Click here to switch to the map view.

The map label for this gene is yuxG [H]

Identifier: 119357796

GI number: 119357796

Start: 2317575

End: 2319692

Strand: Reverse

Name: yuxG [H]

Synonym: Cpha266_2003

Alternate gene names: 119357796

Gene position: 2319692-2317575 (Counterclockwise)

Preceding gene: 119357797

Following gene: 119357795

Centisome position: 74.02

GC content: 49.91

Gene sequence:

>2118_bases
ATGCTGAACCTTTGGAACGACAATGATTTTCAGCGTTTTGTGCAGGCGCAGAAAAACGTGAACGACACGACTTCAGAACT
TGCGGAACTGGTCTACGCTTCCCGGCTGCTTGGGAGGGAGAGCTCTCTGGTGATGCATGGCGGCGGTAATACTTCGGTAA
AAAATGACCTGCACGATATTATCGGCAATACTGTCAATGTTATTTACATCAAAGGCAGCGGCATTGATCTTGCTGATGTC
GAGGCTTATGACTTTACGGCGGTCCGTCTGGAGCCGTTGCGGAAGCTTCAGCATCTCTATGCAACGGGGGAGAGGCGGAG
CGACGAGGATATTCGCAGGTTTTCGACAAGCGAATTCAAGCATTTTCTCTATCTGAATCTCTTTCAGCTCACGGATCACA
TTGTGAGCAATTCGCTTTCGCCCTCAATTGAGACGCTGTTGCATGCGTTTCTTCCGCACCGCTATATTTTTCATACGCAC
TCGTTGGCGTTGCTTACTCTGAGCAATCAGGCGGATGGCGGGGCGCGGGTTCGCGATGAGTTTGGCGATGAGTTTGGTTA
TCTCCCCTATATTCAACCCGGTCTTGGTCTCGCTCGTTCGGCCGCTGATGCATACAGGGTGAATCCTGAAATTTGCGGTC
TTGTTCTGCAAAAGCATGGTCTTGTTACGTTTGGCGCCAGTGCCAAAGAGGCATATATTCGTATGATCGAGCATGTTACC
AGACTTGAGGATTGTATCGCGAGAGCTGCAAGGAAAACGCGTATTCCGGTAACTCTTCCTGAAGAGATTGCTCTGCCTGA
GGATGTTGCTCCCGTTATCAGGGGGGCTGTGGTAGAGGAAACTGCGCCGGGTATACGTCAGTACCATCAGTTTATTCTTG
ATTTCAGAACATCTCCGGCTATTCTTGATTATGTCAATAGTCCCGATCTTTCGGCGATGAGCAGTAAAGGTGCTATGACT
CCGGATTTTATCATTCGCACAAAAAATCAGCCGCTTGTGCTTCAGGCTCCCGATGCACGGGATATTGAGGGCTTCAAGCT
CAGTGTTCAAGAAGCCGTACAGCGCTATCAGGCGAATTATTCGGCTTATTTCGATCGCCAGAAAGCTGCCAGTGCAATGA
ACGTATCCATGCTTGATTCGATGCCAAGGGTCGTTCTCGTGCCTGGATTGGGTCTTTTCGGTCTTGGCAAGACAGCAAAA
GCGGCCAGGATCAATGCTGATATTGCCGCAAGTACCGCTGTGGCTATTCTTGACGCCGAGTCAATCGGAGAGTTTGAGTC
CATTTCTGAATCCGAGGCATTTGCCATCGAGTATTGGGATATGGAGCAGTCCAAAGTTAACAAGGTTCGTCACGATGTAT
TCGCAGGTAAAGTTGTGCTTGTTACCGGAGCTGCCAGCGGGATCGGGCTTGCTACGGCTAAGGCTTTCCGCCAGAAAGGC
GCTGAACTGGTTATTCTCGATCTGAGTTATGATGCGCTTGAAAAGGCCAGGGAGGCTCTTGGAGAGAGTACGCTTGCAAT
AACTTGCGATGTTACCAACCGTGCGGCGGTGAAAAGCGCATTTGATCTTGCCTGTCGTACGTTTGGGGGTGTTGATATCA
TGGTTTCCAATGTCGGAGTCGCTTTACAGGGGAGAATCGGCGAGGTTTCTGACGAACTGCTTCGAAAGAGTTTTGAACTT
AATTTTTTTTCGCATCAGTCCATTGCCCAGGAGGCGGTGAGAGTAATGAAATTGCAGGGAACCGGCGGGGTTCTTCTTTT
CAATGTATCCAAACAGGCCGTCAACCCGGGAGCTGATTTTGGTCCTTATGGTCTTCCGAAAGCAGCGACGCTTTTTCTTG
TTCGCCAGTATGCGCTTGACCATGGACGCGATGGTATTCGCTCAAACGGCGTGAATGCAGACCGTATTCGAAGTGGTCTT
TTGACGGAGGAGATGATCAAGACCCGTTCACAGGCTCGCGGTCTGAGTGAAAAGGAGTATATGGCGGGAAATCTGCTTCA
GCTTGAAGTAACAGCCGAGGATGTTGCCGATGCTTTCGTTCATCTGGCGCTTGAAATCAGGACAACAGGGTCGATAACGA
CAGTGGACGGTGGAAATATTGCAGCCGCACTTCGATAA

Upstream 100 bases:

>100_bases
AACAACAATCATAACAAGTATGCCTGGCGCGTTATGCGGCACACCGGAACAATCTTCATAACATAACTCCTTCAAACAGG
ATAAGAAGTAATAGTTTTCT

Downstream 100 bases:

>100_bases
TAGTCTATTAAACATACCAACAACCATTGAAACAGGAGCGTATCAGTATGGCAGAATATGATAAGGATAAGCAGGCTAAA
GAGTATTATCTCGATACTCC

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MLNLWNDNDFQRFVQAQKNVNDTTSELAELVYASRLLGRESSLVMHGGGNTSVKNDLHDIIGNTVNVIYIKGSGIDLADV
EAYDFTAVRLEPLRKLQHLYATGERRSDEDIRRFSTSEFKHFLYLNLFQLTDHIVSNSLSPSIETLLHAFLPHRYIFHTH
SLALLTLSNQADGGARVRDEFGDEFGYLPYIQPGLGLARSAADAYRVNPEICGLVLQKHGLVTFGASAKEAYIRMIEHVT
RLEDCIARAARKTRIPVTLPEEIALPEDVAPVIRGAVVEETAPGIRQYHQFILDFRTSPAILDYVNSPDLSAMSSKGAMT
PDFIIRTKNQPLVLQAPDARDIEGFKLSVQEAVQRYQANYSAYFDRQKAASAMNVSMLDSMPRVVLVPGLGLFGLGKTAK
AARINADIAASTAVAILDAESIGEFESISESEAFAIEYWDMEQSKVNKVRHDVFAGKVVLVTGAASGIGLATAKAFRQKG
AELVILDLSYDALEKAREALGESTLAITCDVTNRAAVKSAFDLACRTFGGVDIMVSNVGVALQGRIGEVSDELLRKSFEL
NFFSHQSIAQEAVRVMKLQGTGGVLLFNVSKQAVNPGADFGPYGLPKAATLFLVRQYALDHGRDGIRSNGVNADRIRSGL
LTEEMIKTRSQARGLSEKEYMAGNLLQLEVTAEDVADAFVHLALEIRTTGSITTVDGGNIAAALR

Sequences:

>Translated_705_residues
MLNLWNDNDFQRFVQAQKNVNDTTSELAELVYASRLLGRESSLVMHGGGNTSVKNDLHDIIGNTVNVIYIKGSGIDLADV
EAYDFTAVRLEPLRKLQHLYATGERRSDEDIRRFSTSEFKHFLYLNLFQLTDHIVSNSLSPSIETLLHAFLPHRYIFHTH
SLALLTLSNQADGGARVRDEFGDEFGYLPYIQPGLGLARSAADAYRVNPEICGLVLQKHGLVTFGASAKEAYIRMIEHVT
RLEDCIARAARKTRIPVTLPEEIALPEDVAPVIRGAVVEETAPGIRQYHQFILDFRTSPAILDYVNSPDLSAMSSKGAMT
PDFIIRTKNQPLVLQAPDARDIEGFKLSVQEAVQRYQANYSAYFDRQKAASAMNVSMLDSMPRVVLVPGLGLFGLGKTAK
AARINADIAASTAVAILDAESIGEFESISESEAFAIEYWDMEQSKVNKVRHDVFAGKVVLVTGAASGIGLATAKAFRQKG
AELVILDLSYDALEKAREALGESTLAITCDVTNRAAVKSAFDLACRTFGGVDIMVSNVGVALQGRIGEVSDELLRKSFEL
NFFSHQSIAQEAVRVMKLQGTGGVLLFNVSKQAVNPGADFGPYGLPKAATLFLVRQYALDHGRDGIRSNGVNADRIRSGL
LTEEMIKTRSQARGLSEKEYMAGNLLQLEVTAEDVADAFVHLALEIRTTGSITTVDGGNIAAALR
>Mature_705_residues
MLNLWNDNDFQRFVQAQKNVNDTTSELAELVYASRLLGRESSLVMHGGGNTSVKNDLHDIIGNTVNVIYIKGSGIDLADV
EAYDFTAVRLEPLRKLQHLYATGERRSDEDIRRFSTSEFKHFLYLNLFQLTDHIVSNSLSPSIETLLHAFLPHRYIFHTH
SLALLTLSNQADGGARVRDEFGDEFGYLPYIQPGLGLARSAADAYRVNPEICGLVLQKHGLVTFGASAKEAYIRMIEHVT
RLEDCIARAARKTRIPVTLPEEIALPEDVAPVIRGAVVEETAPGIRQYHQFILDFRTSPAILDYVNSPDLSAMSSKGAMT
PDFIIRTKNQPLVLQAPDARDIEGFKLSVQEAVQRYQANYSAYFDRQKAASAMNVSMLDSMPRVVLVPGLGLFGLGKTAK
AARINADIAASTAVAILDAESIGEFESISESEAFAIEYWDMEQSKVNKVRHDVFAGKVVLVTGAASGIGLATAKAFRQKG
AELVILDLSYDALEKAREALGESTLAITCDVTNRAAVKSAFDLACRTFGGVDIMVSNVGVALQGRIGEVSDELLRKSFEL
NFFSHQSIAQEAVRVMKLQGTGGVLLFNVSKQAVNPGADFGPYGLPKAATLFLVRQYALDHGRDGIRSNGVNADRIRSGL
LTEEMIKTRSQARGLSEKEYMAGNLLQLEVTAEDVADAFVHLALEIRTTGSITTVDGGNIAAALR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG3347

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=226, Percent_Identity=29.646017699115, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI126723191, Length=209, Percent_Identity=28.7081339712919, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI40807363, Length=122, Percent_Identity=36.0655737704918, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI31542939, Length=221, Percent_Identity=28.5067873303167, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=31.0734463276836, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=30.4, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082100, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790717, Length=203, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1789378, Length=188, Percent_Identity=30.3191489361702, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=25.4980079681275, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=200, Percent_Identity=33, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=198, Percent_Identity=31.3131313131313, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=269, Percent_Identity=30.4832713754647, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=202, Percent_Identity=32.1782178217822, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=31.5, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=199, Percent_Identity=29.6482412060301, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=200, Percent_Identity=29, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=199, Percent_Identity=30.1507537688442, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=222, Percent_Identity=28.3783783783784, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=28.030303030303, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=202, Percent_Identity=29.7029702970297, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=202, Percent_Identity=30.6930693069307, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=28.1954887218045, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=202, Percent_Identity=29.2079207920792, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24663911, Length=141, Percent_Identity=37.5886524822695, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571387, Length=240, Percent_Identity=23.3333333333333, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013454
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short; PF00596 Aldolase_II [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 77052; Mature: 77052

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLWNDNDFQRFVQAQKNVNDTTSELAELVYASRLLGRESSLVMHGGGNTSVKNDLHDI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
IGNTVNVIYIKGSGIDLADVEAYDFTAVRLEPLRKLQHLYATGERRSDEDIRRFSTSEFK
CCCCEEEEEEECCCCCEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HFLYLNLFQLTDHIVSNSLSPSIETLLHAFLPHRYIFHTHSLALLTLSNQADGGARVRDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHH
FGDEFGYLPYIQPGLGLARSAADAYRVNPEICGLVLQKHGLVTFGASAKEAYIRMIEHVT
HCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
RLEDCIARAARKTRIPVTLPEEIALPEDVAPVIRGAVVEETAPGIRQYHQFILDFRTSPA
HHHHHHHHHHHHCCCCEECCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
ILDYVNSPDLSAMSSKGAMTPDFIIRTKNQPLVLQAPDARDIEGFKLSVQEAVQRYQANY
HEECCCCCCCHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEHHHHHHHHHCCH
SAYFDRQKAASAMNVSMLDSMPRVVLVPGLGLFGLGKTAKAARINADIAASTAVAILDAE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCHHCCCCCHHHHHEEEEEEHH
SIGEFESISESEAFAIEYWDMEQSKVNKVRHDVFAGKVVLVTGAASGIGLATAKAFRQKG
HCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCC
AELVILDLSYDALEKAREALGESTLAITCDVTNRAAVKSAFDLACRTFGGVDIMVSNVGV
CEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCE
ALQGRIGEVSDELLRKSFELNFFSHQSIAQEAVRVMKLQGTGGVLLFNVSKQAVNPGADF
EEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCHHHCCCCCCC
GPYGLPKAATLFLVRQYALDHGRDGIRSNGVNADRIRSGLLTEEMIKTRSQARGLSEKEY
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCHHHH
MAGNLLQLEVTAEDVADAFVHLALEIRTTGSITTVDGGNIAAALR
HCCCEEEEEECHHHHHHHHHHEEEEEEECCCEEEECCCCEEEECC
>Mature Secondary Structure
MLNLWNDNDFQRFVQAQKNVNDTTSELAELVYASRLLGRESSLVMHGGGNTSVKNDLHDI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
IGNTVNVIYIKGSGIDLADVEAYDFTAVRLEPLRKLQHLYATGERRSDEDIRRFSTSEFK
CCCCEEEEEEECCCCCEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HFLYLNLFQLTDHIVSNSLSPSIETLLHAFLPHRYIFHTHSLALLTLSNQADGGARVRDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHH
FGDEFGYLPYIQPGLGLARSAADAYRVNPEICGLVLQKHGLVTFGASAKEAYIRMIEHVT
HCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
RLEDCIARAARKTRIPVTLPEEIALPEDVAPVIRGAVVEETAPGIRQYHQFILDFRTSPA
HHHHHHHHHHHHCCCCEECCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
ILDYVNSPDLSAMSSKGAMTPDFIIRTKNQPLVLQAPDARDIEGFKLSVQEAVQRYQANY
HEECCCCCCCHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEHHHHHHHHHCCH
SAYFDRQKAASAMNVSMLDSMPRVVLVPGLGLFGLGKTAKAARINADIAASTAVAILDAE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCHHCCCCCHHHHHEEEEEEHH
SIGEFESISESEAFAIEYWDMEQSKVNKVRHDVFAGKVVLVTGAASGIGLATAKAFRQKG
HCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCC
AELVILDLSYDALEKAREALGESTLAITCDVTNRAAVKSAFDLACRTFGGVDIMVSNVGV
CEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCE
ALQGRIGEVSDELLRKSFELNFFSHQSIAQEAVRVMKLQGTGGVLLFNVSKQAVNPGADF
EEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCHHHCCCCCCC
GPYGLPKAATLFLVRQYALDHGRDGIRSNGVNADRIRSGLLTEEMIKTRSQARGLSEKEY
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCHHHH
MAGNLLQLEVTAEDVADAFVHLALEIRTTGSITTVDGGNIAAALR
HCCCEEEEEECHHHHHHHHHHEEEEEEECCCEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 8188684 [H]