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The map label for this gene is ybjS [C]

Identifier: 119357580

GI number: 119357580

Start: 2030199

End: 2031203

Strand: Reverse

Name: ybjS [C]

Synonym: Cpha266_1783

Alternate gene names: 119357580

Gene position: 2031203-2030199 (Counterclockwise)

Preceding gene: 119357583

Following gene: 119357579

Centisome position: 64.81

GC content: 50.85

Gene sequence:

>1005_bases
ATGACTGTTGCTTTAGTGACCGGCGCATCCGGTTTTATCGGATCTCATCTGGTATCTCGATGTCTCGATGATGGTTTTGA
CGTCAAGGCGCTTGTTCGCAAGGGAAATTCCCGAATCGGACAATTTCGGCGCCAGGGTGTCGATGTTGTTGAGGGTGATC
TTCGAGACCGTGATGCCGTACATCGTGCCGTACATGGGTGCGATTTCGTTTTTCATTCAGCGGCGCTTGCTTCCGATTGG
GGAGATTGGGAGGAGTTCAGGGCGGTTAATATCGAAGGCACGCGTTATATCTGTGAAGCCTCTGAACATGAGGGCGTTCG
GCGACTCGTTTATCTCAGTACCTATGAGGTGTTCGATCATTTTCGGCTTGAGCGTCTTGATGAGCGGGTTCCCTATACAA
AACGCGGGGAACAGTACCCCGATACCAAGATCGAAGGGACAGAGGTTGTGTGGCATTACAAAGAGAGGGGTTTGTCGGTC
AGTGTTGTCTATCCCTCACTTGTTTTTGGTCCCGGAGATAATACCTTTTTTCCTCTTCTTGCGGATGCAATCAGGAAGAG
ACGTTTTTTTTACTGGCAGGGAAGGGCAGGGCTGAACCTGATTTTTATAGATAATCTTGTTGATCTGCTTATGCTGGCGG
CTACCCATCAGGATGCTTCGGGTGAGGATTTTCTTGCCTGTGACGGCAACGGTATAACGCTTGAGGAGCTTTGTATGAGT
ATCGCCAACAGAATCAATGTTCCGGCTCCATCCATCTGCCTGCCGTTTGGTCTGGTGCATTTTCTTGCCCATGTTACAGA
GTTGGTGTACAGCGCAGTCGGTAGCGAAAAAAGGCCGCTGCTGACTCGTCAGGCGGTGAAACTTCTGGCTTCAAAAGTTC
TGGTCGATACCGGTAAAGCTCGCCGCCTGCTTGGCTGGGAGCCCACTGTCCCTCTCGGGAAAGGGGTTGAGCAAACGCTC
GACTGGCTTGTTTCGGTAGATCCGGCTGATTGGAAAAAGAAGTAG

Upstream 100 bases:

>100_bases
TGAATGTTCTTTTTGACTGTTTGATGCGTTACCTTGACGTGTCACTGATTTTGGCCTGATAACTTTTTTGTGTAAAGGAT
AGCTCTAACCGATTTGCATT

Downstream 100 bases:

>100_bases
CACCCGCTATTTTTCTTTTGTCGATTGCTGAAAAAATTCTTACTCTTTGTGGTGTTAGACTGTTCTTTTTCTTTCTGGTG
CCGGATGTAAGGATCCGGAT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTVALVTGASGFIGSHLVSRCLDDGFDVKALVRKGNSRIGQFRRQGVDVVEGDLRDRDAVHRAVHGCDFVFHSAALASDW
GDWEEFRAVNIEGTRYICEASEHEGVRRLVYLSTYEVFDHFRLERLDERVPYTKRGEQYPDTKIEGTEVVWHYKERGLSV
SVVYPSLVFGPGDNTFFPLLADAIRKRRFFYWQGRAGLNLIFIDNLVDLLMLAATHQDASGEDFLACDGNGITLEELCMS
IANRINVPAPSICLPFGLVHFLAHVTELVYSAVGSEKRPLLTRQAVKLLASKVLVDTGKARRLLGWEPTVPLGKGVEQTL
DWLVSVDPADWKKK

Sequences:

>Translated_334_residues
MTVALVTGASGFIGSHLVSRCLDDGFDVKALVRKGNSRIGQFRRQGVDVVEGDLRDRDAVHRAVHGCDFVFHSAALASDW
GDWEEFRAVNIEGTRYICEASEHEGVRRLVYLSTYEVFDHFRLERLDERVPYTKRGEQYPDTKIEGTEVVWHYKERGLSV
SVVYPSLVFGPGDNTFFPLLADAIRKRRFFYWQGRAGLNLIFIDNLVDLLMLAATHQDASGEDFLACDGNGITLEELCMS
IANRINVPAPSICLPFGLVHFLAHVTELVYSAVGSEKRPLLTRQAVKLLASKVLVDTGKARRLLGWEPTVPLGKGVEQTL
DWLVSVDPADWKKK
>Mature_333_residues
TVALVTGASGFIGSHLVSRCLDDGFDVKALVRKGNSRIGQFRRQGVDVVEGDLRDRDAVHRAVHGCDFVFHSAALASDWG
DWEEFRAVNIEGTRYICEASEHEGVRRLVYLSTYEVFDHFRLERLDERVPYTKRGEQYPDTKIEGTEVVWHYKERGLSVS
VVYPSLVFGPGDNTFFPLLADAIRKRRFFYWQGRAGLNLIFIDNLVDLLMLAATHQDASGEDFLACDGNGITLEELCMSI
ANRINVPAPSICLPFGLVHFLAHVTELVYSAVGSEKRPLLTRQAVKLLASKVLVDTGKARRLLGWEPTVPLGKGVEQTLD
WLVSVDPADWKKK

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI239745448, Length=328, Percent_Identity=28.9634146341463, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI193211614, Length=329, Percent_Identity=26.1398176291793, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI8393516, Length=329, Percent_Identity=26.1398176291793, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI310132178, Length=328, Percent_Identity=28.6585365853659, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI310113012, Length=328, Percent_Identity=28.6585365853659, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI116268111, Length=329, Percent_Identity=27.6595744680851, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI87081792, Length=330, Percent_Identity=24.8484848484848, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71987463, Length=193, Percent_Identity=31.6062176165803, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321437, Length=342, Percent_Identity=25.1461988304094, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 37453; Mature: 37322

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVALVTGASGFIGSHLVSRCLDDGFDVKALVRKGNSRIGQFRRQGVDVVEGDLRDRDAV
CEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
HRAVHGCDFVFHSAALASDWGDWEEFRAVNIEGTRYICEASEHEGVRRLVYLSTYEVFDH
HHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
FRLERLDERVPYTKRGEQYPDTKIEGTEVVWHYKERGLSVSVVYPSLVFGPGDNTFFPLL
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHCCCEEEEEECEEEECCCCCCHHHHH
ADAIRKRRFFYWQGRAGLNLIFIDNLVDLLMLAATHQDASGEDFLACDGNGITLEELCMS
HHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
IANRINVPAPSICLPFGLVHFLAHVTELVYSAVGSEKRPLLTRQAVKLLASKVLVDTGKA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
RRLLGWEPTVPLGKGVEQTLDWLVSVDPADWKKK
CEECCCCCCCCCCCCHHHHHHHHEECCCCCCCCC
>Mature Secondary Structure 
TVALVTGASGFIGSHLVSRCLDDGFDVKALVRKGNSRIGQFRRQGVDVVEGDLRDRDAV
EEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
HRAVHGCDFVFHSAALASDWGDWEEFRAVNIEGTRYICEASEHEGVRRLVYLSTYEVFDH
HHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
FRLERLDERVPYTKRGEQYPDTKIEGTEVVWHYKERGLSVSVVYPSLVFGPGDNTFFPLL
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHCCCEEEEEECEEEECCCCCCHHHHH
ADAIRKRRFFYWQGRAGLNLIFIDNLVDLLMLAATHQDASGEDFLACDGNGITLEELCMS
HHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
IANRINVPAPSICLPFGLVHFLAHVTELVYSAVGSEKRPLLTRQAVKLLASKVLVDTGKA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
RRLLGWEPTVPLGKGVEQTLDWLVSVDPADWKKK
CEECCCCCCCCCCCCHHHHHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]