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The map label for this gene is ilvB [H]

Identifier: 119356684

GI number: 119356684

Start: 975704

End: 977425

Strand: Reverse

Name: ilvB [H]

Synonym: Cpha266_0853

Alternate gene names: 119356684

Gene position: 977425-975704 (Counterclockwise)

Preceding gene: 119356685

Following gene: 119356683

Centisome position: 31.19

GC content: 50.41

Gene sequence:

>1722_bases
ATGAATGTACCAGGTCAAACACTGAACGGTTCAGAAATATTTTTTGAATGTCTGAGAAGAGAAAACGTTGAATATATCTT
CGGGTATCCCGGAGGAGCCCTGCTCAAGGTTTATGAAACGCTCTACGATATCAAGGATATCAAGCACATCCTTGTCCGCC
ATGAACAGGGTGCGGCACACATGGCTGAAGGCTATGCCCGCGCCACCGGAAAACCCGGTATCGTGCTGGTAACGTCAGGA
CCGGGAGCAACCAATACCGTTACGGGCATTGCCAATGCCTACATGGACTCTTCGCCAATGGTGGTTTTTACCGGTCAGGT
ACCCAGTTCGCTTATCGGCAATGACGCATTCCAGGAAGCTGATATCGTGGGCATCACCCGTCCGATCACCAAACACAACT
TCCTTGTCAGGGATGTCAGAGAACTTGCGTTAACAATCCGCAAGGCCTTTTACCTTGCTACAAACGGCCGTCCGGGACCG
GTTCTTGTCGATATGCCCAAAGACATACTGAACGCTTCATGCGAATTTCAGTGGCCCGAAAACATAGAGATCCGGGGTTT
CAAGCCGACAGTAAAATGTCACATGAACCAGGTTGAAAAAGCCGCTCTGAAAATATCGAAAGCTAAAAAACCGCTGTTCT
ATATCGGTGGCGGCGTTATCAGTGCCAATGCATCGGAAGAGCTCCGCAAACTTGCCATTCAGCAGAACATTCCTGTCACC
ATGACCCTTCAGGGCCTTGGCGCCTTTCCGGGAGACCATCCGCTCAGCATGGGAATGCTCGGCATGCATGGCACCTACTG
GGCCAACCAGGCCGTTAACAACTGCGATCTGTTGATTGCTGTCGGAGCACGCTTTGACGACAGGGTTACCGGTAAAGTCG
AAACATTCGCCACACAGGCATACAAGATTCATAATGACATAGACCCGACCAACGTCGATAAAAACATAAAAGTCGATCTT
CCGATTGTCGGTGATGCGAAGCATTTTCTTTCCGCTCTTCTTGACGAGATGCCCGAAGCGACCGAAAACAGGGAGCCCTG
GCTTCAGGAAATCCATAAATGGCAAGAGCAGTGTCCGCTTGCCTATGAAACCGGTGATGAGTTACTGAGAACCGAGTTTG
TCATTGAAGAGACATCAAAACAAACCAAAGGAAATGCGGTCGTCGTAACCGACGTCGGGCAGCATCAGATGTGGACATCC
CAGTACTACAAGTTCAATAACCCCCGTTCCATCATCACCAGCGGAGGACTCGGAACCATGGGCTATGGCCTGCCGGCGTC
TATCGGCGCAGCATTCGGCGTCACAGACCGCCCGATAATCCTGTTCTGCGGAGATGGCGGATTTATGATGAACGTACAGG
AACTCGTCACCGCCGTCCACTATAAAATACCGGTGAAAATTTTCCTCATCAACAACACATTCCTCGGTATGGTTCGACAG
TGGCAGGAACTTTTTCACAAGGAACAGTACTCCTTTACCGATCTTGGTAACAGCAATCCCGACTTCGTCAAGGTTGCCGA
TGCCTTCGGCTGCAGAACGTCCTGCGCAGAAACGCCGGATCAGGCCCGTCAGGCGATAAAGGAGGCTCTTGCATGGAACG
AAGGACCGTTCCTTGTCGAATTCAAGGTTATCAAAAAAGATATGGTATTTCCCATGGTCCCTGCGGGCGGCTCTATTTCA
GACATGATGCTTGCCCGGTTAAACCCAAAAACAATGGTTTGA

Upstream 100 bases:

>100_bases
AAAAAAGGGTATCTGGCAAGATATGCGCAACTTGTCACGTCAGCCTGCACCGGCGCTATACTGAAAACATCTCCTTACTG
TGAACCAAAATAAACCGAAC

Downstream 100 bases:

>100_bases
ACAACACTCATGAAACACATTATATCCGTTTTGGTTGAGAACAAGTTCGGCTCCCTGAACAGGGTGGCCTCCATGTTCAG
CGCCCGAGGGTTCAACCTCG

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MNVPGQTLNGSEIFFECLRRENVEYIFGYPGGALLKVYETLYDIKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGIANAYMDSSPMVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVRDVRELALTIRKAFYLATNGRPGP
VLVDMPKDILNASCEFQWPENIEIRGFKPTVKCHMNQVEKAALKISKAKKPLFYIGGGVISANASEELRKLAIQQNIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVNNCDLLIAVGARFDDRVTGKVETFATQAYKIHNDIDPTNVDKNIKVDL
PIVGDAKHFLSALLDEMPEATENREPWLQEIHKWQEQCPLAYETGDELLRTEFVIEETSKQTKGNAVVVTDVGQHQMWTS
QYYKFNNPRSIITSGGLGTMGYGLPASIGAAFGVTDRPIILFCGDGGFMMNVQELVTAVHYKIPVKIFLINNTFLGMVRQ
WQELFHKEQYSFTDLGNSNPDFVKVADAFGCRTSCAETPDQARQAIKEALAWNEGPFLVEFKVIKKDMVFPMVPAGGSIS
DMMLARLNPKTMV

Sequences:

>Translated_573_residues
MNVPGQTLNGSEIFFECLRRENVEYIFGYPGGALLKVYETLYDIKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGIANAYMDSSPMVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVRDVRELALTIRKAFYLATNGRPGP
VLVDMPKDILNASCEFQWPENIEIRGFKPTVKCHMNQVEKAALKISKAKKPLFYIGGGVISANASEELRKLAIQQNIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVNNCDLLIAVGARFDDRVTGKVETFATQAYKIHNDIDPTNVDKNIKVDL
PIVGDAKHFLSALLDEMPEATENREPWLQEIHKWQEQCPLAYETGDELLRTEFVIEETSKQTKGNAVVVTDVGQHQMWTS
QYYKFNNPRSIITSGGLGTMGYGLPASIGAAFGVTDRPIILFCGDGGFMMNVQELVTAVHYKIPVKIFLINNTFLGMVRQ
WQELFHKEQYSFTDLGNSNPDFVKVADAFGCRTSCAETPDQARQAIKEALAWNEGPFLVEFKVIKKDMVFPMVPAGGSIS
DMMLARLNPKTMV
>Mature_573_residues
MNVPGQTLNGSEIFFECLRRENVEYIFGYPGGALLKVYETLYDIKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGIANAYMDSSPMVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVRDVRELALTIRKAFYLATNGRPGP
VLVDMPKDILNASCEFQWPENIEIRGFKPTVKCHMNQVEKAALKISKAKKPLFYIGGGVISANASEELRKLAIQQNIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVNNCDLLIAVGARFDDRVTGKVETFATQAYKIHNDIDPTNVDKNIKVDL
PIVGDAKHFLSALLDEMPEATENREPWLQEIHKWQEQCPLAYETGDELLRTEFVIEETSKQTKGNAVVVTDVGQHQMWTS
QYYKFNNPRSIITSGGLGTMGYGLPASIGAAFGVTDRPIILFCGDGGFMMNVQELVTAVHYKIPVKIFLINNTFLGMVRQ
WQELFHKEQYSFTDLGNSNPDFVKVADAFGCRTSCAETPDQARQAIKEALAWNEGPFLVEFKVIKKDMVFPMVPAGGSIS
DMMLARLNPKTMV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=577, Percent_Identity=26.8630849220104, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI21361361, Length=572, Percent_Identity=24.6503496503496, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=44.9122807017544, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=42.831541218638, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=542, Percent_Identity=33.210332103321, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=26.007326007326, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788716, Length=590, Percent_Identity=26.1016949152542, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=25.4867256637168, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=25.4867256637168, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=577, Percent_Identity=24.0901213171577, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=46.5968586387435, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320123, Length=534, Percent_Identity=25.0936329588015, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320816, Length=499, Percent_Identity=23.6472945891784, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=522, Percent_Identity=23.7547892720307, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=527, Percent_Identity=23.719165085389, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=481, Percent_Identity=23.9085239085239, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922626, Length=482, Percent_Identity=26.7634854771784, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63310; Mature: 63310

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPGQTLNGSEIFFECLRRENVEYIFGYPGGALLKVYETLYDIKDIKHILVRHEQGAAH
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGIVLVTSGPGATNTVTGIANAYMDSSPMVVFTGQVPSSLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHCCCHHCCC
DIVGITRPITKHNFLVRDVRELALTIRKAFYLATNGRPGPVLVDMPKDILNASCEFQWPE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCCEECCCC
NIEIRGFKPTVKCHMNQVEKAALKISKAKKPLFYIGGGVISANASEELRKLAIQQNIPVT
CCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEE
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVNNCDLLIAVGARFDDRVTGKVETFATQA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
YKIHNDIDPTNVDKNIKVDLPIVGDAKHFLSALLDEMPEATENREPWLQEIHKWQEQCPL
EEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
AYETGDELLRTEFVIEETSKQTKGNAVVVTDVGQHQMWTSQYYKFNNPRSIITSGGLGTM
EECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEECCCCEEEECCCCCCC
GYGLPASIGAAFGVTDRPIILFCGDGGFMMNVQELVTAVHYKIPVKIFLINNTFLGMVRQ
CCCCCHHHCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHEECCEEEEEECCHHHHHHHH
WQELFHKEQYSFTDLGNSNPDFVKVADAFGCRTSCAETPDQARQAIKEALAWNEGPFLVE
HHHHHHHCCCCCCCCCCCCCCEEEEECHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
FKVIKKDMVFPMVPAGGSISDMMLARLNPKTMV
EEEECCCCCCEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNVPGQTLNGSEIFFECLRRENVEYIFGYPGGALLKVYETLYDIKDIKHILVRHEQGAAH
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGIVLVTSGPGATNTVTGIANAYMDSSPMVVFTGQVPSSLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHCCCHHCCC
DIVGITRPITKHNFLVRDVRELALTIRKAFYLATNGRPGPVLVDMPKDILNASCEFQWPE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCCEECCCC
NIEIRGFKPTVKCHMNQVEKAALKISKAKKPLFYIGGGVISANASEELRKLAIQQNIPVT
CCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEE
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVNNCDLLIAVGARFDDRVTGKVETFATQA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
YKIHNDIDPTNVDKNIKVDLPIVGDAKHFLSALLDEMPEATENREPWLQEIHKWQEQCPL
EEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
AYETGDELLRTEFVIEETSKQTKGNAVVVTDVGQHQMWTSQYYKFNNPRSIITSGGLGTM
EECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEECCCCEEEECCCCCCC
GYGLPASIGAAFGVTDRPIILFCGDGGFMMNVQELVTAVHYKIPVKIFLINNTFLGMVRQ
CCCCCHHHCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHEECCEEEEEECCHHHHHHHH
WQELFHKEQYSFTDLGNSNPDFVKVADAFGCRTSCAETPDQARQAIKEALAWNEGPFLVE
HHHHHHHCCCCCCCCCCCCCCEEEEECHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
FKVIKKDMVFPMVPAGGSISDMMLARLNPKTMV
EEEECCCCCCEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]