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The map label for this gene is hsdS [H]

Identifier: 119357207

GI number: 119357207

Start: 1587158

End: 1588831

Strand: Reverse

Name: hsdS [H]

Synonym: Cpha266_1398

Alternate gene names: 119357207

Gene position: 1588831-1587158 (Counterclockwise)

Preceding gene: 119357208

Following gene: 119357206

Centisome position: 50.7

GC content: 49.16

Gene sequence:

>1674_bases
ATGAATAAACAGATCGCCAACCTGCTTGAGCAGCACTTCGATACAGCCTTTGCCGCCCCTGATGGCATCAAAAAGCTCCG
TGAGCTGATTCTCACCCTCGCCATGCAGGGCAAGCTGGTGGAGCAGGACCCGAACGACCAGCCCGCCTCAGAACTGCTGA
AAGAGATCGAAGCCGAGAAACAGCGGCTGGTGACGGCAGGAAAAATCAAAAAGCCTAAACCTCTGTCTGCAATCGTCAAT
GATGAAATACCATACGATATTCCTTCAAGTTGGATATGGGTGCGATTCGGCGATATTGCCCGGCACAATTCTGGTAAGAC
GCTGGACAAGGGGCGTAACACAGGTGAGTCCCGTGATTACATAACCACGTCAAATCTCTACTGGGGAAAGTTCGAACTCG
AAAATGTTCGTCAAATGTTAATCAGAGAGGATGAACTCGAAAAATGCACAGCCAAAAAGGACGATCTGTTGATTTGTGAA
GGAGGGGAAGCGGGACGAGCAGCAATGTGGCCCTTCGATTCAGAAGTGTGTTTTCAGAATCATATTCATCGTGCTCGATT
TTACAAAGACATTGACCCGTACTTTGTCTACAGATTTTTCGAGAAATTGAGTGCCACTGGTGAGATCAATCAGCATCGCA
AAGGGGTTGGTATTTCCAATATGTCGAGTAAGTCTTTGGCCTCGATAGTGTTTCCATTGCCCCCCTTTTCCGAACAACAC
CGCATCGTCGCTCGCATCGACCAGTTGATGGCCCGTTGCAATGAGCTGGAAAAGCTGCGCAAGGAACGGGAAGAGAAGCG
GCTGATTGTCCACGCCGCCGCCATCAAGCAACTGTTCGATGCACCGGACGGTTCAGCTTGGGGTTTCATCCAGCAACATT
TCAATGAACTCTACAGTGTCAAAGAAAACGTCGCCGAACTACGCAAAGCCATCCTCCAACTCGCCGTCATGGGTCGCCTC
GTTCCCCAGGATCAGAATGATCCCCCAGCATCGGAGTTGTTGAAGGAGATCGAAAAAGAGAAGGCATCGCACGAATGCAC
GAAGTCACGAAGGAAAGGGGAGAAGCTACCGGAAATTTTTAATGAGGAAATGCCCCACAAAATCCCGTCAAATTGGGCTT
GGGTGCGATTTGGCGACATTGCTCAACACAATTCCGGAAAAACGCTGGACAAGGGGCGCAACACAGGTCAACCACGCGAA
TATATCACTACCTCAAATCTCTACAGGGGAAGGTTCGAGCTTGAAAATGTTCGTCAAATGTTAATCAGAGAGGATGAACT
CGAAAAATGCACAGCCAAAAAGGACGATCTGTTGATTTGTGAAGGAGGGGAAGCGGGGCGAGCAGCAGTGTGGCCCTTCG
ATTCAGAAGTGTGTTTTCAGAATCATATTCATCGTGCTCGATTTTACAAAGACATTGACCCGTACTTTGCCTACAGATTT
TTCGAGAAATTGAGTGCCACTGGTGAGATCAATCAGCATCGCAAAGGAGTAGGTATTTCCAATATGTCGAGTAAGGCTTT
GGCCTCGATTGTGTTTCCATTGCCTCCTCAGCCCGAACAACATCGCATTGTAGCCCGAACCGACCAGTTGATGACGCTGT
GCGACCAACTCGACCAGCAGATCGATGACGCCGTTGGCAAACAGACCGAAATCCTGAATGCTGTGTTGGCGTAG

Upstream 100 bases:

>100_bases
AAATTGTTGGAACCTACGCTTCCGGATTCAGCTCGCAGCAAAAACCAGAAATACCGCCTTACCGATAAGGGCCGCGAAAT
CCTGCGGCAGGAGGGGAGCG

Downstream 100 bases:

>100_bases
GTATAAGGATATCTCAAAAAGAATTCATGATGTTGAATGATACTTGTTTTTGTTGTAACTGTTTAGTAAATAAAAAGATG
CTTATGCGTATCCCTTGATG

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNKQIANLLEQHFDTAFAAPDGIKKLRELILTLAMQGKLVEQDPNDQPASELLKEIEAEKQRLVTAGKIKKPKPLSAIVN
DEIPYDIPSSWIWVRFGDIARHNSGKTLDKGRNTGESRDYITTSNLYWGKFELENVRQMLIREDELEKCTAKKDDLLICE
GGEAGRAAMWPFDSEVCFQNHIHRARFYKDIDPYFVYRFFEKLSATGEINQHRKGVGISNMSSKSLASIVFPLPPFSEQH
RIVARIDQLMARCNELEKLRKEREEKRLIVHAAAIKQLFDAPDGSAWGFIQQHFNELYSVKENVAELRKAILQLAVMGRL
VPQDQNDPPASELLKEIEKEKASHECTKSRRKGEKLPEIFNEEMPHKIPSNWAWVRFGDIAQHNSGKTLDKGRNTGQPRE
YITTSNLYRGRFELENVRQMLIREDELEKCTAKKDDLLICEGGEAGRAAVWPFDSEVCFQNHIHRARFYKDIDPYFAYRF
FEKLSATGEINQHRKGVGISNMSSKALASIVFPLPPQPEQHRIVARTDQLMTLCDQLDQQIDDAVGKQTEILNAVLA

Sequences:

>Translated_557_residues
MNKQIANLLEQHFDTAFAAPDGIKKLRELILTLAMQGKLVEQDPNDQPASELLKEIEAEKQRLVTAGKIKKPKPLSAIVN
DEIPYDIPSSWIWVRFGDIARHNSGKTLDKGRNTGESRDYITTSNLYWGKFELENVRQMLIREDELEKCTAKKDDLLICE
GGEAGRAAMWPFDSEVCFQNHIHRARFYKDIDPYFVYRFFEKLSATGEINQHRKGVGISNMSSKSLASIVFPLPPFSEQH
RIVARIDQLMARCNELEKLRKEREEKRLIVHAAAIKQLFDAPDGSAWGFIQQHFNELYSVKENVAELRKAILQLAVMGRL
VPQDQNDPPASELLKEIEKEKASHECTKSRRKGEKLPEIFNEEMPHKIPSNWAWVRFGDIAQHNSGKTLDKGRNTGQPRE
YITTSNLYRGRFELENVRQMLIREDELEKCTAKKDDLLICEGGEAGRAAVWPFDSEVCFQNHIHRARFYKDIDPYFAYRF
FEKLSATGEINQHRKGVGISNMSSKALASIVFPLPPQPEQHRIVARTDQLMTLCDQLDQQIDDAVGKQTEILNAVLA
>Mature_557_residues
MNKQIANLLEQHFDTAFAAPDGIKKLRELILTLAMQGKLVEQDPNDQPASELLKEIEAEKQRLVTAGKIKKPKPLSAIVN
DEIPYDIPSSWIWVRFGDIARHNSGKTLDKGRNTGESRDYITTSNLYWGKFELENVRQMLIREDELEKCTAKKDDLLICE
GGEAGRAAMWPFDSEVCFQNHIHRARFYKDIDPYFVYRFFEKLSATGEINQHRKGVGISNMSSKSLASIVFPLPPFSEQH
RIVARIDQLMARCNELEKLRKEREEKRLIVHAAAIKQLFDAPDGSAWGFIQQHFNELYSVKENVAELRKAILQLAVMGRL
VPQDQNDPPASELLKEIEKEKASHECTKSRRKGEKLPEIFNEEMPHKIPSNWAWVRFGDIAQHNSGKTLDKGRNTGQPRE
YITTSNLYRGRFELENVRQMLIREDELEKCTAKKDDLLICEGGEAGRAAVWPFDSEVCFQNHIHRARFYKDIDPYFAYRF
FEKLSATGEINQHRKGVGISNMSSKALASIVFPLPPQPEQHRIVARTDQLMTLCDQLDQQIDDAVGKQTEILNAVLA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 63834; Mature: 63834

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQIANLLEQHFDTAFAAPDGIKKLRELILTLAMQGKLVEQDPNDQPASELLKEIEAEK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHH
QRLVTAGKIKKPKPLSAIVNDEIPYDIPSSWIWVRFGDIARHNSGKTLDKGRNTGESRDY
HHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCE
ITTSNLYWGKFELENVRQMLIREDELEKCTAKKDDLLICEGGEAGRAAMWPFDSEVCFQN
EEECCEEECCEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEECCCCCHHHHHH
HIHRARFYKDIDPYFVYRFFEKLSATGEINQHRKGVGISNMSSKSLASIVFPLPPFSEQH
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHEECCCCCCHHH
RIVARIDQLMARCNELEKLRKEREEKRLIVHAAAIKQLFDAPDGSAWGFIQQHFNELYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KENVAELRKAILQLAVMGRLVPQDQNDPPASELLKEIEKEKASHECTKSRRKGEKLPEIF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEEMPHKIPSNWAWVRFGDIAQHNSGKTLDKGRNTGQPREYITTSNLYRGRFELENVRQM
HHHCCCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHEECCCHHCCHHHHHHHHHH
LIREDELEKCTAKKDDLLICEGGEAGRAAVWPFDSEVCFQNHIHRARFYKDIDPYFAYRF
HHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FEKLSATGEINQHRKGVGISNMSSKALASIVFPLPPQPEQHRIVARTDQLMTLCDQLDQQ
HHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDDAVGKQTEILNAVLA
HHHHHCHHHHHHHHHHC
>Mature Secondary Structure
MNKQIANLLEQHFDTAFAAPDGIKKLRELILTLAMQGKLVEQDPNDQPASELLKEIEAEK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHH
QRLVTAGKIKKPKPLSAIVNDEIPYDIPSSWIWVRFGDIARHNSGKTLDKGRNTGESRDY
HHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCE
ITTSNLYWGKFELENVRQMLIREDELEKCTAKKDDLLICEGGEAGRAAMWPFDSEVCFQN
EEECCEEECCEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEECCCCCHHHHHH
HIHRARFYKDIDPYFVYRFFEKLSATGEINQHRKGVGISNMSSKSLASIVFPLPPFSEQH
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHEECCCCCCHHH
RIVARIDQLMARCNELEKLRKEREEKRLIVHAAAIKQLFDAPDGSAWGFIQQHFNELYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KENVAELRKAILQLAVMGRLVPQDQNDPPASELLKEIEKEKASHECTKSRRKGEKLPEIF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEEMPHKIPSNWAWVRFGDIAQHNSGKTLDKGRNTGQPREYITTSNLYRGRFELENVRQM
HHHCCCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHEECCCHHCCHHHHHHHHHH
LIREDELEKCTAKKDDLLICEGGEAGRAAVWPFDSEVCFQNHIHRARFYKDIDPYFAYRF
HHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FEKLSATGEINQHRKGVGISNMSSKALASIVFPLPPQPEQHRIVARTDQLMTLCDQLDQQ
HHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDDAVGKQTEILNAVLA
HHHHHCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]