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The map label for this gene is gcvH

Identifier: 119356409

GI number: 119356409

Start: 644478

End: 644861

Strand: Direct

Name: gcvH

Synonym: Cpha266_0573

Alternate gene names: 119356409

Gene position: 644478-644861 (Clockwise)

Preceding gene: 119356408

Following gene: 119356410

Centisome position: 20.56

GC content: 50.0

Gene sequence:

>384_bases
ATGAATATTCCTGAAGATCTTCGCTATACCAAGGACCACGAATGGGTCAAGCTGCTTGACGATGGCAGCACCGCGCTGGC
CGGTATTACTGACTTTGCCCAGTCTGAACTGGGTGATATTGTTTTTGTTGAGCTTAAAGCTCCAGGAACAAAGCTGAAAG
CTCATGAGGTTTTTGGAACTGTTGAGGCAGTTAAAACTGTCGCTGATCTGTTTGCTCCGATTGCCGGAGAGCTTGTCGGG
ATCAACGAGGCCCTCGATGCTGCTGAAGTTGTCAATACTGACCCTTATGGGGAGGGTTGGCTCGTGAAGATAAAGGTCGA
TGATGCAAAAGCGGTAGAAGAGCTGCTTGATGCCGCCTCTTACCGCCAACTGATTGGCGCGTGA

Upstream 100 bases:

>100_bases
TTCCTCAACAGCAGTAATTTCGCCGCTTGTTTCAGCTCTTTAAAAAAAATGTTACCTTCAGATTCACTTTTGTTCCAACC
CTAATAACAACTTCGCCGCC

Downstream 100 bases:

>100_bases
CACTTTTTTAAACCTCTGTTTTTCATTACATAGCAATGCCATTCATTGTCAATACCGATCAGGATACCAGAGAGATGCTT
TCAGTTTTGGGTGCAGCTTC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNIPEDLRYTKDHEWVKLLDDGSTALAGITDFAQSELGDIVFVELKAPGTKLKAHEVFGTVEAVKTVADLFAPIAGELVG
INEALDAAEVVNTDPYGEGWLVKIKVDDAKAVEELLDAASYRQLIGA

Sequences:

>Translated_127_residues
MNIPEDLRYTKDHEWVKLLDDGSTALAGITDFAQSELGDIVFVELKAPGTKLKAHEVFGTVEAVKTVADLFAPIAGELVG
INEALDAAEVVNTDPYGEGWLVKIKVDDAKAVEELLDAASYRQLIGA
>Mature_127_residues
MNIPEDLRYTKDHEWVKLLDDGSTALAGITDFAQSELGDIVFVELKAPGTKLKAHEVFGTVEAVKTVADLFAPIAGELVG
INEALDAAEVVNTDPYGEGWLVKIKVDDAKAVEELLDAASYRQLIGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=36.9747899159664, Blast_Score=93, Evalue=5e-20,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=36.1344537815126, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=44.8, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=42.7350427350427, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=35.8974358974359, Blast_Score=88, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=39.6694214876033, Blast_Score=92, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CHLPD (A1BE02)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911053.1
- ProteinModelPortal:   A1BE02
- SMR:   A1BE02
- STRING:   A1BE02
- GeneID:   4569168
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0573
- NMPDR:   fig|290317.7.peg.601
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13671; Mature: 13671

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPEDLRYTKDHEWVKLLDDGSTALAGITDFAQSELGDIVFVELKAPGTKLKAHEVFGT
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
VEAVKTVADLFAPIAGELVGINEALDAAEVVNTDPYGEGWLVKIKVDDAKAVEELLDAAS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHH
YRQLIGA
HHHHHCC
>Mature Secondary Structure
MNIPEDLRYTKDHEWVKLLDDGSTALAGITDFAQSELGDIVFVELKAPGTKLKAHEVFGT
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
VEAVKTVADLFAPIAGELVGINEALDAAEVVNTDPYGEGWLVKIKVDDAKAVEELLDAAS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHH
YRQLIGA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA