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The map label for this gene is dagK [H]

Identifier: 119357831

GI number: 119357831

Start: 2358592

End: 2359521

Strand: Reverse

Name: dagK [H]

Synonym: Cpha266_2039

Alternate gene names: 119357831

Gene position: 2359521-2358592 (Counterclockwise)

Preceding gene: 119357832

Following gene: 119357830

Centisome position: 75.29

GC content: 49.35

Gene sequence:

>930_bases
ATGCCCAAACGTTTGTTTTATACTTTTATTCTCAATCCGGCGGCTGACAAGGGGCGCGCTGCCAGCAGGGCTGGATGGCT
GAAGGGGCTTGTTTCGCAACGAAAGGATGCTGTCTTCATGACGACGGCTTTTTCCGGACATGCGGAGGAGATCGCCCGAT
CGGCGAGTGCCGACAGTGCCTGTCTGGTTGCGTGCGGTGGTGATGGCACTCTTCATGAGATTGTCAACGCGGTAGTCGGA
ACGGATGTTGCTGTGGGTATTGTGCCAATCGGTTCTGCCAATGATTTTCTGAAAACATTTGAGCCTCGCCGCTGTGTGAA
GCAGGATCCCTCCTTTTCGTTCGGATCGGATACAAGGCTTGTTGATCTTGGCGGCGTGAGTGTTGGGAGCGGTGTGACGC
GATATTTTGTGAACTCTCTCGGCGTGGGATTGACCGGCCGCATTGCAAGGGTGGTAAAAAAAAACAGATGGCTGAAAGGC
GAGCTGGGCTACGTTCATGCGCTTCTGCGTGTGCTTGTAGGTTATAAACCGTTAAAAATGCATATTAAAATCACGCAGGA
TGACGCAATTATCGAGTTACATGAATCTGTTTTTGCCTTTTCAATATCGAACGGAAAAATTGAGGGCGGTAAATTTCTGA
TTGCTCCCGATGCTCTGCTGACAGACGGGTTGCTTGATATCTGTATTTTGAAATCCATACCGAAACTTGATTTTTTCCGG
TATGTCATCAAGTATATCAGGGGTACCCATATCAACGATACGAATGTTGTGTATTGTCAGGCCAGAGCGGTGGAGATTTT
TCTTTACGAGCCGGAGGTTATGCATCTGGACGGGGAGGTTTTTGAGGGCGTTCAGGGCTCTGTTCGCGTAGCAGTGCGTC
CTCTTGCGCTCAAGGTTCTTATGGATGCTTCAGGGCTTAATGTGGAATAA

Upstream 100 bases:

>100_bases
AGATTCTTTTGAGGATGTTTGATTATTATCCCGATCTTGCTCTGCAGGCCGAACCTTTCGAAACTTTTCAGCGTCAAGAG
CGATCAGGGGATTAACCGTT

Downstream 100 bases:

>100_bases
ACATGCAGATCATTATTTTTGAAGATGAACGGGTCTCTTTTCTGAAACCGCTCGCCAATCTTAAGCCCGTTTACGGACTG
GTTTCTGGTTTTTTGTCGCT

Product: diacylglycerol kinase, catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPKRLFYTFILNPAADKGRAASRAGWLKGLVSQRKDAVFMTTAFSGHAEEIARSASADSACLVACGGDGTLHEIVNAVVG
TDVAVGIVPIGSANDFLKTFEPRRCVKQDPSFSFGSDTRLVDLGGVSVGSGVTRYFVNSLGVGLTGRIARVVKKNRWLKG
ELGYVHALLRVLVGYKPLKMHIKITQDDAIIELHESVFAFSISNGKIEGGKFLIAPDALLTDGLLDICILKSIPKLDFFR
YVIKYIRGTHINDTNVVYCQARAVEIFLYEPEVMHLDGEVFEGVQGSVRVAVRPLALKVLMDASGLNVE

Sequences:

>Translated_309_residues
MPKRLFYTFILNPAADKGRAASRAGWLKGLVSQRKDAVFMTTAFSGHAEEIARSASADSACLVACGGDGTLHEIVNAVVG
TDVAVGIVPIGSANDFLKTFEPRRCVKQDPSFSFGSDTRLVDLGGVSVGSGVTRYFVNSLGVGLTGRIARVVKKNRWLKG
ELGYVHALLRVLVGYKPLKMHIKITQDDAIIELHESVFAFSISNGKIEGGKFLIAPDALLTDGLLDICILKSIPKLDFFR
YVIKYIRGTHINDTNVVYCQARAVEIFLYEPEVMHLDGEVFEGVQGSVRVAVRPLALKVLMDASGLNVE
>Mature_308_residues
PKRLFYTFILNPAADKGRAASRAGWLKGLVSQRKDAVFMTTAFSGHAEEIARSASADSACLVACGGDGTLHEIVNAVVGT
DVAVGIVPIGSANDFLKTFEPRRCVKQDPSFSFGSDTRLVDLGGVSVGSGVTRYFVNSLGVGLTGRIARVVKKNRWLKGE
LGYVHALLRVLVGYKPLKMHIKITQDDAIIELHESVFAFSISNGKIEGGKFLIAPDALLTDGLLDICILKSIPKLDFFRY
VIKYIRGTHINDTNVVYCQARAVEIFLYEPEVMHLDGEVFEGVQGSVRVAVRPLALKVLMDASGLNVE

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33560; Mature: 33429

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRLFYTFILNPAADKGRAASRAGWLKGLVSQRKDAVFMTTAFSGHAEEIARSASADSA
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCE
CLVACGGDGTLHEIVNAVVGTDVAVGIVPIGSANDFLKTFEPRRCVKQDPSFSFGSDTRL
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCCCCCCCEE
VDLGGVSVGSGVTRYFVNSLGVGLTGRIARVVKKNRWLKGELGYVHALLRVLVGYKPLKM
EEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEE
HIKITQDDAIIELHESVFAFSISNGKIEGGKFLIAPDALLTDGLLDICILKSIPKLDFFR
EEEECCCCCEEEEECCEEEEEECCCEECCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHH
YVIKYIRGTHINDTNVVYCQARAVEIFLYEPEVMHLDGEVFEGVQGSVRVAVRPLALKVL
HHHHHHCCCCCCCCCEEEEEEEEEEEEEECCEEEEECCHHHCCCCCCEEEEEHHEEEEEE
MDASGLNVE
ECCCCCCCC
>Mature Secondary Structure 
PKRLFYTFILNPAADKGRAASRAGWLKGLVSQRKDAVFMTTAFSGHAEEIARSASADSA
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCE
CLVACGGDGTLHEIVNAVVGTDVAVGIVPIGSANDFLKTFEPRRCVKQDPSFSFGSDTRL
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCCCCCCCEE
VDLGGVSVGSGVTRYFVNSLGVGLTGRIARVVKKNRWLKGELGYVHALLRVLVGYKPLKM
EEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEE
HIKITQDDAIIELHESVFAFSISNGKIEGGKFLIAPDALLTDGLLDICILKSIPKLDFFR
EEEECCCCCEEEEECCEEEEEECCCEECCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHH
YVIKYIRGTHINDTNVVYCQARAVEIFLYEPEVMHLDGEVFEGVQGSVRVAVRPLALKVL
HHHHHHCCCCCCCCCEEEEEEEEEEEEEECCEEEEECCHHHCCCCCCEEEEEHHEEEEEE
MDASGLNVE
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]