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The map label for this gene is aegA [H]

Identifier: 119356326

GI number: 119356326

Start: 536306

End: 537754

Strand: Reverse

Name: aegA [H]

Synonym: Cpha266_0489

Alternate gene names: 119356326

Gene position: 537754-536306 (Counterclockwise)

Preceding gene: 119356327

Following gene: 119356325

Centisome position: 17.16

GC content: 51.21

Gene sequence:

>1449_bases
ATGAATGAAAAAACTATCTCAAACAAAGAGCGTCTTGCCATTCCCAGACAAAAAATGCCTGCACAGGACCCGAACGAACG
GGTTACCAATTTCAGAGAGGTAAATCTCGGGTATACCCCTGAACTTGCACGGATGGAGGCGTTACGATGTATTCAGTGCA
AGGACCCGGTCTGCATTGCGGGGTGCCCGGTCAACATCAAAATCGACCAGTTCATCAAGATGGTAGCCGATGGAGATTTT
CTCGGCGCAGCAAAAAAAATAAAAGAGGACAACGTTTTGCCGGCAATCTGCGGAAGAGTCTGCCCGCAGGAAGACCAGTG
CGAAAAAGTCTGTGTCATAGGAAAAAAACATGAACCGGTTGCCATCGGTCACCTTGAACGTTTTGTCGCCGACTACGAAC
GCCAAACCGGCCATGTCGAGATCCCTGAAGTCAAACCGCCAACCGGCAAAAAAATCGCAATTATCGGCAGCGGTCCGGCA
GGGCTGAGCTGTGCAAACGATCTGGTACGTCTTGGGCATGAGATAGTCGTATTTGAAGCGCTGCACGAACTTGGCGGCGT
GCTCATGTACGGGATTCCCGAGTTTCGTCTTCCCAAAGAGATTGTCCGGCAGGAACTCGATGGCCTGAAACAGATGGGGG
TCTCATTCCAGACCAATGTTGTGGTTGGACGATCGGTGACGATCGACGAGCTTATCGAAGAGGAGGGATTCAATGCCGTT
TTCATCGGCGTCGGAGCCGGACTGCCCTGGTTTATGGGCATCCCGGGAGAAAACCTTGCAGGAGTACTTTCAGCCAATGA
ATTCCTCACCAGGGTAAATCTCATGAAAGCTTACGATTTCCCGGAAAGCGACACCCCTGTATTCCAGTGCAAGAACAGAA
ACATTGCTGTTTTCGGAGGAGGCAACACCGCTATGGATGCTGTCCGTACAGCCAAAAGACTGGGCGCTGAACACGCCTAT
ATCATTTACCGCCGCTCCGAAGCCGAAATGCCTGCCCGCCACGAAGAGATTCACCATGCAAAAGAAGAGGGTGTTGAGTT
CCTGCTTCTCATGAACCCCATTGCATTTATCGGTAATGACCAGCAGCAGTTAACCGGCGTACGCTGCCTCCGGATGGAAC
TTGGCGAACCCGATGATTCGGGAAGAAGACGACCGGTTCCAATCGAGGGATCGGAATTTCTGCTTCCCCTCGATATGGCC
GTCATCTCGATCGGCAACGGCTCAAATCCGCTTATCAAGCAGACAACTCCGGATATAGAGGTCAGCAAACGAGATACGGT
TGTCGTTGACATCAACACCATGGCCACCTCAAAAAATATGGTTTATGCCGGTGGAGATATCGTTACCGGAGGAGCAACCG
TTATTCTTGCCATGGGTGCAGGACGTACAGCGGCAAAAGCAATTCACGAAAAGCTCATGGGCGAATCACATGGTTTTGAC
GAGTGGTAA

Upstream 100 bases:

>100_bases
TTCCCTGGATGCTCTCTGCCATTGATTCCCGACAAAACGGAGATCAATGGCAGAGTTTATCATCGAAACAAGTTTTAACG
CATAGTAAAACAGAATTATT

Downstream 100 bases:

>100_bases
AAAGCATATAACTGCCAGACAATCAACCCCTATACCAGCAACAACATGAGTGAGATATACCGTTTCGGCGTATCCCTCGA
CAAGTCCCTGATCGAAGCAT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNEKTISNKERLAIPRQKMPAQDPNERVTNFREVNLGYTPELARMEALRCIQCKDPVCIAGCPVNIKIDQFIKMVADGDF
LGAAKKIKEDNVLPAICGRVCPQEDQCEKVCVIGKKHEPVAIGHLERFVADYERQTGHVEIPEVKPPTGKKIAIIGSGPA
GLSCANDLVRLGHEIVVFEALHELGGVLMYGIPEFRLPKEIVRQELDGLKQMGVSFQTNVVVGRSVTIDELIEEEGFNAV
FIGVGAGLPWFMGIPGENLAGVLSANEFLTRVNLMKAYDFPESDTPVFQCKNRNIAVFGGGNTAMDAVRTAKRLGAEHAY
IIYRRSEAEMPARHEEIHHAKEEGVEFLLLMNPIAFIGNDQQQLTGVRCLRMELGEPDDSGRRRPVPIEGSEFLLPLDMA
VISIGNGSNPLIKQTTPDIEVSKRDTVVVDINTMATSKNMVYAGGDIVTGGATVILAMGAGRTAAKAIHEKLMGESHGFD
EW

Sequences:

>Translated_482_residues
MNEKTISNKERLAIPRQKMPAQDPNERVTNFREVNLGYTPELARMEALRCIQCKDPVCIAGCPVNIKIDQFIKMVADGDF
LGAAKKIKEDNVLPAICGRVCPQEDQCEKVCVIGKKHEPVAIGHLERFVADYERQTGHVEIPEVKPPTGKKIAIIGSGPA
GLSCANDLVRLGHEIVVFEALHELGGVLMYGIPEFRLPKEIVRQELDGLKQMGVSFQTNVVVGRSVTIDELIEEEGFNAV
FIGVGAGLPWFMGIPGENLAGVLSANEFLTRVNLMKAYDFPESDTPVFQCKNRNIAVFGGGNTAMDAVRTAKRLGAEHAY
IIYRRSEAEMPARHEEIHHAKEEGVEFLLLMNPIAFIGNDQQQLTGVRCLRMELGEPDDSGRRRPVPIEGSEFLLPLDMA
VISIGNGSNPLIKQTTPDIEVSKRDTVVVDINTMATSKNMVYAGGDIVTGGATVILAMGAGRTAAKAIHEKLMGESHGFD
EW
>Mature_482_residues
MNEKTISNKERLAIPRQKMPAQDPNERVTNFREVNLGYTPELARMEALRCIQCKDPVCIAGCPVNIKIDQFIKMVADGDF
LGAAKKIKEDNVLPAICGRVCPQEDQCEKVCVIGKKHEPVAIGHLERFVADYERQTGHVEIPEVKPPTGKKIAIIGSGPA
GLSCANDLVRLGHEIVVFEALHELGGVLMYGIPEFRLPKEIVRQELDGLKQMGVSFQTNVVVGRSVTIDELIEEEGFNAV
FIGVGAGLPWFMGIPGENLAGVLSANEFLTRVNLMKAYDFPESDTPVFQCKNRNIAVFGGGNTAMDAVRTAKRLGAEHAY
IIYRRSEAEMPARHEEIHHAKEEGVEFLLLMNPIAFIGNDQQQLTGVRCLRMELGEPDDSGRRRPVPIEGSEFLLPLDMA
VISIGNGSNPLIKQTTPDIEVSKRDTVVVDINTMATSKNMVYAGGDIVTGGATVILAMGAGRTAAKAIHEKLMGESHGFD
EW

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=31.9383259911894, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=41.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=452, Percent_Identity=38.2743362831858, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=36.8888888888889, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=37.1937639198218, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=32.0627802690583, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789243, Length=455, Percent_Identity=31.4285714285714, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=29.3522267206478, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71984108, Length=465, Percent_Identity=29.8924731182796, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=164, Percent_Identity=30.4878048780488, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=482, Percent_Identity=29.4605809128631, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=28.1893004115226, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=28.1893004115226, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=28.1893004115226, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=28.1893004115226, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24640763, Length=444, Percent_Identity=31.5315315315315, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI18858217, Length=444, Percent_Identity=31.5315315315315, Blast_Score=196, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52796; Mature: 52796

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKTISNKERLAIPRQKMPAQDPNERVTNFREVNLGYTPELARMEALRCIQCKDPVCIA
CCCCCCCCCHHCCCCHHHCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GCPVNIKIDQFIKMVADGDFLGAAKKIKEDNVLPAICGRVCPQEDQCEKVCVIGKKHEPV
CCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCE
AIGHLERFVADYERQTGHVEIPEVKPPTGKKIAIIGSGPAGLSCANDLVRLGHEIVVFEA
EHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHH
LHELGGVLMYGIPEFRLPKEIVRQELDGLKQMGVSFQTNVVVGRSVTIDELIEEEGFNAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCEE
FIGVGAGLPWFMGIPGENLAGVLSANEFLTRVNLMKAYDFPESDTPVFQCKNRNIAVFGG
EEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEC
GNTAMDAVRTAKRLGAEHAYIIYRRSEAEMPARHEEIHHAKEEGVEFLLLMNPIAFIGND
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCHHEECCC
QQQLTGVRCLRMELGEPDDSGRRRPVPIEGSEFLLPLDMAVISIGNGSNPLIKQTTPDIE
HHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCCCE
VSKRDTVVVDINTMATSKNMVYAGGDIVTGGATVILAMGAGRTAAKAIHEKLMGESHGFD
ECCCCEEEEEEEEEECCCCEEEECCCEEECCEEEEEEECCCHHHHHHHHHHHCCCCCCCC
EW
CC
>Mature Secondary Structure
MNEKTISNKERLAIPRQKMPAQDPNERVTNFREVNLGYTPELARMEALRCIQCKDPVCIA
CCCCCCCCCHHCCCCHHHCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GCPVNIKIDQFIKMVADGDFLGAAKKIKEDNVLPAICGRVCPQEDQCEKVCVIGKKHEPV
CCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCE
AIGHLERFVADYERQTGHVEIPEVKPPTGKKIAIIGSGPAGLSCANDLVRLGHEIVVFEA
EHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHH
LHELGGVLMYGIPEFRLPKEIVRQELDGLKQMGVSFQTNVVVGRSVTIDELIEEEGFNAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCEE
FIGVGAGLPWFMGIPGENLAGVLSANEFLTRVNLMKAYDFPESDTPVFQCKNRNIAVFGG
EEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEC
GNTAMDAVRTAKRLGAEHAYIIYRRSEAEMPARHEEIHHAKEEGVEFLLLMNPIAFIGND
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCHHEECCC
QQQLTGVRCLRMELGEPDDSGRRRPVPIEGSEFLLPLDMAVISIGNGSNPLIKQTTPDIE
HHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCCCE
VSKRDTVVVDINTMATSKNMVYAGGDIVTGGATVILAMGAGRTAAKAIHEKLMGESHGFD
ECCCCEEEEEEEEEECCCCEEEECCCEEECCEEEEEEECCCHHHHHHHHHHHCCCCCCCC
EW
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]