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The map label for this gene is oprM [H]

Identifier: 119356607

GI number: 119356607

Start: 882925

End: 884355

Strand: Reverse

Name: oprM [H]

Synonym: Cpha266_0775

Alternate gene names: 119356607

Gene position: 884355-882925 (Counterclockwise)

Preceding gene: 119356608

Following gene: 119356605

Centisome position: 28.22

GC content: 57.72

Gene sequence:

>1431_bases
ATGAACACTAAGCTGCTGCTTCCTTTATGCCTGGCAGCAACCACCCTGACGGCCTGTTCGCTTGAGCCTGCTTATGTACG
CCCAACGGCACCCGTCACCACGCAGTGGCCGGTGGCTTCCGGAATCGGGACAACGACCTCCACGGCACCGGACTCCGGAG
GACAGCCGCGTACCACCGATATCGGATGGCGGAGCATGTTCAGGTCGCCAAAACTGCAAAGCCTTATCGAAGAATCACTT
GCCAACAACCGCGACCTGAGGATAGCCACCCTCAACATCGAGGTAGCCCGCAACACCTGGCGCATCCAGCGTTCGGATCT
CCTTCCATCGATTAACGCCTCGGGAAGCTTCACACGCCAGGAGTTATCAAAAAGCACGAGCGGCACCGGAAATAAGGAAA
TCACCTCGACATATACCGCAAACATCGGCACGACGGCATATGAACTGGACCTTTTCGGCCGTGTACGCAGCCTCAGTAAT
CGCGCCATAAACCAGTATCTTGCTACCGAAGAGGGCCGCAAAGCCGCCCAGACAAGCCTGATCGCTGAAGTCGCCAACGC
CTATCTCACCTACCTTGCCGATGTCGAACTGCTCCGATTGACCGAAAGCACGCTCAGGACAAGGCAGGATGCGTACGATC
TCATCAAACGCAGCTACGATTTAGGGGCAAAGTCCCGACTCGATGTAGCACAGTCCGCAACACTGGCAGAATCGGCCCGA
GCAAGCCGTGCGCAATATCAACGTCAGGTGGAACAGGACAAGAACGCCCTGACCCTGCTGGTCGGAAAGGAGATAGATCC
GAAGCTCCTTGAACCCCAGCGCCTGACCGATGTCCAGGTGCTGGACACGCTCCCCGCAGGCATTCCCTCCGTCGTCCTGC
TCGATCGACCGGACGTACGGCAGGCGGAATACGCACTGAAGGCAGAAAACGCCAACATCGGAGCAGCACGGGCTTTATTC
TTCCCCAGCATAAGTCTTACCGGCTCAACCGGGTTCGCCAGCGCCAGCCTGTCGGATCTGTTCGTGACCCGATCAAGCGG
TATTTGGAGCTTCGCCCCGCAGATAACGCTCCCGATCTTCCAGAGCGGCCGTTTGCGCGCCAACCTCAAACTGGCTGAAA
CCAACCGGGATATTGCCGTCGCCAAATACGAAAAAGCTATACAGACCGCATTCCGGGAGGTCTCCGACGCACTGGTCGCC
CGAGCGACCTACACCGAACAGTTGCATGCCCAGCGAGCTCTGGTCACAGAGAGCGATCAAGCCCGGACCATCACGAAAGC
GCGTTACGACCATGGCATCGACAGCTATTTCGCGCTGCTTGACGCCCAACGCTCCCTTTTCGAGGCTCAGCAGAACGAAA
TTCTGATCCATCAGCAGACTCTTGCCAACCTGATCGACCTGTACAAAGCCCTCGGAGGCGGGTGGCAATGA

Upstream 100 bases:

>100_bases
ATCTTCTTTGTGCCTCTCTTCTTCATCCTCGTGCAATCCCGGTTTGCCCGAAAGACGGCAACCGACACTGCAGTAAACCA
AAACGACAAGGGATCGAACC

Downstream 100 bases:

>100_bases
CAAGGCACTTCCCCTTACGCACGACGCTGTCAGTTTCAGGCGACCCAAAAAGATAGCGGGACGCTCATAGACGGGGGCAC
GTCCCGAGCAGCAAGGGCCA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNTKLLLPLCLAATTLTACSLEPAYVRPTAPVTTQWPVASGIGTTTSTAPDSGGQPRTTDIGWRSMFRSPKLQSLIEESL
ANNRDLRIATLNIEVARNTWRIQRSDLLPSINASGSFTRQELSKSTSGTGNKEITSTYTANIGTTAYELDLFGRVRSLSN
RAINQYLATEEGRKAAQTSLIAEVANAYLTYLADVELLRLTESTLRTRQDAYDLIKRSYDLGAKSRLDVAQSATLAESAR
ASRAQYQRQVEQDKNALTLLVGKEIDPKLLEPQRLTDVQVLDTLPAGIPSVVLLDRPDVRQAEYALKAENANIGAARALF
FPSISLTGSTGFASASLSDLFVTRSSGIWSFAPQITLPIFQSGRLRANLKLAETNRDIAVAKYEKAIQTAFREVSDALVA
RATYTEQLHAQRALVTESDQARTITKARYDHGIDSYFALLDAQRSLFEAQQNEILIHQQTLANLIDLYKALGGGWQ

Sequences:

>Translated_476_residues
MNTKLLLPLCLAATTLTACSLEPAYVRPTAPVTTQWPVASGIGTTTSTAPDSGGQPRTTDIGWRSMFRSPKLQSLIEESL
ANNRDLRIATLNIEVARNTWRIQRSDLLPSINASGSFTRQELSKSTSGTGNKEITSTYTANIGTTAYELDLFGRVRSLSN
RAINQYLATEEGRKAAQTSLIAEVANAYLTYLADVELLRLTESTLRTRQDAYDLIKRSYDLGAKSRLDVAQSATLAESAR
ASRAQYQRQVEQDKNALTLLVGKEIDPKLLEPQRLTDVQVLDTLPAGIPSVVLLDRPDVRQAEYALKAENANIGAARALF
FPSISLTGSTGFASASLSDLFVTRSSGIWSFAPQITLPIFQSGRLRANLKLAETNRDIAVAKYEKAIQTAFREVSDALVA
RATYTEQLHAQRALVTESDQARTITKARYDHGIDSYFALLDAQRSLFEAQQNEILIHQQTLANLIDLYKALGGGWQ
>Mature_476_residues
MNTKLLLPLCLAATTLTACSLEPAYVRPTAPVTTQWPVASGIGTTTSTAPDSGGQPRTTDIGWRSMFRSPKLQSLIEESL
ANNRDLRIATLNIEVARNTWRIQRSDLLPSINASGSFTRQELSKSTSGTGNKEITSTYTANIGTTAYELDLFGRVRSLSN
RAINQYLATEEGRKAAQTSLIAEVANAYLTYLADVELLRLTESTLRTRQDAYDLIKRSYDLGAKSRLDVAQSATLAESAR
ASRAQYQRQVEQDKNALTLLVGKEIDPKLLEPQRLTDVQVLDTLPAGIPSVVLLDRPDVRQAEYALKAENANIGAARALF
FPSISLTGSTGFASASLSDLFVTRSSGIWSFAPQITLPIFQSGRLRANLKLAETNRDIAVAKYEKAIQTAFREVSDALVA
RATYTEQLHAQRALVTESDQARTITKARYDHGIDSYFALLDAQRSLFEAQQNEILIHQQTLANLIDLYKALGGGWQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=39.3684210526316, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI2367348, Length=500, Percent_Identity=26.4, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52253; Mature: 52253

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKLLLPLCLAATTLTACSLEPAYVRPTAPVTTQWPVASGIGTTTSTAPDSGGQPRTTD
CCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IGWRSMFRSPKLQSLIEESLANNRDLRIATLNIEVARNTWRIQRSDLLPSINASGSFTRQ
HHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEHHHCCCCCCCCCCHHHH
ELSKSTSGTGNKEITSTYTANIGTTAYELDLFGRVRSLSNRAINQYLATEEGRKAAQTSL
HHHHCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEVANAYLTYLADVELLRLTESTLRTRQDAYDLIKRSYDLGAKSRLDVAQSATLAESAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASRAQYQRQVEQDKNALTLLVGKEIDPKLLEPQRLTDVQVLDTLPAGIPSVVLLDRPDVR
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCH
QAEYALKAENANIGAARALFFPSISLTGSTGFASASLSDLFVTRSSGIWSFAPQITLPIF
HHHHHHCCCCCCCCHHHHHEECCEEECCCCCCCCCCHHHHEEECCCCCEEECCCEEEEEE
QSGRLRANLKLAETNRDIAVAKYEKAIQTAFREVSDALVARATYTEQLHAQRALVTESDQ
CCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARTITKARYDHGIDSYFALLDAQRSLFEAQQNEILIHQQTLANLIDLYKALGGGWQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTKLLLPLCLAATTLTACSLEPAYVRPTAPVTTQWPVASGIGTTTSTAPDSGGQPRTTD
CCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IGWRSMFRSPKLQSLIEESLANNRDLRIATLNIEVARNTWRIQRSDLLPSINASGSFTRQ
HHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEHHHCCCCCCCCCCHHHH
ELSKSTSGTGNKEITSTYTANIGTTAYELDLFGRVRSLSNRAINQYLATEEGRKAAQTSL
HHHHCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEVANAYLTYLADVELLRLTESTLRTRQDAYDLIKRSYDLGAKSRLDVAQSATLAESAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASRAQYQRQVEQDKNALTLLVGKEIDPKLLEPQRLTDVQVLDTLPAGIPSVVLLDRPDVR
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCH
QAEYALKAENANIGAARALFFPSISLTGSTGFASASLSDLFVTRSSGIWSFAPQITLPIF
HHHHHHCCCCCCCCHHHHHEECCEEECCCCCCCCCCHHHHEEECCCCCEEECCCEEEEEE
QSGRLRANLKLAETNRDIAVAKYEKAIQTAFREVSDALVARATYTEQLHAQRALVTESDQ
CCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARTITKARYDHGIDSYFALLDAQRSLFEAQQNEILIHQQTLANLIDLYKALGGGWQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]