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The map label for this gene is dnaK [H]

Identifier: 119358033

GI number: 119358033

Start: 2588324

End: 2589943

Strand: Reverse

Name: dnaK [H]

Synonym: Cpha266_2261

Alternate gene names: 119358033

Gene position: 2589943-2588324 (Counterclockwise)

Preceding gene: 119358034

Following gene: 119358032

Centisome position: 82.64

GC content: 61.17

Gene sequence:

>1620_bases
ATGGCTCTCGCTTTTGATTTCGGGACATGCAACAGCGTTGTGGCTCGCTGGAACGAAGCCCTGCATGCCGTGGAAACTCC
CGAACTGCCCAATCTCGGCATCAGGTTTCCGCAGCCGGGATCGGAACCCGCGCGGGTCATTCCCTCCGTCATTCATTTTG
CGGAAGATAACGGGCTCCTTGCCGGCAGCCAGGTCGGCGCTTCAGGTCTTGTCAACCATCCTGGAACCTTCAGGTGGCTG
AAAATGGATATGCTCCGCACCGGCGGAGCCAATCGCGGACGGCGCGTCAACGGCAGGGTTATCTACCCCCGCCATGCGGC
GGACGAGCTCGTCGATCGCATCCTGATGTTCGTGCGCGGCTATTTCGGCGACATCGACGAGGAGATCGTGCTGACCGTCC
CGGTGGAAGCCTTCGACCACTACATCGACTGGCTTCGCGAAGCGGCCATAAAACGGTTTCCGGGCGGCATCACCTTCATC
GACGAGGCAACCGCCTGCATGCTCGGGTACAGCGACGAGGTGCTCGACGGCGAAGCGTACTGCATCGTGGATTTCGGCGG
CGGCACCCTCGACGTTTCGATAGTCCGTACCGATCTGCTGCATGCCGGTCATACGAAATGCCGATTGCTTGGCCGGGCCG
GCGAGGAGATCGGCGGCATCATGGTCGATAACTGGCTGCTGGAACATATCCAGGAACGGGAGGGGCTCACGGACGACGAT
ATCGCCTCGATCGGCCCGTCCCTGCTCGAAGCGGTTGAGCAGGCGAAAATAGTCATCTCCAGCGGCGGTGAAGAGTCATC
GTTCACGCGCTATAACGACGTTACCGGCCGTCTTATCGAGGTCACCCTCACTGCCGGCACCCTGAAGGAGGTGCTCGAAA
AAAAACGGGAGCCCGGCAGGAACAACCTCTACCAGTACATCATCCGCACGCTCGACCGCGCCATCGATCAGGCGCGCGAC
CGGGCCGGTATGCGCAAGGAGGAGCTCAAGGGGGTTTTTCTTGTGGGTGGCAGCAGCCTGCTGCCGGGCATCGAGGAGAA
AATCCGGGAGTTTTTCCCCCGCTGCGACGTCCATGCAGGCAATCCGTTCGAAGCCATTGCCCGTGGCGCATGCCGCTTCT
CCGGCGGGATGTTCGACCAGACGCTGACGCACGATTACTGCCTGCGCAGCTGGAACCGGGAACTCCGGGATTTCGAACTG
GTTCCCGTTGTTGCGCGAGGCACCCCGTACCCGACCGGCAAACCGGTGTGCAGCAAGTATATCAAGGCCGCCTCGGACAA
TCAGGAGGCGCTCGGGCTGGTCATTTATGAGCGCTCTTCGATGATCCGTCCGCTGATCTCCTACGTCAACGGGGCCGACG
GCTTGCGTCCGGTACGGGAAGGGGAGCGAGAGGAGAGTCGGCATAAGCCCCTCAATCCCGAAGACAGCGAGTTTATCCAT
GCCGTACCGCCCTGCCGTTCTGGCGATCGGCGCTTTATTGCAGGATTCGGGGTGGACGGCAACCGCCGTCTCACGCTCTG
GCTGCGGGATACGGAGGAGAACAACCGTTCATACATCCTGTTGCGCGACGGCTCGCGACTTCCGTTGCCGGTGATGGATC
TGCCGGTGGTAAAACTGTGA

Upstream 100 bases:

>100_bases
ATCACCGTTGACAGCAATGACGACGCCATTGATAGAAATGCCGTGGAGACCGAGGAACTGACGGATGCGGAGTGGTAACC
CATGATAACCGTTTGAAGTA

Downstream 100 bases:

>100_bases
CGGGCATCCATAAAATTTATTGCGCGATGCGATTGCTGCGTTGTCCCGACAAGTCGGGATCGAAATCCTCATCCCGAACG
CTTTCGGGACTCCGGTTTCT

Product: DnaK like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MALAFDFGTCNSVVARWNEALHAVETPELPNLGIRFPQPGSEPARVIPSVIHFAEDNGLLAGSQVGASGLVNHPGTFRWL
KMDMLRTGGANRGRRVNGRVIYPRHAADELVDRILMFVRGYFGDIDEEIVLTVPVEAFDHYIDWLREAAIKRFPGGITFI
DEATACMLGYSDEVLDGEAYCIVDFGGGTLDVSIVRTDLLHAGHTKCRLLGRAGEEIGGIMVDNWLLEHIQEREGLTDDD
IASIGPSLLEAVEQAKIVISSGGEESSFTRYNDVTGRLIEVTLTAGTLKEVLEKKREPGRNNLYQYIIRTLDRAIDQARD
RAGMRKEELKGVFLVGGSSLLPGIEEKIREFFPRCDVHAGNPFEAIARGACRFSGGMFDQTLTHDYCLRSWNRELRDFEL
VPVVARGTPYPTGKPVCSKYIKAASDNQEALGLVIYERSSMIRPLISYVNGADGLRPVREGEREESRHKPLNPEDSEFIH
AVPPCRSGDRRFIAGFGVDGNRRLTLWLRDTEENNRSYILLRDGSRLPLPVMDLPVVKL

Sequences:

>Translated_539_residues
MALAFDFGTCNSVVARWNEALHAVETPELPNLGIRFPQPGSEPARVIPSVIHFAEDNGLLAGSQVGASGLVNHPGTFRWL
KMDMLRTGGANRGRRVNGRVIYPRHAADELVDRILMFVRGYFGDIDEEIVLTVPVEAFDHYIDWLREAAIKRFPGGITFI
DEATACMLGYSDEVLDGEAYCIVDFGGGTLDVSIVRTDLLHAGHTKCRLLGRAGEEIGGIMVDNWLLEHIQEREGLTDDD
IASIGPSLLEAVEQAKIVISSGGEESSFTRYNDVTGRLIEVTLTAGTLKEVLEKKREPGRNNLYQYIIRTLDRAIDQARD
RAGMRKEELKGVFLVGGSSLLPGIEEKIREFFPRCDVHAGNPFEAIARGACRFSGGMFDQTLTHDYCLRSWNRELRDFEL
VPVVARGTPYPTGKPVCSKYIKAASDNQEALGLVIYERSSMIRPLISYVNGADGLRPVREGEREESRHKPLNPEDSEFIH
AVPPCRSGDRRFIAGFGVDGNRRLTLWLRDTEENNRSYILLRDGSRLPLPVMDLPVVKL
>Mature_538_residues
ALAFDFGTCNSVVARWNEALHAVETPELPNLGIRFPQPGSEPARVIPSVIHFAEDNGLLAGSQVGASGLVNHPGTFRWLK
MDMLRTGGANRGRRVNGRVIYPRHAADELVDRILMFVRGYFGDIDEEIVLTVPVEAFDHYIDWLREAAIKRFPGGITFID
EATACMLGYSDEVLDGEAYCIVDFGGGTLDVSIVRTDLLHAGHTKCRLLGRAGEEIGGIMVDNWLLEHIQEREGLTDDDI
ASIGPSLLEAVEQAKIVISSGGEESSFTRYNDVTGRLIEVTLTAGTLKEVLEKKREPGRNNLYQYIIRTLDRAIDQARDR
AGMRKEELKGVFLVGGSSLLPGIEEKIREFFPRCDVHAGNPFEAIARGACRFSGGMFDQTLTHDYCLRSWNRELRDFELV
PVVARGTPYPTGKPVCSKYIKAASDNQEALGLVIYERSSMIRPLISYVNGADGLRPVREGEREESRHKPLNPEDSEFIHA
VPPCRSGDRRFIAGFGVDGNRRLTLWLRDTEENNRSYILLRDGSRLPLPVMDLPVVKL

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=458, Percent_Identity=24.4541484716157, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI16507237, Length=363, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786196, Length=477, Percent_Identity=26.2054507337526, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786870, Length=434, Percent_Identity=23.963133640553, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788875, Length=393, Percent_Identity=24.6819338422392, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17534771, Length=445, Percent_Identity=24.7191011235955, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507981, Length=329, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322505, Length=562, Percent_Identity=22.7758007117438, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322426, Length=355, Percent_Identity=24.2253521126761, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320805, Length=568, Percent_Identity=23.0633802816901, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319396, Length=362, Percent_Identity=22.9281767955801, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24653595, Length=467, Percent_Identity=23.982869379015, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 60010; Mature: 59879

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAFDFGTCNSVVARWNEALHAVETPELPNLGIRFPQPGSEPARVIPSVIHFAEDNGLL
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEE
AGSQVGASGLVNHPGTFRWLKMDMLRTGGANRGRRVNGRVIYPRHAADELVDRILMFVRG
ECCCCCCCCCCCCCCCEEHEEHHHHHCCCCCCCCEECCEEEECCHHHHHHHHHHHHHHHH
YFGDIDEEIVLTVPVEAFDHYIDWLREAAIKRFPGGITFIDEATACMLGYSDEVLDGEAY
HHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHEECCCCCEECCCEE
CIVDFGGGTLDVSIVRTDLLHAGHTKCRLLGRAGEEIGGIMVDNWLLEHIQEREGLTDDD
EEEEECCCEEEEEEEHHHHHHCCCHHHEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCH
IASIGPSLLEAVEQAKIVISSGGEESSFTRYNDVTGRLIEVTLTAGTLKEVLEKKREPGR
HHHCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCH
NNLYQYIIRTLDRAIDQARDRAGMRKEELKGVFLVGGSSLLPGIEEKIREFFPRCDVHAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHCCCHHHHHHHHCCCCCCCCC
NPFEAIARGACRFSGGMFDQTLTHDYCLRSWNRELRDFELVPVVARGTPYPTGKPVCSKY
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHH
IKAASDNQEALGLVIYERSSMIRPLISYVNGADGLRPVREGEREESRHKPLNPEDSEFIH
HHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCCCCCCCEEE
AVPPCRSGDRRFIAGFGVDGNRRLTLWLRDTEENNRSYILLRDGSRLPLPVMDLPVVKL
ECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALAFDFGTCNSVVARWNEALHAVETPELPNLGIRFPQPGSEPARVIPSVIHFAEDNGLL
EEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEE
AGSQVGASGLVNHPGTFRWLKMDMLRTGGANRGRRVNGRVIYPRHAADELVDRILMFVRG
ECCCCCCCCCCCCCCCEEHEEHHHHHCCCCCCCCEECCEEEECCHHHHHHHHHHHHHHHH
YFGDIDEEIVLTVPVEAFDHYIDWLREAAIKRFPGGITFIDEATACMLGYSDEVLDGEAY
HHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHEECCCCCEECCCEE
CIVDFGGGTLDVSIVRTDLLHAGHTKCRLLGRAGEEIGGIMVDNWLLEHIQEREGLTDDD
EEEEECCCEEEEEEEHHHHHHCCCHHHEEECCCCHHHCCCHHHHHHHHHHHHHCCCCCCH
IASIGPSLLEAVEQAKIVISSGGEESSFTRYNDVTGRLIEVTLTAGTLKEVLEKKREPGR
HHHCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCH
NNLYQYIIRTLDRAIDQARDRAGMRKEELKGVFLVGGSSLLPGIEEKIREFFPRCDVHAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHCCCHHHHHHHHCCCCCCCCC
NPFEAIARGACRFSGGMFDQTLTHDYCLRSWNRELRDFELVPVVARGTPYPTGKPVCSKY
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHH
IKAASDNQEALGLVIYERSSMIRPLISYVNGADGLRPVREGEREESRHKPLNPEDSEFIH
HHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCCCCCCCEEE
AVPPCRSGDRRFIAGFGVDGNRRLTLWLRDTEENNRSYILLRDGSRLPLPVMDLPVVKL
ECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA