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The map label for this gene is dfrA [H]

Identifier: 119357727

GI number: 119357727

Start: 2238781

End: 2239830

Strand: Direct

Name: dfrA [H]

Synonym: Cpha266_1931

Alternate gene names: 119357727

Gene position: 2238781-2239830 (Clockwise)

Preceding gene: 119357726

Following gene: 119357734

Centisome position: 71.44

GC content: 51.9

Gene sequence:

>1050_bases
ATGAACATGATTACGCAAAAACCGGTCTGCGTTACGGGCGCATCCGGATTCATTGCCGCTGAGATCATCAAGGCGCTGCT
TTTGAAAGGATACCGGGTAAGAGGAACCGTTCGAAAATCCCCCGATGCTTACCCTTTTCTCGCCTCCCTCGACGGAGCGG
TTGATCGCCTTGAACTTGTCGAGGCTGATCTGTTGAAATCCGGATCGTACAAACAGGCTGTCAAGGGGTGCGAGTATGTA
CTGCATACCGCAAGCCCCTACATCATCAACGTTAAAAACTCGCAACGGGATCTTGTGGAACCTGCCGTCAGGGGAACACA
AGAGGTCATGGAGGCCTGTCTCTTTGCCGGCGGAGTGAAAAGAGTGGTTTTGACCTCCTCCATCGCTGCAGTCACCGACC
AGCCCGACAGTTCCGTTATCTATACCGAAAAAGAGTGGAACAGGCTCTCCTCGCTCAAGCGCAACCCTTACCACTACTCA
AAAACGCTTGCTGAACAGGCGGCATGGAAGTTCATAAAGGAAAAAAATCCGGGTTTTGATCTGATTGCCATCAACCCTGC
CCTGGTGACCGGCCCATCTCTGGCGCCATCGCTCAACACCTCAAACCAGTTGATCCGCGATATCATGATCGGGGTATTCC
CCATGATCATGGATGTAAACTGGGGATTCGTTGACATACGAGACACCGCACGGGCTCACATTCTTGCCATGGAAACTCCA
GCAGCTCAAGGTCGCTACCTCTGCTCAGCCGAAACCATGAACCTCGCAGAGCTGGTTCGCTTTCTGAAAATGGAGGGGTA
TGACGGTTACAGCCTTCCAAAAATAAACCTGTCCGGCAAGGCGGGAACGCTGCTGATGAAGCTGCTATCCTACAGCCAGC
CGAAAGATACGGGCACCTTTATTCGCACTCATGTTGGCCGCCTGTTGCAGATCGATAACAGCAAAATCAGGAAGGATTTC
GGCATGACCTTTATGCCGATAAAAAAGAGCATTCTCGAAGCAGTCAGGGATATGATCGCCTGGGGCCACCTTTCCGGACC
AAAAACGTAA

Upstream 100 bases:

>100_bases
CGGAATTTTCAAGGCACACAAGCAAAGAGCGGAATCCTTTTGACAGGTTTAACGAAATTTTGCATAACATAGCGACCATC
ACTATACGATCTTATTATTC

Downstream 100 bases:

>100_bases
ATTGATTCAGCTTCCTCAAAAAGAGCGAAAACGGCATATCTCTGAATAACTCCCTGTCGCTGATCTTTCTCGATCCCTGT
ATGAGTTTTCGCTTGCTGAG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNMITQKPVCVTGASGFIAAEIIKALLLKGYRVRGTVRKSPDAYPFLASLDGAVDRLELVEADLLKSGSYKQAVKGCEYV
LHTASPYIINVKNSQRDLVEPAVRGTQEVMEACLFAGGVKRVVLTSSIAAVTDQPDSSVIYTEKEWNRLSSLKRNPYHYS
KTLAEQAAWKFIKEKNPGFDLIAINPALVTGPSLAPSLNTSNQLIRDIMIGVFPMIMDVNWGFVDIRDTARAHILAMETP
AAQGRYLCSAETMNLAELVRFLKMEGYDGYSLPKINLSGKAGTLLMKLLSYSQPKDTGTFIRTHVGRLLQIDNSKIRKDF
GMTFMPIKKSILEAVRDMIAWGHLSGPKT

Sequences:

>Translated_349_residues
MNMITQKPVCVTGASGFIAAEIIKALLLKGYRVRGTVRKSPDAYPFLASLDGAVDRLELVEADLLKSGSYKQAVKGCEYV
LHTASPYIINVKNSQRDLVEPAVRGTQEVMEACLFAGGVKRVVLTSSIAAVTDQPDSSVIYTEKEWNRLSSLKRNPYHYS
KTLAEQAAWKFIKEKNPGFDLIAINPALVTGPSLAPSLNTSNQLIRDIMIGVFPMIMDVNWGFVDIRDTARAHILAMETP
AAQGRYLCSAETMNLAELVRFLKMEGYDGYSLPKINLSGKAGTLLMKLLSYSQPKDTGTFIRTHVGRLLQIDNSKIRKDF
GMTFMPIKKSILEAVRDMIAWGHLSGPKT
>Mature_349_residues
MNMITQKPVCVTGASGFIAAEIIKALLLKGYRVRGTVRKSPDAYPFLASLDGAVDRLELVEADLLKSGSYKQAVKGCEYV
LHTASPYIINVKNSQRDLVEPAVRGTQEVMEACLFAGGVKRVVLTSSIAAVTDQPDSSVIYTEKEWNRLSSLKRNPYHYS
KTLAEQAAWKFIKEKNPGFDLIAINPALVTGPSLAPSLNTSNQLIRDIMIGVFPMIMDVNWGFVDIRDTARAHILAMETP
AAQGRYLCSAETMNLAELVRFLKMEGYDGYSLPKINLSGKAGTLLMKLLSYSQPKDTGTFIRTHVGRLLQIDNSKIRKDF
GMTFMPIKKSILEAVRDMIAWGHLSGPKT

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=345, Percent_Identity=35.9420289855072, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321281, Length=360, Percent_Identity=31.3888888888889, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321399, Length=361, Percent_Identity=30.1939058171745, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324421, Length=357, Percent_Identity=30.2521008403361, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320750, Length=262, Percent_Identity=35.4961832061069, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38538; Mature: 38538

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMITQKPVCVTGASGFIAAEIIKALLLKGYRVRGTVRKSPDAYPFLASLDGAVDRLELV
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EADLLKSGSYKQAVKGCEYVLHTASPYIINVKNSQRDLVEPAVRGTQEVMEACLFAGGVK
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCHH
RVVLTSSIAAVTDQPDSSVIYTEKEWNRLSSLKRNPYHYSKTLAEQAAWKFIKEKNPGFD
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LIAINPALVTGPSLAPSLNTSNQLIRDIMIGVFPMIMDVNWGFVDIRDTARAHILAMETP
EEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECC
AAQGRYLCSAETMNLAELVRFLKMEGYDGYSLPKINLSGKAGTLLMKLLSYSQPKDTGTF
CCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHH
IRTHVGRLLQIDNSKIRKDFGMTFMPIKKSILEAVRDMIAWGHLSGPKT
HHHHHHHHEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNMITQKPVCVTGASGFIAAEIIKALLLKGYRVRGTVRKSPDAYPFLASLDGAVDRLELV
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EADLLKSGSYKQAVKGCEYVLHTASPYIINVKNSQRDLVEPAVRGTQEVMEACLFAGGVK
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCHH
RVVLTSSIAAVTDQPDSSVIYTEKEWNRLSSLKRNPYHYSKTLAEQAAWKFIKEKNPGFD
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LIAINPALVTGPSLAPSLNTSNQLIRDIMIGVFPMIMDVNWGFVDIRDTARAHILAMETP
EEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECC
AAQGRYLCSAETMNLAELVRFLKMEGYDGYSLPKINLSGKAGTLLMKLLSYSQPKDTGTF
CCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHH
IRTHVGRLLQIDNSKIRKDFGMTFMPIKKSILEAVRDMIAWGHLSGPKT
HHHHHHHHEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]