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The map label for this gene is tuf [H]

Identifier: 119358193

GI number: 119358193

Start: 2798984

End: 2800165

Strand: Reverse

Name: tuf [H]

Synonym: Cpha266_2425

Alternate gene names: 119358193

Gene position: 2800165-2798984 (Counterclockwise)

Preceding gene: 119358194

Following gene: 119358192

Centisome position: 89.35

GC content: 48.56

Gene sequence:

>1182_bases
ATGGCAAAAGAGTCTTACAAAAGGGATAAGCCTCATGTCAATATTGGTACTATCGGTCACGTTGATCATGGTAAAACAAC
CTTAACTGCGGCAATCACCTCGGTGCTTGCAAAGCAGGGACTTGCGCAACAGCGCGATTTCGGCAGTATCGATAAAGCGC
CGGAAGAAAGGGAACGCGGTATTACCATTTCAACGGCACACGTTGAGTACCAGACGAAAAAACGGCATTATGCGCACATT
GACTGTCCTGGCCACGCTGACTACATCAAGAATATGATTACCGGTGCAGCCCAGATGGACGGAGCAATTCTTGTTGTTGC
AGGTACCGATGGACCTATGCCTCAGACCCGCGAGCACATTCTGCTTGCCCGTCAGGTAAACGTTCCGGCTCTTGTTGTTT
ATCTCAACAAAGTCGACATCGCCGATCCTGAACTTATCGAGCTCGTTGAGCTTGAGTTGAGAGAGCTTTTGACAGAGTAC
AACTTTCCTGGCGACGATATTCCTATTATTAAGGGTTCAGCGCTGAAGGCTCTTGATGGAGATCTGGAAGGCGAAAAATC
TATCATGGAATTGATGGATGCGGTTGATGAGTTTATTCCCGAGCCGCTTCGCGATATCGACAAGCCGTTTCTAATGCCTG
TCGAGGATGTATTCTCCATTTCAGGACGCGGTACTGTAGGAACCGGAAGAATCGAGCGCGGACGCATCAAGATCAACGAG
GAAGTTGAGATTGTTGGTATCAAGCCGACTCGCAAATCCGTTGTTACCGGTATCGAGATGTTCCAGAAACTGCTTGATGA
AGGGCAGGCTGGCGATAACGCTGGTCTTCTGCTTCGCGGCGTCGATAAAACAGAGCTCGAGCGCGGTATGGTTATTGCCA
AGCCGGGTACGATCAAACCGCATACGAAGTTCAAGGCTGAGGTCTATATTCTGAGAAAAGAAGAGGGTGGACGTCACACT
CCGTTTTTCAACGGCTATCGTCCGCAGTTCTATTTCAGAACCACTGATGTTACCGGTTCAGTAACTTTGCCGGAAGGTGT
CGAAATGGTTATGCCTGGAGATAACCTCTCGGTTGATGTTGAGCTTATCGTTCCTATCGCCATGGATGAAAACCTTCGTT
TCGCTATTCGTGAAGGTGGACGCACTGTAGGTGCCGGTTCTGTAACAAAAATCATAGAGTAA

Upstream 100 bases:

>100_bases
TCGCTTCAGGAGAAAAGAGTGAGCAAGGAAACGGTATAGGACGGCTATTTTCAGTTTTAACGATCAAAATAATAACAGAT
AACCCAGAGGGAGATAAGTT

Downstream 100 bases:

>100_bases
TTGACTGATATGTCGTCCCGGGGCGGGGCTCAGTGAATCCCGCCCCTTTTTATTGCTAACAAGAAACAGGGTTGACAAGT
GGCTGCACAGCAAAAGATTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGLAQQRDFGSIDKAPEERERGITISTAHVEYQTKKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVYLNKVDIADPELIELVELELRELLTEY
NFPGDDIPIIKGSALKALDGDLEGEKSIMELMDAVDEFIPEPLRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKINE
EVEIVGIKPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILRKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIVPIAMDENLRFAIREGGRTVGAGSVTKIIE

Sequences:

>Translated_393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGLAQQRDFGSIDKAPEERERGITISTAHVEYQTKKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVYLNKVDIADPELIELVELELRELLTEY
NFPGDDIPIIKGSALKALDGDLEGEKSIMELMDAVDEFIPEPLRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKINE
EVEIVGIKPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILRKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIVPIAMDENLRFAIREGGRTVGAGSVTKIIE
>Mature_392_residues
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGLAQQRDFGSIDKAPEERERGITISTAHVEYQTKKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVYLNKVDIADPELIELVELELRELLTEYN
FPGDDIPIIKGSALKALDGDLEGEKSIMELMDAVDEFIPEPLRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKINEE
VEIVGIKPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILRKEEGGRHTP
FFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDVELIVPIAMDENLRFAIREGGRTVGAGSVTKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=467, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=31.4220183486239, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=31.4220183486239, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.1479820627803, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.2511210762332, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI54607086, Length=312, Percent_Identity=31.4102564102564, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=28.7833827893175, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=28.7833827893175, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=28.7833827893175, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=266, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI157426893, Length=224, Percent_Identity=29.9107142857143, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=35.8208955223881, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI18390331, Length=131, Percent_Identity=35.8778625954198, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=71.7557251908397, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=71.7557251908397, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=30.0791556728232, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994988, Length=242, Percent_Identity=33.4710743801653, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789108, Length=256, Percent_Identity=29.6875, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=34.0425531914894, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.3759398496241, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25141371, Length=370, Percent_Identity=44.3243243243243, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=30.9734513274336, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=30.9734513274336, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=30.607476635514, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=447, Percent_Identity=29.082774049217, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=447, Percent_Identity=29.082774049217, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=445, Percent_Identity=26.0674157303371, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=30.5369127516779, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=42.1768707482993, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=28.158844765343, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556745, Length=128, Percent_Identity=35.15625, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=173, Percent_Identity=35.2601156069364, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=66.4987405541562, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=30.0668151447661, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=30.0668151447661, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=24.3478260869565, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=27.6967930029155, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=28.6764705882353, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.0740740740741, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=56.3775510204082, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=56.3775510204082, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=47.5324675324675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=30.3769401330377, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=30.3769401330377, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=30.3769401330377, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=30.3769401330377, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=30.4932735426009, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=30.4932735426009, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=33.2236842105263, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45550900, Length=449, Percent_Identity=29.6213808463252, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=27.0893371757925, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=27.5, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=27.5, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78706572, Length=244, Percent_Identity=28.2786885245902, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28572034, Length=174, Percent_Identity=34.4827586206897, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=131, Percent_Identity=35.1145038167939, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574573, Length=140, Percent_Identity=37.1428571428571, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43289; Mature: 43158

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGLAQQRDFGSIDKAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHCC
ITISTAHVEYQTKKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVYLNKVDIADPELIELVELELRELLTEYNFPGDDIPIIKGSALKALDG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCCC
DLEGEKSIMELMDAVDEFIPEPLRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKINE
CCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGIKPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILRKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIVPIAMDENLRFAIREGGRTVGAGSVTKIIE
EEEEEEEECCCCEEEEECCCCEECCCCHHHHCC
>Mature Secondary Structure 
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGLAQQRDFGSIDKAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHCC
ITISTAHVEYQTKKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVYLNKVDIADPELIELVELELRELLTEYNFPGDDIPIIKGSALKALDG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCCC
DLEGEKSIMELMDAVDEFIPEPLRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKINE
CCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGIKPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILRKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSVDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIVPIAMDENLRFAIREGGRTVGAGSVTKIIE
EEEEEEEECCCCEEEEECCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA