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The map label for this gene is clpB1 [H]

Identifier: 119358345

GI number: 119358345

Start: 2951866

End: 2953188

Strand: Direct

Name: clpB1 [H]

Synonym: Cpha266_2577

Alternate gene names: 119358345

Gene position: 2951866-2953188 (Clockwise)

Preceding gene: 119358342

Following gene: 119358346

Centisome position: 94.19

GC content: 52.23

Gene sequence:

>1323_bases
ATGAGCATTGCTGATACCCGTCAACGGCTGCAGGAAATAGAAAAACAGCTTGCAAATCTTGTTGAGGAACAGACAACCAT
CAAAGCGCAATGGGATAGCGAAAAAGAGCTGATCCAGTCATCACGAACCCTCAAAGCAGAACTCGAGCAACTCCGCCTTC
AGGCCGAAGAGTTCGAACGACAGGGCGACTACGGCAAAGTAGCCGAAATCCGGTATGGAAAAATAGTGGATCTCCAGCGC
AGGATCGATGAAAACCGTGGAAAAATCGAAGAAAAAAAAGCATCCGGCGACCTCATCATGAAAGAAGAGATCGACGCTGA
AGATATCGCCGACATCGTCTCAAGATGGACAGGCATACCGGTCAGCAAAATGCTGCAATCCGAGCGACAGAAACTCCTGC
ACATCGAAACCGAACTTCACCAGCGGGTAATCGGCCAGGAAGAGGCCGTTACCGCGGTCAGCGAAGCGGTAAAGCGTTCA
AGAGCAGGAATGGGCGACGAAAAAAAACCAATCGGTTCATTTATCTTTCTCGGGCCCACAGGCGTAGGAAAAACCGAACT
TGCCCGTGCGCTTGCCGAGTACCTGTTCGACAACGAAGACTCGATGATCCGCATCGACATGAGCGAATACATGGAGTCGC
ATACCGTCAGCCGACTCGTCGGCGCCCCTCCCGGATATGTCGGCTATGAAGAGGGCGGACAGCTCACCGAAGCGGTCAGG
CGCAAACCATTCTCCGTCGTCCTGCTCGACGAAATCGAAAAAGCGCATCCCGACGTCTTCAACATCCTCCTGCAGATCCT
CGACGACGGCCGCCTCACCGACAACAAAGGACATACGGTGAACTTCAAGAACACCATCATCATCATGACCAGCAACATCG
GAGCCCAGCTCATCCAGGCAGAGATGGAAAAAATGGATGGCATGAGCCGCGACATGGCGCTGTCCGGTCTCCAGGAAAAA
CTCTTTCTTCTGCTCAAACAGCAGGTAAAACCGGAATTTCTCAACCGTATCGACGAAATCATCCTCTTTACCCCGCTCAG
CCGCACAGATCTCACAAAAATCGTGCTGATCCAGTTCAAACGCATACAGGAGATGGCGGCACGCCAGAGAATCACGCTTA
CCATCACAGACTCCGCGCTTGAATGGATAGCCCAGGCAGGATTCGACCCTGCTTTCGGAGCGCGACCGCTGAAACGGGTC
ATGCAGCGCAAAATCACCAACAAACTCTCGGAACTTATCCTCTCCGCAGAGGTTCAGGAAGACGACACCGTAGCCATCGA
TGTTGTGGATGGCGAACTGAAACTCACAAAAAAAATCGTCTAA

Upstream 100 bases:

>100_bases
TAATGATTGATGGAACGGCGAGGTTATCTTTTATGAAATTTATGGGTTAAATTTACACAATATTTTGTATTAACAGCCCG
CAATCAAACCAGACAAAACC

Downstream 100 bases:

>100_bases
AAAAAACAGCAAAAACACTCTCATATGAAAAGAAACCGATTCCCGCTCTCACTGCTGATCATGCTCTTGATGCTTCAGAC
CCCGGCTGCCTGGGCGGCAA

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSIADTRQRLQEIEKQLANLVEEQTTIKAQWDSEKELIQSSRTLKAELEQLRLQAEEFERQGDYGKVAEIRYGKIVDLQR
RIDENRGKIEEKKASGDLIMKEEIDAEDIADIVSRWTGIPVSKMLQSERQKLLHIETELHQRVIGQEEAVTAVSEAVKRS
RAGMGDEKKPIGSFIFLGPTGVGKTELARALAEYLFDNEDSMIRIDMSEYMESHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDNKGHTVNFKNTIIIMTSNIGAQLIQAEMEKMDGMSRDMALSGLQEK
LFLLLKQQVKPEFLNRIDEIILFTPLSRTDLTKIVLIQFKRIQEMAARQRITLTITDSALEWIAQAGFDPAFGARPLKRV
MQRKITNKLSELILSAEVQEDDTVAIDVVDGELKLTKKIV

Sequences:

>Translated_440_residues
MSIADTRQRLQEIEKQLANLVEEQTTIKAQWDSEKELIQSSRTLKAELEQLRLQAEEFERQGDYGKVAEIRYGKIVDLQR
RIDENRGKIEEKKASGDLIMKEEIDAEDIADIVSRWTGIPVSKMLQSERQKLLHIETELHQRVIGQEEAVTAVSEAVKRS
RAGMGDEKKPIGSFIFLGPTGVGKTELARALAEYLFDNEDSMIRIDMSEYMESHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDNKGHTVNFKNTIIIMTSNIGAQLIQAEMEKMDGMSRDMALSGLQEK
LFLLLKQQVKPEFLNRIDEIILFTPLSRTDLTKIVLIQFKRIQEMAARQRITLTITDSALEWIAQAGFDPAFGARPLKRV
MQRKITNKLSELILSAEVQEDDTVAIDVVDGELKLTKKIV
>Mature_439_residues
SIADTRQRLQEIEKQLANLVEEQTTIKAQWDSEKELIQSSRTLKAELEQLRLQAEEFERQGDYGKVAEIRYGKIVDLQRR
IDENRGKIEEKKASGDLIMKEEIDAEDIADIVSRWTGIPVSKMLQSERQKLLHIETELHQRVIGQEEAVTAVSEAVKRSR
AGMGDEKKPIGSFIFLGPTGVGKTELARALAEYLFDNEDSMIRIDMSEYMESHTVSRLVGAPPGYVGYEEGGQLTEAVRR
KPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDNKGHTVNFKNTIIIMTSNIGAQLIQAEMEKMDGMSRDMALSGLQEKL
FLLLKQQVKPEFLNRIDEIILFTPLSRTDLTKIVLIQFKRIQEMAARQRITLTITDSALEWIAQAGFDPAFGARPLKRVM
QRKITNKLSELILSAEVQEDDTVAIDVVDGELKLTKKIV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=327, Percent_Identity=37.3088685015291, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1788943, Length=431, Percent_Identity=48.7238979118329, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787109, Length=336, Percent_Identity=43.75, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=428, Percent_Identity=45.3271028037383, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323002, Length=426, Percent_Identity=39.6713615023474, Blast_Score=325, Evalue=1e-89,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49879; Mature: 49748

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIADTRQRLQEIEKQLANLVEEQTTIKAQWDSEKELIQSSRTLKAELEQLRLQAEEFER
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDYGKVAEIRYGKIVDLQRRIDENRGKIEEKKASGDLIMKEEIDAEDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHCCCC
VSKMLQSERQKLLHIETELHQRVIGQEEAVTAVSEAVKRSRAGMGDEKKPIGSFIFLGPT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARALAEYLFDNEDSMIRIDMSEYMESHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDNKGHTVNFKNTIIIMTSNIGAQLIQA
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHH
EMEKMDGMSRDMALSGLQEKLFLLLKQQVKPEFLNRIDEIILFTPLSRTDLTKIVLIQFK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RIQEMAARQRITLTITDSALEWIAQAGFDPAFGARPLKRVMQRKITNKLSELILSAEVQE
HHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DDTVAIDVVDGELKLTKKIV
CCEEEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SIADTRQRLQEIEKQLANLVEEQTTIKAQWDSEKELIQSSRTLKAELEQLRLQAEEFER
CCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDYGKVAEIRYGKIVDLQRRIDENRGKIEEKKASGDLIMKEEIDAEDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHCCCC
VSKMLQSERQKLLHIETELHQRVIGQEEAVTAVSEAVKRSRAGMGDEKKPIGSFIFLGPT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARALAEYLFDNEDSMIRIDMSEYMESHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDNKGHTVNFKNTIIIMTSNIGAQLIQA
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHH
EMEKMDGMSRDMALSGLQEKLFLLLKQQVKPEFLNRIDEIILFTPLSRTDLTKIVLIQFK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RIQEMAARQRITLTITDSALEWIAQAGFDPAFGARPLKRVMQRKITNKLSELILSAEVQE
HHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DDTVAIDVVDGELKLTKKIV
CCEEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]