Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 119357648

GI number: 119357648

Start: 2147117

End: 2147866

Strand: Reverse

Name: tpiA

Synonym: Cpha266_1852

Alternate gene names: 119357648

Gene position: 2147866-2147117 (Counterclockwise)

Preceding gene: 119357649

Following gene: 119357646

Centisome position: 68.54

GC content: 51.33

Gene sequence:

>750_bases
ATGCGCAAAAAAATTGTTGTTGGTAACTGGAAAATGAATAAAACCGTTACAGAATCCCTCGTACTGGCCGCTGAAGTTAC
AGCCGCACTTGGTGAAGGGTTTACCGGTTGTGACGTGGGTATTGCCCCCCCTTTCACCGCCTTGTATGAAGTTGGCAAAC
TGACCGGGAGCAGGCTCGCACTCGTTGCCCAGAACTGTCATTTTGAGCCGGACGGGGCCTTTACCGGTGAGGTATCGGCA
AGTATGGTGCGTGAGGCCGGTTGTTCTGCGGTGATCGCAGGGCATTCCGAGCGCAGACACTGTTTTGGTGAAACCAACGC
AATTGTCAATCGCAAGGTCAAACATGCTCTTTCCGAAGGATTGCAGGTAATCATGTGTGCAGGAGAAACCCTTGAGCAGC
GTGAAGGCGGAGTTACCGGTGACGTTGTGACCGCACAGGTAAGGGAAGGTCTTCTTGGTATCGACGATATCTCGAATATC
GTGATTGCCTATGAGCCGGTATGGGCTATTGGTACCGGTAAAACTGCGACATCCGAACAGGCAGAAGAGGTGCATCTTTT
GATTCGCAATACCGTCAGCGATCTGTTTGGCGAAGAAGCTGCCGGAAGACTGAGGATTCAGTATGGCGGCAGTGTCAAGC
CCTCAAACGCAAAGGAGCTTTTTTCGATGCCCAATATTGATGGCGGTCTGATTGGTGGAGCAAGTCTTAATGCCGCTGAT
TTTGTCGCAATCGTGAAAGCCGCTGTCTGA

Upstream 100 bases:

>100_bases
GCAGCGTTGAACTGAAGGACGCCCGCGGAGGAAAGCACGCGTTCACTATTGATCAGGTTGATCGAATTTACAGAGATATT
AAAACGGATTACTAAAAATT

Downstream 100 bases:

>100_bases
AACAGAGGCTTCAAATACATATAACATGAAAAGGGTGCTTCAGAAGCACCCTTTTCATGTTATTCGTACCACCTTCAGCA
ACATGATCCCCTCGTTTTTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRKKIVVGNWKMNKTVTESLVLAAEVTAALGEGFTGCDVGIAPPFTALYEVGKLTGSRLALVAQNCHFEPDGAFTGEVSA
SMVREAGCSAVIAGHSERRHCFGETNAIVNRKVKHALSEGLQVIMCAGETLEQREGGVTGDVVTAQVREGLLGIDDISNI
VIAYEPVWAIGTGKTATSEQAEEVHLLIRNTVSDLFGEEAAGRLRIQYGGSVKPSNAKELFSMPNIDGGLIGGASLNAAD
FVAIVKAAV

Sequences:

>Translated_249_residues
MRKKIVVGNWKMNKTVTESLVLAAEVTAALGEGFTGCDVGIAPPFTALYEVGKLTGSRLALVAQNCHFEPDGAFTGEVSA
SMVREAGCSAVIAGHSERRHCFGETNAIVNRKVKHALSEGLQVIMCAGETLEQREGGVTGDVVTAQVREGLLGIDDISNI
VIAYEPVWAIGTGKTATSEQAEEVHLLIRNTVSDLFGEEAAGRLRIQYGGSVKPSNAKELFSMPNIDGGLIGGASLNAAD
FVAIVKAAV
>Mature_249_residues
MRKKIVVGNWKMNKTVTESLVLAAEVTAALGEGFTGCDVGIAPPFTALYEVGKLTGSRLALVAQNCHFEPDGAFTGEVSA
SMVREAGCSAVIAGHSERRHCFGETNAIVNRKVKHALSEGLQVIMCAGETLEQREGGVTGDVVTAQVREGLLGIDDISNI
VIAYEPVWAIGTGKTATSEQAEEVHLLIRNTVSDLFGEEAAGRLRIQYGGSVKPSNAKELFSMPNIDGGLIGGASLNAAD
FVAIVKAAV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=45.5284552845528, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=45.5284552845528, Blast_Score=201, Evalue=5e-52,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=43.4262948207171, Blast_Score=183, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=47.7732793522267, Blast_Score=203, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6320255, Length=253, Percent_Identity=41.897233201581, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=48.3739837398374, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=48.3739837398374, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=48.3739837398374, Blast_Score=214, Evalue=4e-56,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CHLPD (A1BHJ1)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912292.1
- ProteinModelPortal:   A1BHJ1
- SMR:   A1BHJ1
- STRING:   A1BHJ1
- GeneID:   4571194
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1852
- NMPDR:   fig|290317.7.peg.1949
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26115; Mature: 26115

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIVVGNWKMNKTVTESLVLAAEVTAALGEGFTGCDVGIAPPFTALYEVGKLTGSRLA
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
LVAQNCHFEPDGAFTGEVSASMVREAGCSAVIAGHSERRHCFGETNAIVNRKVKHALSEG
EEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHC
LQVIMCAGETLEQREGGVTGDVVTAQVREGLLGIDDISNIVIAYEPVWAIGTGKTATSEQ
CEEEEECCCHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCHHH
AEEVHLLIRNTVSDLFGEEAAGRLRIQYGGSVKPSNAKELFSMPNIDGGLIGGASLNAAD
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCEECCCCCCHHH
FVAIVKAAV
HHHHHHHCC
>Mature Secondary Structure
MRKKIVVGNWKMNKTVTESLVLAAEVTAALGEGFTGCDVGIAPPFTALYEVGKLTGSRLA
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
LVAQNCHFEPDGAFTGEVSASMVREAGCSAVIAGHSERRHCFGETNAIVNRKVKHALSEG
EEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHC
LQVIMCAGETLEQREGGVTGDVVTAQVREGLLGIDDISNIVIAYEPVWAIGTGKTATSEQ
CEEEEECCCHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCHHH
AEEVHLLIRNTVSDLFGEEAAGRLRIQYGGSVKPSNAKELFSMPNIDGGLIGGASLNAAD
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCEECCCCCCHHH
FVAIVKAAV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA