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The map label for this gene is hisI

Identifier: 119356316

GI number: 119356316

Start: 527939

End: 528352

Strand: Reverse

Name: hisI

Synonym: Cpha266_0479

Alternate gene names: 119356316

Gene position: 528352-527939 (Counterclockwise)

Preceding gene: 119356321

Following gene: 119356315

Centisome position: 16.86

GC content: 46.14

Gene sequence:

>414_bases
ATGAGCGAGAATGAAGACCTGCAGAAGAGCTTTCTTGATACCGTCAAATATGATGAAAAAGGGCTGGTTCCGGCCATTGT
CCAGGATCATGAAACAGGAAAAGTGCTGATGATGGCATGGATGAACCATGAAAGCCTTCTGATGACGCTCGAAAAAAAGA
AAGCCTGCTACTGGAGCAGAAGCCGCCAGAAACTCTGGCTCAAGGGAGAATCTTCAGGAAACATGCAGGATGTGCACGAC
ATTCTCATTGACTGCGACGGCGACACCATTCTGCTGAAAGTATCCCAAAAAGGCGGAGCGTGCCATGTCGGCTATCACTC
CTGTTTCTATCGAAAAGTAAAAGAAACTCTTGATATGGAAATCTGTGACACCCTGATGTTCAATCCGGATGACGTTTACG
GAAAAAAAAGCTGA

Upstream 100 bases:

>100_bases
CCTTGGTTTTTCCATAATTTTATGAATCTTTAAAAACAAATTTCCCGGTAACCCAGGCGACAGCGATTATCGCCAGAATA
ACATTTAACCATACACGGCA

Downstream 100 bases:

>100_bases
TATGCTGATGAGCCACCCAAAAGAGACCGCCAAATCCCTGAACCAGACAAAGTCCCGTTCATCAATTCGGGAGATGTTTG
ACGAGGTTGCCCCAACCTAC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSENEDLQKSFLDTVKYDEKGLVPAIVQDHETGKVLMMAWMNHESLLMTLEKKKACYWSRSRQKLWLKGESSGNMQDVHD
ILIDCDGDTILLKVSQKGGACHVGYHSCFYRKVKETLDMEICDTLMFNPDDVYGKKS

Sequences:

>Translated_137_residues
MSENEDLQKSFLDTVKYDEKGLVPAIVQDHETGKVLMMAWMNHESLLMTLEKKKACYWSRSRQKLWLKGESSGNMQDVHD
ILIDCDGDTILLKVSQKGGACHVGYHSCFYRKVKETLDMEICDTLMFNPDDVYGKKS
>Mature_136_residues
SENEDLQKSFLDTVKYDEKGLVPAIVQDHETGKVLMMAWMNHESLLMTLEKKKACYWSRSRQKLWLKGESSGNMQDVHDI
LIDCDGDTILLKVSQKGGACHVGYHSCFYRKVKETLDMEICDTLMFNPDDVYGKKS

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=102, Percent_Identity=48.0392156862745, Blast_Score=97, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319819, Length=105, Percent_Identity=35.2380952380952, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_CHLPD (A1BDQ9)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910960.1
- ProteinModelPortal:   A1BDQ9
- SMR:   A1BDQ9
- STRING:   A1BDQ9
- GeneID:   4568523
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0479
- NMPDR:   fig|290317.7.peg.496
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 15743; Mature: 15612

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENEDLQKSFLDTVKYDEKGLVPAIVQDHETGKVLMMAWMNHESLLMTLEKKKACYWSR
CCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHCC
SRQKLWLKGESSGNMQDVHDILIDCDGDTILLKVSQKGGACHVGYHSCFYRKVKETLDME
CCCEEEEECCCCCCHHHHEEEEEECCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHCHH
ICDTLMFNPDDVYGKKS
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SENEDLQKSFLDTVKYDEKGLVPAIVQDHETGKVLMMAWMNHESLLMTLEKKKACYWSR
CCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHCC
SRQKLWLKGESSGNMQDVHDILIDCDGDTILLKVSQKGGACHVGYHSCFYRKVKETLDME
CCCEEEEECCCCCCHHHHEEEEEECCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHCHH
ICDTLMFNPDDVYGKKS
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA