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The map label for this gene is thiC [H]

Identifier: 119357647

GI number: 119357647

Start: 2145318

End: 2147018

Strand: Direct

Name: thiC [H]

Synonym: Cpha266_1851

Alternate gene names: 119357647

Gene position: 2145318-2147018 (Clockwise)

Preceding gene: 119357643

Following gene: 119357653

Centisome position: 68.46

GC content: 50.32

Gene sequence:

>1701_bases
ATGACTCTCATGACTACTTCTTCAGAGACCGGCTTTTGCACCGGAAACCAGACTCCCGGAATCGCTTCAGAAAAAATCTA
TGCCGAAGGCACGATCTTCCCGCTGAAAATCGGCATGAGACAGATAAAGCTCAGCAAAACCTATACAACAAAGGATCGGG
AGTTCTCTTCCTTTCCGCTCTACGATACCAGCGGCCCCTACTCTGATCCCTCGATCGTTACGGATCCAAAAAAAGGACTG
CTTCCGATTCGGGATACGTGGGGATTCAATGACGGAAAAACCTCGCCCTCTAACGACTCCTCGCTTCCCGTAACCATCCC
CGTGCGAAACCCCCTCAAAGCAAAGGAGGGCGTTGGCATCACGCAAATGCACTATGCGCGCAAAGGAGTTATCACTCCGG
AAATGGAGTATGTCGCCATACGGGAGAACCAGTTGCTTGAAAGCCGGGCATCTTCGTTTCACGGCAATCATAACAACGCG
AAACCTGTGACGCCTGAATTTGTCCGGCAGGAGATTGCATGCGGAAGAGCGATCATACCGGCAAACATCAATCATCCCGA
ACTTGAGCCGATGATTATCGGAAAAAACTTCCGGGTAAAAATCAATGCCAATATCGGCAACTCCGCCATGGGATCGTCAA
TTGAACAAGAGGTCGAAAAAGCTGTCTGGGCTTGCCGATGGGGAGCTGACACGATTATGGATCTTAGTACAGGAACCAGC
ATTCACAAAACCCGTGAGTGGATAGTGAGGAACTCTCCGGTCCCTGTGGGAACCGTACCAATATACCAGGCACTCGAAAA
AGTTGGCGGTGTTTCGGAAGCGCTCACCTGGGAGGTTTATCGCGATACCCTGATCGAACAGGCTGAACAGGGCGTTGATT
ACTTCACCATTCACGCAGGAATTCTCCTTGAGCATCTTCCCTATGCTGAAAAGCGCCTTACCGGCATTGTATCACGTGGA
GGCTCAATCATGGCGAAATGGTGTCGGGCACATAAGACGGAAAACTTTCTTTTCACCCATTTTGAAGATATATGCCGCAT
CCTCAAGACCTACGATATCGCCATTTCGCTCGGCGATGCCCTCAGGCCAGGCTCAATCGGCGATGCCAACGATGAGGCCC
AGTTTGGTGAGCTTAAAGTCCTGGGTTCCTTAACGCTCGTCGCATGGGAGCATGATGTCCAGGTAATGATTGAAGGCCCG
GGGCACGTTCCGCTTCATCTGGTGCTTGAAAATATGGAAAAACAGCTTGAACTCTGTCATGAAGCTCCATTTTATACGTT
AGGACCGCTTGTCACCGATATCGCTGCAGGATATGACCACATCAATTCAGCAATCGGCGGCGCTCTGATTGCAAGTTACG
GCTGTTCCATGCTCTGCTACGTTACTCCCAAAGAGCATCTCGGACTTCCTGATAAAAACGATGTTCGCGAAGGGGTTATC
GCTCACAGAGTTGCAGCGCATGCCGCAGACCTTGCAAAAGGAAACCATGCCGCATGGTTGCGCGATGAACTCATGAGCCG
GGCCCGCTACTCTTTTGCCTGGGAAGATCAGTTCAATCTCTCTCTTGATCCTGAAAAAACCCGCGAAGTTTACCGCCAGA
GTATGGCTTCAAGCGTAAACCTGAATAAAAACGCGGATTTTTGCACCATGTGCGGACCGGATTTCTGTTCGATGAAAAAA
TCACGGGAGTTAAACGGGTAA

Upstream 100 bases:

>100_bases
TTGATGCAGGTAATGCTGACGCAAGAAAAGATGAAGCTGGCCTTCCCCTGATTTACGCTTCACTCTCCCCTGCCAGGAGC
ACCTGCTCATTGACTGTTCA

Downstream 100 bases:

>100_bases
AAAACGAGGGGATCATGTTGCTGAAGGTGGTACGAATAACATGAAAAGGGTGCTTCTGAAGCACCCTTTTCATGTTATAT
GTATTTGAAGCCTCTGTTTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTLMTTSSETGFCTGNQTPGIASEKIYAEGTIFPLKIGMRQIKLSKTYTTKDREFSSFPLYDTSGPYSDPSIVTDPKKGL
LPIRDTWGFNDGKTSPSNDSSLPVTIPVRNPLKAKEGVGITQMHYARKGVITPEMEYVAIRENQLLESRASSFHGNHNNA
KPVTPEFVRQEIACGRAIIPANINHPELEPMIIGKNFRVKINANIGNSAMGSSIEQEVEKAVWACRWGADTIMDLSTGTS
IHKTREWIVRNSPVPVGTVPIYQALEKVGGVSEALTWEVYRDTLIEQAEQGVDYFTIHAGILLEHLPYAEKRLTGIVSRG
GSIMAKWCRAHKTENFLFTHFEDICRILKTYDIAISLGDALRPGSIGDANDEAQFGELKVLGSLTLVAWEHDVQVMIEGP
GHVPLHLVLENMEKQLELCHEAPFYTLGPLVTDIAAGYDHINSAIGGALIASYGCSMLCYVTPKEHLGLPDKNDVREGVI
AHRVAAHAADLAKGNHAAWLRDELMSRARYSFAWEDQFNLSLDPEKTREVYRQSMASSVNLNKNADFCTMCGPDFCSMKK
SRELNG

Sequences:

>Translated_566_residues
MTLMTTSSETGFCTGNQTPGIASEKIYAEGTIFPLKIGMRQIKLSKTYTTKDREFSSFPLYDTSGPYSDPSIVTDPKKGL
LPIRDTWGFNDGKTSPSNDSSLPVTIPVRNPLKAKEGVGITQMHYARKGVITPEMEYVAIRENQLLESRASSFHGNHNNA
KPVTPEFVRQEIACGRAIIPANINHPELEPMIIGKNFRVKINANIGNSAMGSSIEQEVEKAVWACRWGADTIMDLSTGTS
IHKTREWIVRNSPVPVGTVPIYQALEKVGGVSEALTWEVYRDTLIEQAEQGVDYFTIHAGILLEHLPYAEKRLTGIVSRG
GSIMAKWCRAHKTENFLFTHFEDICRILKTYDIAISLGDALRPGSIGDANDEAQFGELKVLGSLTLVAWEHDVQVMIEGP
GHVPLHLVLENMEKQLELCHEAPFYTLGPLVTDIAAGYDHINSAIGGALIASYGCSMLCYVTPKEHLGLPDKNDVREGVI
AHRVAAHAADLAKGNHAAWLRDELMSRARYSFAWEDQFNLSLDPEKTREVYRQSMASSVNLNKNADFCTMCGPDFCSMKK
SRELNG
>Mature_565_residues
TLMTTSSETGFCTGNQTPGIASEKIYAEGTIFPLKIGMRQIKLSKTYTTKDREFSSFPLYDTSGPYSDPSIVTDPKKGLL
PIRDTWGFNDGKTSPSNDSSLPVTIPVRNPLKAKEGVGITQMHYARKGVITPEMEYVAIRENQLLESRASSFHGNHNNAK
PVTPEFVRQEIACGRAIIPANINHPELEPMIIGKNFRVKINANIGNSAMGSSIEQEVEKAVWACRWGADTIMDLSTGTSI
HKTREWIVRNSPVPVGTVPIYQALEKVGGVSEALTWEVYRDTLIEQAEQGVDYFTIHAGILLEHLPYAEKRLTGIVSRGG
SIMAKWCRAHKTENFLFTHFEDICRILKTYDIAISLGDALRPGSIGDANDEAQFGELKVLGSLTLVAWEHDVQVMIEGPG
HVPLHLVLENMEKQLELCHEAPFYTLGPLVTDIAAGYDHINSAIGGALIASYGCSMLCYVTPKEHLGLPDKNDVREGVIA
HRVAAHAADLAKGNHAAWLRDELMSRARYSFAWEDQFNLSLDPEKTREVYRQSMASSVNLNKNADFCTMCGPDFCSMKKS
RELNG

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=570, Percent_Identity=58.5964912280702, Blast_Score=687, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 62592; Mature: 62461

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMTTSSETGFCTGNQTPGIASEKIYAEGTIFPLKIGMRQIKLSKTYTTKDREFSSFPL
CEEEECCCCCCEECCCCCCCCCCCEEEECCCEEEEECCCEEEEEECEECCCCCCCCCCCC
YDTSGPYSDPSIVTDPKKGLLPIRDTWGFNDGKTSPSNDSSLPVTIPVRNPLKAKEGVGI
CCCCCCCCCCCEEECCCCCCEECHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCC
TQMHYARKGVITPEMEYVAIRENQLLESRASSFHGNHNNAKPVTPEFVRQEIACGRAIIP
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEE
ANINHPELEPMIIGKNFRVKINANIGNSAMGSSIEQEVEKAVWACRWGADTIMDLSTGTS
CCCCCCCCCEEEECCCEEEEEECCCCCCHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IHKTREWIVRNSPVPVGTVPIYQALEKVGGVSEALTWEVYRDTLIEQAEQGVDYFTIHAG
HHHHHHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
ILLEHLPYAEKRLTGIVSRGGSIMAKWCRAHKTENFLFTHFEDICRILKTYDIAISLGDA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCC
LRPGSIGDANDEAQFGELKVLGSLTLVAWEHDVQVMIEGPGHVPLHLVLENMEKQLELCH
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
EAPFYTLGPLVTDIAAGYDHINSAIGGALIASYGCSMLCYVTPKEHLGLPDKNDVREGVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHH
AHRVAAHAADLAKGNHAAWLRDELMSRARYSFAWEDQFNLSLDPEKTREVYRQSMASSVN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC
LNKNADFCTMCGPDFCSMKKSRELNG
CCCCCCEEEECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TLMTTSSETGFCTGNQTPGIASEKIYAEGTIFPLKIGMRQIKLSKTYTTKDREFSSFPL
EEEECCCCCCEECCCCCCCCCCCEEEECCCEEEEECCCEEEEEECEECCCCCCCCCCCC
YDTSGPYSDPSIVTDPKKGLLPIRDTWGFNDGKTSPSNDSSLPVTIPVRNPLKAKEGVGI
CCCCCCCCCCCEEECCCCCCEECHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCC
TQMHYARKGVITPEMEYVAIRENQLLESRASSFHGNHNNAKPVTPEFVRQEIACGRAIIP
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEE
ANINHPELEPMIIGKNFRVKINANIGNSAMGSSIEQEVEKAVWACRWGADTIMDLSTGTS
CCCCCCCCCEEEECCCEEEEEECCCCCCHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IHKTREWIVRNSPVPVGTVPIYQALEKVGGVSEALTWEVYRDTLIEQAEQGVDYFTIHAG
HHHHHHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
ILLEHLPYAEKRLTGIVSRGGSIMAKWCRAHKTENFLFTHFEDICRILKTYDIAISLGDA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCC
LRPGSIGDANDEAQFGELKVLGSLTLVAWEHDVQVMIEGPGHVPLHLVLENMEKQLELCH
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
EAPFYTLGPLVTDIAAGYDHINSAIGGALIASYGCSMLCYVTPKEHLGLPDKNDVREGVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHH
AHRVAAHAADLAKGNHAAWLRDELMSRARYSFAWEDQFNLSLDPEKTREVYRQSMASSVN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC
LNKNADFCTMCGPDFCSMKKSRELNG
CCCCCCEEEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA