATCTCCTCGCATCGGAAACC
GAATGCCAGATCTGCGCCAACATCTCCCGCCCCAAAAAAGTCCAGAGCAATGGAAAAAACGGGAAAACTGTTAAAAGTGC
TGAGTTGGGGGTAATGGGTAATGGGAAGAGCGGGTCAAGAACGTGTTAA

Upstream 100 bases:

>100_bases
AGAGTAACAGAATCGGAAGTGCCGGCAGGCAGGTCGTCCTGAAAACGGGACGACCCCCTGTCCGATGCTCCGATAACGTT
CGACAAAACGGTTACAAGCA

Downstream 100 bases:

>100_bases
GTGCTACGGGTTACGTTGAAGAATGTAGGATGTGGGAGGATGGAAGTGCTGAGTGCTGAGCAATGAGTTCTGAATTGGGG
CAAAGAACAGAAGAGAGATC

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MKRYIFLFGKYILDSPRYVKWLIAVGFDLFSALITVWLACSLRLESISLPDLTHLRLYLLAPALMLPIFTLFGLYHAIHR
YSGFSVFITVGKAMLVYGFAFFSIMLLMQIDGIPRSIGILQPILLFLMTAGSRAMVRFMYTAATSHSMRGRSLDRLLIYG
AGSSGAEIGKALQERANYELLGYLDDSKELHGRKLNGIPVYYPGEAERLIQKEGINNVLIAMPSASRTRRNEIIESFRRY
PVRIQTLPGVDELADGRVTISDIKEVEIEDLLGRDPVPIDHELVHRAITKKVVLVTGAGGSIGSELCRQLLQAHPATLLL
LDNTELNLYTIHCDVEARAQKIQAATRFVPLLCDVTDEERITEICRVFKPEVIYHAAAYKHVPMVEYNPAEGVRNNVFGT
LSVVRAAIRQGVSNFVLVSTDKAVRPTNVMGASKRLCEMALQAHAAEQGHKTCFSMVRFGNVLGSSGSVVPLFRRQIKEG
NAITITHEEITRYFMTIPEAAQLIIQAGAMATGGDLYLLEMGKPVKIIDLARKMVELSGLSVRDHENPNGDIEIKVTGLR
PGEKLYEELLIGNNSGPTANPRIFKAYEEFMPIGELNTELSQLIVAIRVNDVKNIKRILNKIIPEYNPAAETTDLLASET
ECQICANISRPKKVQSNGKNGKTVKSAELGVMGNGKSGSRTC

Sequences:

>Translated_682_residues
MKRYIFLFGKYILDSPRYVKWLIAVGFDLFSALITVWLACSLRLESISLPDLTHLRLYLLAPALMLPIFTLFGLYHAIHR
YSGFSVFITVGKAMLVYGFAFFSIMLLMQIDGIPRSIGILQPILLFLMTAGSRAMVRFMYTAATSHSMRGRSLDRLLIYG
AGSSGAEIGKALQERANYELLGYLDDSKELHGRKLNGIPVYYPGEAERLIQKEGINNVLIAMPSASRTRRNEIIESFRRY
PVRIQTLPGVDELADGRVTISDIKEVEIEDLLGRDPVPIDHELVHRAITKKVVLVTGAGGSIGSELCRQLLQAHPATLLL
LDNTELNLYTIHCDVEARAQKIQAATRFVPLLCDVTDEERITEICRVFKPEVIYHAAAYKHVPMVEYNPAEGVRNNVFGT
LSVVRAAIRQGVSNFVLVSTDKAVRPTNVMGASKRLCEMALQAHAAEQGHKTCFSMVRFGNVLGSSGSVVPLFRRQIKEG
NAITITHEEITRYFMTIPEAAQLIIQAGAMATGGDLYLLEMGKPVKIIDLARKMVELSGLSVRDHENPNGDIEIKVTGLR
PGEKLYEELLIGNNSGPTANPRIFKAYEEFMPIGELNTELSQLIVAIRVNDVKNIKRILNKIIPEYNPAAETTDLLASET
ECQICANISRPKKVQSNGKNGKTVKSAELGVMGNGKSGSRTC
>Mature_682_residues
MKRYIFLFGKYILDSPRYVKWLIAVGFDLFSALITVWLACSLRLESISLPDLTHLRLYLLAPALMLPIFTLFGLYHAIHR
YSGFSVFITVGKAMLVYGFAFFSIMLLMQIDGIPRSIGILQPILLFLMTAGSRAMVRFMYTAATSHSMRGRSLDRLLIYG
AGSSGAEIGKALQERANYELLGYLDDSKELHGRKLNGIPVYYPGEAERLIQKEGINNVLIAMPSASRTRRNEIIESFRRY
PVRIQTLPGVDELADGRVTISDIKEVEIEDLLGRDPVPIDHELVHRAITKKVVLVTGAGGSIGSELCRQLLQAHPATLLL
LDNTELNLYTIHCDVEARAQKIQAATRFVPLLCDVTDEERITEICRVFKPEVIYHAAAYKHVPMVEYNPAEGVRNNVFGT
LSVVRAAIRQGVSNFVLVSTDKAVRPTNVMGASKRLCEMALQAHAAEQGHKTCFSMVRFGNVLGSSGSVVPLFRRQIKEG
NAITITHEEITRYFMTIPEAAQLIIQAGAMATGGDLYLLEMGKPVKIIDLARKMVELSGLSVRDHENPNGDIEIKVTGLR
PGEKLYEELLIGNNSGPTANPRIFKAYEEFMPIGELNTELSQLIVAIRVNDVKNIKRILNKIIPEYNPAAETTDLLASET
ECQICANISRPKKVQSNGKNGKTVKSAELGVMGNGKSGSRTC

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 75583; Mature: 75583

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYIFLFGKYILDSPRYVKWLIAVGFDLFSALITVWLACSLRLESISLPDLTHLRLYLL
CCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHH
APALMLPIFTLFGLYHAIHRYSGFSVFITVGKAMLVYGFAFFSIMLLMQIDGIPRSIGIL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QPILLFLMTAGSRAMVRFMYTAATSHSMRGRSLDRLLIYGAGSSGAEIGKALQERANYEL
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEE
LGYLDDSKELHGRKLNGIPVYYPGEAERLIQKEGINNVLIAMPSASRTRRNEIIESFRRY
EEEECCCHHHCCCCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
PVRIQTLPGVDELADGRVTISDIKEVEIEDLLGRDPVPIDHELVHRAITKKVVLVTGAGG
CEEEEECCCHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECCCC
SIGSELCRQLLQAHPATLLLLDNTELNLYTIHCDVEARAQKIQAATRFVPLLCDVTDEER
CHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHEECCCCHHH
ITEICRVFKPEVIYHAAAYKHVPMVEYNPAEGVRNNVFGTLSVVRAAIRQGVSNFVLVST
HHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DKAVRPTNVMGASKRLCEMALQAHAAEQGHKTCFSMVRFGNVLGSSGSVVPLFRRQIKEG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
NAITITHEEITRYFMTIPEAAQLIIQAGAMATGGDLYLLEMGKPVKIIDLARKMVELSGL
CEEEEEHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCC
SVRDHENPNGDIEIKVTGLRPGEKLYEELLIGNNSGPTANPRIFKAYEEFMPIGELNTEL
EEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
SQLIVAIRVNDVKNIKRILNKIIPEYNPAAETTDLLASETECQICANISRPKKVQSNGKN
HHHEEEHEECCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHCCCCCHHHHCCCCC
GKTVKSAELGVMGNGKSGSRTC
CCEEEECCEEEEECCCCCCCCC
>Mature Secondary Structure
MKRYIFLFGKYILDSPRYVKWLIAVGFDLFSALITVWLACSLRLESISLPDLTHLRLYLL
CCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHH
APALMLPIFTLFGLYHAIHRYSGFSVFITVGKAMLVYGFAFFSIMLLMQIDGIPRSIGIL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QPILLFLMTAGSRAMVRFMYTAATSHSMRGRSLDRLLIYGAGSSGAEIGKALQERANYEL
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEE
LGYLDDSKELHGRKLNGIPVYYPGEAERLIQKEGINNVLIAMPSASRTRRNEIIESFRRY
EEEECCCHHHCCCCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
PVRIQTLPGVDELADGRVTISDIKEVEIEDLLGRDPVPIDHELVHRAITKKVVLVTGAGG
CEEEEECCCHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECCCC
SIGSELCRQLLQAHPATLLLLDNTELNLYTIHCDVEARAQKIQAATRFVPLLCDVTDEER
CHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHEECCCCHHH
ITEICRVFKPEVIYHAAAYKHVPMVEYNPAEGVRNNVFGTLSVVRAAIRQGVSNFVLVST
HHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DKAVRPTNVMGASKRLCEMALQAHAAEQGHKTCFSMVRFGNVLGSSGSVVPLFRRQIKEG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
NAITITHEEITRYFMTIPEAAQLIIQAGAMATGGDLYLLEMGKPVKIIDLARKMVELSGL
CEEEEEHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCC
SVRDHENPNGDIEIKVTGLRPGEKLYEELLIGNNSGPTANPRIFKAYEEFMPIGELNTEL
EEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
SQLIVAIRVNDVKNIKRILNKIIPEYNPAAETTDLLASETECQICANISRPKKVQSNGKN
HHHEEEHEECCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHCCCCCHHHHCCCCC
GKTVKSAELGVMGNGKSGSRTC
CCEEEECCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]