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The map label for this gene is nemA [H]

Identifier: 119357369

GI number: 119357369

Start: 1782996

End: 1784075

Strand: Reverse

Name: nemA [H]

Synonym: Cpha266_1567

Alternate gene names: 119357369

Gene position: 1784075-1782996 (Counterclockwise)

Preceding gene: 119357377

Following gene: 119357368

Centisome position: 56.93

GC content: 52.22

Gene sequence:

>1080_bases
ATGTCGATATTATTTAATCCATTCACTCTCGGCCCGTTACATCTTCGGAACCGTCTGGTAATGGCGCCGATGACCCGGAG
TCGAGCAACCGGCAATATTCCAAACTCGTTGATGACGCGGTATTATGCGGAACGGAGCGCAGCAGGGCTTCTTATTACCG
AGGGGACATCGTCTTCACCCAACGGACTTGGTTATCCGCGTATACCGGGTATATTCTCCGCCGCGCAGGTCGACGGATGG
AAAGCCGTCACCGAAGCGGTCCATGAGAAGGATTCGAAGATGTTCCTGCAGATCATGCATTGCGGACGGATATCCCATCC
TTTGAACCTGCCGCTTGGCGCCCGTATGCTTGCGCCTTCGTTTGTGCCTGCCGAAGGGAAGATTTACACGGATGCTGAAG
GGTTGCAATCCTTTCCCGTGCCGCAGGCTATGAGCCTTGATGATATCACGGCAACGATTGCCGAATATGCTGTGGCTGCA
ACCAATGCCATAGCTGCGGGGTTTGACGGTATCGAGTTGCACGGAGCGAACGGCTATCTGCTCGAACAGTTCATCCGTCC
TAATTCGAATTTGCGTAAAGATGATTACGGAGGTCCCATTGAAAATCGTGCGCGTTTTGTGCTCGAGGTGGTTGATGCGG
TGATCGGGGCCATCGGCAGGGAGAGGGTTGGTATTCGGCTATCTCCGTTTGGCGTATTCAACGATATGCCCCTCTATGAC
AGGATGGAGGAGGAGTATGGCTGGCTGGCTGAAGAGCTCAATTATTTCGGTCTGGCCTATATTCATCTTGTGGATCATTC
CTCAATGGGCGCACCGTTTGTTCCTGATTCCATAAAGGCAGCGTTTCGCAATGTTTTCAAAGGCGCACTGATTCTCTCCG
GCGGTTACGATCTCCTGCGTGCCGAAAGCGATCTTGAAGCCGGAAAATGCGATCTCATTGCTGTTGGCCGGCCGTTTATT
GCCAATCCCGATCTTGTCGAGCGATGGAAAAACGGAGCTCCTCTCAACATCCCCGATATGGATACGTTTTATACTCCAGG
AGAGAAGGGATATATCGACTATTCGGCGTTGCAGGAGTAA

Upstream 100 bases:

>100_bases
TATTTGTGCCGTCATGAACCCTCAAGTTCAAAACGGTGTTCATAAAAAGAACACGTGCAGACACGAACTGTAGTCTCAAC
ATGTTAACAAGGGCAGCGCT

Downstream 100 bases:

>100_bases
GGGGTGCCGCTTGTCAACCTGTCGCGTCGTGCTGTTTCGTAGAACGGTGTTCGAAATGACGGGGCGATACAGGAAAAGTC
CTGTTCTTTTTTGCAGGAGG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSILFNPFTLGPLHLRNRLVMAPMTRSRATGNIPNSLMTRYYAERSAAGLLITEGTSSSPNGLGYPRIPGIFSAAQVDGW
KAVTEAVHEKDSKMFLQIMHCGRISHPLNLPLGARMLAPSFVPAEGKIYTDAEGLQSFPVPQAMSLDDITATIAEYAVAA
TNAIAAGFDGIELHGANGYLLEQFIRPNSNLRKDDYGGPIENRARFVLEVVDAVIGAIGRERVGIRLSPFGVFNDMPLYD
RMEEEYGWLAEELNYFGLAYIHLVDHSSMGAPFVPDSIKAAFRNVFKGALILSGGYDLLRAESDLEAGKCDLIAVGRPFI
ANPDLVERWKNGAPLNIPDMDTFYTPGEKGYIDYSALQE

Sequences:

>Translated_359_residues
MSILFNPFTLGPLHLRNRLVMAPMTRSRATGNIPNSLMTRYYAERSAAGLLITEGTSSSPNGLGYPRIPGIFSAAQVDGW
KAVTEAVHEKDSKMFLQIMHCGRISHPLNLPLGARMLAPSFVPAEGKIYTDAEGLQSFPVPQAMSLDDITATIAEYAVAA
TNAIAAGFDGIELHGANGYLLEQFIRPNSNLRKDDYGGPIENRARFVLEVVDAVIGAIGRERVGIRLSPFGVFNDMPLYD
RMEEEYGWLAEELNYFGLAYIHLVDHSSMGAPFVPDSIKAAFRNVFKGALILSGGYDLLRAESDLEAGKCDLIAVGRPFI
ANPDLVERWKNGAPLNIPDMDTFYTPGEKGYIDYSALQE
>Mature_358_residues
SILFNPFTLGPLHLRNRLVMAPMTRSRATGNIPNSLMTRYYAERSAAGLLITEGTSSSPNGLGYPRIPGIFSAAQVDGWK
AVTEAVHEKDSKMFLQIMHCGRISHPLNLPLGARMLAPSFVPAEGKIYTDAEGLQSFPVPQAMSLDDITATIAEYAVAAT
NAIAAGFDGIELHGANGYLLEQFIRPNSNLRKDDYGGPIENRARFVLEVVDAVIGAIGRERVGIRLSPFGVFNDMPLYDR
MEEEYGWLAEELNYFGLAYIHLVDHSSMGAPFVPDSIKAAFRNVFKGALILSGGYDLLRAESDLEAGKCDLIAVGRPFIA
NPDLVERWKNGAPLNIPDMDTFYTPGEKGYIDYSALQE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=41.1111111111111, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=29.6829971181556, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=202, Percent_Identity=36.6336633663366, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=303, Percent_Identity=29.042904290429, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=204, Percent_Identity=33.8235294117647, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=27.3504273504274, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=258, Percent_Identity=29.4573643410853, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=254, Percent_Identity=29.5275590551181, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=383, Percent_Identity=42.0365535248042, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=40.9448818897638, Blast_Score=230, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39311; Mature: 39180

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILFNPFTLGPLHLRNRLVMAPMTRSRATGNIPNSLMTRYYAERSAAGLLITEGTSSSP
CCEEECCCCCCHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
NGLGYPRIPGIFSAAQVDGWKAVTEAVHEKDSKMFLQIMHCGRISHPLNLPLGARMLAPS
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
FVPAEGKIYTDAEGLQSFPVPQAMSLDDITATIAEYAVAATNAIAAGFDGIELHGANGYL
CCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
LEQFIRPNSNLRKDDYGGPIENRARFVLEVVDAVIGAIGRERVGIRLSPFGVFNDMPLYD
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHH
RMEEEYGWLAEELNYFGLAYIHLVDHSSMGAPFVPDSIKAAFRNVFKGALILSGGYDLLR
HHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHC
AESDLEAGKCDLIAVGRPFIANPDLVERWKNGAPLNIPDMDTFYTPGEKGYIDYSALQE
CCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCEECCCCCCEECHHHHCC
>Mature Secondary Structure 
SILFNPFTLGPLHLRNRLVMAPMTRSRATGNIPNSLMTRYYAERSAAGLLITEGTSSSP
CEEECCCCCCHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
NGLGYPRIPGIFSAAQVDGWKAVTEAVHEKDSKMFLQIMHCGRISHPLNLPLGARMLAPS
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
FVPAEGKIYTDAEGLQSFPVPQAMSLDDITATIAEYAVAATNAIAAGFDGIELHGANGYL
CCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
LEQFIRPNSNLRKDDYGGPIENRARFVLEVVDAVIGAIGRERVGIRLSPFGVFNDMPLYD
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHH
RMEEEYGWLAEELNYFGLAYIHLVDHSSMGAPFVPDSIKAAFRNVFKGALILSGGYDLLR
HHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHC
AESDLEAGKCDLIAVGRPFIANPDLVERWKNGAPLNIPDMDTFYTPGEKGYIDYSALQE
CCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCEECCCCCCEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]