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The map label for this gene is grpE [H]

Identifier: 119357669

GI number: 119357669

Start: 2170705

End: 2171328

Strand: Reverse

Name: grpE [H]

Synonym: Cpha266_1873

Alternate gene names: 119357669

Gene position: 2171328-2170705 (Counterclockwise)

Preceding gene: 119357670

Following gene: 119357668

Centisome position: 69.29

GC content: 46.47

Gene sequence:

>624_bases
ATGATTATGAAAAAAGGATCTGCCGATAAAGAAACACCTTTCAATCCTGTTCCTCCTTTTTCCGGAGCGGGTGAATCGGA
ACCCGAACGTTCAGCTCCTTTTGAAGAAGAGCTGGAGTCGTCACAGGAAATGGATCGCTATAAAGCTGAAATAGCTGAAC
TTGAAGCAGAGGTGAGCAGTCAGAAACTGCAGCTCGACAAATATCGCGACGAACTGCTGCGCAGAGCCGCCGAGTTTGAG
AATTTCCGCAAGCAGAAGGAGCGGGAAACCGTTATGGCCGGCTCAAGAGTGCTTGAGAATCTGATCAGGGAGTTTCTTCC
GATGCTTGACGATGTCAAACGGGTGTTGCAGAATCTTCCAGCCGGCGACGAACAGAGTGCGGAAGCGAAACCATATATCG
AGGGTGTTGAACTTTTGAAACGTAATCTCGATTTGTGGCTTGCGGAAAAGGGGGTAAAAGAGATTGAATCGATGGGAAAA
AAACTTGATGTCATGTTTCATGAAGCCATTTCCCTTATAGAGCATCCTGAAGCTGAACCGGATACGATTGTAGATGAGTA
TCAGACAGGCTATCTGCTCGGAGACAAGGTTATCAGGCATGCAAAAGTCATTGTAGCAAAATAA

Upstream 100 bases:

>100_bases
AGAATGGATTACGGTCACGCTGTCAGCGTGCTCAATTATATGGCTGACTGTCTGTCGGCAACACTCTCTGAAGTAAACTA
AACAGTACATATTTGCCATT

Downstream 100 bases:

>100_bases
GTCCGGATCTCTTCCCGATAATCCGGAAACGGATTGGAGGATGAACCGAAACGTAATGTCAAGAGCAATTCTTGTGGTGT
CACTATGAAAAAAGATTACT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MIMKKGSADKETPFNPVPPFSGAGESEPERSAPFEEELESSQEMDRYKAEIAELEAEVSSQKLQLDKYRDELLRRAAEFE
NFRKQKERETVMAGSRVLENLIREFLPMLDDVKRVLQNLPAGDEQSAEAKPYIEGVELLKRNLDLWLAEKGVKEIESMGK
KLDVMFHEAISLIEHPEAEPDTIVDEYQTGYLLGDKVIRHAKVIVAK

Sequences:

>Translated_207_residues
MIMKKGSADKETPFNPVPPFSGAGESEPERSAPFEEELESSQEMDRYKAEIAELEAEVSSQKLQLDKYRDELLRRAAEFE
NFRKQKERETVMAGSRVLENLIREFLPMLDDVKRVLQNLPAGDEQSAEAKPYIEGVELLKRNLDLWLAEKGVKEIESMGK
KLDVMFHEAISLIEHPEAEPDTIVDEYQTGYLLGDKVIRHAKVIVAK
>Mature_207_residues
MIMKKGSADKETPFNPVPPFSGAGESEPERSAPFEEELESSQEMDRYKAEIAELEAEVSSQKLQLDKYRDELLRRAAEFE
NFRKQKERETVMAGSRVLENLIREFLPMLDDVKRVLQNLPAGDEQSAEAKPYIEGVELLKRNLDLWLAEKGVKEIESMGK
KLDVMFHEAISLIEHPEAEPDTIVDEYQTGYLLGDKVIRHAKVIVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=201, Percent_Identity=32.3383084577114, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=174, Percent_Identity=27.0114942528736, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23635; Mature: 23635

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKKGSADKETPFNPVPPFSGAGESEPERSAPFEEELESSQEMDRYKAEIAELEAEVSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLQLDKYRDELLRRAAEFENFRKQKERETVMAGSRVLENLIREFLPMLDDVKRVLQNLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGDEQSAEAKPYIEGVELLKRNLDLWLAEKGVKEIESMGKKLDVMFHEAISLIEHPEAEP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTIVDEYQTGYLLGDKVIRHAKVIVAK
HHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIMKKGSADKETPFNPVPPFSGAGESEPERSAPFEEELESSQEMDRYKAEIAELEAEVSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLQLDKYRDELLRRAAEFENFRKQKERETVMAGSRVLENLIREFLPMLDDVKRVLQNLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGDEQSAEAKPYIEGVELLKRNLDLWLAEKGVKEIESMGKKLDVMFHEAISLIEHPEAEP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTIVDEYQTGYLLGDKVIRHAKVIVAK
HHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA