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The map label for this gene is trmH [H]

Identifier: 119357014

GI number: 119357014

Start: 1358714

End: 1359388

Strand: Reverse

Name: trmH [H]

Synonym: Cpha266_1198

Alternate gene names: 119357014

Gene position: 1359388-1358714 (Counterclockwise)

Preceding gene: 119357019

Following gene: 119357009

Centisome position: 43.38

GC content: 52.44

Gene sequence:

>675_bases
ATGCTTGCGCATCGGCAGCCTGATCTTTCGCTGGTAATGGACAATGTCAATAAGGCACATAACCTCTCCGCCATAATAAG
AAGCTGTGATGCCGTGGGCATCCATGAAATTCATGCGGTATCATATCGCAAAAACATTTTTGATCGCCAAACCGCAGCCG
CGGGAACAACCCGATGGGTTGAGGTTACGCTGCATCAATCGATTACAAGCGGCCTCTCCATGATTGCGGGGCGGGGCATG
CAGATTCTTGCCGCATCAGGAAGCAGCAAGAACGTGGATTTCAGAAGCATTGATTACACCCGGCCAACAGCGTTGATTCT
CGGGGCGGAATGGGATGGCGTTTCAACCGAAGCGCTTGAGGCTGCCGACCATCACATATCGATACCCATGCATGGCATGA
TCGAGTCGCTCAATGTATCGGTAGCGGCGGCAGTCATTCTGTTTGAAGCCGAGCGTCAACGAACTGCCGCAGGAATGTAC
AGCCGGCCAATGCTTGAAAAAGAGTCATTTGACCGGCTTCTTTTTGAAAACGCCTACCCCCGGCTTGGCAGGATACTCCG
GGAAAAAGGATTACCCTATCCGGAACTGGATGTTAACGGGCAACTCCCCTCCTCCTGCCACGACTCTGACATGCCCGACA
CGGAAGGAGTGTGTTCAGATTCCGGGCTTTATTAA

Upstream 100 bases:

>100_bases
GAAAAAATATCTTTCTTGTACGACAAATCATCTTCTTTTTCTGAAATCATTCGCAAAAACAACTTTGATCGCTCCTGAAC
GATTCTATAAAATACGCAAA

Downstream 100 bases:

>100_bases
CGAATTGATTCAAGGAATCGCTCGGCATCGACAGCGGCCATACAGCCGGTACCCGCTGCCGTTATGGCTTGACGATAGGT
GTAATCCTGCACGTCACCAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLAHRQPDLSLVMDNVNKAHNLSAIIRSCDAVGIHEIHAVSYRKNIFDRQTAAAGTTRWVEVTLHQSITSGLSMIAGRGM
QILAASGSSKNVDFRSIDYTRPTALILGAEWDGVSTEALEAADHHISIPMHGMIESLNVSVAAAVILFEAERQRTAAGMY
SRPMLEKESFDRLLFENAYPRLGRILREKGLPYPELDVNGQLPSSCHDSDMPDTEGVCSDSGLY

Sequences:

>Translated_224_residues
MLAHRQPDLSLVMDNVNKAHNLSAIIRSCDAVGIHEIHAVSYRKNIFDRQTAAAGTTRWVEVTLHQSITSGLSMIAGRGM
QILAASGSSKNVDFRSIDYTRPTALILGAEWDGVSTEALEAADHHISIPMHGMIESLNVSVAAAVILFEAERQRTAAGMY
SRPMLEKESFDRLLFENAYPRLGRILREKGLPYPELDVNGQLPSSCHDSDMPDTEGVCSDSGLY
>Mature_224_residues
MLAHRQPDLSLVMDNVNKAHNLSAIIRSCDAVGIHEIHAVSYRKNIFDRQTAAAGTTRWVEVTLHQSITSGLSMIAGRGM
QILAASGSSKNVDFRSIDYTRPTALILGAEWDGVSTEALEAADHHISIPMHGMIESLNVSVAAAVILFEAERQRTAAGMY
SRPMLEKESFDRLLFENAYPRLGRILREKGLPYPELDVNGQLPSSCHDSDMPDTEGVCSDSGLY

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=204, Percent_Identity=51.9607843137255, Blast_Score=216, Evalue=7e-58,
Organism=Escherichia coli, GI1790623, Length=152, Percent_Identity=34.8684210526316, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 24568; Mature: 24568

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAHRQPDLSLVMDNVNKAHNLSAIIRSCDAVGIHEIHAVSYRKNIFDRQTAAAGTTRWV
CCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
EVTLHQSITSGLSMIAGRGMQILAASGSSKNVDFRSIDYTRPTALILGAEWDGVSTEALE
EEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHH
AADHHISIPMHGMIESLNVSVAAAVILFEAERQRTAAGMYSRPMLEKESFDRLLFENAYP
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHCCCH
RLGRILREKGLPYPELDVNGQLPSSCHDSDMPDTEGVCSDSGLY
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLAHRQPDLSLVMDNVNKAHNLSAIIRSCDAVGIHEIHAVSYRKNIFDRQTAAAGTTRWV
CCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
EVTLHQSITSGLSMIAGRGMQILAASGSSKNVDFRSIDYTRPTALILGAEWDGVSTEALE
EEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHH
AADHHISIPMHGMIESLNVSVAAAVILFEAERQRTAAGMYSRPMLEKESFDRLLFENAYP
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHCCCH
RLGRILREKGLPYPELDVNGQLPSSCHDSDMPDTEGVCSDSGLY
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]