Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 119357782

GI number: 119357782

Start: 2302346

End: 2303839

Strand: Direct

Name: purF [H]

Synonym: Cpha266_1989

Alternate gene names: 119357782

Gene position: 2302346-2303839 (Clockwise)

Preceding gene: 119357779

Following gene: 119357799

Centisome position: 73.47

GC content: 47.86

Gene sequence:

>1494_bases
ATGTGTGGAGTTTTCGGCATTTTCAACTCAAAAACTCCCGCTGAAGATACATTCTATGGGCTTTATTCCCTTCAGCACCG
GGGACAGGAAGCCGCCGGGATTGTTGTTGCAGAATACAACAAGGCGAAAAAGAAAACCCTTTTCAGGCAGCATAAGGGCA
TGGGTCTTGTTTCCGAGGTTTTCAAGGATGAAACCCTTTTTGAAAATCTGACGGGATATGCCGCAATCGGCCACAATCGA
TACTCAACCTCAGGCTCTTCCAAGTCAACAAACAACATCCAGCCCTTTTCTTTAACCTATCGTTCCGGCAGCCTTGCCAT
AGCACATAACGGCAACCTTACCAATTCACGCGCCCTGCGGCGCGAGCTGACGGAACTCGGAGTTATTTTTCAGGCATCGT
CAGATACTGAAATCATTCCTCACCTTGCGGCAAGAAGTCGCGAGAAAGAGCCTGTTAACCAGATTTACGAAGCTCTCAGA
CAGGTACAGGGAGCATACTCCATGGTCATTCTGGCCAATAACCAGTTGATTGCCGCACGAGATCCCTACGGATTCCGTCC
CCTTGCTCTCGGTAAGAAAACAGACCCGCTCACCGGTGAGCTCTCCTATATTGTGGCAAGCGAAACGTGCGCGTTCGACA
TCATCCAGGCCGAATACATCCGCGATATCGAACCTGGCGAAATTCTCCTGATCGATCATCTGGCCACGGCAAACGAAAAG
ACTTCATCCATTTTTCTGCCGCCCTCCAAACGCAAAGCCCGCTGCATCTTCGAGTATGTCTATTTTGCCCGTCCCGACAG
CTTTATCTTCAAGCACTCTGTTGACAAAGTCCGTCGAAATCTCGGCAAAAATCTCGCAAGAGAGTCAACGACAAAACAGA
ATCCTGACGAAAAAGAATTAACCGTTGTCAGTGTGCCTGACTCGTCAAATACAGCAGCTCTCGGCTTTGTCAGAGAAAGC
CTTAAAATGCAGCGGCCTGCCCGTTTTGAACATGGACTGATCCGCAATCACTATGTAGGAAGAACCTTCATTCAACCGGG
CATTCACAGTCGAGATATCAAGGTTAGATCAAAATACAATATTGTGCGCGGCGTGCTTTTCGACCGCCCGATCATTCTTA
TTGACGACTCGATTGTACGCGGTACAACGGCAAGAATGCTTATCAAACTGATCCGTGAAGCAGAGCCAAAATCCATCCAT
CTGCATATCAGCTCGCCGCCTATCACCAATCCCTGTTTTTACGGCATGGATTTTCCTACGAAAAGACAACTGCTGACGCA
CATGTTCGACAGCATTGACAATGAAGAAGAGGAAATAGAAAAAATAAGAGAGTATATCGGGGTTGATTCTCTCAAGTATC
TCTCCATGCAGGGTCTGTTGAACAGTGTTCCCTCTTTTGAAGATGAAACATGCAGTTACTGTACAGCCTGTTTCAGCGGC
GATTACCCGATCCAGGTTACCGATGTAACCACGGACAAGGAAGAAAACGACTGA

Upstream 100 bases:

>100_bases
TTTCTTGTTAGATTATTGGTTTCCTTGCCTGAAATATGTACCTTCAATAGCCATTTTTACCAGGCAAGCGAGTTTACACA
ATCTTAACGTGAGAGCGCAT

Downstream 100 bases:

>100_bases
AGGCGCAAAAAAAGGGGGCTATCGAAGGCCCCCTTTCAGTTTTCATCCATGAATCAATCACAAGCCGGAGTGCTATTTTT
TCACCTGCTTGAACTCGATT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFRQHKGMGLVSEVFKDETLFENLTGYAAIGHNR
YSTSGSSKSTNNIQPFSLTYRSGSLAIAHNGNLTNSRALRRELTELGVIFQASSDTEIIPHLAARSREKEPVNQIYEALR
QVQGAYSMVILANNQLIAARDPYGFRPLALGKKTDPLTGELSYIVASETCAFDIIQAEYIRDIEPGEILLIDHLATANEK
TSSIFLPPSKRKARCIFEYVYFARPDSFIFKHSVDKVRRNLGKNLARESTTKQNPDEKELTVVSVPDSSNTAALGFVRES
LKMQRPARFEHGLIRNHYVGRTFIQPGIHSRDIKVRSKYNIVRGVLFDRPIILIDDSIVRGTTARMLIKLIREAEPKSIH
LHISSPPITNPCFYGMDFPTKRQLLTHMFDSIDNEEEEIEKIREYIGVDSLKYLSMQGLLNSVPSFEDETCSYCTACFSG
DYPIQVTDVTTDKEEND

Sequences:

>Translated_497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFRQHKGMGLVSEVFKDETLFENLTGYAAIGHNR
YSTSGSSKSTNNIQPFSLTYRSGSLAIAHNGNLTNSRALRRELTELGVIFQASSDTEIIPHLAARSREKEPVNQIYEALR
QVQGAYSMVILANNQLIAARDPYGFRPLALGKKTDPLTGELSYIVASETCAFDIIQAEYIRDIEPGEILLIDHLATANEK
TSSIFLPPSKRKARCIFEYVYFARPDSFIFKHSVDKVRRNLGKNLARESTTKQNPDEKELTVVSVPDSSNTAALGFVRES
LKMQRPARFEHGLIRNHYVGRTFIQPGIHSRDIKVRSKYNIVRGVLFDRPIILIDDSIVRGTTARMLIKLIREAEPKSIH
LHISSPPITNPCFYGMDFPTKRQLLTHMFDSIDNEEEEIEKIREYIGVDSLKYLSMQGLLNSVPSFEDETCSYCTACFSG
DYPIQVTDVTTDKEEND
>Mature_497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFRQHKGMGLVSEVFKDETLFENLTGYAAIGHNR
YSTSGSSKSTNNIQPFSLTYRSGSLAIAHNGNLTNSRALRRELTELGVIFQASSDTEIIPHLAARSREKEPVNQIYEALR
QVQGAYSMVILANNQLIAARDPYGFRPLALGKKTDPLTGELSYIVASETCAFDIIQAEYIRDIEPGEILLIDHLATANEK
TSSIFLPPSKRKARCIFEYVYFARPDSFIFKHSVDKVRRNLGKNLARESTTKQNPDEKELTVVSVPDSSNTAALGFVRES
LKMQRPARFEHGLIRNHYVGRTFIQPGIHSRDIKVRSKYNIVRGVLFDRPIILIDDSIVRGTTARMLIKLIREAEPKSIH
LHISSPPITNPCFYGMDFPTKRQLLTHMFDSIDNEEEEIEKIREYIGVDSLKYLSMQGLLNSVPSFEDETCSYCTACFSG
DYPIQVTDVTTDKEEND

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=535, Percent_Identity=36.0747663551402, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI4826742, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788651, Length=502, Percent_Identity=36.0557768924303, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1790167, Length=196, Percent_Identity=29.0816326530612, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=510, Percent_Identity=35.4901960784314, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17532899, Length=219, Percent_Identity=26.9406392694064, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532897, Length=219, Percent_Identity=26.9406392694064, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539970, Length=219, Percent_Identity=26.027397260274, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=502, Percent_Identity=32.8685258964143, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28573187, Length=516, Percent_Identity=37.7906976744186, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24659598, Length=508, Percent_Identity=38.3858267716535, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24659604, Length=484, Percent_Identity=38.8429752066116, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI21357745, Length=212, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55993; Mature: 55993

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFRQHKGMGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHHCCCCHHHHH
FKDETLFENLTGYAAIGHNRYSTSGSSKSTNNIQPFSLTYRSGSLAIAHNGNLTNSRALR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
RELTELGVIFQASSDTEIIPHLAARSREKEPVNQIYEALRQVQGAYSMVILANNQLIAAR
HHHHHHCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
DPYGFRPLALGKKTDPLTGELSYIVASETCAFDIIQAEYIRDIEPGEILLIDHLATANEK
CCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
TSSIFLPPSKRKARCIFEYVYFARPDSFIFKHSVDKVRRNLGKNLARESTTKQNPDEKEL
CCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVVSVPDSSNTAALGFVRESLKMQRPARFEHGLIRNHYVGRTFIQPGIHSRDIKVRSKYN
EEEECCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHCCCCHHHHCCCCCCCCEEEHHHHH
IVRGVLFDRPIILIDDSIVRGTTARMLIKLIREAEPKSIHLHISSPPITNPCFYGMDFPT
HHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMFDSIDNEEEEIEKIREYIGVDSLKYLSMQGLLNSVPSFEDETCSYCTACFSG
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
DYPIQVTDVTTDKEEND
CCCEEEEEEECCCCCCC
>Mature Secondary Structure
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFRQHKGMGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHHCCCCHHHHH
FKDETLFENLTGYAAIGHNRYSTSGSSKSTNNIQPFSLTYRSGSLAIAHNGNLTNSRALR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
RELTELGVIFQASSDTEIIPHLAARSREKEPVNQIYEALRQVQGAYSMVILANNQLIAAR
HHHHHHCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
DPYGFRPLALGKKTDPLTGELSYIVASETCAFDIIQAEYIRDIEPGEILLIDHLATANEK
CCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
TSSIFLPPSKRKARCIFEYVYFARPDSFIFKHSVDKVRRNLGKNLARESTTKQNPDEKEL
CCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
TVVSVPDSSNTAALGFVRESLKMQRPARFEHGLIRNHYVGRTFIQPGIHSRDIKVRSKYN
EEEECCCCCCCCHHHHHHHHHHHCCCCHHHCCCHHHCCCCHHHHCCCCCCCCEEEHHHHH
IVRGVLFDRPIILIDDSIVRGTTARMLIKLIREAEPKSIHLHISSPPITNPCFYGMDFPT
HHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMFDSIDNEEEEIEKIREYIGVDSLKYLSMQGLLNSVPSFEDETCSYCTACFSG
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
DYPIQVTDVTTDKEEND
CCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]