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The map label for this gene is ogt [H]

Identifier: 119357367

GI number: 119357367

Start: 1779677

End: 1780180

Strand: Reverse

Name: ogt [H]

Synonym: Cpha266_1565

Alternate gene names: 119357367

Gene position: 1780180-1779677 (Counterclockwise)

Preceding gene: 119357368

Following gene: 119357366

Centisome position: 56.8

GC content: 48.41

Gene sequence:

>504_bases
ATGAAGCTGTTTTGTAAAAAAACAGGGATTGGTACGATTGGCATTGTGGATTCGGGAGGTGTTGTATCCAATCTTTTTTT
CGATCCTTGCTCTGTTTCACAGGAGATCGAGCGAGGAGAAACCCCGCTTGCCATCGAGGCTTTTCGTCAGCTTGACGCCT
ATCTGAGGGGCGATTTGAAGGCTTTTTCGTTACCTCTTGAACCGACAGGAACTCCATTCATGAAGCGTGTATGGGAAGTT
CTTTCGGTCATTCCCTATGCAACCAGAGTGACTTATAGTGAAATTGCCAAAGCGGCAGGGAATCCTCGCGCCGTGAGGTC
GGTTGCGAGCGCGTGCGGCAGAAACCCCTTGCCTTTGTTTATTCCCTGTCACCGGGTCGTGCGCAGCGACGGCAAGATGG
GCGGTTATCTGGGTGGAAGTGTTTTGAAAGCAGGTTTGCTTGAATTAGAAAAAAAATCAGCATATGATGACCCCTCCTTT
TATATGAACGTTCACGATTGTTGA

Upstream 100 bases:

>100_bases
TTTGTTGGTACCTGTAAGTTTTTATGATCTGATGGGTCGGGACGCGCGATTATTTATTGATAGGTAAAAGTCTGATTGGT
AAGGCAAGGCGGGGGAGTGT

Downstream 100 bases:

>100_bases
TATTCCGGGGGGCAGATTTTGTTTTTATTGAGCTTTAAAAGATTACGGGTAATGGTTATTAATAAAACAAACCGCATTGT
CATGTTACGCAAACACAGGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MKLFCKKTGIGTIGIVDSGGVVSNLFFDPCSVSQEIERGETPLAIEAFRQLDAYLRGDLKAFSLPLEPTGTPFMKRVWEV
LSVIPYATRVTYSEIAKAAGNPRAVRSVASACGRNPLPLFIPCHRVVRSDGKMGGYLGGSVLKAGLLELEKKSAYDDPSF
YMNVHDC

Sequences:

>Translated_167_residues
MKLFCKKTGIGTIGIVDSGGVVSNLFFDPCSVSQEIERGETPLAIEAFRQLDAYLRGDLKAFSLPLEPTGTPFMKRVWEV
LSVIPYATRVTYSEIAKAAGNPRAVRSVASACGRNPLPLFIPCHRVVRSDGKMGGYLGGSVLKAGLLELEKKSAYDDPSF
YMNVHDC
>Mature_167_residues
MKLFCKKTGIGTIGIVDSGGVVSNLFFDPCSVSQEIERGETPLAIEAFRQLDAYLRGDLKAFSLPLEPTGTPFMKRVWEV
LSVIPYATRVTYSEIAKAAGNPRAVRSVASACGRNPLPLFIPCHRVVRSDGKMGGYLGGSVLKAGLLELEKKSAYDDPSF
YMNVHDC

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=103, Percent_Identity=36.8932038834951, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1788542, Length=108, Percent_Identity=44.4444444444444, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=40.1960784313725, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=106, Percent_Identity=37.7358490566038, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=106, Percent_Identity=37.7358490566038, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=45.8823529411765, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18163; Mature: 18163

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFCKKTGIGTIGIVDSGGVVSNLFFDPCSVSQEIERGETPLAIEAFRQLDAYLRGDLK
CCEEECCCCCCEEEEECCCCHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
AFSLPLEPTGTPFMKRVWEVLSVIPYATRVTYSEIAKAAGNPRAVRSVASACGRNPLPLF
HEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
IPCHRVVRSDGKMGGYLGGSVLKAGLLELEKKSAYDDPSFYMNVHDC
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure
MKLFCKKTGIGTIGIVDSGGVVSNLFFDPCSVSQEIERGETPLAIEAFRQLDAYLRGDLK
CCEEECCCCCCEEEEECCCCHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
AFSLPLEPTGTPFMKRVWEVLSVIPYATRVTYSEIAKAAGNPRAVRSVASACGRNPLPLF
HEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
IPCHRVVRSDGKMGGYLGGSVLKAGLLELEKKSAYDDPSFYMNVHDC
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]