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The map label for this gene is nuoB [H]

Identifier: 119356790

GI number: 119356790

Start: 1103162

End: 1103731

Strand: Direct

Name: nuoB [H]

Synonym: Cpha266_0962

Alternate gene names: 119356790

Gene position: 1103162-1103731 (Clockwise)

Preceding gene: 119356789

Following gene: 119356791

Centisome position: 35.2

GC content: 50.35

Gene sequence:

>570_bases
ATGGGTTTACTTGATGCCGGAATAACAAAGCATAATGTGCTGATAACTTCGGTTGACAATGTCCTCAACTGGGCGAGATT
GTCTTCACTCTGGCCTATGGGTTTCGGGCTTGCCTGTTGCGCCATTGAGATGATGGCAACCAACGCTTCTAACTACGATC
TCGAGCGGTTCGGAATTTTTCCTCGCTCATCGCCTCGTCAGTCTGATCTCATGCTTGTTGCAGGCACGGTTTCCATGAAA
ATGGCCGAGCGGGTTGTGCGCCTCTACGAGCAGATGCCTGAGCCGCGCTATGTGCTTTCGATGGGCAGTTGCTCAAACTG
CGGGGGGCCTTACTGGGAGCATGGATACCATGTGCTCAAGGGTGTTGACCGTATTATTCCCGTTGATGTTTATGTTCCGG
GTTGTCCTCCAAGACCTGAATCGCTGATTGGTGGTTTGATGAAGGTGCAGGAACTTATCAGAATGGAGCAGATCGGTATT
TCAAGGGCCGATGCGCTTAAAAAACTCGCTGAAAAAAGCGTTGACCCCAGAGTTGTTATTGAAGAGGAACGCAAGGCGTT
GAGGGCATAA

Upstream 100 bases:

>100_bases
GGTAAGGCCTGAACCGAAAGTTCCCCAAATGCCTGTCATGCCTGACCGAAAGGCTGAAGGCGGCAGGGCATAACTCTATA
ATTTCGATTTTTACAGCGAT

Downstream 100 bases:

>100_bases
CAATAAAGCTTAATACCCGGTATATACATGGAACCAGGTTTGGAAATGACACAGCAGGTAAAGCAGGCGCTCGCAGCGTA
CGACGTTGTCCGCGAGAAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MGLLDAGITKHNVLITSVDNVLNWARLSSLWPMGFGLACCAIEMMATNASNYDLERFGIFPRSSPRQSDLMLVAGTVSMK
MAERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLKGVDRIIPVDVYVPGCPPRPESLIGGLMKVQELIRMEQIGI
SRADALKKLAEKSVDPRVVIEEERKALRA

Sequences:

>Translated_189_residues
MGLLDAGITKHNVLITSVDNVLNWARLSSLWPMGFGLACCAIEMMATNASNYDLERFGIFPRSSPRQSDLMLVAGTVSMK
MAERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLKGVDRIIPVDVYVPGCPPRPESLIGGLMKVQELIRMEQIGI
SRADALKKLAEKSVDPRVVIEEERKALRA
>Mature_188_residues
GLLDAGITKHNVLITSVDNVLNWARLSSLWPMGFGLACCAIEMMATNASNYDLERFGIFPRSSPRQSDLMLVAGTVSMKM
AERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLKGVDRIIPVDVYVPGCPPRPESLIGGLMKVQELIRMEQIGIS
RADALKKLAEKSVDPRVVIEEERKALRA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=54.3624161073826, Blast_Score=189, Evalue=9e-49,
Organism=Escherichia coli, GI1788624, Length=153, Percent_Identity=49.6732026143791, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1789074, Length=111, Percent_Identity=41.4414414414414, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788834, Length=156, Percent_Identity=33.974358974359, Blast_Score=91, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=149, Percent_Identity=51.6778523489933, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=52.8571428571429, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=52.8571428571429, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=54.014598540146, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21025; Mature: 20894

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLDAGITKHNVLITSVDNVLNWARLSSLWPMGFGLACCAIEMMATNASNYDLERFGIF
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCC
PRSSPRQSDLMLVAGTVSMKMAERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLK
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHH
GVDRIIPVDVYVPGCPPRPESLIGGLMKVQELIRMEQIGISRADALKKLAEKSVDPRVVI
HHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
EEERKALRA
HHHHHHHCC
>Mature Secondary Structure 
GLLDAGITKHNVLITSVDNVLNWARLSSLWPMGFGLACCAIEMMATNASNYDLERFGIF
CCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCC
PRSSPRQSDLMLVAGTVSMKMAERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLK
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHH
GVDRIIPVDVYVPGCPPRPESLIGGLMKVQELIRMEQIGISRADALKKLAEKSVDPRVVI
HHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
EEERKALRA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA