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The map label for this gene is acrA [H]

Identifier: 119356609

GI number: 119356609

Start: 887540

End: 888694

Strand: Reverse

Name: acrA [H]

Synonym: Cpha266_0777

Alternate gene names: 119356609

Gene position: 888694-887540 (Counterclockwise)

Preceding gene: 119356610

Following gene: 119356608

Centisome position: 28.36

GC content: 56.28

Gene sequence:

>1155_bases
ATGATGAAAAGGTCGTATATCATCGCCGGTTTGCTTGTCACCCTGATTGCTGGCTGGTACCTGTGGCCGAAGGGATCGTC
AGAGCAGCGTCAGGATTCCGGCATGAAACCGGAGGTCAGTGTCGTGACCATGAAACCCGAACTCCTCGTATTGACAAAAG
AGCTGCCGGGGCGGACTTCGGCTGTACGTATGGCAGAAATCCGGCCGCAGGTGAGCGGCATTGTTACGAAGCGCTTCTTC
GTGGAAGGAAGCCTTGTTAAACAGGGTCAGCAACTCTACCAGATCGACCCTGCGACCTATCAAGCCGCCTGTAACAGTGC
AAAAGCCAATCTCGGCAAAGCCGAGGCCAACGTGAAATCGGTTTTGGCAAAAGCAGCTCGCTACGAAGAACTGGTCAAAA
TCGGTGGCGTCAGCCGGCAGGAGTACGACGATGCAAAAGCGAACCTTGCCCAGGCGAGGGCGGATGTCGACATCGCCAGA
TCCGAAGTTTCCTCGGCCGGCATCAACCTCGACTACACCAGGGTCATGTCTCCGATTTCCGGACGAATCGGCCAATCGAA
GGTGACCGAGGGGGCTCTGGTCAATGCCAATCAAGCCACGCCCCTTGCTGTAGTGCAACAGCTCGACCAGATCTACGTGG
ACGTTTCCCAGTCGAGCGATGAACTGTTACAGCTGCGACAGCGCCATCAAGGCCAGGTTGGCGATCCCGGTGTCGAGAGT
GCTCCTGTCCAGCTTCTGGTGGACGGAAAACCCAAAGGACAACCCGGCACGTTAAAGTTCTCGGATGTAACGGTCAACCC
GAGCACAGGAACGGTCCTGTTGCGAATATTGTTCCCCAACCCCGGCAATGAAATCCTCCCCGGCATGTTCGTTCATGCAC
GTCTTGAACAGTGGAGAGACGAGCAGGCCATCCTCGTACCGCAGAAATCGGTCAGCAGAAGTGCGGACGGATCGGTTTCG
GTCTGGGTTGTCGGACCGGACGGCAAAGCAAACCCCCGCCCGATCATCGTTACGGAAGTTGTCGGAGACAGGTGGCTTGT
AAGCAGCGGACTCAAAGCAAGCGAGAAGGTCGTGTACGAAGGCATCATGAAAATCCAGCCGGGCATGGCGGTCAAAACCG
TCGAAGCAACACTGCCACCCGTGCAGGCTAAATAA

Upstream 100 bases:

>100_bases
GGAGCGTCTGCCCTTCCTGGAACAGATCCGAAAGATGTATATCGGTTATCCTGAAAGCCAACCGACAACCACACCAAACC
CATAAAAGGATACGTTATCA

Downstream 100 bases:

>100_bases
TCCATTACATTTCAACGCACCAGTACCATGTCCGGATTTTTTATCGAGCGACCCGTCTTTGCCTGGGTGATCTCCATCTG
TATCATGCTCGGCGGAATCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MMKRSYIIAGLLVTLIAGWYLWPKGSSEQRQDSGMKPEVSVVTMKPELLVLTKELPGRTSAVRMAEIRPQVSGIVTKRFF
VEGSLVKQGQQLYQIDPATYQAACNSAKANLGKAEANVKSVLAKAARYEELVKIGGVSRQEYDDAKANLAQARADVDIAR
SEVSSAGINLDYTRVMSPISGRIGQSKVTEGALVNANQATPLAVVQQLDQIYVDVSQSSDELLQLRQRHQGQVGDPGVES
APVQLLVDGKPKGQPGTLKFSDVTVNPSTGTVLLRILFPNPGNEILPGMFVHARLEQWRDEQAILVPQKSVSRSADGSVS
VWVVGPDGKANPRPIIVTEVVGDRWLVSSGLKASEKVVYEGIMKIQPGMAVKTVEATLPPVQAK

Sequences:

>Translated_384_residues
MMKRSYIIAGLLVTLIAGWYLWPKGSSEQRQDSGMKPEVSVVTMKPELLVLTKELPGRTSAVRMAEIRPQVSGIVTKRFF
VEGSLVKQGQQLYQIDPATYQAACNSAKANLGKAEANVKSVLAKAARYEELVKIGGVSRQEYDDAKANLAQARADVDIAR
SEVSSAGINLDYTRVMSPISGRIGQSKVTEGALVNANQATPLAVVQQLDQIYVDVSQSSDELLQLRQRHQGQVGDPGVES
APVQLLVDGKPKGQPGTLKFSDVTVNPSTGTVLLRILFPNPGNEILPGMFVHARLEQWRDEQAILVPQKSVSRSADGSVS
VWVVGPDGKANPRPIIVTEVVGDRWLVSSGLKASEKVVYEGIMKIQPGMAVKTVEATLPPVQAK
>Mature_384_residues
MMKRSYIIAGLLVTLIAGWYLWPKGSSEQRQDSGMKPEVSVVTMKPELLVLTKELPGRTSAVRMAEIRPQVSGIVTKRFF
VEGSLVKQGQQLYQIDPATYQAACNSAKANLGKAEANVKSVLAKAARYEELVKIGGVSRQEYDDAKANLAQARADVDIAR
SEVSSAGINLDYTRVMSPISGRIGQSKVTEGALVNANQATPLAVVQQLDQIYVDVSQSSDELLQLRQRHQGQVGDPGVES
APVQLLVDGKPKGQPGTLKFSDVTVNPSTGTVLLRILFPNPGNEILPGMFVHARLEQWRDEQAILVPQKSVSRSADGSVS
VWVVGPDGKANPRPIIVTEVVGDRWLVSSGLKASEKVVYEGIMKIQPGMAVKTVEATLPPVQAK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=50.8152173913043, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=47.5274725274725, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1789929, Length=370, Percent_Identity=43.2432432432432, Blast_Score=290, Evalue=7e-80,
Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=27.639751552795, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081796, Length=264, Percent_Identity=26.8939393939394, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41475; Mature: 41475

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRSYIIAGLLVTLIAGWYLWPKGSSEQRQDSGMKPEVSVVTMKPELLVLTKELPGRTS
CCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCH
AVRMAEIRPQVSGIVTKRFFVEGSLVKQGQQLYQIDPATYQAACNSAKANLGKAEANVKS
HEEHHHHCHHHHHHHHHHHEECCHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHH
VLAKAARYEELVKIGGVSRQEYDDAKANLAQARADVDIARSEVSSAGINLDYTRVMSPIS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GRIGQSKVTEGALVNANQATPLAVVQQLDQIYVDVSQSSDELLQLRQRHQGQVGDPGVES
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCC
APVQLLVDGKPKGQPGTLKFSDVTVNPSTGTVLLRILFPNPGNEILPGMFVHARLEQWRD
CCEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCC
EQAILVPQKSVSRSADGSVSVWVVGPDGKANPRPIIVTEVVGDRWLVSSGLKASEKVVYE
CCEEEECCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEHHCCCCHHHHHHHH
GIMKIQPGMAVKTVEATLPPVQAK
HHHHCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MMKRSYIIAGLLVTLIAGWYLWPKGSSEQRQDSGMKPEVSVVTMKPELLVLTKELPGRTS
CCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCH
AVRMAEIRPQVSGIVTKRFFVEGSLVKQGQQLYQIDPATYQAACNSAKANLGKAEANVKS
HEEHHHHCHHHHHHHHHHHEECCHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHH
VLAKAARYEELVKIGGVSRQEYDDAKANLAQARADVDIARSEVSSAGINLDYTRVMSPIS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GRIGQSKVTEGALVNANQATPLAVVQQLDQIYVDVSQSSDELLQLRQRHQGQVGDPGVES
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCC
APVQLLVDGKPKGQPGTLKFSDVTVNPSTGTVLLRILFPNPGNEILPGMFVHARLEQWRD
CCEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCC
EQAILVPQKSVSRSADGSVSVWVVGPDGKANPRPIIVTEVVGDRWLVSSGLKASEKVVYE
CCEEEECCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEHHCCCCHHHHHHHH
GIMKIQPGMAVKTVEATLPPVQAK
HHHHCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]