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The map label for this gene is greA

Identifier: 119357650

GI number: 119357650

Start: 2148198

End: 2148677

Strand: Reverse

Name: greA

Synonym: Cpha266_1854

Alternate gene names: 119357650

Gene position: 2148677-2148198 (Counterclockwise)

Preceding gene: 119357651

Following gene: 119357649

Centisome position: 68.56

GC content: 45.21

Gene sequence:

>480_bases
ATGAATGACAGAATTTACCTGACAAGAGATGGGTACAATCGGCTGAAAGAAGAGCTGTATGTGCTGGTACATCAGAAGAG
AAAGGAAGTTCTGGAAAAAATTGCAGAGGCAAGAGCCCATGGTGACTTGAGTGAAAACGCGGAGTATGACGCAGCAAGAG
AGGAGCAATCCCTGACCGAGGCACATATTGCCGATCTTGAGAACAAACTTTCAACAGCTACTATTCTTGATCCCAAACAG
GTCAAGACCGACAAGGTGTATATCCTGACGTCGGTCAAGCTTCGCGATCTCGATAAAGAGGGAGAGATCATCGAATATAC
GCTTGTATCCTCGGAAGAGGCAGATACCGACCTTGGAAAAATTTCGGTTCGCTCTCCGGTTGGTCGTGCACTCATCGGTA
AAACAGTTGGCGAAAAAGTTCAGATTATGGTGCCTAAAGGAGAGCTGCACTATGAGATATTGGAGATTTTTGTTAAATAG

Upstream 100 bases:

>100_bases
CTTTTTTTATTTGCATAGCCAGTCTGCCGGATTCTTCGGTCGGCATTGGGGCTGCGGCTCGTTTCGGCCATTTATCATCA
CCATAAAAACAGCTAATGAC

Downstream 100 bases:

>100_bases
GCGCAACAGAAACGCCGCGGCTTTTCCTGGATTAACGGGCATAGCATGCTCTTTCCGTTCAGCGATGGCAGGGAGGACAC
AACAAATAACGAACAGCACT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNDRIYLTRDGYNRLKEELYVLVHQKRKEVLEKIAEARAHGDLSENAEYDAAREEQSLTEAHIADLENKLSTATILDPKQ
VKTDKVYILTSVKLRDLDKEGEIIEYTLVSSEEADTDLGKISVRSPVGRALIGKTVGEKVQIMVPKGELHYEILEIFVK

Sequences:

>Translated_159_residues
MNDRIYLTRDGYNRLKEELYVLVHQKRKEVLEKIAEARAHGDLSENAEYDAAREEQSLTEAHIADLENKLSTATILDPKQ
VKTDKVYILTSVKLRDLDKEGEIIEYTLVSSEEADTDLGKISVRSPVGRALIGKTVGEKVQIMVPKGELHYEILEIFVK
>Mature_159_residues
MNDRIYLTRDGYNRLKEELYVLVHQKRKEVLEKIAEARAHGDLSENAEYDAAREEQSLTEAHIADLENKLSTATILDPKQ
VKTDKVYILTSVKLRDLDKEGEIIEYTLVSSEEADTDLGKISVRSPVGRALIGKTVGEKVQIMVPKGELHYEILEIFVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=42.483660130719, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI87082261, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CHLPD (A1BHJ3)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912294.1
- ProteinModelPortal:   A1BHJ3
- SMR:   A1BHJ3
- STRING:   A1BHJ3
- GeneID:   4571196
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1854
- NMPDR:   fig|290317.7.peg.1951
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18084; Mature: 18084

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRIYLTRDGYNRLKEELYVLVHQKRKEVLEKIAEARAHGDLSENAEYDAAREEQSLTE
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AHIADLENKLSTATILDPKQVKTDKVYILTSVKLRDLDKEGEIIEYTLVSSEEADTDLGK
HHHHHHHHHHCEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCE
ISVRSPVGRALIGKTVGEKVQIMVPKGELHYEILEIFVK
EEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MNDRIYLTRDGYNRLKEELYVLVHQKRKEVLEKIAEARAHGDLSENAEYDAAREEQSLTE
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AHIADLENKLSTATILDPKQVKTDKVYILTSVKLRDLDKEGEIIEYTLVSSEEADTDLGK
HHHHHHHHHHCEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCE
ISVRSPVGRALIGKTVGEKVQIMVPKGELHYEILEIFVK
EEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA