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The map label for this gene is smaIR [H]

Identifier: 119358291

GI number: 119358291

Start: 2887701

End: 2888465

Strand: Direct

Name: smaIR [H]

Synonym: Cpha266_2523

Alternate gene names: 119358291

Gene position: 2887701-2888465 (Clockwise)

Preceding gene: 119358288

Following gene: 119358296

Centisome position: 92.14

GC content: 43.66

Gene sequence:

>765_bases
ATGAGCGAAATAGACAGAAACCGAATTGAATTGCTGGTTGCATCCATTCGGGAACTTACTCCGGGTCAATTTTCATGGCT
GGAGAGAACGGTTCAGATATTTCAGTGTGAGCATCACTACTCTATTTTGCATTCTGATCTCCTTGACGAAGAAACGCTGG
AGAATTTTGGTGATGCTCTGAGAATCCATCACAGTTTTTCGGTGGAACCATTTTCAAAGGATAAATTTGAATATGTGCTT
GAGCGTGTTGTCAACATGTCATCCGAGCGAGCAAAGCTTGCACCAAAAGGAAACCGAGGACATGATATCACCATAGACAA
TACCCGAGTTTCATTAAAAACACAGGCAGACAAAGGGATCAGGGAAGACAAAATATGGATCAGTAAATTTATGGAGCTGG
GCAAAGGGCACTGGGGAGATAAACCTGCTGATCTGGTATCGCTGCTCAACCTTTTTTTAACGCACCTTGACAACTATGAA
AGGATATTCATCCTCCGGGCTCTTCGGAAAGCACCAGACTGGATTTATGAACTGGTGGAAATACCAATGCAGTTGCTGTT
ACTTGCTGGCAGCGGAAAACTCGATATGAAAAGTGAAAGCAAGCAATTTCCGAAGCCAGGCTACTGCTTTGTAAATGAGG
GTGATACCGAGTTATACCGGCTATACTTCGATGGAGGAAGCGAGCGAAAGCTTCAGCTCAAAAATCTTCTTAAAAGTCGC
TGCATTGTGCATGCACGATGGGAATTTACGATTCCGGCAGTATAA

Upstream 100 bases:

>100_bases
AATTCAATTATTATAACTCACAACGCATTATTTGCATCTGATTCATGACTCTGTTGTTCATCTCTCTATTTGAAAAAAAC
ATCCCTGTAACCGTCGAATT

Downstream 100 bases:

>100_bases
GTGCGCCTTGTACACGGGCGTTGCGTCGGCTTGATATGTAAACATAATCCGGATTAATTTCAATACCGACACATTTTCTG
TTGAGTTCCTGCGAAACAAT

Product: Type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.SmaI; Endonuclease SmaI; Type II restriction enzyme SmaI [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSEIDRNRIELLVASIRELTPGQFSWLERTVQIFQCEHHYSILHSDLLDEETLENFGDALRIHHSFSVEPFSKDKFEYVL
ERVVNMSSERAKLAPKGNRGHDITIDNTRVSLKTQADKGIREDKIWISKFMELGKGHWGDKPADLVSLLNLFLTHLDNYE
RIFILRALRKAPDWIYELVEIPMQLLLLAGSGKLDMKSESKQFPKPGYCFVNEGDTELYRLYFDGGSERKLQLKNLLKSR
CIVHARWEFTIPAV

Sequences:

>Translated_254_residues
MSEIDRNRIELLVASIRELTPGQFSWLERTVQIFQCEHHYSILHSDLLDEETLENFGDALRIHHSFSVEPFSKDKFEYVL
ERVVNMSSERAKLAPKGNRGHDITIDNTRVSLKTQADKGIREDKIWISKFMELGKGHWGDKPADLVSLLNLFLTHLDNYE
RIFILRALRKAPDWIYELVEIPMQLLLLAGSGKLDMKSESKQFPKPGYCFVNEGDTELYRLYFDGGSERKLQLKNLLKSR
CIVHARWEFTIPAV
>Mature_253_residues
SEIDRNRIELLVASIRELTPGQFSWLERTVQIFQCEHHYSILHSDLLDEETLENFGDALRIHHSFSVEPFSKDKFEYVLE
RVVNMSSERAKLAPKGNRGHDITIDNTRVSLKTQADKGIREDKIWISKFMELGKGHWGDKPADLVSLLNLFLTHLDNYER
IFILRALRKAPDWIYELVEIPMQLLLLAGSGKLDMKSESKQFPKPGYCFVNEGDTELYRLYFDGGSERKLQLKNLLKSRC
IVHARWEFTIPAV

Specific function: Recognizes the double-stranded sequence CCCGGG and cleaves after C-3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 29552; Mature: 29421

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIDRNRIELLVASIRELTPGQFSWLERTVQIFQCEHHYSILHSDLLDEETLENFGDAL
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHE
RIHHSFSVEPFSKDKFEYVLERVVNMSSERAKLAPKGNRGHDITIDNTRVSLKTQADKGI
EEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEECCCEEEEEECCCCCC
REDKIWISKFMELGKGHWGDKPADLVSLLNLFLTHLDNYERIFILRALRKAPDWIYELVE
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
IPMQLLLLAGSGKLDMKSESKQFPKPGYCFVNEGDTELYRLYFDGGSERKLQLKNLLKSR
HHHHHHHHCCCCCCCCCCHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
CIVHARWEFTIPAV
CEEEEEEEEECCCC
>Mature Secondary Structure 
SEIDRNRIELLVASIRELTPGQFSWLERTVQIFQCEHHYSILHSDLLDEETLENFGDAL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHE
RIHHSFSVEPFSKDKFEYVLERVVNMSSERAKLAPKGNRGHDITIDNTRVSLKTQADKGI
EEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEECCCEEEEEECCCCCC
REDKIWISKFMELGKGHWGDKPADLVSLLNLFLTHLDNYERIFILRALRKAPDWIYELVE
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
IPMQLLLLAGSGKLDMKSESKQFPKPGYCFVNEGDTELYRLYFDGGSERKLQLKNLLKSR
HHHHHHHHCCCCCCCCCCHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHH
CIVHARWEFTIPAV
CEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690008 [H]